Abstract:
:Regulation of the lactogen specific binding sites of rat liver was studied in several different high lactogen states. The specific binding of [125I]iodo-hGH was determined as a measure of these sites. Hypophysectomy of pregnant rats produced a much smaller decrease in hepatic binding of [125I]iodo-hGH than was seen in nonpregnant females. Three different prolactin secreting tumors (MtT/F4, MtT/F45, MtT/W5) produced greatly elevated levels of both rPRL and hepatic binding of [125I]iodo-hGH in both male and female rats. The increased binding reflected an increase in the concentration of lactogen specific membrane binding sites. There was no change in the apparent affinity of binding. Hypophysectomy of tumor-bearing rats was not followed by a decrease in the concentration of hepatic sites. Pituitary transplants beneath the kidney capsules of hypophysectomized (hypox) rats produced elevated rPRL levels and increased [125I]iod-GH specific binding to hepatic membranes. The elevation of serum rPRL preceded the increase in hepatic binding. There was a direct correlation between rPRL levels attained by 8 days after implantation and the level of hepatic binding. Estrogen treatment did not alter this correlation. Hormonal replacement with prolactin in combination with various hormones failed to induce the lactogen specific hepatic sites in estrogen treated hypox males, and did not prevent the marked decrease in hepatic binding observed in females following hypophysectomy. It is suggested that chronic elevation of prolactin plays a role in inducing lactogen specific binding sites in rat liver. Estrogen's inductive action seems to be largely effected at the pituitary level.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Posner BIdoi
10.1210/endo-99-5-1168subject
Has Abstractpub_date
1976-11-01 00:00:00pages
1168-77issue
5eissn
0013-7227issn
1945-7170journal_volume
99pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Control of trabecular bone volume is mediated by local events. Recently, it has been shown that the cytokines interleukin-1 (IL-1), tumor necrosis factor alpha (TNF alpha), and TNF beta [also called lymphotoxin (LT)] produced by immune cells all stimulate osteoclast activity, whereas the lymphokine interferon-gamma (I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2494
更新日期:1987-06-01 00:00:00
abstract::Obesity and its associated comorbidities are of major worldwide concern. It is now recognized that there are a number of metabolically distinct pathways of obesity development. The present paper investigates the effect of moderate daily exercise on the underlying mechanisms of one such pathway to obesity, through inte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1035
更新日期:2009-01-01 00:00:00
abstract::The development of acquired resistance to antihormonal agents in breast cancer is a major therapeutic problem. We have developed a tamoxifen-resistant (TAM-R) MCF-7 breast cancer cell line to investigate the mechanisms behind this condition. Both epidermal growth factor receptor (EGFR) and c-erbB2 mRNA and protein exp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220620
更新日期:2003-03-01 00:00:00
abstract::Cloning of GnRH receptors from several animal species has made it possible to investigate receptor function using site-directed mutagenesis. However, many mutant GnRH receptors exhibit decreased ligand binding, which makes analysis of their ligand binding characteristics technically difficult. To increase the affinity...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.10.6260
更新日期:1998-10-01 00:00:00
abstract::Gene expression of fibroblast growth factor-9 (FGF9) is decreased in granulosa cells (GC) of cystic follicles compared with normal dominant follicles in cattle. The objectives of this study were to investigate the effects of FGF9 on GC steroidogenesis, gene expression, and cell proliferation and to determine the hormo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1003
更新日期:2012-09-01 00:00:00
abstract::Covalent adduction of a nitrosyl group to cysteines [S-nitrosylation (S-NO)] is emerging as a key route for nitric oxide (NO) to directly modulate protein functions. Here, we studied the effects of estrogens on endothelial protein S-NO and analyzed the nitrosyl-proteomes by biotin/CyDye switch technique combined with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1356
更新日期:2010-08-01 00:00:00
abstract::Thyroid hormones are essential for normal development and metabolism. Their synthesis requires transport of iodide into thyroid follicles. The mechanisms involving the apical efflux of iodide into the follicular lumen are poorly elucidated. The discovery of mutations in the SLC26A4 gene in patients with Pendred syndro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1548
