Abstract:
:DNA sequencing was used to confirm Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids in endocervical swab samples. DNA in residues of the samples with positive results by 2 commercial kits was subjected to nested polymerase chain reaction (PCR) amplification. The nested PCR amplicons were used as templates for direct automated DNA sequencing. A 40-base signature sequence was sufficient to achieve unequivocal validation of C trachomatis cryptic plasmid and gonococcal opa gene DNA. DNA with a signature sequence specific for C trachomatis was identified in all 14 samples and for N gonorrhoeae in all 13 samples with positive results by the commercial kits for these 2 microbes. In a low-prevalence population, PCR retesting of 289 samples with initial negative results by a non-nucleic acid amplification assay revealed 3 samples positive for C trachomatis and 2 samples positive for N gonorrhoeae that were missed by the commercial kit. DNA sequencing is a useful tool in validating molecular diagnostics.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Lee SH,Vigliotti VS,Pappu Sdoi
10.1309/Y2202RVCGRWW1RWBsubject
Has Abstractpub_date
2008-06-01 00:00:00pages
852-9issue
6eissn
0002-9173issn
1943-7722pii
G36616512913NHU7journal_volume
129pub_type
杂志文章abstract::The development of sensitive immunofluorescent technics for the detection of platelet antibodies has stimulated renewed interest in the field of platelet immunology. However, the analysis of multispecific sera containing both HLA and platelet-specific antibodies requires cumbersome absorption technics and relatively l...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.6.839
更新日期:1983-12-01 00:00:00
abstract::A case of metastatic ameloblastoma of the maxilla with secondary hypercalcemia in a 54-year-old man is presented. After treatment with surgery, chemotherapy, and radiotherapy, the patient was found to have multiple metastases and severe hypercalcemia associated with high levels of parathormone-like substance in the pe...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/75.4.585
更新日期:1981-04-01 00:00:00
abstract::We describe 41 cases of myeloid neoplasms (MNs) secondary to plasma cell myeloma (PCM). The types of MN included myelodysplastic syndrome (MDS) in 34 (82.9%), acute myeloid leukemia (AML) in 4 (9.8%), and myeloproliferative neoplasm (MPN) or MDS/MPN in 3 (7.3%) cases. The latency from treatment to diagnosis of MN rang...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1309/AJCPOP7APGDT9JIU
更新日期:2012-12-01 00:00:00
abstract::We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4QUN-J4F2-7QK7-RR0G
更新日期:2004-11-01 00:00:00
abstract::Studies from Africa have demonstrated that black people have higher vitamin B12 (cobalamin) levels than do white people. The authors compared healthy white people, black people, and Latin-Americans in the United States. Their study is also the largest in which the effect of race and sex on the cobalamin levels has bee...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/88.1.95
更新日期:1987-07-01 00:00:00
abstract::Determination of HER-2/neu oncogene amplification has become clinically important in the present management of breast cancer and may have important applications in other areas of clinical oncology and scientific research. In situ hybridization is an extremely accurate and sensitive technique for assessing amplificatio...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/p40p2ead42pukdmg
更新日期:2003-03-01 00:00:00
abstract::The clinical and pathologic features of 64 inflammatory fibroid polyps of the large and small bowel in 63 patients are reported. Six of the lesions in the small bowel were studied by electron microscopy. Follow-up information was obtained from 39 patients. The lesion was always benign, occurred at all ages, and had a ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/81.6.708
更新日期:1984-06-01 00:00:00
abstract:OBJECTIVES:To explore the survival and definition of stage IA mixed endometrial carcinoma. METHODS:From June 1, 2010, to June 1, 2017, cases with stage IA endometrial cancer were included in this study. The survival outcomes were compared among patients with endometrioid (group A), nonendometrioid (group B), and mixed...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqz083
更新日期:2019-10-07 00:00:00
abstract::We sought to determine the sensitivity and specificity of immunohistochemistry using the TORDJI-22 MoAb (BioGenex, San Ramon, Calif), which is specific for the C-100 protein of the hepatitis C virus, compared with reverse transcriptase-polymerase chain reaction (RT-PCR) of tissue for viral RNA. RT-PCR had been perform...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/110.1.32
更新日期:1998-07-01 00:00:00
abstract::In this study, I have estimated the percentage of tumor by visual estimate in 447 prostatectomy specimens from American Veterans Affairs patients and related this measurement to overall survival. Although percentage of tumor was significantly related to the serum prostate-specific antigen level, tumor stage, and Gleas...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPX5MAMNMFE6FQ
更新日期:2009-01-01 00:00:00
abstract::Blood gas measurements performed at the central laboratory and at a satellite laboratory of Brigham and Women's Hospital were studied to determine the differences in quality, turnaround time (TAT), and cost. The quality in both laboratories, as determined by results on proficiency and quality control samples, satisfac...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/102.1.7
更新日期:1994-07-01 00:00:00
abstract::Mean nuclear areas (MNA) were calculated morphometrically in ten cases of well-differentiated adenocarcinoma, ten cases of atypical alveolar cuboidal cell hyperplasia (AAH), and five cases of alveolar cuboidal cell hyperplasia (AH) and were compared with each other. In cases of adenocarcinoma, the MNA were significant...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.2.146
更新日期:1986-02-01 00:00:00
abstract::Monoclonal immunoradiometric assays (IRMA) for human chorionic gonadotropin (hCG) are available for either intact hCG (IhCG) or beta subunit hCG (beta hCG). The authors evaluated the clinical applications of both methods. Serum samples (N = 180) were divided into the following five clinical groups: Group 1: elevated l...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/93.3.347
更新日期:1990-03-01 00:00:00
