Abstract：
:The voltage-gated sodium channel is the primary target site of pyrethroid insecticides. In some insects, super knockdown resistance (super-kdr) to pyrethroids is caused by point mutations in the linker fragment between transmembrane segments 4 and 5 of the para-type sodium channel protein domain II (IIS4-5). Here, we identify two mutations in the IIS4-5 linker of the para-type sodium channel of the whitefly, BEMISIA TABACI: methionine to valine at position 918 (M918V) and leucine to isoleucine at position 925 (L925I). Although each mutation was isolated independently from strains >100-fold resistant to a pyrethroid (fenpropathrin) plus organophosphate (acephate) mixture, only L925I was associated with resistance in strains derived from the field in 2000 and 2001. The L925I mutation occurred in all individuals from nine different field collections that survived exposure to a discriminating concentration of fenpropathrin plus acephate. Linkage analysis of hemizygous male progeny of unmated heterozygous F1 females (L925Ixwild-type) shows that the observed resistance is tightly linked to the voltage-gated sodium channel locus. The results provide a molecular tool for better understanding, monitoring and managing pyrethroid resistance in B. tabaci.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Morin S,Williamson MS,Goodson SJ,Brown JK,Tabashnik BE,Dennehy TJdoi
10.1016/s0965-1748(02)00137-6subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1781-91issue
12eissn
0965-1748issn
1879-0240pii
S0965174802001376journal_volume
32pub_type
杂志文章abstract：:Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene count represents an in...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.09.007
更新日期：2008-01-01 00:00:00
abstract：:The study of hemolymph melanization in Lepidoptera has contributed greatly to our understanding of its role in insect immunity. Manduca sexta in particular has been an excellent model for identifying the myriad components of the phenoloxidase (PO) cascade and their activation through exposure to pathogen-associated mo...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.01.002
更新日期：2015-03-01 00:00:00
abstract：:Injection of low doses of bacteria into the aquatic larvae of the dipteran insect Chironomus plumosus induces the appearance in their hemolymph of a potent antibacterial activity. We have isolated two 36-residue peptides from this hemolymph which are active against Gram-positive bacteria. The peptides are novel member...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(98)00101-5
更新日期：1998-12-01 00:00:00
abstract：:A pheromonotropic peptide was isolated from brain-suboesophageal ganglion complexes of the adult female gypsy moth, Lymantria dispar, using a 5-step HPLC purification protocol and an in vivo bioassay in Helicoverpa zea. The intact peptide was sequenced by automated Edman degradation. The L. dispar pheromone biosynthes...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90111-2
更新日期：1994-09-01 00:00:00
abstract：:Host plasma proteins and protein digestion in larval parasitoids were studied during trophic interactions of the ectoparasitoid Habrobracon hebetor Say (Hymenoptera: Braconidae), with a host, larvae of the Indianmeal moth, Plodia interpunctella Hübner (Lepidoptera: Pyralidae). We could detect no apparent differences i...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00066-7
更新日期：2000-10-01 00:00:00
abstract：:A number of insect ecdysteroids, plant ecdysteroids and juvenoids were assayed for their ability to activate Drosophila nuclear receptors in transfected tissue culture cells. Discrete modifications to 20-hydroxyecdysone, the apparent natural ligand for the ecdysone receptor (EcR), conferred dramatic changes on the tra...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00075-8
更新日期：2000-11-01 00:00:00
abstract：:The medfly genome has been shown to contain a rich assortment of transposable elements from the mariner, Tc1, hAT and gypsy/Ty3 families. These elements display different levels of diversity, abundance and distribution in the genome. The presence of actively transposing elements in the medfly genome is revealed by hyb...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.ibmb.2003.06.015
更新日期：2004-02-01 00:00:00
abstract：:Nicotinic acetylcholine receptors (nAChRs) are targets for insect-selective neonicotinoid insecticides exemplified by imidacloprid (IMI) and mammalian-selective nicotinoids including nicotine and epibatidine (EPI). Despite their importance, insect nAChRs are poorly understood compared with their vertebrate counterpart...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2005.08.006
