Abstract:
:Hybridomas producing proinsulin antibodies were cloned by limiting dilution of cell cultures obtained by fusion of splenocytes of immunized mice with immortal myeloma cells. Some proinsulin monoclonal antibodies crossreacted with labelled insulin but none did with labelled C-peptide indicating that the involved epitopes were at one of the insulin/C-peptide junctions or included in the insulin moiety. Hybridoma supernatants were assayed for IgG concentration by a solid phase assay and for ligand binding by a radiobinding assay and an enzyme linked immunosorbent assay. The half-life of immune complexes formed with radioligand was measured and, as expected, correlated with affinity as measured by the method of Scatchard. Antibody titres determined by enzyme linked immunosorbent assay did not correlate to those measured by radiobinding assay. IgG concentration correlated to enzyme linked immunosorbent assay titres but not to radiobinding assay titres. Finally, a significant correlation was found between radiobinding assay titre and the product of enzyme linked immunosorbent assay titre by the period of immune complexes. It is concluded that, except for very low affinity antibodies, enzyme linked immunosorbent assay is a capacity assay whereas radiobinding assay is influenced by both antibody concentration and affinity. The former assay is thus best suited to detecting low affinity antibodies whereas the latter is more efficient in the presence of low levels of high affinity antibodies.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Sodoyez JC,Koch M,Lemaire I,Sodoyez-Goffaux F,Rapaille A,François-Gérard C,Sondag Ddoi
10.1007/BF00403281subject
Has Abstractpub_date
1991-07-01 00:00:00pages
463-8issue
7eissn
0012-186Xissn
1432-0428journal_volume
34pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract::Non-obese diabetic mice were treated daily with a mixture of gangliosides from day 30 until day 250 of life or until the mice became diabetic. Ganglioside treatment reduced diabetes incidence from 80-90% to 47% and from 20-30% to zero in female and male mice respectively. Gangliosides did not affect the frequency of p...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00277490
更新日期:1988-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:We determined the effects of 6 years of lifestyle intervention in persons with impaired glucose tolerance (IGT) on the development of retinopathy, nephropathy and neuropathy over a 20 year period. METHODS:In 1986, 577 adults with IGT from 33 clinics in Da Qing, China were randomly assigned by clinic to...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-010-1948-9
更新日期:2011-02-01 00:00:00
abstract::Phosphoinositide 3-kinase (PI3-kinase) plays a crucial role in insulin signal transduction. We studied the molecular mechanism of the insulin-induced activation of PI3-kinase in rat hepatoma Fao cells using an antibody against the 110-kDa catalytic subunit (p110) and two against the 85-kDa regulatory subunit (p85 alph...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00403297
更新日期:1996-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:We sought to determine the risk of diabetes and IGT/IFG with grand multiparity. SUBJECTS, MATERIALS AND METHODS:Women, aged > or =25 years, from the Australian Diabetes, Obesity and Lifestyle Study and the Crossroads Undiagnosed Disease Study (a rural study in Victoria, Australia), participated in a ho...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0276-6
更新日期:2006-07-01 00:00:00
abstract::Studies were made on the effect of the enteroinsular axis on amino acid-induced insulin and glucagon secretion during hyperglycaemia in man. The responses of plasma immunoreactive insulin, C-peptide, and immunoreactive glucagon to arginine infusion were investigated in nine healthy subjects after induction of hypergly...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00274792
更新日期:1987-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:The role of infections in the aetiology of Type I diabetes is controversial. Certain enteroviral infections might be involved in triggering the beta-cell destruction but insufficient exposure to early infections might increase the risk. We studied how the number of infections experienced during several ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051517
更新日期:2000-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to evaluate the prognostic significance of autoantibodies to IA-2beta (IA2betaA) in a large, well-characterised population of islet cell antibody (ICA)-positive relatives followed for 5 years in the European Nicotinamide Diabetes Intervention Trial. METHODS:Autoantibodies to i...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0912-9
更新日期:2008-03-01 00:00:00
