Activin-binding protein is present in pituitary.


:A binding protein for activin was purified from bovine pituitary by affinity chromatography on dextran sulfate-Sepharose CL-4B and activin-Affi-Gel 10. A 52,700-fold purification over the starting crude homogenate was achieved. The purified preparation showed two bands of 36 and 33 kilodalton in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. The ability of each form of the protein to specifically bind activin was determined by ligand blot analysis and binding competition study. Each protein was found to have the same NH2-terminus and its sequence was identical to that of follistatin, which is a specific inhibitor of identical to that of follistatin, which is a specific inhibitor of FSH release. Moreover, the binding protein was shown to inhibit the spontaneous FSH release from cultured pituitary cells as does follistatin. These properties are the same as activin-binding protein that we have obtained from rat ovary. These results support a conclusion that activin-binding protein/follistatin exists also in the pituitary. Activin-binding protein has an ability to inhibit the activin-induced augmentation of FSH release from cultured pituitary cells as does inhibin. However, the inhibitory pattern by the binding protein was quite distinct from that of inhibin, suggesting that there may be different mechanism(s) for their antagonistic actions. Stoichiometric inhibition as shown by gel filtration analysis indicates that activin-binding protein binds activin to form an inactive equimolar complex having neither stimulatory nor inhibitory activity for FSH secretion by the pituitary. These findings suggest that activin is actually involved in FSH secretion in the pituitary and that the activin action in the pituitary is regulated by activin-binding protein/follistatin.






Kogawa K,Nakamura T,Sugino K,Takio K,Titani K,Sugino H




Has Abstract


1991-03-01 00:00:00












  • Tumor necrosis factor-alpha is a potent ACTH secretagogue: comparison to interleukin-1 beta.

    abstract::Tumor necrosis factor-alpha (TNF) and interleukin-1 beta (IL-1) are secreted by activated monocytes and other immune cells. Since IL-1 has been shown to elevate rat plasma ACTH and both of these cytokines induce similar acute-phase responses, the present studies of TNF were undertaken to characterize the ACTH response...


    pub_type: 杂志文章


    authors: Sharp BM,Matta SG,Peterson PK,Newton R,Chao C,Mcallen K

    更新日期:1989-06-01 00:00:00

  • Uroguanylin, an intestinal natriuretic peptide, is delivered to the kidney as an unprocessed propeptide.

    abstract::Orally delivered salt stimulates renal salt excretion more effectively than does iv delivered salt. Although the mechanisms that underlie this "postprandial natriuresis" are poorly understood, the peptide uroguanylin (UGn) is thought to be a key mediator. However, the lack of selective assays for UGn gene products has...


    pub_type: 杂志文章


    authors: Moss NG,Fellner RC,Qian X,Yu SJ,Li Z,Nakazato M,Goy MF

    更新日期:2008-09-01 00:00:00

  • Disrupted TSH Receptor Expression in Female Mouse Lung Fibroblasts Alters Subcellular IGF-1 Receptor Distribution.

    abstract::A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts as has the noncanonical behavior of IGF-...


    pub_type: 杂志文章


    authors: Atkins SJ,Lentz SI,Fernando R,Smith TJ

    更新日期:2015-12-01 00:00:00

  • Interactions of dopamine and neurotensin on calcium fluxes and prolactin release in normal rat pituitary cells.

    abstract::We studied the role of calcium in dopaminergic control of the neuroendocrine effects of neurotensin. In primary cultures of dispersed normal female rat anterior pituitary cells the interactions of dopamine and neurotensin were examined with reference to the rate of PRL release, the magnitude of 45Ca2+ uptake, the rate...


    pub_type: 杂志文章


    authors: Login IS,Kuan SI,Judd AM,MacLeod RM

    更新日期:1990-10-01 00:00:00

  • Pituitary corticotrope tumor (AtT20) cells as a model system for the study of early inhibition by glucocorticoids.

    abstract::The utility of the established ACTH secreting mouse pituitary tumor cell line AtT20 for investigating early glucocorticoid inhibition was examined. Three different strains of the cell line D1, D16v, and D16:16, respectively, were analyzed. In initial studies CRF and phorbol esters were used as secretagogues to examine...


    pub_type: 杂志文章


    authors: Woods MD,Shipston MJ,Mullens EL,Antoni FA

    更新日期:1992-12-01 00:00:00

  • Human prolactin gene promoter regulation by estrogen: convergence with tumor necrosis factor-alpha signaling.

    abstract::Estrogens have been implicated in the regulation of prolactin gene expression in man, although previous studies have not defined the molecular mechanism whereby estradiol activates the human prolactin gene promoter (hPrl). We found that estradiol induced a reproducible 1.8-fold activation of the hPrl gene promoter, us...


    pub_type: 杂志文章


    authors: Adamson AD,Friedrichsen S,Semprini S,Harper CV,Mullins JJ,White MR,Davis JR