更新日期：2005-12-01 00:00:00
abstract：:Insects possess a cuticle that covers all tissues exposed to the outside world including the body, the fore- and hindgut and the luminal side of the tracheae. The cuticle is a multifunctional device that protects its carriers against dehydration, arms them against predators, constitutes a physical barrier to prevent p...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.ibmb.2010.03.003
更新日期：2010-05-01 00:00:00
abstract：:Two beta-glycosidases (BG) (Mr 47,000 and Mr 50,000) were purified from Spodoptera frugiperda (Lepidoptera: Noctuidae) midguts. These two polypeptides associate or dissociate depending on the medium ionic strength. The Mr 47,000 BG probably has two active sites. One of the putative active sites (cellobiase site) hydro...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00090-4
更新日期：2000-12-01 00:00:00
abstract：:Cyclorrhapha insect genomes contain a single acetylcholinesterase (AChE) gene while other insects contain at least two ace genes (ace1 and ace2). In this study we tested the hypothesis that the two ace paralogous from Blattella germanica have different contributions to AChE activity, using RNA interference (RNAi) to k...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2009.11.001
更新日期：2009-12-01 00:00:00
abstract：:Messenger RNA levels of the cytochrome P450 gene CYP6A1 were measured in the insecticide resistant Diazinon-R 'Rutgers' strain and in the susceptible strain sbo of the house fly with a cloned cDNA probe. The constitutive expression of the CYP6A1 gene was at least 10 times higher in the Rutgers strain than in the sbo s...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90034-5
更新日期：1994-04-01 00:00:00
abstract：:Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章,评审
doi：10.1016/j.ibmb.2006.01.014
更新日期：2006-04-01 00:00:00
abstract：:The Single von Willebrand factor C-domain proteins (SVCs) are a group of short proteins mainly found in arthropods. They are proposed to be responsive in relation to environmental challenges including the nutritional status, bacterial and viral infections. The SVC protein Vago acts as a cross-talk molecule between the...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.10.002
更新日期：2017-12-01 00:00:00
abstract：:Odorant receptors (Ors) are a unique family of ligand-gated ion channels and the primary mechanism by which insects detect volatile chemicals. Here, we describe 57 putative Ors sequenced from an antennal transcriptome of the cerambycid beetle Megacyllene caryae (Gahan). The male beetles produce a pheromone blend of ni...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.03.007
更新日期：2012-07-01 00:00:00
abstract：:A bioinformatics-based investigation of three insect species with completed genome sequences has revealed that insect chitinase-like proteins (glycosylhydrolase family 18) are encoded by a rather large and diverse group of genes. We identified 16, 16 and 13 putative chitinase-like genes in the genomic databases of the...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2007.06.010
更新日期：2008-04-01 00:00:00
abstract：:A new member of the AKH/RPCH family was isolated and identified from the corpora cardiaca of the firebug Pyrrhocoris apterus. The peptide was isolated in a single step by reversed phase HPLC and the structure deduced from the multiple MS (MS(N)) electrospray mass spectra and amino acid analysis as that of an octapepti...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00025-4
更新日期：2000-06-01 00:00:00
abstract：:Homeobox genes belong to a large gene group, which encodes the famous DNA-binding homeodomain that plays a key role in development and cellular differentiation during embryogenesis in animals. Here, one hundred forty-nine homeobox genes were identified from the Asian scorpion, Mesobuthus martensii (Chelicerata: Arachn...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.04.002
更新日期：2015-06-01 00:00:00
abstract：:A binary mosquitocidal toxin composed of a three-domain Cry-like toxin (Cry48Aa) and a binary-like toxin (Cry49Aa) was identified in Lysinibacillus sphaericus. Cry48Aa/Cry49Aa has action on Culex quinquefasciatus larvae, in particular, to those that are resistant to the Bin Binary toxin, which is the major insecticida...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.08.001
更新日期：2017-09-01 00:00:00
