当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Constitutive overexpression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides.

Constitutive overexpression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides.

Abstract：

:Messenger RNA levels of the cytochrome P450 gene CYP6A1 were measured in the insecticide resistant Diazinon-R 'Rutgers' strain and in the susceptible strain sbo of the house fly with a cloned cDNA probe. The constitutive expression of the CYP6A1 gene was at least 10 times higher in the Rutgers strain than in the sbo strain. In both strains, CYP6A1 was inducible by phenobarbital treatment of the flies. Analysis of genomic DNA from the two strains indicated that there was no amplification of the CYP6A1 gene in the Rutgers strain. A developmental analysis revealed that CYP6A1 is expressed larvae and adults, and very low levels of CYP6A1 mRNA were detected in eggs and pupae. The constitutive overexpression of CYP6A1 in the Rutgers strain was observed in both larvae and adults. Crosses between the Rutgers strain and the multiply marked sbo strain revealed that the high constitutive expression of CYP6A1 in the Rutgers strain is controlled by one or more loci located on chromosome II.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Cariño FA,Koener JF,Plapp FW Jr,Feyereisen R

doi

10.1016/0965-1748(94)90034-5

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

411-8

issue

4

eissn

0965-1748

issn

1879-0240

pii

0965-1748(94)90034-5

journal_volume

24

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • In silico approach of azadirachtin binding with actins.

    abstract：:In silico docking analysis reported here suggests that insect cellular cytoskeletal beta-actin could be the target of Azadirachtin A (Aza-the principle bioactive compound of neem seeds). The best docking energy of -40.09 kcal/mol at 8.73 A RMSD and predicted hydrogen bond between Arg210 and carboxymethyl group of Aza ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.03.010

    authors： Pravin Kumar R,Manoj MN,Kush A,Annadurai RS

    更新日期：2007-06-01 00:00:00

  • An insight into the sialome of the frog biting fly, Corethrella appendiculata.

    abstract：:The Nematocera infraorder Culicomorpha is believed to have descended from bloodfeeding ancestors over 200 million years ago, generating bloodfeeding and non-bloodfeeding flies in two superfamilies, the Culicoidea-containing the mosquitoes, the frog-feeding midges, the Chaoboridae, and the Dixidae-and the Chironomoidea...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2013.10.006

    authors： Ribeiro JMC,Chagas AC,Pham VM,Lounibos LP,Calvo E

    更新日期：2014-01-01 00:00:00

  • Silencing of ATG6 and ATG8 promotes increased levels of triacylglycerol (TAG) in the fat body during prolonged starvation periods in the Chagas disease vector Rhodnius prolixus.

    abstract：:Rhodnius prolixus is an obligatorily hematophagous insect known as an important vector of Chagas disease. Autophagy is a conserved cellular mechanism that acts in response to nutrient starvation, where components of the cytoplasm are sequestered by a double membrane organelle, named autophagosome, which is targeted to...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2020.103484

    authors： Santos-Araujo S,Bomfim L,Araripe LO,Bruno R,Ramos I,Gondim KC

    更新日期：2020-12-01 00:00:00

  • Characterization of the hemoglobin of the backswimmer Anisops deanei (Hemiptera).

    abstract：:While O(2)-binding hemoglobin-like proteins are present in many insects, prominent amounts of hemoglobin have only been found in a few species. Backswimmers of the genera Anisops and Buenoa (Notonectidae) have high concentrations of hemoglobin in the large tracheal cells of the abdomen. Oxygen from the hemoglobin is d...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.04.007

    authors： Wawrowski A,Matthews PG,Gleixner E,Kiger L,Marden MC,Hankeln T,Burmester T

    更新日期：2012-09-01 00:00:00

  • Characterization of a chitin synthase cDNA and its increased mRNA level associated with decreased chitin synthesis in Anopheles quadrimaculatus exposed to diflubenzuron.

    abstract：:Chitin synthase (EC 2.4.1.16) is a crucial enzyme responsible for chitin biosynthesis in all chitin-containing organisms. This paper reports a complete cDNA encoding chitin synthase 1 (AqCHS1), change of AqCHS1 mRNA level in response to diflubenzuron exposure, and concentration-dependent effect of diflubenzuron on chi...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.06.002

    authors： Zhang J,Zhu KY

    更新日期：2006-09-01 00:00:00

  • The E75 gene of Manduca sexta and comparison with its Drosophila homolog.