abstract::A previous study of low density lipoprotein metabolism by cultured cells focused on the metabolism of normal lipoproteins in vitro by fibroblasts isolated from diabetic patients. No abnormalities were found. We have followed the opposite approach. Using normal human fibroblasts as test cells we compared the metabolism...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00282585
更新日期:1982-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:The Ca2+/calmodulin-dependent protein kinase II (CaMK II) is highly expressed in pancreatic islets and associated with insulin secretion vesicles. The suppression of CaMK II disturbs insulin secretion and insulin gene expression. There are four isoforms of CaMK II, alpha to delta, that are expressed fro...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051330
更新日期:2000-04-01 00:00:00
abstract::Present-day treatments for people that are insulin dependent require multiple insulin injections, sometimes with an insulin pump, coupled with regular blood glucose monitoring. The availability of modified insulins, each with peaks of activity at varying times, has improved diabetes management. On the other hand, ther...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-020-05367-2
更新日期:2021-01-16 00:00:00
abstract:AIMS/HYPOTHESIS:Sympathetic nerves influence islet hormone levels in the circulation. Insights into islet sympathetic innervation and its remodelling in diabetes may impact future therapeutics. However, standard immunohistochemistry and microtome-based microscopy cannot provide an integral view of the islet neurovascul...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2699-6
更新日期:2012-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Cardiac autonomic nervous dysfunction (CAND) raises the risk of mortality, but the glycaemic threshold at which it develops is unclear. We aimed to determine the prevalence of, risk factors for and impact of CAND in glucose intolerance and diabetes. METHODS:Among 1,332 eligible participants aged 55-74 ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3534-7
更新日期:2015-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:Aggregation of islet amyloid polypeptide (IAPP) to form amyloid contributes to beta cell dysfunction in type 2 diabetes. Human but not non-amyloidogenic rodent IAPP induces islet macrophage proIL-1β synthesis. We evaluated the effect of IL-1 receptor antagonist (IL-1Ra) on islet inflammation and dysfunc...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3447-x
更新日期:2015-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:This study was aimed at the elucidation of the pathogenesis of glucotoxicity, i.e. the mechanism whereby hyperglycaemia damages pancreatic beta cells. The identification of pathways in the process may help identify targets for beta cell-protective therapy. Carbohydrate response element-binding protein (...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2506-4
更新日期:2012-06-01 00:00:00
abstract::Plasma glucose control and arterial pressure were assessed in 28 Type 1 (insulin-dependent) diabetic patients with different degrees of micro-albuminuria. They were divided into two groups according to their urinary albumin excretion rate: a low micro-albuminuria group (n = 16) with albumin excretion ranging between 1...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00262209
更新日期:1984-06-01 00:00:00
abstract::Analysis of the plasma from a totally pancreatectomized patient, with antiserum 30 K, has demonstrated basal glucagon immunoreactivity (GIR) levels in the normal range (80-110 pg/ml). Neither i. v. arginine nor oral glucose affected these GIR values, thus indicating the absence of functioning pancreatic or gastrointes...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01220639
更新日期:1976-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:There has been much focus on the potential role of mitochondria in the aetiology of type 2 diabetes and the metabolic syndrome, and many case-control mitochondrial association studies have been undertaken for these conditions. We tested for a potential association between common mitochondrial variants a...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1510-9
更新日期:2009-11-01 00:00:00
abstract::We have investigated whether the previously demonstrated stimulatory actions of growth hormone on DNA synthesis and (pro)insulin biosynthesis and release of isolated adult rat islets of Langerhans are mediated by an autocrine release of somatomedin-C/insulin-like growth factor I (SM-C/IGF I). In medium containing 1% f...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00265093
更新日期:1989-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:The study aimed to assess, in multiple populations, the role of HLA alleles on early and late age at onset of type 1 diabetes. METHODS:Stepwise linear regression models were used to determine which HLA class I and class II risk alleles to include. High-resolution genotyping data for patients from the T...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2608-z
