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A review of experimental techniques used for the heterologous expression of nicotinic acetylcholine receptors.

Abstract：

:Nicotinic acetylcholine receptors (nAChRs) are members of the Cys-loop family of neurotransmitter-gated ion channels, a family that also includes receptors for gamma-aminobutyric acid, glycine and 5-hydroxytryptamine. In humans, nAChRs have been implicated in several neurological and psychiatric disorders and are major targets for pharmaceutical drug discovery. In addition, nAChRs are important targets for neuroactive pesticides in insects and in other invertebrates. Historically, nAChRs have been one of the most intensively studied families of neurotransmitter receptors. They were the first neurotransmitter receptors to be biochemically purified and the first to be characterized by molecular cloning and heterologous expression. Although much has been learnt from studies of native nAChRs, the expression of recombinant nAChRs has provided dramatic advances in the characterization of these important receptors. This review will provide a brief history of the characterization of nAChRs by heterologous expression. It will focus, in particular, upon studies of recombinant nAChRs, work that has been conducted by many hundreds of scientists during a period of almost 30 years since the molecular cloning of nAChR subunits in the early 1980s.

journal_name

Biochem Pharmacol

journal_title

Biochemical pharmacology

authors

Millar NS

doi

10.1016/j.bcp.2009.06.015

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

766-76

issue

7

eissn

0006-2952

issn

1873-2968

pii

S0006-2952(09)00482-1

journal_volume

78

pub_type

杂志文章,评审

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