Influence of salivary macromolecules and fluoride on enamel lesion remineralization in vitro.

Abstract:

:The aim of this study was to investigate the influence of salivary macromolecules on enamel lesion remineralization in the presence or absence of fluoride. Paraffin-stimulated whole saliva was centrifuged, and the supernatant was dialyzed in 1,000 molecular-weight cutoff dialysis tubes, first against a phosphate buffer and then against a mineral solution containing Ca and phosphate. Artificial subsurface lesions of human enamel, created in pH 4.5 acetate buffer, were remineralized for 28 days in 4 remineralizing solutions: group C--mineral solution as a control; group S--mineral solution + dialyzed saliva; group F--mineral solution + 1 ppm F; group SF--mineral solution + dialyzed saliva + 1 ppm F. Changes in relative mineral concentration in the lesions were assessed by transverse microradiography. The results showed statistically significant mineral gains in the lesion body in groups C (DeltaZ = 3,254 +/- 1,562% x microm) and SF (DeltaZ = 2,973 +/- 1,349% x microm), but not in groups S (DeltaZ = 5,192 +/- 1,863% x microm) and F (DeltaZ = 4,310 +/- 1,138% x microm) compared with the baseline group (DeltaZ = 5,414 +/- 461% x microm). It was also found that the mineral density at the surface layer in group F (75.0 +/- 15.7%) was greater than that in the baseline group (30.1 +/- 12.3%) with statistical significance, but not in group SF (39.9 +/- 16.5%). It was concluded that the macromolecules inhibited lesion remineralization fundamentally but that these molecules, in the presence of fluoride, seemed to play an important role in the continuation of remineralization by reducing mineral gains at the surface layer.

journal_name

Caries Res

journal_title

Caries research

authors

Fujikawa H,Matsuyama K,Uchiyama A,Nakashima S,Ujiie T

doi

10.1159/000111748

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

37-45

issue

1

eissn

0008-6568

issn

1421-976X

pii

000111748

journal_volume

42

pub_type

杂志文章
  • Fluoride and calcium content of enamel organ, muscle, liver and plasma in rats.

    abstract::Nine-day-old rats were given oral fluoride doses (0.5 micrograms F/g body weight). Plasma, enamel organ, muscle and liver samples were collected from nondosed pups (baseline) and 15, 30, 60 and 120 min after the dose. Samples were assayed for fluoride and calcium concentrations. Adult rats were given 20 ppm F water fo...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261449

    authors: Bawden JW,Deaton TG,Crawford BP

    更新日期:1992-01-01 00:00:00

  • Effect of partial substitution of invert sugar for sucrose in combination with Duraphat treatment on caries development in preschool children: the Malmö Study.

    abstract::The aim was to study the effect of substitution of invert sugar for sucrose, in combination with fluoride varnish (Duraphat) treatment twice a year, on caries development in preschool children. One hundred and eighty-seven 4-years-olds were divided randomly into four sugar groups: (1) sucrose (S), (2) sucrose-Duraphat...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000261381

    authors: Frostell G,Birkhed D,Edwardsson S,Goldberg P,Petersson LG,Priwe C,Winholt AS

    更新日期:1991-01-01 00:00:00

  • Effect of salivary stimulation on erosion of human and bovine enamel subjected or not to subsequent abrasion: an in situ/ex vivo study.

    abstract::This in situ/ex vivo study evaluated whether saliva stimulated by chewing gum could prevent or reduce the wear and the percent change in microhardness (%SMH) of bovine and human enamel submitted to erosion followed by brushing abrasion immediately or after 1 h. During 2 experimental 7-day crossover phases, 9 previousl...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000092229

    authors: Rios D,Honório HM,Magalhães AC,Delbem AC,Machado MA,Silva SM,Buzalaf MA

    更新日期:2006-01-01 00:00:00

  • Salivary fluoride concentration and plaque pH after using a fluoride-containing chewing gum.

    abstract::The aim was to study the salivary fluoride (F) concentration and plaque pH recovery on the chewing and the non-chewing side of the dentition during and after chewing 1 piece of chewing gum containing 0.25 mg F as NaF. Ten subjects refrained from toothbrushing for 3 days. On the fourth day, they rinsed for 1 min with 1...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000262420

    authors: Sjögren K,Lingström P,Lundberg AB,Birkhed D

    更新日期:1997-01-01 00:00:00

  • Effects of lactate dehydrogenase on fissure caries in rats.