abstract：:Protein digestion in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), results from the action of a complex of serine proteinases present in the midgut. In this study we partially characterized trypsin-like enzyme activity against N-alpha-benzoyl-L-arginine p-nitroanilide (BApNA) in midgut ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00081-8
更新日期：1999-12-01 00:00:00
abstract：:Current knowledge of sand fly salivary components has been based solely on Lutzomyia and Phlebotomus species which feed mainly on mammals; their hyaluronidases and apyrases were demonstrated to significantly affect blood meal intake and transmission of vector-borne pathogens. Members of the third sand fly genus Sergen...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.09.010
更新日期：2018-11-01 00:00:00
abstract：:The importance of Chagas disease motivated the scientific effort to obtain the complete genomic sequence of the vector species Rhodnius prolixus, this information is also relevant to the understanding of triatomine biology in general. The central nervous system is the key regulator of insect physiology and behavior. N...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.05.003
更新日期：2016-02-01 00:00:00
abstract：:Two bisacylhydrazine insecticides with ecdysone-mimetic action, RH-5849 and RH-5992, have been subjected to several bioassay procedures that are prerequisites for ecdysone action in Drosophila larvae: (a) induction of early ecdysone-specific puffs on the polytene chromosomes of the larval salivary glands; (b) secretio...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00077-6
更新日期：1999-11-01 00:00:00
abstract：:Activation with 2-propanol and other organic compounds of prophenoloxidase purified from pupae of Drosophila melanogaster was analyzed. A1, one of the two isozymes of the prophenoloxidase, could be activated with both an endogenous activating system and artificial organic compounds including alcohols. A1 was activated...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(93)90060-6
更新日期：1993-06-01 00:00:00
abstract：:Wood feeding insects depends heavily on the secretion of a combination of cellulases, mainly endoglucanases and other glucanases such as exoglucanases and xylanases, for efficient digestion of the cellulosic materials. To date, although a high number of endoglucanases have been found in xytophagous insects, little is ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2012.05.002
更新日期：2012-09-01 00:00:00
abstract：:There are multiple stages in the life cycle of Plasmodium that invade host cells. Molecular machinery involved is such host-pathogen interactions constitute excellent drug targets and/or vaccine candidates. A screen using a phage display library has previously demonstrated presence of enolase on the surface of the Pla...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.11.004
更新日期：2016-01-01 00:00:00
abstract：:To gain insight into the molecular repertoire of the adult female salivary glands of the tiger mosquito Aedes albopictus, we performed transcriptome and proteome analysis. cDNA clones were sequenced and assembled in clusters of related sequences and the corresponding genes assigned to one of three categories: housekee...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2006.10.007
更新日期：2007-02-01 00:00:00
abstract：:The three-member Egf gene family from the polydnavirus Microplitis demolitor bracovirus (MdBV) encodes novel proteins distinguished by a shared cysteine-rich motif. Prior studies determined that one family member, Egf1.0, inhibits melanization of hemolymph from the moth Manduca sexta by disabling phenoloxidase activat...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2010.04.009
更新日期：2010-07-01 00:00:00
abstract：:Insect exoskeletons are composed of the cuticle, a biomaterial primarily formed from the linear and relatively rigid polysaccharide, chitin, and structural proteins. This extracellular material serves both as a skin and skeleton, protecting insects from environmental stresses and mechanical damage. Despite its rather ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2017.11.003
更新日期：2017-12-01 00:00:00
abstract：:The short days of late summer and early fall are the environmental cues that most temperate insects and other animals use to predict winter's arrival. Although it is still unclear precisely how insects measure daylength, there is mounting evidence that the circadian clock regulates seasonal responses including photope...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2020.103365
更新日期：2020-06-01 00:00:00
