Abstract:
:To evaluate internal tandem duplication (ITD) insertion sites and length as well as their clinical impact in younger adult patients with FLT3-ITD-positive acute myeloid leukemia (AML), sequencing after DNA-based amplification was performed in diagnostic samples from 241 FLT3-ITD-mutated patients. All patients were treated on 3 German-Austrian AML Study Group protocols. Thirty-four of the 241 patients had more than 1 ITD, leading to a total of 282 ITDs; the median ITD length was 48 nucleotides (range, 15-180 nucleotides). ITD integration sites were categorized according to functional regions of the FLT3 receptor: juxtamembrane domain (JMD), n = 148; JMD hinge region, n = 48; beta1-sheet of the tyrosine kinase domain-1 (TKD1), n = 73; remaining TKD1 region, n = 13. ITD length was strongly correlated with functional regions (P < .001). In multivariable analyses, ITD integration site in the beta1-sheet was identified as an unfavorable prognostic factor for achievement of a complete remission (odds ratio, 0.22; P = .01), relapse-free survival (hazard ratio, 1.86; P < .001), and overall survival (hazard ratio, 1.59; P = .008). ITD insertion site in the beta1-sheet appears to be an important unfavorable prognostic factor in young adult patients with FLT3-ITD-positive AML. The clinical trials described herein have been registered as follows: AML HD93 (already published in 2003), AML HD98A (NCT00146120; http://www.ClinicalTrials.gov), and AMLSG 07-04 (NCT00151242; http://www.ClinicalTrials.gov).
journal_name
Bloodjournal_title
Bloodauthors
Kayser S,Schlenk RF,Londono MC,Breitenbuecher F,Wittke K,Du J,Groner S,Späth D,Krauter J,Ganser A,Döhner H,Fischer T,Döhner K,German-Austrian AML Study Group (AMLSG).doi
10.1182/blood-2009-03-209999subject
Has Abstractpub_date
2009-09-17 00:00:00pages
2386-92issue
12eissn
0006-4971issn
1528-0020pii
blood-2009-03-209999journal_volume
114pub_type
临床试验,杂志文章,多中心研究相关文献
BLOOD文献大全abstract::Although recent advances have enabled hematopoietic stem cells (HSCs) to be enriched to near purity, more information about their characteristics will improve our understanding of their development and stage-related functions. Here, using microarray technology, we identified endothelial cell-selective adhesion molecul...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-07-167106
更新日期:2009-03-26 00:00:00
abstract::Human plasma fibrinogen is produced by liver parenchymal cells. Such molecules contain two classes of gamma-chains (gamma A, gamma'), which differ with respect to their COOH-terminal sequences. When fibrin is crosslinked in the presence of factor XIIIa and Ca2+, three types of gamma-dimer are formed (gamma A-gamma A; ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Combination cytokine therapy continues to be evaluated in an effort to stimulate multilineage hematopoietic reconstitution after bone marrow myelosuppression. This study evaluated the efficacy of combination therapy with the synthetic interleukin-3 receptor agonist, Synthokine-SC55494, and recombinant methionyl human ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-05-15 00:00:00
abstract::Mucopolysaccharidosis type I-Hurler syndrome (MPS-IH) is a lysosomal storage disease characterized by multisystem morbidity and death in early childhood. Although hematopoietic cell transplantation (HCT) has been performed in these patients for more than 30 years, large studies on the long-term outcome of patients wit...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2014-11-608075
更新日期:2015-03-26 00:00:00
abstract::Thrombohemorrhagic complications are a major cause of morbidity and mortality in patients with essential thrombocythemia (ET) and polycythemia vera (PV). The pathogenesis of these complications is not completely clarified. Several studies have described abnormalities of red blood cells and platelets in these patients....
