Relationships between a clinical-visual scoring system and two histological techniques: a laboratory study on occlusal and approximal carious lesions.

Abstract:

:One aim of the present laboratory study was to determine whether a visual scoring system (ERK) developed for occlusal caries could be applied to approximal lesions. A new histological technique (autofluorescence, AF) recognises dentine that is soft and would be removed with an excavator during operative treatment. A second aim was to investigate the relationship between the visual scoring system (ERK) and AF of dentine both occlusally and approximally. The sample comprised 93 extracted teeth chosen to represent the range of visual scores on approximal and occlusal surfaces. After sectioning through the investigation site, the cut faces were examined in a stereomicroscope and the depth of demineralization was scored. Autofluorescence was viewed with a confocal laser scanning microscope. Results showed reasonable correlation between the visual scores and the stereomicroscope histological evaluations for occlusal surfaces and non-cavitated approximal surfaces. However, cavitated approximal surface lesions were less advanced histologically than cavitated occlusal carious lesions. The AF technique indicated that several lesions with intact surfaces would have had soft, excavatable dentine, whereas several with microcavities would not.

journal_name

Caries Res

journal_title

Caries research

authors

Kidd EA,Banerjee A,Ferrier S,Longbottom C,Nugent Z

doi

10.1159/000069018

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

125-9

issue

2

eissn

0008-6568

issn

1421-976X

pii

69018

journal_volume

37

pub_type

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