更新日期:2012-01-01 00:00:00
abstract::In preovulatory ovarian follicles, the oocyte is maintained in meiotic prophase arrest by natriuretic peptide precursor C (NPPC) and its receptor natriuretic peptide receptor 2 (NPR2). LH treatment results in the decrease of NPR2 guanylyl cyclase activity that promotes resumption of meiosis. We investigated the regula...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1133
更新日期:2013-09-01 00:00:00
abstract::An assay was developed to detect tritium-labeled ovine FSH beta-subunit [( 3H]oFSH beta) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSH beta (RCM-oFSH beta) in a two-cycle immunoextraction procedure. A discrete species...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-154
更新日期:1984-07-01 00:00:00
abstract::A member of the interferon-inducible p200 family of proteins, p204, has recently been reported to function in the development of many mesoderm-derived tissues, such as bone, muscle, and cartilage. However, no published study has yet investigated the role of p204 in adipogenesis. Our preliminary experiments showed that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1381
更新日期:2010-07-01 00:00:00
abstract::The influence of GH and several other pituitary hormones on the secretion and intracellular content of immunoreactive somatostatin (IRS) in cultured hypothalamic cells was examined. Hypothalamic cells prepared from 17-day-old rat embryos and maintained as reaggregate monolayers for 12 days in vitro were used. Short te...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-538
更新日期:1985-08-01 00:00:00
abstract::The FOXO family of forkhead transcription factors stimulates the transcription of target genes involved in many fundamental cell processes, including cell survival, cell cycle progression, DNA repair, and insulin sensitivity. The activity of FOXO proteins is principally regulated by activation of protein kinase B (PKB...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0481
更新日期:2003-12-01 00:00:00
abstract::Male and female Holtzman rats were raised from weaning on a vitamin D-deficient diet (-D). Control (+D) animals were raised on the same diet and orally given 70 IU vitamin D3 twice each week. Serum Ca levels fell at the same rate in -D males and females. In contrast, serum parathyroid hormone increased more rapidly in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-5-1528
更新日期:1981-11-01 00:00:00
abstract::Parathyroid tissue from patients with hyperparathyroidism (HPT) exhibited reduced immunohistochemical reactivity with monoclonal antiparathyroid antibodies, previously shown to stain intensely the surface of normal human parathyroid cells and to interfere with a receptor mechanism of these cells which is involved in t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2999
更新日期:1988-06-01 00:00:00
abstract::The influence of administration of ovine prolactin in vivo on intestinal fluid and ion transport in vitro was investigated using intact and hypophysectomized male rats. Prolactin administration significantly stimulated fluid, sodium,potassium, calcium, magnesium and chloride transport across everted jejunal sacs. The ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-5-1158
更新日期:1975-05-01 00:00:00
abstract::Involvement of ionized cytosolic calcium ([Ca2+]i) and protein kinase-C (PKC) in GnRH-stimulated LH release was assessed by correlating measurable changes in [Ca2+]i and LH release in PKC-depleted and nondepleted gonadotropes. Primary cultures of anterior pituitary cells were loaded with the calcium-sensitive fluoresc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.4.1547730
更新日期:1992-04-01 00:00:00
abstract::Oxytocin stimulates phosphoinositide turnover in myometrium. To elucidate whether the coupling mechanism involves the interaction of oxytocin receptor with GTP-binding proteins, we examined oxytocin stimulation of guanosine triphosphatase (GTPase) activity and phospholipase-C activity in rat and human myometrial membr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.4.7895660
更新日期:1995-04-01 00:00:00
abstract::GH promotes the conversion of cultured preadipose 3T3 cells into adipose cells. The serum of most animals also promotes this differentiation. In order to determine the extent to which GH is responsible for the adipogenic activity of serum, we used a specific antiserum able to suppress completely the adipogenic activit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-2-527
更新日期:1984-02-01 00:00:00
abstract::It is established that androgens and unidentified Sertoli cell (SC)-derived factors can influence the development of adult Leydig cells (LC) in rodents, but the mechanisms are unclear. We evaluated adult LC development and function in SC-selective androgen receptor (AR) knockout (SCARKO) and complete AR knockout (ARKO...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0300
