Gender-specific effect of Pro12Ala polymorphism in peroxisome proliferator-activated receptor gamma-2 gene on obesity risk and leptin levels in a Tunisian population.

abstract：:Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifically bound to a appro...

journal_title：Clinical biochemistry

authors： Palaszynski EW,Donnelly JG,Soldin SJ

更新日期：1991-02-01 00:00:00

abstract：:Erythrocyte morphology, kinematic viscosity, and membrane lipid composition have not been well documented in heterozygous hypobetalipoproteinemia (HBL). In our study of nine subjects with HBL from two kindreds, three presented with schistocytes (3-6%) while two of them also showed acanthocytosis (25-35%) on their peri...

journal_title：Clinical biochemistry

authors： Gheeraert P,De Buyzere M,Delanghe J,De Scheerder I,Bury J,Rosseneu M

更新日期：1988-12-01 00:00:00

abstract：OBJECTIVES:Hepatitis B virus (HBV) can result in asymptomatic carrier (AsC) state or chronic inflammation of liver, which depends on the host immunity. We therefore investigated the peptidomic profiling in the process of HBV infection. DESIGN AND METHODS:In this study, serum from 116 HBV infected (AsC and chronic hepa...

journal_title：Clinical biochemistry

authors： Tian L,Wang Y,Xu D,Gao Y,Wen X,Tian Y

更新日期：2014-01-01 00:00:00

abstract：OBJECTIVES:Despite the evidence that both homocysteine and cysteine are important risk factors for vascular disease and atherosclerosis no information are reported about their effective amount in plaque and on the relationship with the other low molecular weight thiols. DESIGN AND METHODS:We used capillary electrophor...

journal_title：Clinical biochemistry

authors： Zinellu A,Lepedda A Jr,Sotgia S,Zinellu E,Scanu B,Turrini F,Spirito R,Deiana L,Formato M,Carru C

更新日期：2009-06-01 00:00:00

abstract：OBJECTIVES:In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for...

journal_title：Clinical biochemistry

authors： Albarhani AA,Collier F,Greaves RF,Ponsonby AL,Allen KJ,Vuillermin PJ,Roche P,Clarke MW,BIS Steering Committee.

更新日期：2015-11-01 00:00:00

abstract：:To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those...

journal_title：Clinical biochemistry

authors： Orzechowski A,Petrides V,Scopp R

更新日期：2018-07-01 00:00:00

abstract：OBJECTIVES:Sweat chloride test is the gold standard test for cystic fibrosis (CF) diagnosis. In 2014 the Istituto Superiore di Sanità established the Italian pilot external quality assessment program for CF sweat test (IEQA-ST). DESIGN AND METHODS:Ten laboratories, included among the 33 Italian CF Referral Centers, we...

journal_title：Clinical biochemistry

authors： Salvatore M,Floridia G,Amato A,Censi F,Carta C,de Stefano MC,Ferrari G,Tosto F,Capoluongo E,Caruso U,Castaldo G,Cirilli N,Corbetta C,Padoan R,Raia V,Taruscio D

更新日期：2016-05-01 00:00:00

abstract：OBJECTIVES:The non-invasive differentiation of ischemic and non-ischemic acute heart failure (AHF) not resulting from acute myocardial infarction is difficult and has therapeutic and prognostic implications. The aim of this study was to assess whether plasma B-type natriuretic peptide (BNP) can identify ischemic etiolo...

journal_title：Clinical biochemistry

authors： Kim SE,Park S,Kim JK,Kim SG,Kim HJ,Song YR

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVES:To evaluate the analytical performance of the testosterone assay performed on the Bayer immuno 1 system from Bayer Corporation. DESIGN AND METHODS:The precision was measured using three Bayer TESTpoint Ligand controls, three Medical Decision Pools and the Bayer SETpoint Testosterone calibrators. The lineari...

