Characterization of monoclonal antibodies that recognize canine CD34.

Abstract:

:Using a polyclonal antiserum against canine CD34, we previously found that CD34 is expressed on canine bone marrow progenitor cells in a manner analogous to that found in humans. To further characterize CD34+ cells and to facilitate preclinical canine stem cell transplant studies, monoclonal antibodies (MoAbs) were raised to CD34. A panel of 10 MoAbs was generated that reacted with recombinant CD34 and with CD34+ cell lines and failed to react with CD34- cell lines. Binding properties of five purified MoAbs were determined by BIAcore analysis and flow cytometric staining, and several MoAbs showed high affinity for CD34. Two antibodies, 1H6 and 2E9, were further characterized, and in flow cytometry studies typically 1% to 3% of stained bone marrow cells were CD34+. Purified CD34+ bone marrow cells were 1.8- to 55-fold enriched for colony-forming unit-granulocyte-macrophage and for long-term culture initiating cells as compared with bone marrow mononuclear cells, whereas CD34- cells were depleted of progenitors. Three autologous transplants were performed with CD34+ cell fractions enriched by immunomagnetic separation. After marrow ablative total body irradiation (920 cGy), prompt hematopoietic recovery was seen with transplanted cell doses of

journal_name

Blood

journal_title

Blood

authors

McSweeney PA,Rouleau KA,Wallace PM,Bruno B,Andrews RG,Krizanac-Bengez L,Sandmaier BM,Storb R,Wayner E,Nash RA

subject

Has Abstract

pub_date

1998-03-15 00:00:00

pages

1977-86

issue

6

eissn

0006-4971

issn

1528-0020

journal_volume

91

pub_type

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