Abstract:
:In this study we analyse the effects of the anti-tumor compound distamycin on the binding of nuclear factor(s) to a synthetic oligonucleotide (GTATA/IFN-gamma) mimicking a putative regulatory region of the human HLA-DR alpha gene. This region contains the sequence (GTATA), that is required for nuclear protein binding and is likely to interact with distamycin. The present results, by showing that distamycin inhibits the interaction between nuclear factors and the GTATA/IFN-gamma oligonucleotide, suggest that distamycin might alter the binding of transacting factors to cis-elements containing AT/TA sequences. Alterations of nuclear protein binding to specific target sequences could be one of the molecular mechanism(s) by which distamycin exerts its antiproliferative activity on living cells.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Gambari R,Barbieri R,Nastruzzi C,Chiorboli V,Feriotto G,Natali PG,Giacomini P,Arcamone Fdoi
10.1016/0006-2952(91)90620-ksubject
Has Abstractpub_date
1991-02-15 00:00:00pages
497-502issue
4eissn
0006-2952issn
1873-2968pii
0006-2952(91)90620-Kjournal_volume
41pub_type
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