当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Is the mature endotoxin Cry1Ac from Bacillus thuringiensis inactivated by a coagulation reaction in the gut lumen of resistant Helicoverpa armigera larvae?

Is the mature endotoxin Cry1Ac from Bacillus thuringiensis inactivated by a coagulation reaction in the gut lumen of resistant Helicoverpa armigera larvae?

Abstract：

:Bacillus thuringiensis endotoxins (Bt-toxins) are the most important biopesticides used in controlling insect pests and vectors of diseases. The emergence of widespread resistance to Bt in some insect species is a serious threat to agricultural production. Analysis of Bt-resistant and susceptible laboratory strains of Helicoverpa armigera revealed elevated immune responses involving increased melanization and the presence of a soluble toxin-binding glycoprotein in the hemolymph and gut lumen of the resistant strain. We propose a resistance mechanism against toxins based on a systemic immune-induction that can be transmitted to the next generation by a maternal effect.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Ma G,Roberts H,Sarjan M,Featherstone N,Lahnstein J,Akhurst R,Schmidt O

doi

10.1016/j.ibmb.2005.02.011

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

729-39

issue

7

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(05)00065-2

journal_volume

35

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • Identification of a Bombyx collagenous protein with multiple short domains of Gly-Xaa-Yaa repeats: cDNA characterization and regulation of expression.

    abstract：:Using a differential screening strategy, a 2 kb cDNA was isolated from a differentiating Bombyx mori imaginal wing discs library. The available reading frame of 1710 bp specifies a conceptual protein with two distinct regions: a 395 amino acid region corresponding to 105 repetitions of the Gly-Xaa-Yaa triple-helical f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(96)00032-x

    authors： Chareyre P,Besson MT,Fourche J,Bosquet G

    更新日期：1996-07-01 00:00:00

  • Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac.

    abstract：:Although many insect cell lines derived from various tissues are available, it is unclear whether endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the present study, we demonstrated that the ovaries-derived Spodoptera litura Sl-HP cell line was susceptible to act...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.01.014

    authors： Chen Z,He F,Xiao Y,Liu C,Li J,Yang Y,Ai H,Peng J,Hong H,Liu K

    更新日期：2015-04-01 00:00:00

  • Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori.

    abstract：:We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholin...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2009.12.015

    authors： Tsubota T,Shimomura M,Ogura T,Seino A,Nakakura T,Mita K,Shinoda T,Shiotsuki T

    更新日期：2010-02-01 00:00:00

  • Rhodnius heme-binding protein (RHBP) is a heme source for embryonic development in the blood-sucking bug Rhodnius prolixus (Hemiptera, Reduviidae).

    abstract：:We have previously shown that Rhodnius prolixus' eggs and hemolymph are pink due to the presence of the hemeprotein Rhodnius heme-binding protein (RHBP). In the hemolymph it functions as an antioxidant. Nevertheless, its function in eggs has not been determined. Here we present evidence that RHBP is a source of heme f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00163-1

    authors： Braz GR,Moreira MF,Masuda H,Oliveira PL

    更新日期：2002-04-01 00:00:00

  • Artificial miRNA-mediated silencing of ecdysone receptor (EcR) affects larval development and oogenesis in Helicoverpa armigera.

    abstract：:The insect pests are real threat to farmers as they affect the crop yield to a great extent. The use of chemical pesticides for insect pest control has always been a matter of concern as they pollute the environment and are also harmful for human health. Bt (Bacillus thuringensis) technology helped the farmers to get ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2016.07.009

    authors： Yogindran S,Rajam MV

    更新日期：2016-10-01 00:00:00

  • Armigeres subalbatus prophenoloxidase III: Cloning, characterization and potential role in morphogenesis.

    abstract：:It has long been suggested that phenoloxidases (POs) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning and melanotic encapsulation of pathogens. Here we report that a mosquito PO, designated Armigeres subalbatus prophenoloxidase III (As-pro-PO III)...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.10.007

    authors： Tsao IY,Lin US,Christensen BM,Chen CC

    更新日期：2009-02-01 00:00:00

  • The shrimp FAMeT cDNA is encoded for a putative enzyme involved in the methylfarnesoate (MF) biosynthetic pathway and is temporally expressed in the eyestalk of different sexes.

    abstract：:Methylfarnesoate (MF), an analogue of the insect juvenile hormone III, has been implicated to play a vital role in the regulation of the growth and reproductive development in crustaceans. Farnesoic acid O-methyltransferase (FAMeT) is the key enzyme involved in catalyzing the final step in the MF biosynthetic pathway....

