当前位置： SCI文献检索 > INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY期刊下所有文献 > Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.

Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite, Tetranychus urticae.

Abstract：

:Acetylcholinesterase (AChE) from two-spotted spider mites, Tetranychus urticae was compared between an organophosphate susceptible (TKD) and a resistant (NCN) strain. The AChE of TKD had lower affinity to acetylthiocholine and propionylthiocholine than that of NCN, and the inhibition of AChE by DDVP, ambenonium, eserine and n-methyl-eserine showed that NCN was more insensitive than TKD. AChE cDNA sequence was determined, and the 687 amino acids of primary structure were deduced. There were six replacements of amino acid residues in TKD and two in NCN. #F331(439)C was the only substitution unique to NCN, however, this mutation existed homozygously in only two out of nine mites. This residue is one of the gorge lining components, and #F331(439)C might act an important role in the sensitivity of AChE to the inhibitors.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Anazawa Y,Tomita T,Aiki Y,Kozaki T,Kono Y

doi

10.1016/s0965-1748(03)00025-0

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

509-14

issue

5

eissn

0965-1748

issn

1879-0240

pii

S0965174803000250

journal_volume

33

pub_type

杂志文章

相关文献

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY文献大全
  • Transcriptional activation of the Drosophila ecdysone receptor by insect and plant ecdysteroids.

    abstract：:A number of insect ecdysteroids, plant ecdysteroids and juvenoids were assayed for their ability to activate Drosophila nuclear receptors in transfected tissue culture cells. Discrete modifications to 20-hydroxyecdysone, the apparent natural ligand for the ecdysone receptor (EcR), conferred dramatic changes on the tra...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00075-8

    authors： Baker KD,Warren JT,Thummel CS,Gilbert LI,Mangelsdorf DJ

    更新日期：2000-11-01 00:00:00

  • A water-specific aquaporin involved in aphid osmoregulation.

    abstract：:The osmotic pressure of plant phloem sap is generally higher than that of insect body fluids. Water cycling from the distal to proximal regions of the gut is believed to contribute to the osmoregulation of aphids and other phloem-feeding insects, with the high flux of water mediated by a membrane-associated aquaporin....

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.08.008

    authors： Shakesby AJ,Wallace IS,Isaacs HV,Pritchard J,Roberts DM,Douglas AE

    更新日期：2009-01-01 00:00:00

  • Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction.

    abstract：:The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.05.009

    authors： Le Goff G,Hilliou F,Siegfried BD,Boundy S,Wajnberg E,Sofer L,Audant P,ffrench-Constant RH,Feyereisen R

    更新日期：2006-08-01 00:00:00

  • Substrate specificity of insect trypsins and the role of their subsites in catalysis.

    abstract：:Trypsins have high sequence similarity, although the responses of insect trypsins to chemical and natural inhibitors suggest they differ in specificities. Purified digestive trypsins from insects of four different orders were assayed with internally quenched fluorescent oligopeptides with two different amino acids at ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.11.006

    authors： Lopes AR,Juliano MA,Marana SR,Juliano L,Terra WR

    更新日期：2006-02-01 00:00:00

  • A lepidopteran pacifastin member: cloning, gene structure, recombinant production, transcript profiling and in vitro activity.

    abstract：:Members of the pacifastin family have been characterized as serine peptidase inhibitors (PI), but their target enzyme(s) are unknown in insects. So far, the structural and biochemical characteristics of pacifastin-like PI have only been studied in locusts. Here we report the molecular identification and functional cha...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2009.03.005

    authors： Breugelmans B,Simonet G,van Hoef V,Van Soest S,Smagghe G,Vanden Broeck J

    更新日期：2009-07-01 00:00:00

  • Identification of surface molecules on salivary glands of the mosquito, Aedes aegypti, by a panel of monoclonal antibodies.

    abstract：:Malaria transmission by the mosquito vector requires sporozoite invasion into mosquito salivary glands. Parasites probably enter the glands by specific receptor-ligand interactions with molecules on the surface of the glands. We have undertaken the characterization of salivary gland surface molecules of Aedes aegypti ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(99)00025-9

    authors： Barreau C,Conrad J,Fischer E,Lujan HD,Vernick KD

    更新日期：1999-06-01 00:00:00

  • Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.