    更新日期:2008-02-01 00:00:00

  • EP24.15 as a Potential Regulator of Kisspeptin Within the Neuroendocrine Hypothalamus.

    abstract::The neuropeptide kisspeptin (Kiss1) is integral to the advent of puberty and the generation of cyclical LH surges. Although many complex actions of Kiss1 are known, the mechanisms governing the processing/regulation of this peptide have not been unveiled. The metallo enzyme, endopeptidase 24.15 (thimet oligopeptidase)...


    pub_type: 杂志文章


    authors: Woitowich NC,Philibert KD,Leitermann RJ,Wungjiranirun M,Urban JH,Glucksman MJ

    更新日期:2016-02-01 00:00:00

  • Interleukin-2 driven nuclear translocation of prolactin in cloned T-lymphocytes.

    abstract::The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into ...


    pub_type: 杂志文章


    authors: Clevenger CV,Sillman AL,Prystowsky MB

    更新日期:1990-12-01 00:00:00

  • Defining the Role of Estrogen Receptor β in the Regulation of Female Fertility.

    abstract::Estrogens are essential hormones for the regulation of fertility. Cellular responses to estrogens are mediated by estrogen receptor α (ESR1) and estrogen receptor β (ESR2). In mouse and rat models, disruption of Esr1 causes infertility in both males and females. However, the role of ESR2 in reproductive function remai...


    pub_type: 杂志文章


    authors: Rumi MAK,Singh P,Roby KF,Zhao X,Iqbal K,Ratri A,Lei T,Cui W,Borosha S,Dhakal P,Kubota K,Chakraborty D,Vivian JL,Wolfe MW,Soares MJ

    更新日期:2017-07-01 00:00:00

  • Glycogen synthase kinase-3beta activity is required for androgen-stimulated gene expression in prostate cancer.

    abstract::Despite the specificity inferred by its name, glycogen synthase kinase (GSK)-3beta is an important kinase with a plethora of significant cellular targets, including cytoskeletal proteins and transcription factors, and its activity is regulated by phosphorylation on tyrosine/serine residues. As part of our efforts to d...


    pub_type: 杂志文章


    authors: Liao X,Thrasher JB,Holzbeierlein J,Stanley S,Li B

    更新日期:2004-06-01 00:00:00

  • Discovery of a novel superpotent and selective melanocortin-4 receptor antagonist (HS024): evaluation in vitro and in vivo.

    abstract::Several novel cyclic MSH analogs were synthesized, and their binding properties were tested on cells transiently expressing the human melanocortin-1 (MC1), MC3, MC4, and MC5 receptors. We discovered a novel substance (HS024) that showed about 20-fold selectivity and very high affinity (Ki = 0.29 nM) for the MC4 recept...


    pub_type: 杂志文章


    authors: Kask A,Mutulis F,Muceniece R,Pähkla R,Mutule I,Wikberg JE,Rägo L,Schiöth HB

    更新日期:1998-12-01 00:00:00

  • Synergistic action of purified alpha-fetoprotein and growth factors on the proliferation of porcine granulosa cells in monolayer culture.

    abstract::alpha-Fetoprotein (AFP) is present in high levels in fetal fluids, certain neoplasias, and regenerating liver. Although AFP's physiological role remains an enigma, we have recently demonstrated mitogenic activity for AFP. Using a primary monolayer culture system, we have further investigated the proliferative activity...


    pub_type: 杂志文章


    authors: Keel BA,Eddy KB,Cho S,May JV

    更新日期:1991-07-01 00:00:00

  • Expression of prolactin (PRL) receptor gene and PRL-binding sites in rabbit intestinal epithelial cells.

    abstract::Specific binding sites for PRL were identified on isolated intestinal epithelial cells from both male and female rabbits. These receptors exhibited a very high affinity for PRL (Kd = 5 x 10(-11) M) and were immunologically very similar to the well characterized mammary PRL receptor. About 3500 PRL receptors/cell were ...


    pub_type: 杂志文章


    authors: Dusanter-Fourt I,Belair L,Gespach C,Djiane J

    更新日期:1992-05-01 00:00:00

  • Kisspeptin is present in ovine hypophysial portal blood but does not increase during the preovulatory luteinizing hormone surge: evidence that gonadotropes are not direct targets of kisspeptin in vivo.

    abstract::There is strong evidence that kisspeptin acts to regulate GnRH secretion, but whether there is also a component of action on the gonadotropes is not clear. Using quantitative RT-PCR, we found that G protein-coupled receptor-54 mRNA is expressed in ovine pituitary cell fractions enriched for gonadotropes as well as in ...


    pub_type: 杂志文章


    authors: Smith JT,Rao A,Pereira A,Caraty A,Millar RP,Clarke IJ

    更新日期:2008-04-01 00:00:00

  • Cell-specific activation of the human skeletal alpha-actin by androgens.