journal_title：Clinical biochemistry

authors： Lévesque A,Letellier M,Swirski C,Lee C,Grant A

更新日期：1998-02-01 00:00:00

abstract：:We describe a simple and reliable radioimmunoassay (RIA) method for the measurement of human placental lactogen (hPL) in undiluted serum. Standard curves, in serum, cover the range of 0.015 to 1 mlU/L for early pregnancy monitoring (7 to 15 weeks, referred to as Procedure I), and 0.531 to 17 mIU/L for mid and late pre...

journal_title：Clinical biochemistry

authors： Englebienne P,Doyen G,Dervaux R,Billemont JP

更新日期：1982-04-01 00:00:00

abstract：BACKGROUND:Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation of available methods mandatory. AIMS AND METHODS:We evaluated some preanalytical and analytical aspects of 25-OHD determi...

journal_title：Clinical biochemistry

authors： Bianchi S,Maffei S,Prontera C,Battaglia D,Vassalle C

更新日期：2012-12-01 00:00:00

abstract：:Collagen, the most abundant protein of the mammalian body, is specifically degraded by collagenase. Collagenase activity and subsequent collagen degradation are the main aspects of essential biological processes such as bone remodeling, tissue repair and wound healing. Measurement of collagenase activity is of interes...

journal_title：Clinical biochemistry

authors： Chakraborty A,Eghbali M

更新日期：1991-12-01 00:00:00

abstract：OBJECTIVE:Develop a simple LC-MS-MS method for determination of 25-hydroxymetabolites of vitamin D2 and D3 in serum. DESIGN AND METHODS:0.1 mL serum was mixed with 0.3 mL deproteinizing mixture containing two deuterated internal standards. Two LC-MS-MS systems were used: Waters Premier with an ESI source and a Hypersi...

journal_title：Clinical biochemistry

authors： Bogusz MJ,Al Enazi E,Tahtamoni M,Jawaad JA,Al Tufail M

更新日期：2011-11-01 00:00:00

abstract：OBJECTIVES:We investigated the relationships of biomarkers of various pathophysiologic pathways including high-sensitivity C-reactive protein (hs-CRP), lipocalin-2 (LCN2), myeloperoxidase (MPO) and matrix metalloproteinases 9 (MMP9) with mortality in stroke patients. DESIGN AND METHODS:hs-CRP, LCN2 and MPO concentrati...

journal_title：Clinical biochemistry

authors： Chan CP,Jiang HL,Leung LY,Wan WM,Cheng NM,Ip WS,Cheung KY,Chan RW,Wong LK,Graham CA,Renneberg R,Rainer TH

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVES:Therapeutic monitoring of everolimus with chromatographic methods (HPLC) enabled effective immunosuppression while limiting the incidence of drug-related adverse events. A fluorescence polarization immunoassay (FPIA) has been recently developed for the assessment of everolimus levels. The present study was d...

journal_title：Clinical biochemistry

authors： Baldelli S,Crippa A,Gabrieli R,Fiocchi R,Perico N,Merlini S,Ottomano C,Cattaneo D

更新日期：2006-12-01 00:00:00

abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...

journal_title：Clinical biochemistry

authors： Holla LI,Kankova K,Vasku A

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:It is unclear if the point-of-care (POC) Clinitest hCG device is subject to high-dose hook interference from physiological concentrations of intact human chorionic gonadotropin (hCG), β-core fragment of hCG (hCGβcf), and hCG free β-subunit (hCGβ) found in urine during pregnancy. We used a simulation model to...

journal_title：Clinical biochemistry

authors： Milhorn D,Korpi-Steiner N

更新日期：2015-02-01 00:00:00

abstract：:Increasing interest in the use of tumor markers in the clinical management of cancer patients has encouraged development of guidelines by local, national and international groups. Such guidelines generally include recommendations about which markers are likely to be most helpful in given circumstances. Particular requ...

journal_title：Clinical biochemistry

authors： Sturgeon CM

更新日期：2001-07-01 00:00:00

abstract：:The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP m...

journal_title：Clinical biochemistry

authors： Krahn J

更新日期：1983-04-01 00:00:00

abstract：OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...