    abstract：:A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the ami...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(94)90057-4

    authors： Rasmussen C,Rohrmann GF

    更新日期：1994-07-01 00:00:00

  • The control of BhB10-1 gene expression in the salivary gland of Bradysia hygida (Diptera, Sciaridae) is disrupted in vivo by a delayed effect of cycloheximide.

    abstract：:BhB10-1 is an amplified gene present in DNA puff B10. This gene is very active in the salivary gland regions S1 and S3 at the end of the larval development. Two transcripts of this gene, 1.3 and 1.1 kb in size, were detected. A secretory protein, SP23, is the product of BhB10-1. In this work, we present evidence suppo...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00156-4

    authors： Conacci ME,Coelho PS,Valente V,de F Sousa J,Paçó-Larson ML,de Almeida JC

    更新日期：2002-07-01 00:00:00

  • Characterization of cuticular proteins in the red flour beetle, Tribolium castaneum.

    abstract：:We are characterizing the cuticular proteins of Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae) to determine their role in the function of the exoskeleton. Based on qualitative analyses of cuticles, we focused on the sodium dodecyl sulfate (SDS)-extractable proteins. A small-scale cuticle "mini-prep" procedure...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(99)00096-x

    authors： Missios S,Davidson HC,Linder D,Mortimer L,Okobi AO,Doctor JS

    更新日期：2000-01-01 00:00:00

  • What the loss of the hormone neuroparsin in the melanogaster subgroup of Drosophila can tell us about its function.

    abstract：:The twelve sequenced Drosophila genomes show the vast majority of neurohormone and neuropeptide genes to be very well conserved. Nonetheless, the gene encoding the hormone neuroparsin has undergone significant evolution. Although Drosophila melanogaster has one of the best known genomes, no neuroparsin gene can be det...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.03.001

    authors： Veenstra JA

    更新日期：2010-04-01 00:00:00

  • Molecular cloning of trypsin-like cDNAs and comparison of proteinase activities in the salivary glands and gut of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae).

    abstract：:Using specific proteinase inhibitors, we demonstrated that serine proteinases in the tarnished plant bug, Lygus lineolaris, are major proteinases in both salivary glands and gut tissues. Gut proteinases were less sensitive to inhibition than proteinases from the salivary glands. Up to 80% azocaseinase and 90% of BApNA...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00094-8

    authors： Zhu YC,Zeng F,Oppert B

    更新日期：2003-09-01 00:00:00

  • The influence of tick behavior, biotope and host specificity on concerted evolution of the platelet aggregation inhibitor savignygrin, from the soft tick Ornithodoros savignyi.

    abstract：:Ticks are obligate blood-feeding parasites that secrete anti-hemostatic components during feeding to enable control of the hemostatic system of the host. Complex interactions at the tick-host interface are an indication of the important role that the host played during tick evolution. The question is to what extent in...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(03)00047-x

    authors： Mans BJ,Louw AI,Neitz AW

    更新日期：2003-06-01 00:00:00

  • Genomic analysis of detoxification genes in the mosquito Aedes aegypti.

    abstract：:Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene count represents an in...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2007.09.007

    authors： Strode C,Wondji CS,David JP,Hawkes NJ,Lumjuan N,Nelson DR,Drane DR,Karunaratne SH,Hemingway J,Black WC 4th,Ranson H

    更新日期：2008-01-01 00:00:00

  • Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis.

    abstract：:Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in Manduca sexta. In this paper w...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/0965-1748(95)00077-1

    authors： Mészáros M,Morton DB

    更新日期：1996-01-01 00:00:00

  • Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.

    abstract：:Engineered nucleases are proteins that are able to cleave DNA at specified sites in the genome. These proteins have recently been used for gene targeting in a number of organisms. We showed earlier that zinc finger nucleases (ZFNs) can be used for generating gene-specific mutations in Bombyx mori by an error-prone DNA...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.10.011

    authors： Sajwan S,Takasu Y,Tamura T,Uchino K,Sezutsu H,Zurovec M

    更新日期：2013-01-01 00:00:00

  • Characterization of the hemoglobin of the backswimmer Anisops deanei (Hemiptera).