    abstract::The effect of supplementing the diet or drinking water with lactate dehydrogenase (LDH) on the formation of caries lesions in rats was investigated. Addition of LDH [20 and 5 mg/kg in the high-sucrose (65%) diet, 2 and 1 U/ml in the drinking water (LDH-specific activity 735 U/mg solid)] resulted in significant reducti...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261236

    authors: Higham SM,Edgar WM

    更新日期:1990-01-01 00:00:00

  • Caries prevalence after cessation of water fluoridation in La Salud, Cuba.

    abstract::In the past, caries has usually increased after cessation of water fluoridation. More recently an opposite trend could be observed: DMFT remaining stable or even decreasing further. The aim of the present study conducted in La Salud (Province of Habana) in March 1997 was to analyse the current caries trend under the s...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016565

    authors: Künzel W,Fischer T

    更新日期:2000-01-01 00:00:00

  • Effects of fluoride on in vitro enamel demineralization analyzed by ¹⁹F MAS-NMR.

    abstract::The mechanistic action of fluoride on inhibition of enamel demineralization was investigated using (19)F magic angle spinning nuclear magnetic resonance (MAS-NMR). The aim of this study was to monitor the fluoride-mineral phase formed on the enamel as a function of the concentration of fluoride ions [F(-)] in the demi...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000350171

    authors: Mohammed NR,Kent NW,Lynch RJ,Karpukhina N,Hill R,Anderson P

    更新日期:2013-01-01 00:00:00

  • Inhibitory effect of antibacterial resin composite against Streptococcus mutans.

    abstract::Dental resin composites with antibacterial activity may be useful for preventing the secondary caries frequently seen around restorations. Three types of silver-supported antibacterial materials (Novaron, Amenitop and AIS) inhibited the growth of the major oral pathogen Streptococcus mutans. Minimum inhibitory amounts...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016535

    authors: Tanagawa M,Yoshida K,Matsumoto S,Yamada T,Atsuta M

    更新日期:1999-09-01 00:00:00

  • Characterization of the arginolytic microflora provides insights into pH homeostasis in human oral biofilms.

    abstract::A selected group of oral bacteria commonly associated with dental health is capable of producing alkali via the arginine deiminase system (ADS), which has a profound impact on the pH of human oral biofilms. An increased risk for dental caries has been associated with reduced ADS activity of the bacteria in oral biofil...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000365296

    authors: Huang X,Schulte RM,Burne RA,Nascimento MM

    更新日期:2015-01-01 00:00:00

  • Infant Growth and the Occurrence of Developmental Defects of Enamel in 12-Year-Olds.

    abstract:BACKGROUND/AIMS:To investigate the association between birth weight and infant growth during the first year of life and the occurrence of developmental defects of enamel (DDE) in permanent dentition. METHODS:A random sample of 668 12-year-old students was recruited from a birth cohort. Permanent incisors and first mol...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000381425

    authors: Wong HM,Peng SM,King NM,McGrath C

    更新日期:2015-01-01 00:00:00

  • Reduction of erosion by protein-containing toothpastes.

    abstract:AIM:To assess the effect of protein-containing toothpastes on the progression of dental erosion in situ (with pellicle) and in vitro (without pellicle). METHODS:A combined split-mouth (extraoral water or toothpaste brushing) and crossover (type of toothpaste) setup was used. Two protein-containing (high/low concentrat...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000344016

    authors: Jager DH,Vissink A,Timmer CJ,Bronkhorst E,Vieira AM,Huysmans MC

    更新日期:2013-01-01 00:00:00

  • Ultrasonic determination of the effect of casein phosphopeptide-amorphous calcium phosphate paste on the demineralization of bovine dentin.

    abstract::The purpose of this study was to investigate the demineralization of dentin by measuring changes in the velocity of the sonic longitudinal waves transmitted through this substrate. One group of samples was immersed in demineralization solution for 10 min twice a day and then stored in artificial saliva. Two additional...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000099319

    authors: Yamaguchi K,Miyazaki M,Takamizawa T,Inage H,Kurokawa H

    更新日期:2007-01-01 00:00:00

  • Evaluation of Fluorescence Imaging with Reflectance Enhancement (FIRE) for Quantifying Enamel Demineralization In vitro.

    abstract:OBJECTIVES:To assess the ability of fluorescence imaging with reflectance enhancement (FIRE) to quantify early enamel demineralization in vitro. METHODS:A total of 30 bovine enamel specimens were immersed in demineralizing solution to produce artificial caries. Specimens were examined by FIRE, quantitative light-induc...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000365298

    authors: Xiao Q,Tu R,He T,Yin W,Li X,Hu D,Zhang X

    更新日期:2015-01-01 00:00:00

  • Polychlorinated biphenyls cause developmental enamel defects in children.

