Abstract:
:Cardiac hypertrophy induced by thyrotoxic stress leads to an increase in the rate of force development, velocity of shortening, tension-dependent heat generation, and myosin ATPase activity. We did studies to see whether alterations in covalent phosphorylation of myofibrillar proteins correlate with these changes. The protein preparations were isolated from control and thyrotoxic hearts of male albino rabbits freeze-clamped in situ. We measured myofibrillar ATPase, and the covalent phosphate content of ventricular myosin 19,000 (mol wt) light chain (P-light chain) and troponin I (TnI). The myofibrillar ATPase activity was increased 2-fold in the thyrotoxic preparations with no change in the level of myofibrillar phosphorylation. The covalent phosphate content of TnI was 1.21 +/- 0.09 mol P/mol TnI in control hearts and 1.14 +/- 0.04 mol P/mol TnI in thyrotoxic hearts. The covalent phosphate content of the light chain fraction was 0.41 +/- 0.06 mol P/mol P-light chain in control hearts and 0.37 +/- 0.04 mol P/mol P-light chain in thyrotoxic hearts. The dependence of the normalized myofibrillar ATPase on free calcium concentration was the same in control and thyrotoxic preparations. Thus the mechanical, thermal, and biochemical changes found in hearts from thyrotoxic animals probably occur with no change in phosphorylation of TnI or myosin light chains.
journal_name
Circ Resjournal_title
Circulation researchauthors
Litten RZ 3rd,Martin BJ,Howe ER,Alpert NR,Solaro RJdoi
10.1161/01.res.48.4.498subject
Has Abstractpub_date
1981-04-01 00:00:00pages
498-501issue
4eissn
0009-7330issn
1524-4571journal_volume
48pub_type
杂志文章abstract::Two characteristic features of the rapid component of the cardiac delayed rectifier current (IKr) are prominent inward rectification and an unexpected reduction in activating current with decreased [K+]o. Similar features are observed with heterologous expression of HERG, the gene thought to encode the channel carryin...
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更新日期:1990-02-01 00:00:00
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更新日期:2014-05-09 00:00:00
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更新日期:2013-04-12 00:00:00
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更新日期:2011-01-07 00:00:00
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更新日期:2011-01-21 00:00:00
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更新日期:2003-12-12 00:00:00
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更新日期:2018-02-02 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2004-02-20 00:00:00
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doi:10.1161/01.res.69.2.406
更新日期:1991-08-01 00:00:00
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更新日期:1981-11-01 00:00:00
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更新日期:2006-08-04 00:00:00
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更新日期:1984-03-01 00:00:00
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更新日期:2010-10-15 00:00:00
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更新日期:2003-11-14 00:00:00
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更新日期:2021-01-22 00:00:00
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更新日期:2015-08-14 00:00:00
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更新日期:1977-11-01 00:00:00
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更新日期:2012-01-20 00:00:00
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更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-05-25 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.72.5.984
更新日期:1993-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00