journal_title：Clinical biochemistry

authors： Cui L,Lou Y,Zhang X,Zhou H,Deng H,Song H,Yu X,Xiao B,Wang W,Guo J

更新日期：2011-09-01 00:00:00

abstract：:Ethanolamine is a compound that is frequently seen in urinary amino acid analysis. Although there is a single report of increased ethanolamine excretion associated with a distinctive storage disease in two siblings, the significance of ethanolaminuria is not known. We measured urinary ethanolamine/creatinine ratios in...

journal_title：Clinical biochemistry

authors： Cole DE,Farag S,Dooley KC

更新日期：1988-10-01 00:00:00

abstract：:57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range o...

journal_title：Clinical biochemistry

authors： Carter P

更新日期：1978-06-01 00:00:00

abstract：:Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoc...

journal_title：Clinical biochemistry

authors： Quesniaux VF

更新日期：1991-02-01 00:00:00

abstract：:During sequential physiologic maturation of an individual follicle, the number of granulosa cells increases in excess of 1000-fold, while intra-ovarian concentrations of sex steroids escalate by 100-fold. The recent development of several in vitro ovarian systems has permitted a more extensive and direct assessment of...

journal_title：Clinical biochemistry

authors： Veldhuis JD

更新日期：1981-10-01 00:00:00

abstract：OBJECTIVES:Δ(9)-Tetrahydrocannabinol (THC) can be measured in exhaled breath by using an aerosol particle collection device. The sampling procedure is simple, non-invasive and takes only 2-3min. In the present study we measured the amount of THC in exhaled breath of cannabis users at specific time intervals up to 3h af...

journal_title：Clinical biochemistry

authors： Coucke L,Massarini E,Ostijn Z,Beck O,Verstraete AG

更新日期：2016-09-01 00:00:00

abstract：:The structure of enzyme active sites and the nature of the catalytic process are reviewed. The impressive efficiency of these protein catalysts appears to stem from such factors as proximity and orientation of enzyme and substrate moieties, strain, and the occurrence of distinctive microenvironments within catalytic c...

journal_title：Clinical biochemistry

authors： Blair AH

更新日期：1976-06-01 00:00:00

abstract：OBJECTIVES:Receptor for advanced glycation end products (RAGE) plays a role in inflammatory reactions. Soluble RAGE (sRAGE) level is elevated in patients with acute respiratory distress syndrome (ARDS). However, which clinical parameters and inflammatory biomarkers including sRAGE are associated with death in ARDS pati...

journal_title：Clinical biochemistry

authors： Nakamura T,Sato E,Fujiwara N,Kawagoe Y,Maeda S,Yamagishi S

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVES:To evaluate whether caspases are involved in ethanol (EtOH)-induced apoptosis and if polyenylphosphatidylcholine (PPC) affects apoptosis, in vitro in Hep G2 cells. METHODS:Cells were treated with 100 mmol/L EtOH for 24 h and with 2 doses of 100 mmol/L EtOH (1/24 h) in the presence of absence of 20 mmol/L of...

journal_title：Clinical biochemistry

authors： Katz GG,Shear NH,Malkiewicz IM,Valentino K,Neuman MG

更新日期：2001-05-01 00:00:00

abstract：UNLABELLED:The study of factors potentially associated with sleep bruxism (SB) may contribute to a better understanding of its nature and etiology. OBJECTIVES:In this way, this cross-sectional study aimed to evaluate the association between SB and salivary cortisol levels, heart rate, and other parafunctional habits. ...

journal_title：Clinical biochemistry

authors： Castelo PM,Barbosa Tde S,Pereira LJ,Fonseca FL,Gavião MB

更新日期：2012-06-01 00:00:00

abstract：BACKGROUND:Given that both insufficient and excess maternal iodine have adverse consequences such as poor cognitive performance, delayed physical development and increased fetal and infant mortality, the determination of maternal iodine status is very important. In this study, we established and verified a method invol...

journal_title：Clinical biochemistry

authors： Zou Y,Wang D,Yu S,Cheng X,Xia L,Yin Y,Xie S,Cheng Q,Qiu L,Lian X

更新日期：2020-08-01 00:00:00