Abstract:
:We report here the desensitization and internalization of the relaxin receptor (RXFP1) after agonist activation in both primary human decidual cells and HEK293 cells stably transfected with RXFP1. The importance of beta-arrestin 2 in these processes has also been demonstrated. Thus, in HEK-RXFP1 cells the desensitization of RXFP1 was significantly increased when beta-arrestin 2 was overexpressed. After relaxin activation, beta-arrestin 2 was translocated to the cell membrane and RXFP1 underwent rapid internalization. We have previously shown that RXFP1 forms dimers/oligomers during its biosynthesis and trafficking to the plasma membrane, we now show that internalization of RXFP1 occurs through this dimerization/oligomerization. In nonagonist stimulated cells, it is known that the majority of the RXFP1 is located intracellularly and was confirmed in the cells used here. Constitutive internalization of RXFP1 could account for this and indeed, slow but robust constitutive internalization, which was increased after agonist stimulation was demonstrated. A carboxyl-terminal deleted RXFP1 variant had a similar level of constitutive agonist-independent internalization as the wild-type RXFP1 but lost sensitivity to agonist stimulation. This demonstrated the importance of the carboxyl terminus in agonist-stimulated receptor internalization. These data suggest that the autocrine/paracrine actions of relaxin in the decidua are under additional controls at the level of expression of its receptor on the surface of its target cells.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Kern A,Bryant-Greenwood GDdoi
10.1210/en.2008-1385subject
Has Abstractpub_date
2009-05-01 00:00:00pages
2419-28issue
5eissn
0013-7227issn
1945-7170pii
en.2008-1385journal_volume
150pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Galanin (GAL) has recently emerged as an important neuroendocrine regulator which participates in the control of several pituitary and hypothalamic hormones. Our earlier observation that GAL stimulates LHRH release from nerve terminals of the median eminence as well as basal LH and LHRH-induced LH secretion from pitui...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.7678800
更新日期:1993-02-01 00:00:00
abstract::The inhibitor of apoptosis proteins (IAPs) constitutes a family of highly conserved apoptosis suppressor proteins that were originally identified in baculoviruses. Although IAP homologs have recently been demonstrated to suppress apoptosis in mammalian cells, their expression and role in human ovarian epithelial cance...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7897
更新日期:2001-01-01 00:00:00
abstract::Subcellular preparations from rat liver, brain, and kidney and from human liver were tested for their ability to desulfate T3 sulfate (T3SO4). Activity was found associated with the microsomal fraction: rat liver was the most active, hydrolyzing 76 pmol/min.mg protein of T3SO4 while preparations from rat kidney and br...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1195
更新日期:1988-04-01 00:00:00
abstract::We have examined the regulation of an insulin-like growth factor-binding protein-3 (IGFBP-3) protease secreted by MCF-7 human breast cancer cells using a ligand-binding assay that relies on the decrease in affinity for des(1-3)IGF-I that occurs when IGFBP-3 becomes proteolyzed. IGFBP-3 protease activity was not altere...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.9.7684
更新日期:2000-09-01 00:00:00
abstract::Peptidergic neurons synthesizing kisspeptin (KP) and neurokinin B (NKB) in the hypothalamic infundibular nucleus have been implicated in negative sex steroid feedback to GnRH neurons. In laboratory rodents, testosterone decreases KP and NKB expression in this region. In the present study, we addressed the hypothesis t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1739
更新日期:2012-11-01 00:00:00
abstract::Hypothalamic inflammation and gliosis are proposed to participate in the pathogenesis of high-fat diet-induced obesity. Because other factors and nutrients also induce weight gain and adiposity, we analyzed the inflammatory and glial responses to a sucrose (S)-enriched diet. Neonatal overnutrition (NON) exacerbates we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2196
更新日期:2013-07-01 00:00:00
abstract::Male rats, kept under a lighting condition of 14-h light, 10-h dark, were subjected to scheduled feeding regimens. Food was available either in the early light phase or the early dark phase. The 24-h rhythms of serum corticosterone and of N-acetylserotonin (NAS) and melatonin (MT) in the pineal and serum were determin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-5-1858
更新日期:1985-05-01 00:00:00