    abstract::Although it is evident that androgens increase muscle mass and strength, little is known about the critical molecular targets of androgens in skeletal muscle. In rodents, the skeletal alpha-actin gene is a tissue-specific gene expressed only in the levator ani and other skeletal muscles but not in the prostate or prep...


    pub_type: 杂志文章


    authors: Hong MH,Sun H,Jin CH,Chapman M,Hu J,Chang W,Burnett K,Rosen J,Negro-Vilar A,Miner JN

    更新日期:2008-03-01 00:00:00

  • The effect of carbonyl cyanide trifluoromethoxyphenylhydrazone and methylamine on the processing and secretion of the glycoprotein hormone chorionic gonadotropin by human choriocarcinoma cells.

    abstract::Carbonyl cyanide trifluoromethoxyphenylhydrazone (FCCP), a protonophore, and methylamine, a weak base, agents that dissipate hydrogen gradients across cellular membranes, were used to probe the coupling of hydrogen gradients to the processing and secretion of the glycoprotein hormone hCG by human choriocarcinoma cells...


    pub_type: 杂志文章


    authors: Peters BP,Krzesicki RF,Perini F,Ruddon RW

    更新日期:1986-07-01 00:00:00

  • Bone Morphogenetic Protein-4 (BMP4): A Paracrine Regulator of Human Adrenal C19 Steroid Synthesis.

    abstract::Bone morphogenetic proteins (BMPs) comprise one of the largest subgroups in the TGF-β ligand superfamily. We have identified a functional BMP system equipped with the ligand (BMP4), receptors (BMP type II receptor, BMP type IA receptor, also called ALK3) and the signaling proteins, namely the mothers against decapenta...


    pub_type: 杂志文章


    authors: Rege J,Nishimoto HK,Nishimoto K,Rodgers RJ,Auchus RJ,Rainey WE

    更新日期:2015-07-01 00:00:00

  • Genomic structure and characterization of the 5'-flanking region of the human ghrelin gene.

    abstract::Ghrelin, an endogenous ligand for the GH secretagogue receptor, induces GH secretion, food intake, and positive energy balance. Although ghrelin exhibits a variety of hormonal actions, the mechanisms regulating ghrelin expression and secretion remain unclear. To understand regulation of human ghrelin gene expression, ...


    pub_type: 杂志文章


    authors: Kanamoto N,Akamizu T,Tagami T,Hataya Y,Moriyama K,Takaya K,Hosoda H,Kojima M,Kangawa K,Nakao K

    更新日期:2004-09-01 00:00:00

  • Inhibition of cisplatin-induced lipid catabolism and weight loss by ghrelin in male mice.

    abstract::Cachexia, defined as an involuntary weight loss ≥ 5%, is a serious and dose-limiting side effect of chemotherapy that decreases survival in cancer patients. Alterations in lipid metabolism are thought to cause the lipodystrophy commonly associated with cachexia. Ghrelin has been proposed to ameliorate the alterations ...


    pub_type: 杂志文章


    authors: Garcia JM,Scherer T,Chen JA,Guillory B,Nassif A,Papusha V,Smiechowska J,Asnicar M,Buettner C,Smith RG

    更新日期:2013-09-01 00:00:00

  • Huwe1 Regulates the Establishment and Maintenance of Spermatogonia by Suppressing DNA Damage Response.

    abstract::Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of the u...


    pub_type: 杂志文章


    authors: Fok KL,Bose R,Sheng K,Chang CW,Katz-Egorov M,Culty M,Su S,Yang M,Ruan YC,Chan HC,Iavarone A,Lasorella A,Cencic R,Pelletier J,Nagano M,Xu W,Wing SS

    更新日期:2017-11-01 00:00:00

  • Hexosamine biosynthesis pathway flux contributes to insulin resistance via altering membrane phosphatidylinositol 4,5-bisphosphate and cortical filamentous actin.

    abstract::We recently found that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2))-regulated filamentous actin (F-actin) polymerization was diminished in hyperinsulinemic cell culture models of insulin resistance. Here we delineated whether increased glucose flux through the hexosamine biosynthesis pathway (HBP) ca...


    pub_type: 杂志文章,收录出版


    authors: Bhonagiri P,Pattar GR,Horvath EM,Habegger KM,McCarthy AM,Elmendorf JS

    更新日期:2009-04-01 00:00:00

  • The mouse prolactin gene family locus.

    abstract::In the mouse, there is a large family of paralogous genes closely related to PRL. The objective of this report was to investigate the organization of the mouse PRL gene family locus. PRL family genes reside on chromosome 13 of the mouse genome. The PRL gene family members were localized to a series of overlapping bact...