    abstract：:While O(2)-binding hemoglobin-like proteins are present in many insects, prominent amounts of hemoglobin have only been found in a few species. Backswimmers of the genera Anisops and Buenoa (Notonectidae) have high concentrations of hemoglobin in the large tracheal cells of the abdomen. Oxygen from the hemoglobin is d...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.04.007

    authors： Wawrowski A,Matthews PG,Gleixner E,Kiger L,Marden MC,Hankeln T,Burmester T

    更新日期：2012-09-01 00:00:00

  • Vasopressin-like peptide and its receptor function in an indirect diuretic signaling pathway in the red flour beetle.

    abstract：:The insect arginine vasopressin-like (AVPL) peptide is of special interest because of its potential function in the regulation of diuresis. Genome sequences of the red flour beetle Tribolium castaneum yielded the genes encoding AVPL and AVPL receptor, whereas the homologous sequences are absent in the genomes of the f...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2008.04.006

    authors： Aikins MJ,Schooley DA,Begum K,Detheux M,Beeman RW,Park Y

    更新日期：2008-07-01 00:00:00

  • Development and ultrastructure of the rigid dorsal and flexible ventral cuticles of the elytron of the red flour beetle, Tribolium castaneum.

    abstract：:Insect exoskeletons are composed of the cuticle, a biomaterial primarily formed from the linear and relatively rigid polysaccharide, chitin, and structural proteins. This extracellular material serves both as a skin and skeleton, protecting insects from environmental stresses and mechanical damage. Despite its rather ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2017.11.003

    authors： Noh MY,Muthukrishnan S,Kramer KJ,Arakane Y

    更新日期：2017-12-01 00:00:00

  • Efficient germ-line transformation of the economically important pest species Lucilia cuprina and Lucilia sericata (Diptera, Calliphoridae).

    abstract：:The green blowfly species Lucilia cuprina and Lucilia sericata are economically important pests for the sheep industries of Australia and New Zealand. L. cuprina has long been considered a good target for a genetic pest management program. In addition, L. sericata maggots are used in the cleaning of wounds and necroti...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2010.09.006

    authors： Concha C,Belikoff EJ,Carey BL,Li F,Schiemann AH,Scott MJ

    更新日期：2011-01-01 00:00:00

  • Dual signalling by different octopamine receptors converges on adenylate cyclase in Sf9 cells.

    abstract：:The Sf9 insect cell line, derived from Spodoptera frugiperda, was used to study the regulation of adenylate cyclase (AC) activity by octopamine receptors. The cyclic AMP (cAMP) production was stimulated in a concentration-dependent manner by octopamine. Octopamine also elicited a rise in cytosolic Ca(2+). The Ca(2+) e...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(01)00089-3

    authors： Näsman J,Kukkonen JP,Akerman KE

    更新日期：2002-03-01 00:00:00

  • Proteomics and transcriptome analysis coupled with pharmacological test reveals the diversity of anti-thrombosis proteins from the medicinal insect, Eupolyphaga sinensis.

    abstract：:The insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years in eastern countries. Our previous work has identified a bi-functional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating act...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.04.001

    authors： Wang Y,Yan H,Wang Y,Yang H,Wei L,Xiao Y,Ye H,Lai R,Liu R

    更新日期：2012-08-01 00:00:00

  • Cloning, expression and characterization of lepidopteran isopentenyl diphosphate isomerase.

    abstract：:Isopentenyl diphosphate isomerase (IPPI) of the spruce budworm, Choristoneura fumiferana, and of the tobacco hornworm, Manduca sexta, was cloned and its catalytic properties assessed. In the presence of Mg(2+) or Mn(2+), the recombinant protein from C. fumiferana (CfIPPI) efficiently isomerized IPP to dimethylallyl di...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2012.07.001

    authors： Sen SE,Tomasello A,Grasso M,Denton R,Macor J,Béliveau C,Cusson M,Crowell DN

    更新日期：2012-10-01 00:00:00

  • The structure of the Drosophila melanogaster sex peptide: Identification of hydroxylated isoleucine and a strain variation in the pattern of amino acid hydroxylation.