abstract::Diets lacking soy and alpha-alpha derivatives that are considered to be estrogen-free by standard bioassays (uterotrophic assay and vaginal opening) have been revealed to contain considerable amounts of compounds able to transcriptionally activate the estrogen receptors (ERs) and stimulate luciferase expression in sev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0660
更新日期:2005-12-01 00:00:00
abstract::Pharmacological amounts of 25(s),26-dihydroxyvitamin D3 were administered to normal, vitamin D-replete rats in order to assess its pharmacological activity. Treatment with 25(s),26-dihydroxyvitamin D3 (20 micrograms/day for 1 week) caused a marked and significant fall in the circulating concentration of 1,25-dihydroxy...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-5-1671
更新日期:1987-11-01 00:00:00
abstract::The seasonal changes in response to the negative feedback action of estradiol on LH secretion were studied in two groups of ovariectomized Japanese monkeys (Macaca fuscata fuscata; n = 6). Blood samples were collected two to three times a week, and changes in serum concentrations of LH, estradiol, and progesterone wer...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1291
更新日期:1991-03-01 00:00:00
abstract::GnRH neurons are the central regulators of fertility, and their activity is modulated by steroid feedback. In women with hyperandrogenemic infertility and in animal models of these disorders, elevated androgen levels interfere with progesterone (P) negative feedback. Our previous work showed that steroids altered the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1029
更新日期:2006-03-01 00:00:00
abstract::We have investigated the activation of ERK2, a serine/threonine kinase necessary for transmission of mitogenic signals, in cells derived from mouse embryos homozygous for a null mutation of the insulin-like growth factor (IGF)-1R gene (R- cells) and from wild-type littermates (W cells), respectively. Stimulation of qu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.7.6766
更新日期:1999-07-01 00:00:00
abstract::The effects of dopamine on PRL secretion and lysosomal enzyme activity in anterior pituitary tissue from rats selected during various stages of the estrous cycle were examined under in vitro conditions. During the estrous cycle, there was a marked variation in the capacity of dopamine to stimulate the activity of the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-903
更新日期:1981-03-01 00:00:00
abstract::The androgen receptor (AR) in Sertoli cells mediates the actions of testosterone on spermatogenesis. However, the transcription factors responsible for AR gene regulation in Sertoli cells remain unknown. In this study, we determined that nuclear factor-kappaB (NF-kappaB) regulates transcription of AR in primary cultur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0987
更新日期:2004-02-01 00:00:00
abstract::We recently reported that aluminum administration to beagles stimulates uncoupled bone formation in the marrow cavity which increases trabecular bone volume and generates new osseous networks within the axial skeleton. To investigate whether this osteogenic process results from direct stimulation of bone cell replicat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3144
更新日期:1991-06-01 00:00:00
abstract::The effects of electrical and chemical stimulations of the hypothalamus on the plasma levels of glucagon, insulin, and glucose were studied in undisturbed and free-moving rabbits. Electrical stimulation of the ventromedial hypothalamic nucleus (VMH) caused a marked increase in the circulating level of glucagon coincid...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-2-605
更新日期:1981-02-01 00:00:00
abstract::Apoptosis is the process of cellular self-destruction, and genes such as bcl-2 and bax are known to inhibit and promote apoptosis, respectively. In this study, we show that apoptosis can be induced in pancreatic beta-cell lines, and we investigate the apoptotic pathways through the bcl-2 and bax genes and intracellula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5798
更新日期:1998-03-01 00:00:00
abstract::A binding protein for activin was purified from bovine pituitary by affinity chromatography on dextran sulfate-Sepharose CL-4B and activin-Affi-Gel 10. A 52,700-fold purification over the starting crude homogenate was achieved. The purified preparation showed two bands of 36 and 33 kilodalton in sodium dodecyl sulfate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1434
更新日期:1991-03-01 00:00:00
abstract::It is well established that LH has an obligatory role in the acute production of progesterone by the primate corpus luteum in vivo because interruption of LH support to the corpus luteum at any time during the luteal phase is accompanied by an immediate and sustained fall in serum progesterone concentrations. However,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1425410