更新日期:2005-09-01 00:00:00
abstract::Depletion of hypothalamic norepinephrine (NE) and epinephrine by administration of diethyldithiocarbamate abolished the stimulatory effects of intraventricular (IVT) angiotensin II (AII) on LH release in ovariectomized rats pretreated with estradiol and progesterone. The increase in blood LH produced by IVT NE or iv L...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-6-2728
更新日期:1986-12-01 00:00:00
abstract::Antisera to porcine relaxin were produced in rabbits injected with different fractions that had been separated by polyacrylamide gel electrophoresis (PAGE). Analyses by agar double immunodiffusion demonstrated that the different fractions of relaxin separated by PAGE have similar antigenic sites and the individual fra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-3-679
更新日期:1977-09-01 00:00:00
abstract::Adrenal secretory rates of catecholamines in response to a repeated stimulus have not been described. We have used repeated small hemorrhages spaced 24 h apart in pentobarbital anesthetized dogs prepared with a chronic adrenal venous catheter to study this question. Our results confirm earlier reports that an initial ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-6-1917
更新日期:1982-12-01 00:00:00
abstract::Incubation of bovine corpus luteum slices at 37 C with luteinizing hormone showed that 10.0 microng LH/ml caused a maximum rise in tissue cyclic AMP content within 15 min. Slices incubated with 1.0 or 0.1 microng LH/ml showed a much more gradual accumulation of this nucleotide. In the absence of added LH, a marked dec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-6-1571
更新日期:1977-06-01 00:00:00
abstract::The ability of a monoclonal antiidiotypic antibody (clone 1D5) directed against the binding site of a monoclonal antiestradiol antibody to interact with the estrogen receptor (ER) was investigated. The following lines of evidence indicate that clone 1D5 has the capacity of mimicking the actions of estradiol, and recog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597160
更新日期:1992-06-01 00:00:00
abstract::Studies show that a subset of transgender men desire children; however, there is a paucity of literature on the effect of gender-affirming testosterone therapy on reproductive function. In this manuscript, we will review the process of gender-affirming hormone therapy for transgender men and what is known about ovaria...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqaa014
更新日期:2020-03-01 00:00:00
abstract::We recently reported that aluminum administration to beagles stimulates uncoupled bone formation in the marrow cavity which increases trabecular bone volume and generates new osseous networks within the axial skeleton. To investigate whether this osteogenic process results from direct stimulation of bone cell replicat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-6-3144
更新日期:1991-06-01 00:00:00
abstract::Naturally occurring and synthetic plant flavonoids, such as EMD 21388, are potent inhibitors of thyroid hormone 5'-deiodinase (5'-D) in vitro, but not when given in vivo, since they are tightly bound by serum transthyretin (TTR). EMD 21388 also inhibits the binding of T4 to human, dog, and rat serum TTR in vitro and w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-2890
更新日期:1990-06-01 00:00:00
abstract::Binding of PTH to purified canine renal cortical membranes was investigated using biologically active radioiodinated bovine PTH-(1-84) [bPTH-(1-84)] as radioligand. PTH-(1-84) is thought to be the major circulating form of bioactive PTH, but oxidative inactivation upon iodination has prevented its use as a radioligand...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-4-1303
更新日期:1983-04-01 00:00:00
abstract::Activin A is a gonadal protein originally isolated from follicular fluid and is recognized as a local regulator of granulosa cell differentiation. Whether activin A promotes folliculogenesis, however, still remains unclarified. The present study was designed to elucidate the effect of activin A on follicular growth in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.11.5526
更新日期:1997-11-01 00:00:00
abstract::Although it is evident that androgens increase muscle mass and strength, little is known about the critical molecular targets of androgens in skeletal muscle. In rodents, the skeletal alpha-actin gene is a tissue-specific gene expressed only in the levator ani and other skeletal muscles but not in the prostate or prep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0530
更新日期:2008-03-01 00:00:00