2,5-Hexanedione modifies skeletal proteins of the red blood cells and increases the binding of hemoglobin to the membrane.


:The effects of 2,5-hexanedione (2,5 HD) on skeletal proteins of red blood cells (RBCs) were investigated both in vitro (human RBCs) and in vivo in male Sprague-Dawley rats which had been treated with the drug for several days. We found that 2,5 HD induced the following major changes in the electrophoretic pattern of the skeletal proteins: (i) the appearance of high-molecular weight bands, (ii) a dose-dependent decrease in spectrin Bands 1 and 2, and (iii) a dose-dependent increase in the amount of hemoglobin (Hb) associated with the membrane. Membranoskeletons, prepared from resealed ghosts which had been previously treated with 2,5 HD, were able to bind an increased amount of Hb from untreated RBCs, thus suggesting a drug-induced modification of the membrane. Extraction of spectrin and actin from ghosts did not remove the membrane-bound Hb and, furthermore, Hb bound to 2,5 HD-treated membranes mainly bearing Band 3 and free of peripheral proteins. These data suggested a 2,5 HD-induced modification of an intrinsic membrane protein, probably Band 3. This hypothesis was consistent with the observation that 2,5 HD also induced a modification of Band 3 aminogroups, as evidenced by a dose-dependent decrease in the binding of eosin probes. Furthermore, RBCs treated in vitro with 2,5 HD bound an increased amount of autologous immunoglobulins (IgG). As reported by Kay and Low et al. the binding of autologous IgG is a phenomenon associated with the aging process of RBCs and may involve a modification of Band 3. Our data show that RBCs treated with 2,5 HD acquired various characteristics of senescent cells such as spectrin cross-linking, Hb-membrane binding and increased IgG binding, and suggest that 2,5 HD treatment might affect RBC survival.


Biochem Pharmacol


Biochemical pharmacology


Mallozzi C,Scorza G,Frontali N,Minetti M




Has Abstract


1989-08-15 00:00:00














  • Properties of hydrogen peroxide-induced histamine release from rat mast cells.

    abstract::Incubation of rat peritoneal mast cells with hydrogen peroxide results in a marked release of histamine. Maximal release is observed with 0.05-0.1 mM H(2)O(2), but higher concentrations of H(2)O(2) instead suppresses the release. Histamine release starts after about 2 min of lag time and reaches a plateau in about 10 ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ohmori H,Yamamoto I,Akagi M,Tasaka K

    更新日期:1980-03-01 00:00:00

  • Ca2+/calmodulin-dependent protein kinase II regulation by inhibitor 1 of protein phosphatase 1 alleviates necroptosis in high glucose-induced cardiomyocytes injury.

    abstract::Ca2+/calmodulin-dependent protein kinase II (CaMKII) plays an important role in the cardiovascular system. However, the potential protective role of inhibitor 1 of protein phosphatase 1 (I1PP1), which is able to regulate CaMKII, in high glucose-induced cardiomyocytes injury remains unknown. In the present study, cardi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Sun L,Chen Y,Luo H,Xu M,Meng G,Zhang W

    更新日期:2019-05-01 00:00:00

  • Attenuation by glutathione of hsp72 gene expression induced by cadmium in cisplatin-resistant human ovarian cancer cells.

    abstract::Intracellular GSH has some effects on protecting cells against cadmium and is involved in the development of resistance to cisplatin (CDDP). To determine the effects of intracellular GSH on expression of the heat shock genes (hsp) induced by cadmium in CDDP-resistant cancer cells, we used two human ovarian cancer cell...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Abe T,Gotoh S,Higashi K

    更新日期:1999-07-01 00:00:00

  • Antiplatelet activity of some prenylflavonoids.

    abstract::Eight naturally occurring prenylflavonoids were tested for their antiplatelet activities in rabbit platelet suspension. Cyclomorusin and artomunoxanthone showed strong inhibition of platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) induced platelet aggregation. Cyclomulberrin, dihydroiso...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Lin CN,Shieh WL,Ko FN,Teng CM

    更新日期:1993-01-26 00:00:00

  • Relative contribution of rat cytochrome P450 isoforms to the metabolism of caffeine: the pathway and concentration dependence.

    abstract::The aim of the present study was to estimate the relative contribution of rat P450 isoforms to the metabolism of caffeine and to assess the usefulness of caffeine as a marker substance for estimating the activity of P450 in rat liver and its potential for pharmacokinetic interactions in pharmacological experiments. Th...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kot M,Daniel WA

    更新日期:2008-04-01 00:00:00

  • Selective inhibition of cyclic AMP and cyclic GMP phosphodiesterases of cardiac nuclear fraction.