    abstract：:In Drosophila melanogaster mating triggers profound changes in the behaviour and reproductive physiology of the female. Many of these post-mating effects are elicited by sex peptide (SP), a 36-mer pheromone made in the male accessory gland and passed to the female in the seminal fluid. The peptide comprises several st...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2020.103414

    authors： Sturm S,Dowle A,Audsley N,Isaac RE

    更新日期：2020-09-01 00:00:00

  • Characterization of midgut trypsin-like enzymes and three trypsinogen cDNAs from the lesser grain borer, Rhyzopertha dominica (Coleoptera: Bostrichidae).

    abstract：:Protein digestion in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), results from the action of a complex of serine proteinases present in the midgut. In this study we partially characterized trypsin-like enzyme activity against N-alpha-benzoyl-L-arginine p-nitroanilide (BApNA) in midgut ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(99)00081-8

    authors： Zhu YC,Baker JE

    更新日期：1999-12-01 00:00:00

  • Sequence characterization and expression patterns of defensin and lysozyme encoding genes from the gut of the reduviid bug Triatoma brasiliensis.

    abstract：:The cDNAs encoding an intestinal defensin (def1) and lysozyme (lys1) of the reduviid bug Triatoma brasiliensis have been amplified by PCR using specific oligonucleotide primers and 5'- and 3'-RACE, cloned and sequenced. The 576 bp clone has an open reading frame of 282 bp and encodes a pre-prodefensin with 94 amino ac...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2006.04.003

    authors： Araújo CA,Waniek PJ,Stock P,Mayer C,Jansen AM,Schaub GA

    更新日期：2006-07-01 00:00:00

  • Molecular basis of Colorado potato beetle adaptation to potato plant defence at the level of digestive cysteine proteinases.

    abstract：:Potato synthesises high levels of proteinase inhibitors in response to insect attack. This can adversely affect protein digestion in the insects, leading to reduced growth, delayed development and lowered fecundity. Colorado potato beetle overcomes this defence mechanism by changing the composition of its digestive pr...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2004.01.003

    authors： Gruden K,Kuipers AG,Guncar G,Slapar N,Strukelj B,Jongsma MA

    更新日期：2004-04-01 00:00:00

  • Identification and differential expression of a sex-peptide receptor in Helicoverpa armigera.

    abstract：:Sex-pheromone production in the night flying female moth, Helicoverpa armigera is under neuroendocrine control due to the timely release of Pheromone Biosynthesis-Activating Neuropeptide (PBAN). Males orient to the females by upwind anemotaxis which usually leads to a successful mating. During copulation insect males ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2011.03.004

    authors： Hanin O,Azrielli A,Zakin V,Applebaum S,Rafaeli A

    更新日期：2011-08-01 00:00:00

  • Molecular cloning and comparative analysis of fibrinogen-related proteins from the soft tick Ornithodoros moubata and the hard tick Ixodes ricinus.

    abstract：:Among disease-vectors, the evolution of the tick innate immune system is still lagging when compared to insects. Such an investigation, which was initiated, by first cloning and sequencing lectins associated in the innate immunity of invertebrates and having fibrinogen related domains, helped in the sequencing of cDNA...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2005.04.001

    authors： Rego RO,Hajdusek O,Kovár V,Kopácek P,Grubhoffer L,Hypsa V

    更新日期：2005-09-01 00:00:00

  • Changes in microRNA abundance may regulate diapause in the flesh fly, Sarcophaga bullata.

    abstract：:Diapause, an alternative developmental pathway characterized by changes in developmental timing and metabolism, is coordinated by molecular mechanisms that are not completely understood. MicroRNA (miRNA) mediated gene silencing is emerging as a key component of animal development and may have a significant role in ini...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.ibmb.2017.03.002

    authors： Reynolds JA,Peyton JT,Denlinger DL

    更新日期：2017-05-01 00:00:00

  • A genomic clone for a chitinase gene from the silkworm, Bombyx mori: structural organization identifies functional motifs.

    abstract：:A genomic clone containing the chitinase gene was isolated and characterized from the silkworm, Bombyx mori, using the polymerase chain reaction technique directed by primers designed from the chitinase cDNA of the insect previously cloned [Insect Biochem. Molec. Biol. 28 (1998) 163]. Results from nucleotide sequence ...

    journal_title：Insect biochemistry and molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0965-1748(00)00160-0

    authors： Abdel-Banat BM,Koga D

    更新日期：2001-03-15 00:00:00