Abstract:
:The receptor for hyaluronic acid-mediated motility (RHAMM) is an antigen eliciting both humoral and cellular immune responses in patients with acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and multiple myeloma (MM). We initiated a phase 1 clinical trial vaccinating 10 patients with R3 (ILSLELMKL), a highly immunogenic CD8(+) T-cell epitope peptide derived from RHAMM. In 7 of 10 patients, we detected an increase of CD8(+)/HLA-A2/RHAMM R3 tetramer(+)/CD45RA(+)/CCR7(-)/CD27(-)/CD28(-) effector T cells in accordance with an increase of R3-specific CD8(+) T cells in enzyme linked immunospot (ELISpot) assays. In chromium release assays, a specific lysis of RHAMM-positive leukemic blasts was shown. Three of 6 patients with myeloid disorders (1/3 AML, 2/3 MDS) achieved clinical responses: one patient with AML and one with MDS showed a significant reduction of blasts in the bone marrow after the last vaccination. One patient with MDS no longer needed erythrocyte transfusions after 4 vaccinations. Two of 4 patients with MM showed a reduction of free light chain serum levels. Taken together, RHAMM-R3 peptide vaccination induced both immunologic and clinical responses, and therefore RHAMM constitutes a promising target for further immunotherapeutic approaches. This study is registered at http://ISRCTN.org as ISRCTN32763606 and is registered with EudraCT as 2005-001706-37.
journal_name
Bloodjournal_title
Bloodauthors
Schmitt M,Schmitt A,Rojewski MT,Chen J,Giannopoulos K,Fei F,Yu Y,Götz M,Heyduk M,Ritter G,Speiser DE,Gnjatic S,Guillaume P,Ringhoffer M,Schlenk RF,Liebisch P,Bunjes D,Shiku H,Dohner H,Greiner Jdoi
10.1182/blood-2007-07-099366subject
Has Abstractpub_date
2008-02-01 00:00:00pages
1357-65issue
3eissn
0006-4971issn
1528-0020pii
blood-2007-07-099366journal_volume
111pub_type
杂志文章相关文献
BLOOD文献大全abstract::Prolylcarboxypeptidase (PRCP) activates prekallikrein to plasma kallikrein, leading to bradykinin liberation, and degrades angiotensin II. We now identify PRCP as a regulator of blood vessel homeostasis. β-Galactosidase staining in PRCP(gt/gt) mice reveals expression in kidney and vasculature. Invasive telemetric moni...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-318527
更新日期:2011-04-07 00:00:00
abstract::The molecular determinants regulating the specification of human embryonic stem cells (hESCs) into hematopoietic cells remain elusive. HOXA9 plays a relevant role in leukemogenesis and hematopoiesis. It is highly expressed in hematopoietic stem and progenitor cells (HSPCs) and is downregulated upon differentiation. Ho...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-03-558825
更新日期:2014-11-13 00:00:00
abstract::In this issue of Blood, Goussetis et al identify autophagy as a new pathway for the degradation of the oncoprotein BCR-ABL. They show that the therapeutic drug arsenic trioxide (AS(2)O(3)) targets BCR-ABL for autophagic degradation via a p62/SQSTM1-dependent mechanism that is critical for the antileukemic effect of th...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2012-08-451492
更新日期:2012-10-25 00:00:00
abstract::Leukemic blasts from 774 children with newly diagnosed acute lymphocytic leukemia (ALL) have been phenotyped by microcytotoxicity testing with a panel of monoclonal antibodies and heteroantisera as part of a Pediatric Oncology Group classification study of acute leukemia. One hundred twenty-two cases, or 16% were desi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::Accumulating evidence has designated B cells as central players in the pathogenesis of immune diseases. In the late 1990s, anti-CD20 monoclonal antibodies were developed for the treatment of B-cell non-Hodgkin lymphomas, offering the opportunity to efficiently deplete the B-cell compartment for therapeutic immunointer...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2010-01-266668
更新日期:2010-07-29 00:00:00
abstract::Retrospective studies of patients with thrombotic microangiopathies (TMAs) have shown that a deficient activity of von Willebrand factor (vWF)-cleaving protease is involved in thrombotic thrombocytopenic purpura (TTP) but not in the hemolytic-uremic syndrome (HUS). To further analyze the relevance of this enzymatic ac...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood.v98.6.1765
更新日期:2001-09-15 00:00:00
