Abstract:
RATIONALE:Plakophilin-2 (PKP2) is an essential component of the cardiac desmosome. Recent data show that it interacts with other molecules of the intercalated disc. Separate studies show preferential localization of the voltage-gated sodium channel (Na(V)1.5) to this region. OBJECTIVE:To establish the association of PKP2 with sodium channels and its role on action potential propagation. METHODS AND RESULTS:Biochemical, patch clamp, and optical mapping experiments demonstrate that PKP2 associates with Na(V)1.5, and that knockdown of PKP2 expression alters the properties of the sodium current, and the velocity of action potential propagation in cultured cardiomyocytes. CONCLUSIONS:These results emphasize the importance of intermolecular interactions between proteins relevant to mechanical junctions, and those involved in electric synchrony. Possible relevance to the pathogenesis of arrhythmogenic right ventricular cardiomyopathy is discussed.
journal_name
Circ Resjournal_title
Circulation researchauthors
Sato PY,Musa H,Coombs W,Guerrero-Serna G,Patiño GA,Taffet SM,Isom LL,Delmar Mdoi
10.1161/CIRCRESAHA.109.201418subject
Has Abstractpub_date
2009-09-11 00:00:00pages
523-6issue
6eissn
0009-7330issn
1524-4571pii
CIRCRESAHA.109.201418journal_volume
105pub_type
杂志文章abstract::A central role for leukocytes in neointimal hyperplasia after arterial injury is suspected. However, the relative importance of neutrophils and monocytes in balloon or stent-induced injury are not well understood, and mechanistic targeting of leukocyte recruitment or function is crude. We determined the temporal and s...
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