更新日期:1992-11-01 00:00:00
abstract::We report here 1) that GnRH and a potent GnRH agonist inhibit basal and LH stimulated ovarian androgen biosynthesis in vitro, 2) that the inhibitory effects of GnRH are dose-dependent and completely inhibited by concomitant treatment with a GnRH antagonist and 3) that the GnRH action is very rapid (t1/2 = 10 min) and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-2-661
更新日期:1981-08-01 00:00:00
abstract::Retinoids induce cellular differentiation and inhibit cellular proliferation. Proliferation of human breast carcinoma cells in vitro is markedly inhibited by these compounds. On the other hand, insulin-like growth factors (IGFs) and their receptors seem to be involved in the growth of certain breast carcinoma cells by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-1115
更新日期:1991-02-01 00:00:00
abstract::Parathyroid tissue from patients with hyperparathyroidism (HPT) exhibited reduced immunohistochemical reactivity with monoclonal antiparathyroid antibodies, previously shown to stain intensely the surface of normal human parathyroid cells and to interfere with a receptor mechanism of these cells which is involved in t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2999
更新日期:1988-06-01 00:00:00
abstract::Young, unilaterally nephrectomized, female Sprague-Dawley rats were given daily sc injections of 19-nor-deoxycorticosterone acetate (19-nor-DOCA) in oil at a dosage of 100 micrograms/day for 21 days and twice that amount for a further 11 days. One group drank distilled water and another drank 1% NaCl solution. Compara...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-3-600
更新日期:1979-09-01 00:00:00
abstract::To analyze the roles of insulin receptor substrate (IRS) proteins in insulin-stimulated cell cycle progression, we examined the functions of rat IRS-1 and IRS-3 in Chinese hamster ovary cells overexpressing the human insulin receptor. In this type of cell overexpressing IRS-1 or IRS-3, we showed that: 1) overexpressio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0199
更新日期:2004-12-01 00:00:00
abstract::We have previously shown that placental protein 12 (PP12) is synthesized and secreted by human term pregnancy decidua in vitro. In the present study, fragments of proliferative and secretory phase endometrium were cultured in media in the presence and absence of progesterone (P) and 17 beta-estradiol (E2) for 96 h. Th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-1067
更新日期:1986-03-01 00:00:00
abstract::Estrogens have pronounced effects on thermoregulation, as illustrated by the occurrence of hot flushes secondary to estrogen withdrawal in menopausal women. Because neurokinin B (NKB) gene expression is markedly increased in the infundibular (arcuate) nucleus of postmenopausal women, and is modulated by estrogen withd...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1492
更新日期:2011-12-01 00:00:00
abstract::The involvement of hypothalamic histaminergic neurons in the stress-induced release of peripheral catecholamines was studied in conscious, freely moving male rats. Blood samples were obtained via a catheter in a femoral artery. Intracerebroventricular infusion of histamine (HA; 30 micrograms) increased the plasma conc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1430
更新日期:1990-03-01 00:00:00
abstract::A complementary DNA, uterine-ovarian-specific gene 44 (UO-44), has been isolated from tamoxifen-induced rat uterine complementary DNA library using differential display techniques. UO-44 transcripts are found to be abundant in the uterus and ovary. UO-44 gene expression in the uterus is strictly regulated by estrogens...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8247
更新日期:2001-07-01 00:00:00
abstract::A novel NADPH-dependent cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) was purified by sequential fractionation of rat liver cytosol on Q-Sepharose, phenyl-Sepharose, red-Sepharose, and polyacrylamide gel electrophoresis under nondenaturing conditions. The CTBP had a sedimentation coefficient of 5.1S...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-1701
更新日期:1991-10-01 00:00:00
abstract::The protein thiol-modifying agent arsenite, a potent activator of stress signaling, was used to examine the involvement of MAPKs in the regulation of cardiac substrate uptake. Arsenite strongly induced p38 MAPK phosphorylation in isolated rat cardiac myocytes but also moderately enhanced phosphorylation of p42/44 ERK ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0849
更新日期:2006-11-01 00:00:00