    abstract：:The ecdysone-inducible E75 gene responsible for the 75B puff of Drosophila melanogaster encodes a family of proteins which are members of the steroid receptor superfamily. These proteins are believed to be involved in the regulation of ecdysone response. In order to investigate the evolutionary conservation of E75, we...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(93)90086-8

    authors： Segraves WA,Woldin C

    更新日期：1993-01-01 00:00:00

  • Substrate-stereoselectivity of a high-affinity glutamate transporter cloned from the CNS of the cockroach Diploptera punctata.

    abstract：:A cDNA encoding a Na(+)-dependent glutamate transporter has been cloned from the brain of the cockroach Diploptera punctata. The cDNA encodes a transporter protein of 481 amino acids, designated DipEAAT1, which when expressed in baculovirus infected insect cells, resulted in a 40-50 fold increase in [(3)H]L-glutamate ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00004-7

    authors： Donly C,Jevnikar J,McLean H,Caveney S

    更新日期：2000-05-01 00:00:00

  • Inhibition of cAMP signalling cascade-mediated Ca2+ influx by a prothoracicostatic peptide (Mas-MIP I) via dihydropyridine-sensitive Ca2+ channels in the prothoracic glands of the silkworm, Bombyx mori.

    abstract：:Measurements of Ca(2+) influx in Fura-2/AM loaded prothoracic glands (PGs) of the silkworm, Bombyx mori, after application of forskolin or the cAMP analogue, 8-bromo-cAMP, showed a steady increase in [Ca(2+)](i), which was of extracellular origin and was inhibited, in both cases, by the dihydropyridine (DHP) derivativ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(02)00206-0

    authors： Dedos SG,Birkenbeil H

    更新日期：2003-02-01 00:00:00

  • Armigeres subalbatus prophenoloxidase III: Cloning, characterization and potential role in morphogenesis.

    abstract：:It has long been suggested that phenoloxidases (POs) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning and melanotic encapsulation of pathogens. Here we report that a mosquito PO, designated Armigeres subalbatus prophenoloxidase III (As-pro-PO III)...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.10.007

    authors： Tsao IY,Lin US,Christensen BM,Chen CC

    更新日期：2009-02-01 00:00:00

  • Molecular cloning and partial characterization of a trypsin-like protein in salivary glands of Lygus hesperus (Hemiptera: Miridae).

    abstract：:Trypsin-like enzymes from the salivary gland complex (SGC) of Lygus hesperus Knight were partially purified by preparative isoelectric focusing (IEF). Enzyme active against Nalpha-benzoyl-L-arginine-p-nitroanilide (BApNA) focused at approximately pH 10 during IEF. This alkaline fraction gave a single activity band whe...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00123-0

    authors： Zeng F,Zhu YC,Cohen AC

    更新日期：2002-04-01 00:00:00

  • Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.

    abstract：:Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eseri...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00025-0

    authors： Anazawa Y,Tomita T,Aiki Y,Kozaki T,Kono Y

    更新日期：2003-05-01 00:00:00

  • Dual signalling by different octopamine receptors converges on adenylate cyclase in Sf9 cells.

    abstract：:The Sf9 insect cell line, derived from Spodoptera frugiperda, was used to study the regulation of adenylate cyclase (AC) activity by octopamine receptors. The cyclic AMP (cAMP) production was stimulated in a concentration-dependent manner by octopamine. Octopamine also elicited a rise in cytosolic Ca(2+). The Ca(2+) e...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00089-3

    authors： Näsman J,Kukkonen JP,Akerman KE

    更新日期：2002-03-01 00:00:00

  • Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori.

    abstract：:We have previously cloned and characterized BmJHE, a juvenile hormone (JH)-selective esterase (JHE) that is important for JH titer regulation in the silkworm Bombyx mori. Here, we sought to determine whether multiple genes might function as JH-specific esterase in this species. We searched for putative carboxyl/cholin...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2009.12.015

    authors： Tsubota T,Shimomura M,Ogura T,Seino A,Nakakura T,Mita K,Shinoda T,Shiotsuki T

    更新日期：2010-02-01 00:00:00

  • Structural characteristics of a novel antifreeze protein from the longhorn beetle Rhagium inquisitor.

    abstract：:Antifreeze proteins (AFPs) are characterized by their capacity to inhibit the growth of ice and are produced by a variety of polar fish, terrestrial arthropods and other organisms inhabiting cold environments. This capacity reflects their role as stabilizers of supercooled body fluids. The longhorn beetle Rhagium inqu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.11.002

    authors： Kristiansen E,Ramløv H,Højrup P,Pedersen SA,Hagen L,Zachariassen KE

    更新日期：2011-02-01 00:00:00

  • Stereospecific, mechanism-based, suicide inhibition of a cytochrome P450 involved in ecdysteroid biosynthesis in the prothoracic glands of Manduca sexta.

