Abstract:
:Endothelial progenitor cells are critically involved in essential biologic processes, such as vascular homeostasis, regeneration, and tumor angiogenesis. Endothelial colony-forming cells (ECFCs) are endothelial progenitor cells with robust proliferative potential. Their profound vessel-forming capacity makes them a promising tool for innovative experimental, diagnostic, and therapeutic strategies. Efficient and safe methods for their isolation and expansion are presently lacking. Based on the previously established efficacy of animal serum-free large-scale clinical-grade propagation of mesenchymal stromal cells, we hypothesized that endothelial lineage cells may also be propagated efficiently following a comparable strategy. Here we demonstrate that human ECFCs can be recovered directly from unmanipulated whole blood. A novel large-scale animal protein-free humanized expansion strategy preserves the progenitor hierarchy with sustained proliferation potential of more than 30 population doublings. By applying large-scale propagated ECFCs in various test systems, we observed vascular networks in vitro and perfused vessels in vivo. After large-scale expansion and cryopreservation phenotype, function, proliferation, and genomic stability were maintained. For the first time, proliferative, functional, and storable ECFCs propagated under humanized conditions can be explored in terms of their therapeutic applicability and risk profile.
journal_name
Bloodjournal_title
Bloodauthors
Reinisch A,Hofmann NA,Obenauf AC,Kashofer K,Rohde E,Schallmoser K,Flicker K,Lanzer G,Linkesch W,Speicher MR,Strunk Ddoi
10.1182/blood-2008-09-181362subject
Has Abstractpub_date
2009-06-25 00:00:00pages
6716-25issue
26eissn
0006-4971issn
1528-0020pii
blood-2008-09-181362journal_volume
113pub_type
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