    pub_type: 杂志文章


    authors: Wiemers DO,Shao LJ,Ain R,Dai G,Soares MJ

    更新日期:2003-01-01 00:00:00

  • Effects of thyrotropin and cholera toxin on the thyroidal adenylate cyclase-adenosine 3',5'-monophosphate system.

    abstract::The present experiments examined the relationship between cholera toxin and TSH stimulation of the adenylate cyclase system in bovine thyroid tissue. Preincubation of thyroid slices for 20 min at 4 C with a maximal concentration of cholera toxin (100 microgram/ml) did not impair the subsequent stimulation of cAMP by s...


    pub_type: 杂志文章


    authors: Holmes SD,Titus G,Chou M,Field JB

    更新日期:1980-12-01 00:00:00

  • In vitro inhibition of testosterone biosynthesis by ketoconazole.

    abstract::Oral ketoconazole has been demonstrated to lower plasma testosterone in man. Measurement of blood precursors of testosterone suggest that ketoconazole may have its effect inhibiting the 17,20-desmolase enzyme within the testis. To substantiate this, a series of in vitro experiments was conducted using the rat testis t...


    pub_type: 杂志文章


    authors: Sikka SC,Swerdloff RS,Rajfer J

    更新日期:1985-05-01 00:00:00

  • Mechanism of action of estrogen on intramembranous bone formation: regulation of osteoblast differentiation and activity.

    abstract::Dynamic bone histomorphometry, [3H]thymidine radioautography, and Northern analysis for bone matrix proteins and insulin-like growth factor-I (IGF-I) were performed in calvariae of ovariectomized (OVX) and estrogen-treated OVX rats. Treatment of OVX rats with diethylstilbestrol (DES) for 2 weeks reduced the periosteal...


    pub_type: 杂志文章


    authors: Turner RT,Backup P,Sherman PJ,Hill E,Evans GL,Spelsberg TC

    更新日期:1992-08-01 00:00:00

  • A revised role for P-glycoprotein in the brain distribution of dexamethasone, cortisol, and corticosterone in wild-type and ABCB1A/B-deficient mice.

    abstract::The ABCB1-type multidrug resistance efflux transporter P-glycoprotein (P-gp) has been hypothesized to regulate hypothalamic-pituitary-adrenal axis activity by limiting the access of glucocorticoids to the brain. In vivo systemic administration studies using P-gp-deficient mice have shown increased glucocorticoid entry...


    pub_type: 杂志文章


    authors: Mason BL,Pariante CM,Thomas SA

    更新日期:2008-10-01 00:00:00

  • Effects of protein kinase C activation on inositol phosphate generation and intracellular Ca2+ mobilization in bovine parathyroid cells.

    abstract::Activators of protein kinase C, such as phorbol myristate acetate (PMA) and the synthetic diacylglycerol dioctanoylglycerol (diC8), either stimulate or inhibit PTH release depending on the extracellular Ca2+ concentration. By increasing PTH release at high extracellular Ca2+, these agents, in effect, block high Ca2(+)...


    pub_type: 杂志文章


    authors: Shoback DM,Chen TH

    更新日期:1990-07-01 00:00:00

  • Site of androgen inhibition of follicle-stimulating hormone-stimulated progesterone production in porcine granulosa cells.

    abstract::In granulosa cells derived from medium-sized porcine follicles, certain androgens have been shown to inhibit FSH-stimulated progesterone synthesis. To determine the site at which this inhibition takes place, the effects of androgens on FSH- and (Bu)2cAMP-stimulated pregnenolone and progesterone syntheses were examined...


    pub_type: 杂志文章


    authors: Lischinsky A,Evans G,Armstrong DT

    更新日期:1983-12-01 00:00:00

  • Mechanism of induction of delta 5-3 beta-hydroxysteroid dehydrogenase-isomerase activity in rat adrenocortical cells by corticotropin.

    abstract::The regulation of delta 5-3 beta-hydroxysteroid dehydrogenase-isomerase (3 beta-HSD) was studied in primary cultures of rat adrenocortical cells. In the absence of ACTH, this enzymic activity was found to decay with a half-life of 3.1 days, which was similar to the half-life of the enzyme activity induced by ACTH in v...


    pub_type: 杂志文章


    authors: Rybak SM,Ramachandran J

    更新日期:1982-08-01 00:00:00

  • Androgen-regulated expression of a novel member of the aldo-keto reductase superfamily in regrowing rat prostate.

    abstract::The rat prostate is dependent on androgen for normal growth and differentiation. In addition, the organ undergoes rapid cell death upon withdrawal of androgen on castration, and the atrophied tissue is capable of regrowth after androgen replacement in adult animals. In our search for novel factor(s) that participate i...


    pub_type: 杂志文章


    authors: Nishi N,Shoji H,Miyanaka H,Nakamura T

    更新日期:2000-09-01 00:00:00