    abstract::The aim of this study was to evaluate the effects of long-term exposure to polychlorinated biphenyls (PCBs) on developing dental enamel. 202 8- to 14-year-old children who were pre- and post-natally exposed to PCBs in the contaminated region of Bela Krajina, Slovenia, were studied. 202 controls from Brsljin were match...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016625

    authors: Jan J,Vrbic V

    更新日期:2000-11-01 00:00:00

  • The effect of adding calcium lactate to xylitol chewing gum on remineralization of enamel lesions.

    abstract::The purpose of the study was to determine whether adding calcium lactate to chewing gum containing xylitol enhances remineralization of enamel surfaces using an early caries lesion model. Enamel slabs were cut from human extracted sound teeth and artificial subsurface lesions created within each. Half the enamel slabs...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000088905

    authors: Suda R,Suzuki T,Takiguchi R,Egawa K,Sano T,Hasegawa K

    更新日期:2006-01-01 00:00:00

  • Mutans streptococci, lactobacilli and caries experience in cystic fibrosis homozygotes, heterozygotes and healthy controls.

    abstract::Mutans streptococci (MS) and lactobacillus (LB) counts (CRT Bacteria chair-side test) were compared and correlated with the respective caries experience (DMF) of cystic fibrosis (CF) homozygotes (n = 20), CF heterozygotes (n = 20) and healthy controls (n = 20). Each group of subjects was divided into two equal age gro...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000047483

    authors: Aps JK,Van Maele GO,Claeys G,Martens LC

    更新日期:2001-11-01 00:00:00

  • Fluoride retention in proximal plaque and saliva using two NaF dentifrices containing 5,000 and 1,450 ppm F with and without water rinsing.

    abstract::A total of 26 healthy volunteers participated in this randomized 4-leg crossover study designed to measure fluoride (F) retention in interdental plaque and saliva. Two NaF dentifrices (5,000 and 1,450 ppm F) were used, with and without postbrushing water rinsing. The 4 tooth brushing methods were carried out twice a d...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000201592

    authors: Nordström A,Birkhed D

    更新日期:2009-01-01 00:00:00

  • Individual variations in protective effects of experimentally formed salivary pellicles.

    abstract::Salivary proteins protect teeth against acid-induced softening and demineralization by forming a pellicle. However, little is known about individual, gender and ethnic variations in this effect. Therefore, we aimed to determine differences in protective effects of experimentally formed pellicles from 10 healthy young ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000213887

    authors: Bruvo M,Moe D,Kirkeby S,Vorum H,Bardow A

    更新日期:2009-01-01 00:00:00

  • Effects of sucrose on the extracellular matrix of plaque-like biofilm formed in vivo, studied by proteomic analysis.

    abstract::Previous studies have shown that sucrose promotes changes in the composition of the extracellular matrix (ECM) of plaque-like biofilm (PLB), but its effect on protein expression has not been studied in vivo. Therefore, the protein compositions of ECM of PLB formed with and without sucrose exposure were analyzed by two...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章

    doi:10.1159/000159607

    authors: Paes Leme AF,Bellato CM,Bedi G,Cury AA,Koo H,Cury JA

    更新日期:2008-01-01 00:00:00

  • Association between a New Sugar Index and Caries Experience: Results of a Cross-Sectional Field Study.

    abstract:OBJECTIVES:The aim of this study was to test the relationship between a newly developed sugar index and caries experience in 4th- and 6th-graders. MATERIALS AND METHODS:A total of 1,019 fourth-graders and 925 sixth-graders from 2 regions in Northern Germany participated in the study. Caries experience was recorded by ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000486102

    authors: Pieper K,Winter J,Heinzel-Gutenbrunner M,Margraf-Stiksrud J

    更新日期:2019-01-01 00:00:00

  • Variations among dentists in radiographic detection of occlusal caries.

    abstract::The quality of radiographic diagnosis of occlusal carries was examined with special attention to the diagnosis of lesions in dentin. Eighty-four extracted teeth, most of them with occlusal caries of different severity and some apparently sound, were radiographed. For validation purposes, the lesion depths were recorde...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261640

    authors: Espelid I,Tveit AB,Fjelltveit A

    更新日期:1994-01-01 00:00:00

  • Salivary factors in vomiting bulimics with and without pathological tooth wear.

    abstract::The increased occurrence of dental erosion from self-induced vomiting in bulimia nervosa is not linearly associated with the frequency or the duration of vomiting. Possible changes in the buffering and lubricating role of saliva in bulimia nervosa and their relationship to erosion have not been previously investigated...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000262343

    authors: Milosevic A,Dawson LJ

    更新日期:1996-01-01 00:00:00

  • Relationships between a clinical-visual scoring system and two histological techniques: a laboratory study on occlusal and approximal carious lesions.