更新日期:2012-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glucose-dependent insulinotropic polypeptide (GIP) is a peptide hormone released from gut K cells. While the predominant form is GIP(1-42), a shorter form, GIP(1-30), is produced by pancreatic alpha cells and promotes insulin secretion in a paracrine manner. Here, we elucidated whether GIP(1-30) express...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3842-y
更新日期:2016-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Experimental studies have suggested that apoptosis is involved in diabetic embryopathy through oxidative stress. However, the precise mechanism of diabetic embryopathy is not yet clear. Thioredoxin (TRX) is a small, ubiquitous, multifunctional protein, which has recently been shown to protect cells from...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1784-y
更新日期:2010-09-01 00:00:00
abstract::The capacity of reovirus type 3 to infect insulin-producing B cells was studied in human pancreatic cell cultures. Antibody to reovirus was labelled with fluorescein isothiocyanate and antibody to insulin was labelled with tetramethyl rhodamine isothiocyanate. By using a double-labelled immunofluorescent antibody tech...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00253408
更新日期:1981-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Pharmacological augmentation of glucagon-like peptide 1 receptor signalling by dipeptidyl peptidase 4 (DPP-4) inhibition reduced intestinal lipoprotein secretion in experimental studies, suggesting that DPP-4 inhibitors may ameliorate dyslipidaemia and thus reduce cardiovascular risk in patients with ty...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00125-011-2447-3
更新日期:2012-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Novel strategies to stimulate the expansion of beta cell mass in situ are warranted for diabetes therapy. The aim of this study was to elucidate the secretome of human bone marrow (BM)-derived multipotent stromal cells (MSCs) with documented islet regenerative paracrine function. We hypothesised that re...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-017-4355-7
更新日期:2017-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:We investigated of the effects of fatty acid composition of the maternal diet on fetal and postnatal growth, morphology of the pancreas and glucose metabolism and muscle hexosamine concentrations in the adult offspring of rats. METHODS:High-fat diets enriched with either saturated or unsaturated fatty ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-002-0918-2
更新日期:2002-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:We analysed whether the prognosis of a first acute coronary syndrome (ACS) in patients treated for type 2 diabetes has improved. We also compared the trends in patients with and without diabetes. METHODS:We used national registers to identify all patients with clinically known type 2 diabetes in Finlan...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1606-2
更新日期:2010-03-01 00:00:00
abstract::Monolayer cell cultures from pancreatic islets of aging 129/J strain diabetes (db3J/db3J) and lean littermate control mice were tested for differences in glucagon and insulin secretion in either serum-free Eagle's minimal essential medium (MEM) or Dulbecco's modified minimal essential medium (DMEM). There was a highly...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00258314
更新日期:1980-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Knowledge of the within-subject variability of a parameter is required to properly design and calculate sample sizes for longitudinal studies. We sought to determine the day-to-day variability of measures of beta cell function derived from an OGTT. METHODS:Thirty-seven adults (13 with normal glucose to...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-007-0819-5
更新日期:2007-12-01 00:00:00
abstract::Insulin resistance is a significant pathogenetic factor in the development of non-insulin-dependent diabetes mellitus (NIDDM). A new class of drugs, the thiazolidinediones, have been shown to lower blood glucose levels without stimulating insulin secretion. We report the metabolic effect of the thiazolidinedione, darg...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/BF00401763
更新日期:1995-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:Rho GTPases (Ras-related C3 botulinum toxin substrate 1 [Rac1] and cell division cycle 42 [Cdc42]) have been shown to regulate glucose-stimulated insulin secretion (GSIS) via cytoskeletal remodelling, trafficking and fusion of insulin-secretory granules with the plasma membrane. GTP loading of these G p...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3707-4
更新日期:2015-11-01 00:00:00