Abstract:
:Cell migration is essential to cancer invasion and metastasis and is spatially and temporally integrated through transcriptionally dependent and independent mechanisms. As cell migration is studied in vitro, it is important to identify genes that both drive cell migration and are biologically relevant in promoting invasion and metastasis in patients with cancer. Here, gene expression profiling and a high-throughput cell migration system answers this question in human bladder cancer. In vitro migration rates of 40 microarray-profiled human bladder cancer cell lines were measured by radial migration assay. Genes whose expression was either directly or inversely associated with cell migration rate were identified and subsequently evaluated for their association with cancer stage in 61 patients. This analysis identified genes known to be associated with cell invasion such as versican, and novel ones, including metallothionein 1E (MT1E) and nicotinamide N-methyltransferase (NNMT), whose expression correlated positively with cancer cell migration and tumor stage. Using loss of function analysis, we show that MT1E and NNMT are necessary for cancer cell migration. These studies provide a general approach to identify the clinically relevant genes in cancer cell migration and mechanistically implicate two novel genes in this process in human bladder cancer.
journal_name
Oncogenejournal_title
Oncogeneauthors
Wu Y,Siadaty MS,Berens ME,Hampton GM,Theodorescu Ddoi
10.1038/onc.2008.264subject
Has Abstractpub_date
2008-11-06 00:00:00pages
6679-89issue
52eissn
0950-9232issn
1476-5594pii
onc2008264journal_volume
27pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::AMID, also called PRG3, is an AIF-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. In this report, we demonstrated that human AMID gene promoter was activated by p53 in reporter gene assays. Chromatin immunoprecipitation experiments indicated that p53 could bind...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207909
更新日期:2004-09-02 00:00:00
abstract::Mitochondria have been classically characterized as organelles with responsibility for cellular energy production in the form of ATP, but they are also the organelles through which apoptotic signaling occurs. Cell stress stimuli can result in outer membrane permeabilization, after which mitochondria release numerous p...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2012.348
更新日期:2013-05-30 00:00:00
abstract::Transcriptional silencing of antitumor genes via CpG island methylation could be a mechanism mediating prostate cancer (PCa) progression from an androgen-sensitive (AS) to an androgen-insensitive (AI) state. We have used the methylation-sensitive restriction fingerprinting (MSRF) technique to identify novel CpG-rich s...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207076
更新日期:2004-01-08 00:00:00
abstract::Many components of the Wnt/β-catenin signaling pathway have critical functions in mammary gland development and tumor formation, yet the contribution of glycogen synthase kinase-3 (GSK-3α and GSK-3β) to mammopoiesis and oncogenesis is unclear. Here, we report that WAP-Cre-mediated deletion of GSK-3 in the mammary epit...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.279
更新日期:2015-07-01 00:00:00
abstract::The MLL (HRX/ALL-1 gene is frequently disrupted in infantile leukemias and therapy-related leukemias and fused to various translocation partner genes. We previously showed that chimeric MLL proteins localize in the nuclei in a fashion similar to that of MLL protein even if the partner gene encodes a cytoplasmic protei...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202400
更新日期:1999-01-28 00:00:00
abstract::Human xeroderma pigmentosum (XP) fibroblasts were transformed with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The transformed cells, called ASKMN, were immortalized, grew in agar and were tumorigenic in nude mice. A trp-met oncogene was identified in ASKMN cells, after transfection of high molecular weight DNA on 3T...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Previous reports have indicated that the cellular slime mold Dictyostelium discoideum possesses two ras genes (rasG and rasD) and one rap gene (rap1). All three genes are developmentally regulated, with each showing a different pattern of transcription during the Dictyostelium life cycle. To establish whether there ar...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::Hox genes encode DNA-binding proteins that are deployed in overlapping domains along various body axes during embryonic development. This sequential activation of Hox genes in temporal and spatial mode, the Hox code, is critical for the proper positioning of segmented structures along those axes, which include the ver...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.484
更新日期:2011-01-27 00:00:00
abstract::E2F transcription factors are important regulators of the cell cycle, and unrestrained activation of E2F-dependent transcription is considered to be an important driver of tumor formation and progression. Although highly expressed in normal skin and skin cancer, the role of the atypical E2Fs, E2F7 and E2F8, in keratin...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.251
更新日期:2017-02-09 00:00:00
abstract::We recently reported an interaction between the p14(ARF) protein and human topoisomerase I (Topo I) resulting in the stimulation of the relaxation activity of Topo I. Our data showed that the complex between the two proteins was located within the nucleolus. In the present work, we have investigated the regions of p14...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206214
更新日期:2003-04-03 00:00:00
abstract::Tumor suppressor gene CDKN2 (also called MTS1, CDK4I and p16INK4) is located in 9p21 and deleted homozygously in a high percentage of tumor cell lines. We have examined the sequence of CDKN2 in 154 tumor cell lines that are not homozygously deleted for CDKN2. Overall, 18% (27/154) of the cell lines carried mutations i...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-03-16 00:00:00
abstract::P16 was originally discovered by its ability to interact with CDK4 and to specifically inhibit the catalytic activity of the CDK4/D1 kinase. Increased attention has focused on the p16 gene because of its location on chromosome 9p21, a region involved in chromosomal rearrangements in a large number of tumor types. The ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-16 00:00:00