abstract::We have previously shown that circulating progenitor cells in patients with polycythemia vera (PV) are hypersensitive to insulin-like growth factor I (IGF-I) with respect to erythroid burst formation in serum-free medium, and that this effect occurs through the IGF-I receptor. To investigate the molecular basis of thi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Although sickle cell disease (SCD) has a variable clinical course, many patients develop end-organ complications that are associated with significant morbidity and early mortality. Myeloablative allogeneic HSCT (allo-HSCT) is curative but has been historically performed only in children younger than 16 years of age. M...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2011-01-332510
更新日期:2011-08-04 00:00:00
abstract::Transferrin receptor 2 (TfR2) is a type 2 transmembrane protein expressed in hepatocytes that binds iron-bound transferrin (Tf). Mutations in TfR2 cause one form of hereditary hemochromatosis, a disease in which excessive absorption of dietary iron can lead to liver cirrhosis, diabetes, arthritis, and heart failure. T...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2477
更新日期:2004-12-15 00:00:00
abstract::miRNAs have emerged as master regulators of cancer-related events. miRNA dysregulation also occurs in Kaposi sarcoma (KS). Exploring the roles of KS-associated miRNAs should help to identify novel angiogenesis and lymphangiogenesis pathways. In the present study, we show that Kaposi sarcoma-associated herpesvirus (KSH...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-01-330589
更新日期:2011-09-08 00:00:00
abstract::Hairy cell leukemia variant (HCLv) presents with high disease burden, lack of typical antigens like CD25, and poor response to standard treatments like cladribine. Occasionally, patients with classic HCL respond poorly. Clinical and molecular features of HCL and HCLv has not been compared. Rearrangements expressing im...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-201731
更新日期:2009-11-19 00:00:00
abstract::In eighty-three patients with confirmed deep vein thrombosis, the fibrinolytic system was studied before and after a 10-minute venous occlusion. Blood was collected at least 3 months after the last acute episode, and PAI-1 antigen and activity, as well as tissue-type plasminogen activator (t-PA) antigen, urokinase-typ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Varying, limiting numbers of unseparated or purified cells (Ly-5.1), either from 14.5-day-old fetal liver (FL) or from adult bone marrow (BM) were coinjected with 10(5) unseparated BM cells (Ly-5.2) into lethally irradiated adult C57B1/6 recipients (Ly-5.2). The kinetics of donor cell repopulation of the lymphoid and ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-04-15 00:00:00
abstract::Tumor necrosis factor (TNF)-related activation-induced cytokine (TRANCE) induces osteoclast formation from monocyte/macrophage lineage cells via various transcription factors, including the Mi transcription factor (Mitf). Here, we show that inhibitors of differentiation/DNA binding (Ids), helix-loop-helix (HLH) transc...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-07-2798
更新日期:2006-04-01 00:00:00
abstract::We examined autonomous and interleukin-2 (IL-2)-responsive growth activities of leukemic cells derived from peripheral blood, as well as several clinical manifestations, including serum lactate dehydrogenase (LDH) level, of 35 patients with adult T-cell leukemia (ATL) to determine whether these properties were related...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::Stable gene replacement by in vivo administration of lentiviral vectors (LVs) has therapeutic potential for metabolic disorders and other systemic diseases. We studied the expression of intracellular and secreted proteins by LVs in immunocompetent mice. Liver, spleen, and bone marrow cells were efficiently transduced....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-09-3217
更新日期:2004-05-15 00:00:00
abstract::In an international collaborative study, a central histologic review identified 891 patients with essential thrombocythemia, strictly defined by World Health Organization criteria. After a median follow-up of 6.2 years, 109 (12%) patients experienced arterial (n = 79) or venous (n = 37) thrombosis. In multivariable an...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2011-02-339002
更新日期:2011-06-02 00:00:00
abstract::Langerhans cell histiocytosis (LCH) is a myeloproliferative disorder characterized by lesions composed of pathological CD207(+) dendritic cells with an inflammatory infiltrate. BRAFV600E remains the only recurrent mutation reported in LCH. In order to evaluate the spectrum of somatic mutations in LCH, whole exome sequ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-05-577825
更新日期:2014-11-06 00:00:00