    abstract::Approximately 60% of the total particulate phosphodiesterase activity occurring in cardiac tissue was associated with the nuclear fraction. Cyclic GMP phosphodiesterase activity of the purified cardiac nuclear fraction was selectively inhibited by trifluoperazine (I50 = 19 microM) with negligible inhibition (less than...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ahluwalia GS,Rhoads AR

    更新日期:1982-03-01 00:00:00

  • Effect of pretreatment with sodium phenobarbital on the toxicity of soman in mice.

    abstract::Pretreatment with sodium phenobarbital induces hepatic microsomal enzymes which are responsible for the metabolic breakdown of a large number of endogenous and exogenous chemical compounds. A previous study [K. P. DuBois and F. K. Kinoshita, Proc. Soc. exp. Biol. Med. 129, 699 (1968)] reported that phenobarbital pretr...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Clement JG

    更新日期:1983-04-15 00:00:00

  • Effect of diethyl maleate on the biliary excretion rate of infused sulfobromophthalein-glutathione.

    abstract::Diethyl maleate (DEM) was given intraperitoneally to rats in a dose (4.3 mmoles/kg) known to markedly decrease glutathione levels in liver. DEM induced a choleresis previously shown to be due to the osmotic activity of DEM conjugates (DEM-glutathione and subsequent metabolic products) excreted into bile. Coincident wi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Combes B,Backof B

    更新日期:1982-08-15 00:00:00

  • Configurational requirements of the sugar moiety for the pharmacological activity of anthracycline disaccharides.

    abstract::The amino sugar is recognized to be a critical determinant of the activity of anthracycline monosaccharides related to doxorubicin and daunorubicin. In an attempt to improve the pharmacological properties of such agents, novel anthracycline disaccharides have been designed in which the amino sugar, daunosamine, is sep...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Arcamone F,Animati F,Bigioni M,Capranico G,Caserini C,Cipollone A,De Cesare M,Ettorre A,Guano F,Manzini S,Monteagudo E,Pratesi G,Salvatore C,Supino R,Zunino F

    更新日期:1999-05-15 00:00:00

  • Biotransformation of 17 beta-hydroxy-11 beta-(4-dimethylaminophenyl)17 alpha-1-propynyl-estra-4,9-dien-3-one (RU486) in rat hepatoma variants.

    abstract::Metabolism of the synthetic steroid 17 beta-hydroxy-11 beta-(4-dimethylaminophenyl)17 alpha-1-propynyl-estra-4,9-dien-3-one (RU486) occurs in the dedifferentiated S-H56-125 variant of Reuber hepatoma. Considering that rat liver cytochrome P450 (P450) monooxygenases are engaged in different oxidative steps of the metab...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Chasserot-Golaz S,Beck G,Venetianer A

    更新日期:1993-12-03 00:00:00

  • Stimulation of mucus glycoprotein biosynthesis in rat gastric mucosa by gastrin.

    abstract::We examined the effects of the gastrin family of peptides on gastric mucus glycoprotein (mucin) biosynthesis in rat gastric mucosa using an organ culture technique. Radiolabeled mucin was obtained from the tissue and culture medium of the corpus and antrum of rat stomach incubated for 5 hr with [3H]glucosamine (GlcN),...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ichikawa T,Ishihara K,Saigenji K,Hotta K

    更新日期:1993-11-02 00:00:00

  • N-butyldeoxygalactonojirimycin: a more selective inhibitor of glycosphingolipid biosynthesis than N-butyldeoxynojirimycin, in vitro and in vivo.

    abstract::N-Butyldeoxynojirimycin (NB-DNJ) inhibits the ceramide glucosyltransferase which catalyses the first step in glycosphingolipid (GSL) biosynthesis. It has the potential to be used for the treatment of the GSL lysosomal storage diseases and is currently in clinical trials for the treatment of type 1 Gaucher's disease. H...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Andersson U,Butters TD,Dwek RA,Platt FM

    更新日期:2000-04-01 00:00:00

  • Evaluation of in vitro PXR-based assays and in silico modeling approaches for understanding the binding of a structurally diverse set of drugs to PXR.

    abstract::The pregnane X-receptor (PXR) is a promiscuous nuclear receptor primarily responsible for the induction of genes from the cytochrome P450 3A family. In this study, we used a previously described PXR/SRC tethered protein to establish two in vitro assays for identifying PXR ligands: automated ligand identification syste...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Xiao L,Nickbarg E,Wang W,Thomas A,Ziebell M,Prosise WW,Lesburg CA,Taremi SS,Gerlach VL,Le HV,Cheng KC

    更新日期:2011-03-01 00:00:00

  • Effects of sulfobromophthalein and ethacrynic acid on glyceryl trinitrate relaxation.