    abstract：:The first required step in ecdysteroid (molting hormone) biosynthesis, dietary cholesterol (C) conversion to 7-dehydrocholesterol (7dC) via 7,8-dehydrogenation, is mediated by a microsomal cytochrome-P450 monooxygenase specific to the larval prothoracic gland. A subsequent series of unknown "black-box" oxidations of 7...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(95)00007-i

    authors： Warren JT,Rybczynski R,Gilbert LI

    更新日期：1995-06-01 00:00:00

  • Comparative genomics of insect juvenile hormone biosynthesis.

    abstract：:The biosynthesis of insect juvenile hormone (JH) and its neuroendocrine control are attractive targets for chemical control of insect pests and vectors of disease. To facilitate the molecular study of JH biosynthesis, we analyzed ESTs from the glands producing JH, the corpora allata (CA) in the cockroach Diploptera pu...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.01.013

    authors： Noriega FG,Ribeiro JM,Koener JF,Valenzuela JG,Hernandez-Martinez S,Pham VM,Feyereisen R

    更新日期：2006-04-01 00:00:00

  • Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins.

    abstract：:Olfaction in Drosophila is mediated by a large family of membrane-bound odorant receptor proteins (Ors). In heterologous cells, we investigated whether the structural features and signalling mechanisms of ligand-binding Drosophila Ors are consistent with them being G protein-coupled receptors (GPCRs). The detailed mem...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.05.002

    authors： Smart R,Kiely A,Beale M,Vargas E,Carraher C,Kralicek AV,Christie DL,Chen C,Newcomb RD,Warr CG

    更新日期：2008-08-01 00:00:00

  • Programmed cell death of the prothoracic glands of Manduca sexta during pupal-adult metamorphosis.

    abstract：:The degeneration of the prothoracic glands of Manduca sexta during pupal-adult metamorphosis was analyzed by both light and transmission electron microscopy, and correlated with a functional analysis of the ability of the prothoracic glands to synthesize ecdysteroids in vitro. Apoptosis of degenerating glandular cells...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(96)00068-9

    authors： Dai JD,Gilbert LI

    更新日期：1997-01-01 00:00:00

  • A Drosophila male accessory gland protein that is a member of the serpin superfamily of proteinase inhibitors is transferred to females during mating.

    abstract：:Accessory gland peptide 76A, (Acp76A), belongs to the serpin superfamily of proteins (serine protease inhibitors). Acp76A is a secreted protein synthesized only in the Drosophila melanogaster adult male accessory gland. Accumulation of the protein in males is first detected with polyclonal antibodies at 1 day after ec...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)00055-m

    authors： Coleman S,Drähn B,Petersen G,Stolorov J,Kraus K

    更新日期：1995-02-01 00:00:00

  • Functional analysis and localisation of a thyrotropin-releasing hormone-type neuropeptide (EFLa) in hemipteran insects.

    abstract：:EFLamide (EFLa) is a neuropeptide known for a long time from crustaceans, chelicerates and myriapods. Recently, EFLa-encoding genes were identified in the genomes of apterygote hexapods including basal insect species. In pterygote insects, however, evidence of EFLa was limited to partial sequences in the bed bug (Cime...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2020.103376

    authors： Kotwica-Rolinska J,Krištofová L,Chvalová D,Pauchová L,Provazník J,Hejníková M,Sehadová H,Lichý M,Vaněčková H,Doležel D

    更新日期：2020-07-01 00:00:00

  • Characterization of voltage-sensitive sodium channel gene coding sequences from insecticide-susceptible and knockdown-resistant house fly strains.

    abstract：:The kdr insecticide resistance trait of the house fly (Musca domestica .L.), which confers reduced neuronal sensitivity to DDT and pyrethroid insecticides, was previously shown to exhibit tight genetic linkage to restriction fragment length polymorphism markers lying within a voltage-sensitive sodium channel gene that...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(95)00093-3

    authors： Ingles PJ,Adams PM,Knipple DC,Soderlund DM

    更新日期：1996-04-01 00:00:00

  • Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori.

    abstract：:ok mutants of the silkworm, Bombyx mori, exhibit highly translucent larval skin resulting from the inability to incorporate uric acid into the epidermal cells. Here we report the identification of a gene responsible for the ok mutation using positional cloning and RNAi experiments. In two independent ok mutant strains...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2013.03.011

    authors： Wang L,Kiuchi T,Fujii T,Daimon T,Li M,Banno Y,Kikuta S,Kikawada T,Katsuma S,Shimada T

