Abstract：
:The human platelet thromboxane A2/prostaglandin H2 receptor has been purified 6100-fold to apparent homogeneity by a three-step chromatographic procedure with an overall yield of 6%. A 6-fold purification of the receptor was first achieved by chromatography of 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate (CHAPS)-solubilized membrane proteins from human platelets on a diethylaminoethyl (DEAE)-Sepharose column. The DEAE eluate fractions containing receptor activity were then applied to a newly developed affinity column using the cyclohexyl derivative of SQ30,741 (SQ31,491) as the immobilized ligand. Elution of the receptor from the affinity column with BM13.177 yielded a further purification of 1700-fold. An additional 4-fold receptor purification from the affinity column eluate was achieved by HPLC using GPC 500 and GPC 100 columns connected in tandem. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining of the HPLC eluate containing purified receptor revealed a single, distinct band with a molecular weight of 55,000. The receptor binding activity was detected with [3H]SQ29,548 using a newly developed binding assay which involved immobilization of the receptor on polyethyleneimine-treated glass fiber filters. The binding of [3H]SQ29,548 to the purified receptor was time dependent, saturable, reversible and highly specific. Unlabeled SQ29,548, BM13.505, and U46619 (but not thromboxane B2 or 6-keto prostaglandin F1 alpha) competed for [3H]SQ29,548 binding to the purified receptor in a concentration-dependent manner. Scatchard analysis of [3H]SQ29,548 binding to the purified receptor revealed the presence of a single class of high-affinity binding sites, with a Kd of 4 nM and a Bmax of 17 nmol/mg protein.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Kim SO,Lim CT,Lam SC,Hall SE,Komiotis D,Venton DL,Le Breton GCdoi
10.1016/0006-2952(92)90294-ssubject
Has Abstractpub_date
1992-01-22 00:00:00pages
313-22issue
2eissn
0006-2952issn
1873-2968pii
0006-2952(92)90294-Sjournal_volume
43pub_type
杂志文章abstract：:During attempts to isolate and identify an endogenous ligand for the glycoside binding sites on Na+,K+-ATPase, bovine adrenal glands were found to contain a potent inhibitor of isolated Na+,K+-ATPase. The inhibitory principle was extracted from adrenal cortex, following homogenization in NaHCO3 solution and separation...
journal_title：Biochemical pharmacology
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journal_title：Biochemical pharmacology
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doi：10.1016/0006-2952(85)90613-6
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journal_title：Biochemical pharmacology
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doi：10.1016/0006-2952(88)90062-7
更新日期：1988-10-15 00:00:00
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doi：10.1016/0006-2952(90)90516-n
更新日期：1990-05-15 00:00:00
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doi：10.1016/0006-2952(86)90663-5
更新日期：1986-11-01 00:00:00
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doi：10.1016/s0006-2952(02)01453-3
更新日期：2003-01-01 00:00:00
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doi：10.1016/0006-2952(82)90341-0
更新日期：1982-03-15 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：2010-12-15 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(95)00155-s
更新日期：1995-08-08 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(83)90196-x
更新日期：1983-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0006-2952(87)90222-x
更新日期：1987-09-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(83)90047-3
更新日期：1983-06-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(88)90094-9
更新日期：1988-11-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(92)90254-g
更新日期：1992-02-18 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(82)90260-x
更新日期：1982-09-15 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1993-03-09 00:00:00
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更新日期：2002-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：1983-05-01 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
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更新日期：1986-02-15 00:00:00
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更新日期：2012-12-15 00:00:00
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pub_type： 杂志文章
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更新日期：2007-09-15 00:00:00
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pub_type： 杂志文章
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更新日期：2014-01-15 00:00:00
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pub_type： 杂志文章
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更新日期：2002-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-10-18 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(94)90575-4
更新日期：1994-10-07 00:00:00
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journal_title：Biochemical pharmacology
pub_type： 杂志文章
doi：10.1016/0006-2952(85)90145-5
更新日期：1985-09-01 00:00:00
