Abstract:
:Confocal imaging was used to study the influence of cytosolic ATP on the properties of spontaneous Ca(2+) sparks in permeabilized ventricular myocytes. Cells were perfused with mock intracellular solutions containing fluo 3. Reducing [ATP] to <0.5 mmol/L decreased the frequency but increased the amplitude of spontaneous Ca(2+) sparks. In the presence of 20 micromol/L ATP, the amplitude increased by 48.7+/-10.9%, and the frequency decreased by 77.07+/-3.8%, relative to control responses obtained at 5 mmol/L ATP. After exposure to a solution containing zero ATP, the frequency of Ca(2+) sparks decreased progressively and approached zero within 90 seconds. As ATP washed out of the cell, the sarcoplasmic reticulum (SR) Ca(2+) content increased, until reaching a maximum after 3 minutes. Subsequent introduction of adenylyl imidodiphosphate precipitated a burst of large-amplitude Ca(2+) sparks. This was accompanied by a rapid decrease in SR Ca(2+) content to 80% to 90% of the steady-state value obtained in the presence of 5 mmol/L ATP. Thereafter, the SR Ca(2+) content declined much more slowly over 5 to 10 minutes. The effects of ATP withdrawal on Ca(2+) sparks may reflect reduced occupancy of the adenine nucleotide site on the SR Ca(2+) channel. These effects may contribute to previously reported changes in SR function during myocardial ischemia and reperfusion, in which ATP depletion and Ca(2+) overload occur.
journal_name
Circ Resjournal_title
Circulation researchauthors
Yang Z,Steele DSdoi
10.1161/hh1801.096264subject
Has Abstractpub_date
2001-09-14 00:00:00pages
526-33issue
6eissn
0009-7330issn
1524-4571journal_volume
89pub_type
杂志文章abstract::It has been demonstrated by other investigators that endothelium-dependent vasodilators are more effective on arterial tissue than on venous tissue. The purpose of this study was to determine if this was due to a difference in the sensitivity of arterial and venous smooth muscle to the endothelial dilator (EDRF) or to...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.60.4.626
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journal_title:Circulation research
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doi:10.1161/01.res.70.4.743
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journal_title:Circulation research
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更新日期:1975-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2015-01-30 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1161/01.res.66.6.1720
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000107196.21335.2B
更新日期:2004-01-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.69.3.757
更新日期:1991-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.70.2.234
更新日期:1992-02-01 00:00:00
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更新日期:2000-10-27 00:00:00
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更新日期:1999-10-29 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.67.6.1474
更新日期:1990-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.118.314416
更新日期:2019-09-13 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.68.5.1302
更新日期:1991-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.82.7.803
更新日期:1998-04-20 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.48.4.498
更新日期:1981-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.50.1.86
更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.83.1.73
更新日期:1998-07-13 00:00:00
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doi:10.1161/01.res.76.6.1057
更新日期:1995-06-01 00:00:00
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doi:10.1161/01.res.84.1.9
更新日期:1999-01-08 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/hh1701.095642
更新日期:2001-08-31 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2001-08-17 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-05-22 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.65.4.1136
更新日期:1989-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.82.8.908
更新日期:1998-05-04 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.108.178335
更新日期:2009-02-13 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.5.869
更新日期:1995-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.304382
更新日期:2014-09-26 00:00:00