    abstract::The effects of sulfobromophthalein (SBP) and ethacrynic acid (ECA), both inhibitors of glutathione S-transferase (GST), or glyceryl trinitrate (GTN)-induced vasorelaxation were investigated in rabbit aortic strips. The aortic strips were pre-contracted with phenylephrine, followed by relaxation with 0.5 microM GTN, wi...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Lau DT,Benet LZ

    更新日期:1992-05-28 00:00:00

  • Activation of misonidazole by rat liver microsomes and purified NADPH-cytochrome c reductase.

    abstract::Rat liver microsomes and purified NADPH-cytochrome c reductase metabolized [14C]misonidazole anaerobically to a reactive intermediate that covalently binds to tissue macromolecules. Air strongly inhibited the binding whereas carbon monoxide had no effect, indicating that misonidazole is activated via reduction and not...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: McManus ME,Lang MA,Stuart K,Strong J

    更新日期:1982-02-15 00:00:00

  • Dexamethasone and etoposide induce apoptosis in rat thymocytes from different phases of the cell cycle.

    abstract::Dexamethasone and etoposide both induce apoptosis in immature rat thymocytes. We investigated the dependence of apoptosis on the phase of the cell cycle after incubation with these drugs. Cell cycle progression was followed by a combination of pulse labelling with 5-bromo-2'-deoxyuridine (BrdU), labelling fixed cells ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Fearnhead HO,Chwalinski M,Snowden RT,Ormerod MG,Cohen GM

    更新日期:1994-09-15 00:00:00

  • Chiral inversion of drug: role of intestinal bacteria in the stereoselective sulphoxide reduction of flosequinan.

    abstract::Chiral inversion at a sulphoxide position of flosequinan enantiomers [(+/-)-7-fluoro-1-methyl-3-methylsulphinyl-4-quinolone] occurred in conventional rats but not in either germ-free rats or rats treated with antibiotics after an oral administration of each enantiomer. Thus, it was postulated that the chiral inversion...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kashiyama E,Yokoi T,Todaka T,Odomi M,Kamataki T

    更新日期:1994-07-19 00:00:00

  • Protective effect of interleukin-6 against the death of PC12 cells caused by serum deprivation or by the addition of a calcium ionophore.

    abstract::Interleukin-6 (IL-6) is known to differentiate the rat pheochromocytoma cell line PC12 to neuron-like cells. We examined the effect of IL-6 on the death of PC12 cells. IL-6 significantly blocked the death of PC12 cells by serum deprivation. The protective effect of IL-6 was increased by preincubation of PC12 with IL-6...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Umegaki H,Yamada K,Naito M,Kameyama T,Iguchi A,Nabeshima T

    更新日期:1996-09-27 00:00:00

  • Binding of [3H]quinuclidinyl benzilate to intestinal mucus. An artifact in identification of epithelial cell muscarinic receptors.

    abstract::The widely used muscarinic receptor ligand [3H]quinuclidinyl benzilate ([3H]QNB) was found to bind in a site-specific but artifactual manner to rat intestinal mucus, obscuring specific binding to muscarinic receptors on intestinal epithelial cells. Atropine inhibited [3H]QNB binding to mucus with an apparent IC50 of 2...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Rimele TJ,Gaginella TS

    更新日期:1982-02-15 00:00:00

  • Explanation of the non-hyperbolic kinetics of the glutathione S-transferases by the simplest steady-state random sequential Bi Bi mechanism.

    abstract::We have demonstrated that the simplest steady-state random sequential Bi Bi mechanism is sufficient to explain the previously reported non-hyperbolic kinetics of glutathione S-transferase 3-3 [Pabst MJ et al., J Biol Chem 249: 7140-7150, 1974; Jakobson I et al., Biochem J 177: 861-868, 1979]. The metabolism of 1-chlor...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Ivanetich KM,Goold RD,Sikakana CN

    更新日期:1990-06-15 00:00:00

  • Impact of terminal dimethylation on the resistance profile of α-N-heterocyclic thiosemicarbazones.

    abstract::Triapine is an α-N-heterocyclic thiosemicarbazone with promising anticancer activity against hematologic malignancies but widely ineffective against solid tumor types in clinical trials. The anticancer activity of thiosemicarbazones can be dramatically increased by terminal dimethylation. KP1089 is a gallium compound ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Heffeter P,Pirker C,Kowol CR,Herrman G,Dornetshuber R,Miklos W,Jungwirth U,Koellensperger G,Keppler BK,Berger W

    更新日期:2012-06-15 00:00:00

  • Cationic environment and secretin secretion in canine duodenal mucosa in vitro.

    abstract::We examined the effects of the divalent cation calcium (Ca2+) and the monovalent cations potassium (K+) and sodium (Na+) and different modalities that affect the fluxes of these cations on immunoreactive secretin (IRS) secretion from canine duodenal mucosa in vitro. In the absence of extracellular Ca2+, the basal IRS ...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Murthy SN,Lavy A