abstract::Although small deletions, splice site abnormalities, missense, and nonsense mutations have been identified in patients with factor VII deficiency, there have been no reports of mutations in the factor VII promoter. We investigated a girl with factor VII levels that were less than 1% of normal in association with a sev...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Survivin and XIAP, members of the protein family known as the inhibitors of apoptosis, interfere with the activation of caspases, called the "cell death executioners." We examined Survivin (n = 116) and XIAP (n = 172) expression in primary acute myeloid leukemia (AML) blasts and assessed the impact of their expression...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-03-0960
更新日期:2003-12-01 00:00:00
abstract::Magnetic resonance imaging (MRI) is able to detect the increase of adipocytes in the hematopoietic bone marrow that occurs as a consequence of radiotherapy and is indicative of the loss of myeloid tissue. By monitoring this process, it is also possible to determine the recovery of the bone marrow. The amount of viable...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Macrophages are motile leukocytes, targeted by HIV-1, thought to play a critical role in host dissemination of the virus. However, whether infection impacts their migration capacity remains unknown. We show that 2-dimensional migration and the 3-dimensional (3D) amoeboid migration mode of HIV-1-infected human monocyte...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-08-596775
更新日期:2015-03-05 00:00:00
abstract::Twenty-two molecular diagnostic laboratories from 14 countries participated in a consortium study to estimate the impact of Factor VIII gene inversions in severe hemophilia A. A total of 2,093 patients with severe hemophilia A were studied; of those, 740 (35%) had a type 1 (distal) factor VIII inversion, and 140 (7%) ...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:
更新日期:1995-09-15 00:00:00
abstract::We developed a risk score to predict event-free survival (EFS) after allogeneic hematopoietic cell transplantation for sickle cell disease. The study population (n = 1425) was randomly split into training (n = 1070) and validation (n = 355) cohorts. Risk factors were identified and validated via Cox regression models....
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020005687
更新日期:2020-07-30 00:00:00
abstract::To determine whether the sensitivity of the human long-term culture-initiating cell (LTC-IC) assay could be increased, we have evaluated a spectrum of different fibroblast cell lines for their abilities to influence the number of cells detectable as LTC-IC, to influence LTC-IC maintenance, and/or to influence LTC-IC d...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-11-15 00:00:00
abstract::Using a polyclonal antiserum against canine CD34, we previously found that CD34 is expressed on canine bone marrow progenitor cells in a manner analogous to that found in humans. To further characterize CD34+ cells and to facilitate preclinical canine stem cell transplant studies, monoclonal antibodies (MoAbs) were ra...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-03-15 00:00:00
abstract::The interaction of thrombin with plasminogen activator inhibitor 1 (PAI-1) is shown to result in the simultaneous formation of both cleaved PAI-1 and a sodium dodecyl sulfate-stable thrombin-PAI-1 complex. The kinetics of this reaction can be described by a "suicide substrate" mechanism that includes a branched reacti...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Current monoclonal antibody therapies for multiple myeloma have had limited success, perhaps due to narrow target specificity. We have previously described the ability of polyclonal rabbit antithymocyte globulin (rATG) to induce caspase- and cathepsin-mediated apoptosis in human B and plasma cells. We now extend this ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2269
更新日期:2006-04-01 00:00:00
abstract::It has recently been shown that the ultralarge platelet-recruiting von Willebrand factor (VWF) strings formed immediately at exocytosis from endothelial cells may be anchored to the cell surface by interaction with the integral membrane protein P-selectin. This finding of a new binding partner for VWF immediately prom...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-09-3635
更新日期:2006-05-15 00:00:00
abstract::Macrophages and dendritic cells play an important role in regulating B-cell responses, including proliferation to antigens such as trinitrophenyl (TNP)-Ficoll and TNP-Brucella abortus. However, the mechanisms and molecule(s) that regulate these processes are relatively undefined. In this report, we show that human mac...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3123
更新日期:2003-06-01 00:00:00