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00060-1

    authors： Silva Gunawardene YI,Chow BK,He JG,Chan SM

    更新日期：2001-10-01 00:00:00

  • Biosynthetic pathway of arachidonic acid in Spodoptera exigua in response to bacterial challenge.

    abstract：:Eicosanoids play crucial roles in mediating insect immune responses. In vertebrates, phospholipase A2 (PLA2) releases arachidonic acid (AA) from phospholipids (PLs) for biosynthesis of various eicosanoids. However, little AA is found in PLs of lepidopteran insects. Spodoptera exigua, a lepidopteran insect, is known to...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2019.103179

    authors： Hasan MA,Ahmed S,Kim Y

    更新日期：2019-08-01 00:00:00

  • An N-acetyllactosamine-specific lectin, PFA, isolated from a moth (Phalera flavescens), structurally resembles an invertebrate-type lysozyme.

    abstract：:PFA (Phalera flavescens agglutinin) lectin purified from larvae of the lobster moth (P. flavescens) shows a strong binding ability specific to the N-acetyllactosamine (Galβ1-4GlcNAc) site. We determined the genomic and cDNA sequences of the PFA gene, which consists of five exons and spans approximately 5 kb of a genom...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.09.005

    authors： Yokoyama K,Sato M,Haneda T,Yamazaki K,Kitano T,Umetsu K

    更新日期：2014-11-01 00:00:00

  • Rhodnius prolixus supergene families of enzymes potentially associated with insecticide resistance.

    abstract：:Chagas disease or American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, Trypanosoma cruzi. Once known as an endemic health problem of poor rural populations in Latin American countries, it has now spread worldwide. The parasite is transmitted by triatomine bugs, of which...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.06.005

    authors： Schama R,Pedrini N,Juárez MP,Nelson DR,Torres AQ,Valle D,Mesquita RD

    更新日期：2016-02-01 00:00:00

  • Transcription factors, CncC and Maf, regulate expression of CYP6BQ genes responsible for deltamethrin resistance in Tribolium castaneum.

    abstract：:Insecticide resistance is a global problem that presents an ongoing challenge to control insects that destroy crops, trees and transmit diseases. Dramatic progress has been made during the last decade on identification of insecticide resistance-associated genes. In one of the most common resistance mechanisms, insects...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.08.002

    authors： Kalsi M,Palli SR

    更新日期：2015-10-01 00:00:00

  • Male jeffrey pine beetle, Dendroctonus jeffreyi, synthesizes the pheromone component frontalin in anterior midgut tissue.

    abstract：:The male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins (Coleoptera: Scolytidae), produces the bicyclic ketal frontalin as part of a complex semiochemical blend. A key regulated enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), showed high transcript levels in the anterior midgut ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00073-5

    authors： Hall GM,Tittiger C,Blomquist GJ,Andrews GL,Mastick GS,Barkawi LS,Bengoa C,Seybold SJ

    更新日期：2002-11-01 00:00:00

  • Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti.

    abstract：:A blood meal induces the ovaries of female Aedes aegypti mosquitoes to produce ecdysteroid hormones that regulate many processes required for egg maturation. Various proteins involved in the intracellular transport and biosynthesis of ecdysteroid precursors have been identified by analysis of Drosophila melanogaster m...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.01.011

    authors： Sieglaff DH,Duncan KA,Brown MR

    更新日期：2005-05-01 00:00:00

  • Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis.

    abstract：:The insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years in eastern countries. Our previous work has identified a bi-functional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating act...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.04.001

    authors： Wang Y,Yan H,Wang Y,Yang H,Wei L,Xiao Y,Ye H,Lai R,Liu R

    更新日期：2012-08-01 00:00:00

  • A lepidopteran pacifastin member: cloning, gene structure, recombinant production, transcript profiling and in vitro activity.

