Abstract：
:To assist haematophagy, Rhodnius prolixus produces several bioactive molecules in its saliva which it injects into the host skin. The most abundant of these molecules are the nitrophorins (NPs). In this work, we reduced the expression of NP1-4 in the saliva of R. prolixus by RNAi and evaluated the subsequent feeding performance of the bugs using the cibarial pump electromyogram either on the dorsal skin or on the tail vein of the mice. NPs salivary mRNA was reduced by >99% in comparison to controls. Saliva from knockdown nymphs also presented 82% less haemproteins while the total protein was not reduced. Knockdown nymphs feeding on the skin had lower ingestion rates mainly due to the longer cumulative probing time and lower cibarial pump frequency. Another difference was that knockdown insects bit approximately 5 times more. No differences were observed between groups fed on the tail vein. When the feeding sites were compared, nymphs fed on the tail vein had higher effective ingestion rates. These findings endorse the importance of the NPs for the ability of bugs to complete the meal in a short total contact time with a low number of bites, decreasing the perception of the insect by the host.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Araujo RN,Soares AC,Paim RM,Gontijo NF,Gontijo AF,Lehane MJ,Pereira MHdoi
10.1016/j.ibmb.2008.10.002subject
Has Abstractpub_date
2009-02-01 00:00:00pages
83-9issue
2eissn
0965-1748issn
1879-0240pii
S0965-1748(08)00179-3journal_volume
39pub_type
杂志文章abstract：:In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (S...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2010.04.009
更新日期：2010-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00004-7
更新日期：2000-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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abstract：:Sex-pheromone production in the night flying female moth, Helicoverpa armigera is under neuroendocrine control due to the timely release of Pheromone Biosynthesis-Activating Neuropeptide (PBAN). Males orient to the females by upwind anemotaxis which usually leads to a successful mating. During copulation insect males ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2011-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
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journal_title：Insect biochemistry and molecular biology
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更新日期：2002-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00077-6
更新日期：1999-11-01 00:00:00
abstract：:A bioinformatics-based investigation of three insect species with completed genome sequences has revealed that insect chitinase-like proteins (glycosylhydrolase family 18) are encoded by a rather large and diverse group of genes. We identified 16, 16 and 13 putative chitinase-like genes in the genomic databases of the...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.06.010
更新日期：2008-04-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.11.003
更新日期：2017-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.10.016
更新日期：2016-01-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.01.008
更新日期：2015-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.12.008
更新日期：2016-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.05.004
更新日期：2012-09-01 00:00:00
abstract：:The participation of eicosanoids and second messengers on the regulation of RHBP endocytosis by the ovaries was investigated, using [(125)I]RHBP in experiments in vivo and in vitro. Addition of PGE(2) (one of the products of the cyclooxygenase pathway) decreased in vitro the uptake of RHBP by 35%. The rate of RHBP end...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(01)00132-1
更新日期：2002-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2002-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2007-10-01 00:00:00
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更新日期：2001-04-27 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2005-08-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2006-12-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2014.03.003
更新日期：2014-05-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.10.008
更新日期：2008-02-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.05.012
更新日期：2015-08-01 00:00:00
abstract：:The cDNAs encoding an intestinal defensin (def1) and lysozyme (lys1) of the reduviid bug Triatoma brasiliensis have been amplified by PCR using specific oligonucleotide primers and 5'- and 3'-RACE, cloned and sequenced. The 576 bp clone has an open reading frame of 282 bp and encodes a pre-prodefensin with 94 amino ac...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2006.04.003
更新日期：2006-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.103179
更新日期：2019-08-01 00:00:00
abstract：:C proteins represent one of the four major protein groups secreted by the tubular accessory glands of male mealworm beetles (Tenebrio molitor). They are basic proteins with an apparent molecular mass of 21.9 kDa. In this paper we present the deduced amino-acid sequence of two, almost identical C proteins, termed C1 an...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90119-8
更新日期：1994-01-01 00:00:00
