Vitamin K-dependent gamma-glutamylcarboxylase in Atlantic salmon (Salmo salar L.).

Abstract:

:Due to problems with bone deformities in farmed Atlantic salmon, there is a growing interest in the possible involvement of vitamin K in normal bone development, and sensitive biomarkers for evaluating vitamin K status are therefore needed. The vitamin K-dependent (VKD) enzyme gamma-glutamylcarboxylase (GGCX, EC 6.4.x.x) requires vitamin K as a cofactor for its post-translational modification of glutamic acid (Glu) residues to gamma-carboxyglutamic acid (Gla) residues in VKD proteins, and is required for their function in haemostasis and bone metabolism. The present study was designed to evaluate the enzyme assay for GGCX activity in isolated liver microsomes and its distribution in the tissues of Atlantic salmon. The effect of KH(2) and menadione on the GGCX activity in salmon liver was also compared. Results from the present study show a widespread tissue distribution and expression of GGCX in Atlantic salmon. The GGCX activity and ggcx expression in all bony tissues examined imply the presence of vitamin K, and suggest the involvement of vitamin K in bone metabolism of Atlantic salmon. We propose the GGCX assay as a sensitive marker for vitamin K status, and confirm that menadione does not work as a cofactor for GGCX in Atlantic salmon liver.

journal_name

Fish Physiol Biochem

authors

Krossøy C,Lock EJ,Ørnsrud R

doi

10.1007/s10695-009-9335-5

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

627-635

issue

3

eissn

0920-1742

issn

1573-5168

pii

10.1007/s10695-009-9335-5

journal_volume

36

pub_type

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