    更新日期:1988-03-15 00:00:00

  • Curcumin induces electrical activity in rat pancreatic beta-cells by activating the volume-regulated anion channel.

    abstract::Curcumin, the principal active component of turmeric, is reported to exert a number of therapeutic actions, including a hypoglycaemic/antidiabetic action. The underlying mechanisms to this action are essentially unknown. We have investigated the hypothesis that a direct stimulatory action on the pancreatic beta-cell c...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Best L,Elliott AC,Brown PD

    更新日期:2007-06-01 00:00:00

  • Aspirin and indomethacin reduce lung inflammation of mice exposed to cigarette smoke.

    abstract::Neutrophil accumulation response to cigarette smoke (CS) in humans and animal models is believed to play an important role in pathogenesis of many tobacco-related lung diseases. Here we evaluated the lung anti-inflammatory effect of aspirin and indomethacin in mice exposed to CS. C57BL/6 mice were exposed to four ciga...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Castro P,Nasser H,Abrahão A,Dos Reis LC,Riça I,Valença SS,Rezende DC,Quintas LE,Cavalcante MC,Porto LC,Koatz VL

    更新日期:2009-03-15 00:00:00

  • Differential roles of cytochromes P450 2D1, 2C11, and 1A1/2 in the hydroxylation of bufuralol by rat liver microsomes.

    abstract::Bufuralol hydroxylation activities of liver microsomal cytochrome P450 (P450) enzymes were studied in the rat; the reaction has been used widely in determining levels of liver microsomal P450 2D6, which shows debrisoquine-type genetic polymorphism in humans. Liver microsomes catalyzed the conversion of bufuralol to 1'...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Mimura M,Yamazaki H,Sugahara C,Hiroi T,Funae Y,Shimada T

    更新日期:1994-06-01 00:00:00

  • On the specificity of verapamil as a calcium channel-blocker.

    abstract::The stimulated uptake of 45Ca2+ into incubated cerebrocortical synaptosomes caused by veratrine (75 microM) was blocked by low concentrations of verapamil (0.5-30 microM) which did not prevent or reduce depolarization as judged by efflux of potassium (K+). However, verapamil did not prevent amino acid neutrotransmitte...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Norris DK,Bradford HF

    更新日期:1985-06-01 00:00:00

  • Structure-activity relationship of pyrimidine base analogs as ligands of orotate phosphoribosyltransferase.

    abstract::Eighty pyrimidine base analogs were evaluated as inhibitors of mouse liver orotate phosphoribosyltransferase (OPRTase, EC Based on these findings and an extensive literature review, a structure-activity relationship has been formulated for the binding of pyrimidine base analogs to OPRTase. This study provid...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Niedzwicki JG,Iltzsch MH,el Kouni MH,Cha S

    更新日期:1984-08-01 00:00:00

  • Deoxyribosyl exchange reactions leading to the in vivo generation and regeneration of the antiviral agents (E)-5-(2-bromovinyl)-2'-deoxyuridine, 5-ethyl-2'-deoxyuridine and 5-(2-chloroethyl)-2'-deoxyuridine.

    abstract::In the rat, the highly potent anti-herpes drug (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdUrd) is rapidly converted to its base (E)-5-(2-bromovinyl)uracil (BVUra) through the action of pyrimidine nucleoside phosphorylases. However, BVdUrd can be regenerated or even generated de novo from BVUra by a pentosyl transfer rea...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Desgranges C,De Clercq E,Razaka G,Drouillet F,Belloc I,Bricaud H

    更新日期:1986-05-15 00:00:00

  • Drug metabolism in hepatocyte sandwich cultures of rats and humans.

    abstract::Adult hepatocytes from rat and man were maintained for 2 weeks between two gel layers in a sandwich configuration to study the influence of this culture technique on the preservation of basal activities of xenobiotic-metabolizing phase I and phase II enzymes. The response of these enzyme activities to an enzyme induce...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Kern A,Bader A,Pichlmayr R,Sewing KF

    更新日期:1997-10-01 00:00:00

  • Anti-fibrotic and anti-inflammatory properties of melatonin on human gingival fibroblasts in vitro.

    abstract::Melatonin (MEL) has been proposed as a therapeutic agent for the oral cavity, due to its antioxidant and anti-inflammatory effects since periodontal diseases are aggravated by free radicals, and by disproportionate immunological response to plaque microorganism. In addition, MEL promotes bone formation. This study aim...

    journal_title:Biochemical pharmacology

    pub_type: 杂志文章


    authors: Gómez-Florit M,Ramis JM,Monjo M

    更新日期:2013-12-15 00:00:00