    abstract：:Members of the pacifastin family have been characterized as serine peptidase inhibitors (PI), but their target enzyme(s) are unknown in insects. So far, the structural and biochemical characteristics of pacifastin-like PI have only been studied in locusts. Here we report the molecular identification and functional cha...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2009.03.005

    authors： Breugelmans B,Simonet G,van Hoef V,Van Soest S,Smagghe G,Vanden Broeck J

    更新日期：2009-07-01 00:00:00

  • Mutations in the Bemisia tabaci para sodium channel gene associated with resistance to a pyrethroid plus organophosphate mixture.

    abstract：:The voltage-gated sodium channel is the primary target site of pyrethroid insecticides. In some insects, super knockdown resistance (super-kdr) to pyrethroids is caused by point mutations in the linker fragment between transmembrane segments 4 and 5 of the para-type sodium channel protein domain II (IIS4-5). Here, we ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00137-6

    authors： Morin S,Williamson MS,Goodson SJ,Brown JK,Tabashnik BE,Dennehy TJ

    更新日期：2002-12-01 00:00:00

  • The silkmoth homolog of the Drosophila ecdysone receptor (B1 isoform): cloning and analysis of expression during follicular cell differentiation.

    abstract：:To understand the role that 20-hydroxy-ecdysone (20E) plays during ovarian development in Bombyx mori, we have undertaken the cloning of the silkworm ecdysone receptor (EcR) and a study of its expression during follicular cell differentiation. We have cloned a cDNA that contains a complete open reading frame for a 68....

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(95)00024-p

    authors： Swevers L,Drevet JR,Lunke MD,Iatrou K

    更新日期：1995-07-01 00:00:00

  • Characterization of the hemoglobin of the backswimmer Anisops deanei (Hemiptera).

    abstract：:While O(2)-binding hemoglobin-like proteins are present in many insects, prominent amounts of hemoglobin have only been found in a few species. Backswimmers of the genera Anisops and Buenoa (Notonectidae) have high concentrations of hemoglobin in the large tracheal cells of the abdomen. Oxygen from the hemoglobin is d...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.04.007

    authors： Wawrowski A,Matthews PG,Gleixner E,Kiger L,Marden MC,Hankeln T,Burmester T

    更新日期：2012-09-01 00:00:00

  • Conservation of a pair of serpin 2 genes and their expression in Amphiesmenoptera.

    abstract：:Silk secreted by the larvae of Hydropsyche angustipennis (Trichoptera) contains serpins HaSerp2A and HaSerp2B that are homologous to serpin 2 known from several lepidopterans and some other insects. The gene HaSerp2A is 2684 bp downstream from the HaSerp2B gene. The genes possess identical exon/intron segmentation (9 ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.01.008

    authors： Yonemura N,Sehnal F,Konik P,Ajimura M,Tamura T,Mita K

    更新日期：2012-05-01 00:00:00

  • Molecular characterization of the VLDL receptor homolog mediating binding of lipophorin in oocyte of the mosquito Aedes aegypti.

    abstract：:Lipophorin (Lp) functions as a yolk protein precursor in the mosquito Aedes aegypti and it is internalized via receptor-mediated endocytosis (Insect Biochem. Mol. Biol., 30 (2000) 1161). We cloned and molecularly characterized a putative mosquito ovarian lipophorin receptor (AaLpRov) cDNA. The cDNA has a length of 346...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00068-6

    authors： Cheon HM,Seo SJ,Sun J,Sappington TW,Raikhel AS

    更新日期：2001-06-22 00:00:00

  • Lipid storage and mobilization in insects: current status and future directions.

    abstract：:In this paper we review the current status of research on fatty acid absorption and conversion to diacylglycerol in the midgut. We further discuss how diacylglycerol may leave the midgut and associate with lipophorin in hemolymph. We review the present understanding of the role of the lipid transfer particle and lipop...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/s0965-1748(00)00102-8

    authors： Arrese EL,Canavoso LE,Jouni ZE,Pennington JE,Tsuchida K,Wells MA

    更新日期：2001-01-01 00:00:00

  • Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori).