    更新日期：2013-07-01 00:00:00

  • Expression of a sugar clade gustatory receptor, BmGr6, in the oral sensory organs, midgut, and central nervous system of larvae of the silkworm Bombyx mori.

    abstract：:Insects taste nonvolatile chemicals through gustatory receptors (Grs) and make choices for feeding, mating, and oviposition. To date, genome projects have identified 69 Gr genes in the silkworm, Bombyx mori; however, the expression sites of these Grs remain to be explored. In this study, we used reverse transcription ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2015.12.008

    authors： Mang D,Shu M,Endo H,Yoshizawa Y,Nagata S,Kikuta S,Sato R

    更新日期：2016-03-01 00:00:00

  • Glutathione S-transferase in the defence against pyrethroids in insects.

    abstract：:The correlation between the natural levels of GST and the tolerance to the insecticide decamethrin (dMT), as well as the interaction between the molecules of affinity purified enzyme and the insecticide were investigated in order to collect further information on the obscure role of the Glutathione S-transferase syste...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00123-5

    authors： Kostaropoulos I,Papadopoulos AI,Metaxakis A,Boukouvala E,Papadopoulou-Mourkidou E

    更新日期：2001-03-15 00:00:00

  • Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance.

    abstract：:Pyrethroids are the major class of insecticides used for mosquito control. Excessive and improper use of insecticides, however, has resulted in pyrethroid resistance, which has become a major obstacle for mosquito control. The development of pyrethroid resistance is a complex process involving many genes, and informat...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2014.10.007

    authors： Hong S,Guo Q,Wang W,Hu S,Fang F,Lv Y,Yu J,Zou F,Lei Z,Ma K,Ma L,Zhou D,Sun Y,Zhang D,Shen B,Zhu C

    更新日期：2014-12-01 00:00:00

  • Identification, genomic organization and expression pattern of glutathione S-transferase in the silkworm, Bombyx mori.

    abstract：:Glutathione S-transferases (GSTs) are a multifunctional supergene family and some play an important role in insecticide resistance. We have identified 23 putative cytosolic GSTs by searching the new assembly of the Bombyx mori genome sequence. Phylogenetic analyses on the amino acid sequences reveal that 21 of the B. ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.08.002

    authors： Yu Q,Lu C,Li B,Fang S,Zuo W,Dai F,Zhang Z,Xiang Z

    更新日期：2008-12-01 00:00:00

  • Molecular characterization of neuropeptide elevenin and two elevenin receptors, IsElevR1 and IsElevR2, from the blacklegged tick, Ixodes scapularis.

    abstract：:Understanding salivation in hematophagous arthropod vectors is crucial to developing novel methods to prevent vector-borne disease transmission. The interactions between the tick, host, and pathogens during salivation are highly complex, and are dynamically regulated by the tick central nervous system (synganglion). R...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2018.07.005

    authors： Kim D,Šimo L,Park Y

    更新日期：2018-10-01 00:00:00

  • Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.

    abstract：:Targeted mutagenesis is one of the key methods for functional gene analysis. A simplified variant of gene targeting uses direct microinjection of custom-designed Zinc Finger Nuclease (ZFN) mRNAs into Drosophila embryos. To evaluate the applicability of this method to gene targeting in another insect, we mutagenized th...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.07.012

    authors： Takasu Y,Kobayashi I,Beumer K,Uchino K,Sezutsu H,Sajwan S,Carroll D,Tamura T,Zurovec M

    更新日期：2010-10-01 00:00:00

  • Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects.

    abstract：:A bioinformatics-based investigation of three insect species with completed genome sequences has revealed that insect chitinase-like proteins (glycosylhydrolase family 18) are encoded by a rather large and diverse group of genes. We identified 16, 16 and 13 putative chitinase-like genes in the genomic databases of the...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.06.010

    authors： Zhu Q,Arakane Y,Banerjee D,Beeman RW,Kramer KJ,Muthukrishnan S

    更新日期：2008-04-01 00:00:00

  • Histomorphometric and transcriptomic features characterize silk glands' development during the molt to intermolt transition process in silkworm.

    abstract：:The molt-intermolt cycle is an essential feature in holometabolous and hemimetabolous insects' development. In the silkworm, silk glands are under dramatic morphological and functional changes with fibroin genes' transcription being repeatedly turned off and on during the molt-intermolt cycles. However, the molecular ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2016.07.002

    authors： Hu W,Liu C,Cheng T,Li W,Wang N,Xia Q

    更新日期：2016-09-01 00:00:00