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-12-15 00:00:00
abstract::The coagulation factors V (FV) and VIII (FVIII) are important at sites of vascular injury for the amplification of the clotting cascade. Natural variants of these factors frequently lead to severe bleeding disorders. To understand the mechanisms of activation of FVIII by thrombin, we used a bank of mutant thrombins to...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0843
更新日期:2002-10-15 00:00:00
abstract::The anti-CD20 monoclonal antibody (mAb) rituximab has revolutionized the treatment of B-cell malignancies. This unprecedented success has not only substantially changed the mindset of the clinical community about the ability of mAb to improve outcomes but has catalyzed the interest in the pharmaceutical industry to de...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-07-298356
更新日期:2011-03-17 00:00:00
abstract::The Gardos channel is a Ca(2+)-sensitive, intermediate conductance, potassium selective channel expressed in several tissues including erythrocytes and pancreas. In normal erythrocytes, it is involved in cell volume modification. Here, we report the identification of a dominantly inherited mutation in the Gardos chann...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-04-642496
更新日期:2015-09-10 00:00:00
abstract::Loss-of-function mutation in the heme oxygenase 1 (Hmox1) gene causes a rare and lethal disease in children, characterized by severe anemia and intravascular hemolysis, with damage to endothelia and kidneys. Previously, we found that macrophages engaged in recycling of red cells were depleted from the tissues of Hmox1...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-02-554162
更新日期:2014-08-28 00:00:00
abstract::Adhesive interactions between CD34+ hematopoietic progenitor cells (HPC) and bone marrow stroma are crucial for normal hematopoiesis, yet their molecular bases are still poorly elucidated. We have investigated whether cell surface proteoglycan CD44 can mediate adhesion of human CD34+ HPC to immobilized hyaluronan (HA)...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-03-15 00:00:00
abstract::In this issue of Blood, Neufeld and colleagues present promising results on the safety and efficacy of a novel iron chelator for the treatment of transfusional iron overload. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-02-406744
更新日期:2012-04-05 00:00:00
abstract::Myeloma plasma cells constitute 10% to 90% of the total bone marrow cell count in patients with multiple myeloma (MM). These cells express a variety of cell surface markers, such as HLA-ABC and HLA-DR, and surface antigens that are necessary for professional antigen-presenting cells, including adhesion and costimulato...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::We have investigated the hormonal responsiveness of K562 cells using a serum-substituted in vitro clonogenic assay. Dexamethasone inhibited colony formation by the K562 cells, and the inhibitory effect could be reversed by progesterone (10(-6) M). Fluoxymesterone caused a prominent enhancement of K562 colony growth, w...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Nitric oxide (NO), an important effector molecule involved in immune regulation and host defense, was shown to induce apoptosis in lymphoma cells. In the present report the NO donor glycerol trinitrate was found to induce apoptosis in Jurkat cells that are sensitive to CD95-mediated kill. In contrast, a CD95-resistant...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::All-trans-retinoic acid (ATRA) downregulates the expression of two cellular procoagulants, tissue factor (TF) and cancer procoagulant (CP), in human promyelocytic leukemia cells. To evaluate whether or not changes of the procoagulant activities (PCAs) may share mechanisms with the ATRA-induced cyto-differentiation pro...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Pure red cell aplasia (PRCA) is a rare complication in patients with chronic lymphocytic leukemia (CLL). It is characterized by reticulocytopenia and by an absence of red cell precursors in the bone marrow. Unlike autoimmune hemolytic anemia, which is characterized by an increased number of reticulocytes, positive Coo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.3.1092
更新日期:2002-02-01 00:00:00
abstract::Our previous study demonstrated that 2-methoxyestradiol (2ME2), an estrogen derivative, induces apoptosis in multiple myeloma (MM) cells; however, the related transcriptional events are unclear. In the present study, we used oligonucleotide microarrays to identify genes altered during 2ME2-induced apoptosis in MM cell...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3146
更新日期:2003-05-01 00:00:00