abstract::Cytokeratin (CK) expression was studied in 22 sinonasal inverted papillomas. Columnar (respiratory) epithelium in inverted papillomas abundantly expressed CK7, CK8, CK18, and CK19. Immunoreactivity for CK5/14 and CK17 was found in basal and parabasal/suprabasal cells. Transitional (cuboidal) and squamous epithelium in...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/TH5J-T1Q6-9QJR-8CDK
更新日期:2001-05-01 00:00:00
abstract:OBJECTIVES:To examine Hedgehog signaling in cutaneous fibrosing disorders for which effective approved therapies are lacking, expand our knowledge of pathophysiology, and explore the rationale for targeted inhibition. METHODS:Stain intensity and percentage of cells staining for Sonic hedgehog (Shh), Indian hedgehog (I...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw192
更新日期:2016-12-01 00:00:00
abstract::Anti-glomerular basement membrane (anti-GBM) glomerulonephritis is a rare disease caused by IgG autoantibodies against the glomerular basement membrane. We describe clinical and pathologic findings in a series of renal biopsy specimens from 80 patients. The patients ranged in age from 16 to 87 years. The age distribut...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/nptp-4ukv-7ju3-elmq
更新日期:2006-03-01 00:00:00
abstract::The expression of human E-cadherin in normal tissues and in benign and malignant tumors of female genital organs was examined immunohistochemically with a monoclonal antibody, HECD-1, specific for human E-cadherin. The normal tissues included the ovary, fallopian tube, uterine endometrium, uterine cervix, and vagina. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/98.1.76
更新日期:1992-07-01 00:00:00
abstract::Serum creatine kinase (CK) was measured in 515 healthy white women and 28 obligate carriers for Duchenne muscular dystrophy. There was substantial overlap between the control and carrier populations. To analyze the impact of the degree of overlap, the predictive value of a CK result was determined by (1) using sensiti...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/84.5.655
更新日期:1985-11-01 00:00:00
abstract::Clotting tests based upon the activated partial thromboplastin time are disturbed by heparin. This brief communication shows that Factor XII clotting activity in plasma samples heparinized in vitro and in vivo can be measured accurately by means of an aPTT-test using congenitally deficient substrate plasma when the pl...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.4.474
更新日期:1983-10-01 00:00:00
abstract:OBJECTIVES:To characterize error rates for genetic test orders between medical specialties and in different settings by examining detailed order information. METHODS:We performed a retrospective analysis of a detailed utilization management case database, comprising 2.5 years of data and almost 1,400 genetic test orde...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw105
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVES:To examine high expression of tumor necrosis factor ligand superfamily member 13 (TNFSF13), which is correlated with several malignancies. METHODS:TNFSF13 messenger RNA expression in tumor cells and fibroblasts in a cohort of patients with non-small cell lung cancer (NSCLC) was analyzed by quantitative real...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP4JP8BZOMHEAW
更新日期:2014-02-01 00:00:00
abstract::The radioimmunoassay of ACTH was used in a routine laboratory to localize the site of the lesion in 20 patients with Cushing's syndrome. Eight of the patients had no detectable circulating ACTH and had adrenal tumors removed, 12 had high levels and were diagnosed as having pituitary Cushing's syndrome. Very high level...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/64.5.618
更新日期:1975-11-01 00:00:00
abstract::We surveyed 271 laboratories participating in a quality assessment program to ascertain whether the use of a calibration curve for determining the international normalized ratio (INR) would improve interlaboratory accuracy and precision. Lyophilized warfarinized samples with INR values assigned through manual calibrat...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/111.2.193
更新日期:1999-02-01 00:00:00
abstract::Transbronchoscopic biopsies of lung (transbronchial) or bronchus (endobronchial) have a high diagnostic of yield when performed by a single expert bronchoscopist or a small group of expert bronchoscopists. This procedure's diagnostic yield was evaluated in a general hospital where biopsies are performed by a diverse g...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/72.6.926
更新日期:1979-12-01 00:00:00
abstract::To establish the effect of a quality control failure on the performance of the LCx-GC nucleic-acid amplification assay for Neisseria gonorrhoeae (Abbott Laboratories, Abbott Park, IL) in the field, we conducted a retrospective analysis comparing the clinical and analytic performance of the recalled lots with those not...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/1LD8-LU7Y-FFW7-5WQA
更新日期:2005-06-01 00:00:00
abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.1.108
更新日期:1985-01-01 00:00:00
abstract::The results of a direct anaerobic susceptibility testing method were compared with those of a standardized method. Seventy-three blood culture isolates and eight antimicrobial agents were used. The results of the direct and standardized testing methods were within one two-fold dilution for 95.2% of the 584 anaerobe-an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.4.558
更新日期:1980-04-01 00:00:00
abstract::A 55-year-old man presented with nerve compression and examination of tissue removed by laminectomy, and bone marrow aspiration was diagnostic of multiple myeloma. Protein studies showed a total serum protein of 5.7 g/dL, with a M-component in the fast beta region. The abnormal protein reacted only with anti-lambda an...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::The thickness of eosinophilic band in collagenous colitis (CC) was assessed by 3 methods: histologic estimates (22 observers), conventional measurements using a calibrated micrometric scale (1 observer), and semiautomatic micrometric measurements (1 observer). By the histologic estimate technique, 7.4% of the results ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/K7MH8UCK3MR7Q77L
更新日期:2008-09-01 00:00:00
abstract::The ABH blood group isoantigen status of a retrospective series of 233 invasive breast carcinomas was examined, employing monoclonal antibodies (MCAB) against A, B, and H antigens with the avidin-biotin-peroxidase complex method. In addition, the H antigen was localized with Ulex Europeus Agglutinin I (UEAI) binding. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/83.3.308
更新日期:1985-03-01 00:00:00