abstract::The product of the RB susceptibility gene has been shown to be differentially phosphorylated during the cell cycle, suggesting a role in the regulation of cell cycle progression. We examined the expression and phosphorylation status of the RB protein in senescent Syrian hamster embryo cells. Both phosphorylated and un...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::A new class of nontransformed revertant cells has been isolated from the ras-transformed cell line DT using cis-4-hydroxy-L-proline (CHP) as a selective agent. The new revertants, CHP 9CJ and CHP CB4, each contain two copies of the v-Ki-ras gene, elevated levels of phosphorylated p21ras protein, and rescuable transfor...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Despite the fact that objective response rates to 5-FU are as low as 20%, 5-FU remains the most commonly used drug for the treatment of colorectal cancer. The lack of understanding of resistance to 5-FU, therefore, remains a significant impediment in maximizing its efficacy. We used intestinal epithelial cells with an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208552
更新日期:2005-06-02 00:00:00
abstract::The mouse int6 gene is a frequent integration site of the mouse mammary tumor virus and INT6 silencing by RNA interference in HeLa cells causes an increased number of cells in the G2/M phases of the cell cycle, along with mitotic defects. In this report, we investigated the functional significance of the interaction b...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210314
更新日期:2007-08-02 00:00:00
abstract::We have previously shown (Smith et al., 1994) that antibodies raised against the growth arrest and DNA damage inducible protein Gadd45 co-precipitate proliferating cell nuclear antigen (PCNA), a protein involved in DNA replication and repair. Here we demonstrate that Gadd45 can directly bind to PCNA using a Far-wester...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-16 00:00:00
abstract::The MAP kinase pathway impinging on ERK2 has been shown to be integrally associated with mitogenic signalling in many cell types. Previously, we and others have demonstrated that oncogenic forms of Raf-1 kinase, when expressed in fibroblasts, lead to the constitutive activation of ERK2, the de-regulation of c-fos expr...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-16 00:00:00
abstract::Overexpression of the receptor tyrosine kinase ERBB2 (also known as HER2) occurs in around 15% of breast cancers and is driven by amplification of the ERBB2 gene. ERBB2 amplification is a marker of poor prognosis, and although anti-ERBB2-targeted therapies have shown significant clinical benefit, de novo and acquired ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.625
更新日期:2014-01-30 00:00:00
abstract::The p53-regulated GADD45 gene is one of the important players in cellular response to DNA damage, and probably involved in the control of cell cycle checkpoint, apoptosis and DNA repair. There are both the p53-dependent and -independent pathways that regulate GADD45 induction. Following ionizing radiation, induction o...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204390
更新日期:2001-05-10 00:00:00
abstract::The protein encoded by the MAX gene is a member of the class of basic region-helix-loop-helix-zipper proteins and has been demonstrated to associate with N-, L-, and c-Myc proteins both in vitro and in vivo. Heterodimers formed between c-Myc and Max proteins have been shown to possess sequence-specific DNA-binding act...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Downregulation of the cellular retinol-binding protein-I (CRBP-I) occurs in breast and other human cancers, but its significance is not well understood. Recently, we showed that restoration of CRBP-I expression in transformed MTSV1-7 breast epithelial cells increased retinoic receptor activity, inhibited anoikis, prom...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208347
更新日期:2005-02-24 00:00:00
abstract::Max is a small helix-loop-helix protein which forms heterodimers with members of the Myc protein family. Myc/Max heterodimers exhibit sequence-specific DNA binding with much greater affinity than Myc homodimers. The Xenopus laevis homologue of Max, XMax, is shorter than the equivalent mammalian protein. This differenc...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Transforming Growth Factor-beta1 (TGF-beta1) inhibits the proliferation of most cells, but stimulates some mesenchymal cell types, including murine NIH3T3 fibroblasts. We show here that TGF-beta1 growth stimulation of NIH3T3 fibroblasts is reversed when these cells are transformed by SV40 or are transfected with a pla...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202341
更新日期:1999-01-21 00:00:00
abstract::BTG2, a p53-inducible antiproliferative gene, is stimulated in breast cancer cells by activation of nuclear factor kappa B (NF-kappaB). In rat mammary glands, BTG2 is expressed in epithelial cells and levels decreased during pregnancy and lactation but recovered during involution. Estrogen and progestin suppress BTG2 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208008
更新日期:2004-10-28 00:00:00
abstract::PIKE-A (phosphoinositide 3-kinases (PI 3)-kinase enhancer) is a ubiquitously expressed GTPase, which binds to and enhances protein kinase B (Akt) kinase activity in a guanine nucleotide-dependent manner. PIKE-A is one of the components of the CDK4 amplicon that is amplified in numerous human cancers. However, whether ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210290
更新日期:2007-07-26 00:00:00
abstract::An essential mode of acquired resistance to radiotherapy (RT) appears to be promotion of tumor cell motility and invasiveness in various cancer types, including glioblastoma, a process resembling 'evasive resistance'. Hence, a logical advancement of RT would be to identify suitable complementary treatment strategies, ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.137
更新日期:2013-02-28 00:00:00
abstract::The RASSF8 gene, which maps close to the KRAS2 gene, contains a RAS-associated domain and encodes a protein that is evolutionarily conserved from fish to humans. Analysis of the RASSF8 transcript revealed a complex expression pattern of 5'-UTR mRNA isoforms in normal lung and in lung adenocarcinomas (ADCAs), with no a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209422
更新日期:2006-06-29 00:00:00
abstract::Hepatitis C virus (HCV) core has a pleiotropic effect on various promoters. In this study, we found that the expression of nucleolar phosphoprotein B23 was enhanced in HCV core-expressing cells and, moreover, HCV core interacts directly with the C-terminal end of B23. Using sucrose gradient centrifugation analysis and...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209052
更新日期:2006-01-19 00:00:00
abstract::Wilms tumours (WTs) have two distinct types of histology with or without ectopic mesenchymal elements, suggesting that WTs arise from either the mesenchymal or epithelial nephrogenic lineages. Regardless of the presence or absence of CTNNB1 mutations, nuclear accumulation of beta-catenin is often observed in WTs with ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.455
更新日期:2009-02-26 00:00:00