Development of Cre-loxP technology in zebrafish to study the regulation of fish reproduction.

Abstract:

:One cannot seek permission to market transgenic fish mainly because there is no field test or any basic research on technological developments for evaluating their biosafety. Infertility is a necessary adjunct to exploiting transgenic fish unless completely secure land-locked facilities are available. In this study, we report the generation of a Cre transgenic zebrafish line using a cytomegalovirus promoter. We also produced fish carrying the Bax1 and Bax2 plasmids; these genes were separated by two loxP sites under a zona pellucida C promoter or were driven by an anti-Müllerian hormone promoter. We inserted a red fluorescent protein gene between the two loxP sites. After obtaining transgenic lines with the two transgenic fish crossed with each other (Cre transgenic zebrafish x loxP transgenic zebrafish), the floxed DNA was found to be specifically eliminated from the female or male zebrafish, and apoptosis gene expressions caused ovarian and testicular growth cessation and degeneration. Overexpression of the Bax1 and Bax2 genes caused various expression levels of apoptosis-related genes. Accordingly, this transgenic zebrafish model system provides a method to produce infertile fish and may be useful for application to genetically modified fish.

journal_name

Fish Physiol Biochem

authors

Lin HJ,Lee SH,Wu JL,Duann YF,Chen JY

doi

10.1007/s10695-013-9806-6

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

1525-39

issue

6

eissn

0920-1742

issn

1573-5168

journal_volume

39

pub_type

杂志文章