abstract::Despite the frequency of HIV-specific CD8 T cells, most HIV-infected patients do not control viral replication without antiviral drugs. Although CD8 T cells are important in containing acute HIV and simian immunodeficiency virus (SIV) infection, CD8 T-cell functions are compromised in chronic infection. To investigate...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-03-0791
更新日期:2003-01-01 00:00:00
abstract::Truncation of asparaginase treatment due to asparaginase related toxicities or silent inactivation (SI) is common and may increase relapse risk in acute lymphoblastic leukemia (ALL). We investigated relapse risk following suboptimal asparaginase exposure among 1401 children aged 1-17 years, diagnosed with ALL between ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020006583
更新日期:2020-11-04 00:00:00
abstract::Administration of cyclosporine A (CsA) after autologous stem cell transplantation elicits an autoimmune syndrome with pathology similar to graft-versus-host disease (GVHD). This syndrome, termed autologous GVHD, is associated with the appearance of autoreactive T cells directed at major histocompatibility class (MHC) ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.3.868
更新日期:2001-08-01 00:00:00
abstract::We report a novel chromosome translocation--t(2;5)(p23;q35) or its variant, t(2;5;13)(p23;q35;q12)--in 3 patients with peripheral T-cell lymphoma. All 3 were female children who had peripheral lymphadenopathy without organomegaly and underwent complete remission with or without chemotherapy. Their tumors were characte...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-02-15 00:00:00
abstract::The Leu-8 molecule, the human homologue of the murine MEL-14 peripheral lymph node homing receptor, is expressed on neutrophils in both species and may be important in localization of cells to sites of inflammation. Most circulating human neutrophils express the Leu-8 molecule, and activation of neutrophils with phorb...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Containers constructed of polyvinylchloride (PVC) are used for the storage of platelet concentrates (PC) for transfusion, At 22 degrees C, pH often falls to such low levels (pH is less that 6.0) that viability is lost. Far lesser degrees of pH fall are observed in bags constructed of polyethylene (PE). In this study, ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Allogeneic hematopoietic stem-cell transplantation (HSCT) remains an effective strategy for inducing durable remission in chronic myeloid leukemia (CML). Reduced-intensity conditioning (RIC) regimens extend HSCT to older patients and those with comorbidities who would otherwise not be suitable candidates for HSCT. The...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-04-085969
更新日期:2007-11-01 00:00:00
abstract::The receptor for hyaluronic acid-mediated motility (RHAMM) is an antigen eliciting both humoral and cellular immune responses in patients with acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and multiple myeloma (MM). We initiated a phase 1 clinical trial vaccinating 10 patients with R3 (ILSLELMKL), a hi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-07-099366
更新日期:2008-02-01 00:00:00
abstract::The cell-surface expression of the MIC2 antigen defined by the monoclonal antibody 12E7 was investigated on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting. In contrast to preceding reports, we found that the MIC2 antigen is not restricted to...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-01-15 00:00:00
abstract::One hundred seventy-nine patients with acute nonlymphoblastic leukemia in first remission (n = 75), chronic myelocytic leukemia in chronic or accelerated phase (n = 48) or leukemia in advanced stage (n = 56) were given HLA-identical marrow grafts and randomized to receive methotrexate or cyclosporine for prevention of...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1988-02-01 00:00:00
abstract::We present four patients with adult T-cell leukemia (ATL) derived from a novel T-cell subset (CD4-, CD8- [double-negative, DN], T-cell receptor [TCR] alpha beta+). In the ATL cells of these patients, neither gene nor surface expression of CD4 and CD8 antigens was detected. Clinical and laboratory data showed no differ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-02-15 00:00:00
abstract::In previous studies we have found that monocytes produce soluble factors that stimulate human umbilical vein endothelial cells to produce granulocyte-macrophage colony-stimulating activity (CSA), burst-promoting activity (BPA), and megakaryocyte colony-stimulating activity (Meg-CSA) as well as factors that stimulate T...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Peripheral lymphocytes from normal individuals and from patients with chronic lymphocytic leukemia (CLL) were cultured in vitro for 1-7 days. The growth response to phytohemagglutinin (PHA) was quantitated by the incorporation of tritiated uridine into RNA nucleotide during a 2-hr pulse with the radioisotope. While th...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00