Abstract:
:The effects of thiamine thiazolone (TT) and thiamine thiazolone pyrophosphate (TTPP) on the in vitro and in vivo inhibition of pyruvate dehydrogenase complex (PDHC) from rat cortex and hippocampus were characterized. TTPP decreased PDHC activity in vitro but had no effect in vivo following its direct chronic administration via osmotic mini-pumps into the brains of behaving rats. In contrast, TT had no direct effect in vitro following a differential centrifugation purification of the mitochondrial PDHC fraction, but decreased PDHC activity in vivo. Additional experiments demonstrated that the cytosolic fraction converted TT to TTPP which, in turn, inhibited PDHC in vitro. A mechanism is proposed to explain these effects that is consistent with a non-competitive inhibition of brain PDHC by TTPP.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Kandiko CT,Smith D,Yamamoto BKdoi
10.1016/0006-2952(88)90620-xsubject
Has Abstractpub_date
1988-11-15 00:00:00pages
4375-80issue
22eissn
0006-2952issn
1873-2968pii
0006-2952(88)90620-Xjournal_volume
37pub_type
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