    abstract::One aim of the present laboratory study was to determine whether a visual scoring system (ERK) developed for occlusal caries could be applied to approximal lesions. A new histological technique (autofluorescence, AF) recognises dentine that is soft and would be removed with an excavator during operative treatment. A s...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000069018

    authors: Kidd EA,Banerjee A,Ferrier S,Longbottom C,Nugent Z

    更新日期:2003-03-01 00:00:00

  • Efficacy of preventive agents for dental caries. Systemic fluorides: water fluoridation.

    abstract::The effectiveness of fluoridation has been documented by observational and interventional studies for over 50 years. Data are available from 113 studies in 23 countries. The modal reduction in DMFT values for primary teeth was 40-49% and 50-59% for permanent teeth. The pattern of caries now occurring in fluoride and l...

    journal_title:Caries research

    pub_type: 杂志文章,评审

    doi:10.1159/000261594

    authors: Murray JJ

    更新日期:1993-01-01 00:00:00

  • Binding characteristics of Streptococcus mutans for calcium and casein phosphopeptide.

    abstract::Casein phosphopeptides (CPP) stabilize amorphous calcium phosphate (ACP) and may be used to localize ACP in dental plaque, maintaining a state of supersaturation with respect to tooth enamel, reducing demineralization and enhancing remineralization [Reynolds, J Dent Res 1997;76:1587-1595]. The aim of this paper is to ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016618

    authors: Rose RK

    更新日期:2000-09-01 00:00:00

  • Partial removal of carious dentine: a multicenter randomized controlled trial and 18-month follow-up results.

    abstract:AIM:The aim of this study was to evaluate the effectiveness of partial removal of carious dentine and restoration in a single session (PDR) and stepwise excavation (SW), both of which are treatments for deep carious lesions, in Public Health Services in Brazil. METHODS: INCLUSION CRITERIA:patients ≥6 years old, perma...

    journal_title:Caries research

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1159/000344013

    authors: Maltz M,Jardim JJ,Mestrinho HD,Yamaguti PM,Podestá K,Moura MS,de Paula LM

    更新日期:2013-01-01 00:00:00

  • Effectiveness of Resin-Based Sealants with and without Fluoride Placed in a High Caries Risk Population: Multicentric 2-Year Randomized Clinical Trial.

    abstract::A split-mouth randomized clinical trial was carried out to assess the effectiveness of sealants in preventing carious ICDAS (International Caries Detection and Assessment System) 3-6 lesions within a 2-year follow-up. We evaluated the effectiveness of 2 types of resin-based sealants, with and without fluoride, their r...

    journal_title:Caries research

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1159/000486426

    authors: Muller-Bolla M,Courson F,Lupi-Pégurier L,Tardieu C,Mohit S,Staccini P,Velly AM

    更新日期:2018-01-01 00:00:00

  • Acid resistance of enamel subsurface lesions remineralized by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate.

    abstract::The aim of this clinical study was to investigate the acid resistance of enamel lesions remineralized in situ by a sugar-free chewing gum containing casein phosphopeptide-amorphous calcium phosphate nanocomplexes (CPP-ACP: Recaldent). The study utilized a double-blind, randomized, crossover design with two treatments:...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000080585

    authors: Iijima Y,Cai F,Shen P,Walker G,Reynolds C,Reynolds EC

    更新日期:2004-11-01 00:00:00

  • Minimal Gap Size and Dentin Wall Lesion Development Next to Resin Composite in a Microcosm Biofilm Model.

    abstract::This in vitro study investigated the development of dentin wall lesions next to resin composite containing very small gap sizes using an in vitro biofilm model, and evaluated whether a relevant threshold for the gap size could be established. Microcosm biofilms were grown for 14 days within small interfacial gaps betw...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000478536

    authors: Maske TT,Kuper NK,Cenci MS,Huysmans MDNJM

    更新日期:2017-01-01 00:00:00

  • Susceptibility of acid-softened enamel to mechanical wear--ultrasonication versus toothbrushing abrasion.

    abstract::The study aimed to compare the amounts of softened enamel removable by ultrasonication and by toothbrushing abrasion of briefly eroded samples. Thirty bovine enamel samples were demineralized in hydrochloric acid (pH 2.1) for 60 s and were then either brushed with 350 brushing strokes in toothpaste slurry (group A) or...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000096106

    authors: Wiegand A,Wegehaupt F,Werner C,Attin T

    更新日期:2007-01-01 00:00:00