    abstract：:NADH-dependent glutamate synthase (Nadh-Gogat; EC 1.41.14) was purified 766-fold from the fat body of 5th instar larvae of the silkworm with a final specific activity of 13.8 units/mg protein by a produce including ammonium sulfate fraction, Q-Sepharose HP ion exchange column chromatography, Blue Sepharose FF affinity...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(98)00019-8

    authors： Hirayama C,Saito H,Konno K,Shinbo H

    更新日期：1998-07-01 00:00:00

  • The E75 gene of Manduca sexta and comparison with its Drosophila homolog.

    abstract：:The ecdysone-inducible E75 gene responsible for the 75B puff of Drosophila melanogaster encodes a family of proteins which are members of the steroid receptor superfamily. These proteins are believed to be involved in the regulation of ecdysone response. In order to investigate the evolutionary conservation of E75, we...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(93)90086-8

    authors： Segraves WA,Woldin C

    更新日期：1993-01-01 00:00:00

  • Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta.

    abstract：:The insect molting hormone 20-hydroxyecdysone (20E) plays a central role in regulating gene expression during development and metamorphosis. In many Lepidoptera, the pro-hormone 3-dehydroecdysone (3DE), synthesized from cholesterol in the prothoracic gland, is rapidly converted to ecdysone (E) by a hemolymph reductase...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.12.002

    authors： Rewitz KF,Rybczynski R,Warren JT,Gilbert LI

    更新日期：2006-03-01 00:00:00

  • Molecular cloning and partial characterization of a trypsin-like protein in salivary glands of Lygus hesperus (Hemiptera: Miridae).

    abstract：:Trypsin-like enzymes from the salivary gland complex (SGC) of Lygus hesperus Knight were partially purified by preparative isoelectric focusing (IEF). Enzyme active against Nalpha-benzoyl-L-arginine-p-nitroanilide (BApNA) focused at approximately pH 10 during IEF. This alkaline fraction gave a single activity band whe...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00123-0

    authors： Zeng F,Zhu YC,Cohen AC

    更新日期：2002-04-01 00:00:00

  • Defense and melanization depend on the eumelanin pathway, occur independently and are controlled differentially in developing Ceratitis capitata.

    abstract：:A defense mechanism in the hemocytes and cuticle of developing Ceratitis capitata has been demonstrated (Marmaras and Charalambidis, 1992; Marmaras et al., 1993a; Marmaras et al., 1993b). To elucidate further the mechanism and the regulation of defense reactions, we studied this process in relation to melanization in ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)90052-3

    authors： Charalambidis ND,Bournazos SN,Lambropoulou M,Marmaras VJ

    更新日期：1994-07-01 00:00:00

  • Inhibition of [3H]ponasterone A binding by ecdysone agonists in the intact Kc cell line.

    abstract：:Inhibition of the binding of [3H]ponasterone A ([3H]PoA) by ecdysone agonists including diacylhydrazines such as RH-5849, tebufenozide (RH-5992) and methoxyfenozide (RH-2485) was examined in intact Drosophila Kc cells. The reciprocal logarithm of the concentration at which there is 50% inhibition of [3H]PoA binding, p...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00105-9

    authors： Nakagawa Y,Minakuchi C,Takahashi K,Ueno T

    更新日期：2002-02-01 00:00:00

  • Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.

    abstract：:Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eseri...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00025-0

    authors： Anazawa Y,Tomita T,Aiki Y,Kozaki T,Kono Y

    更新日期：2003-05-01 00:00:00

  • Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.

    abstract：:A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the ami...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)90057-4

    authors： Rasmussen C,Rohrmann GF

    更新日期：1994-07-01 00:00:00

  • Activation of prophenoloxidase with 2-propanol and other organic compounds in Drosophila melanogaster.

    abstract：:Activation with 2-propanol and other organic compounds of prophenoloxidase purified from pupae of Drosophila melanogaster was analyzed. A1, one of the two isozymes of the prophenoloxidase, could be activated with both an endogenous activating system and artificial organic compounds including alcohols. A1 was activated...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(93)90060-6

    authors： Asada N,Fukumitsu T,Fujimoto K,Masuda K

    更新日期：1993-06-01 00:00:00