Abstract:
:A selected group of oral bacteria commonly associated with dental health is capable of producing alkali via the arginine deiminase system (ADS), which has a profound impact on the pH of human oral biofilms. An increased risk for dental caries has been associated with reduced ADS activity of the bacteria in oral biofilms. Arginolytic bacterial strains from dental plaque samples of caries-free and caries-active adults were isolated and characterized to investigate the basis for differences in plaque ADS activity between individuals. Fifty-six ADS-positive bacterial strains were identified by 16S rRNA gene sequencing, and their ADS activity levels were compared under standard growth conditions. The spectrum of bacterial ADS activity ranged from 45.2 to 688.0 units (mg protein)(-1). Although Streptococcus sanguinis was the most prevalent species, other Streptococcus sp. were also represented. Biochemical assays carried out using 27 ADS-positive strains under conditions known to induce or repress ADS gene expression showed substantial variation in arginolytic activity in response to pH, oxygen and the availability of carbohydrate or arginine. This study reveals that the basis for the wide spectrum of arginolytic expression observed among clinical strains is, at least in part, attributable to differences in the regulation of the ADS within and between species. The results provide insights into the microbiological basis for intersubject differences in ADS activity in oral biofilms and enhance our understanding of dental caries as an ecologically driven disease in which arginine metabolism moderates plaque pH and promotes dental health.
journal_name
Caries Resjournal_title
Caries researchauthors
Huang X,Schulte RM,Burne RA,Nascimento MMdoi
10.1159/000365296subject
Has Abstractpub_date
2015-01-01 00:00:00pages
165-76issue
2eissn
0008-6568issn
1421-976Xpii
000365296journal_volume
49pub_type
杂志文章相关文献
CARIES RESEARCH文献大全abstract::An oral health care programme in secondary schools using the atraumatic restorative treatment (ART) approach for dental caries was started in 1993. Glass ionomer (restorative type II, 1) was used as the restorative and sealant material. Sealants were placed using the 'press-finger' technique. Results after 3 years rev...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000016441
更新日期:1998-01-01 00:00:00
abstract::The purpose of this study was to investigate the demineralization of dentin by measuring changes in the velocity of the sonic longitudinal waves transmitted through this substrate. One group of samples was immersed in demineralization solution for 10 min twice a day and then stored in artificial saliva. Two additional...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000099319
更新日期:2007-01-01 00:00:00
abstract::Three animal studies were performed to investigate the influence of the macromolecular structure of milk casein on caries incidence and the possible ecological changes of the oral microbiota by such casein fractions. Towards this end, rats were infected with mixed bacterial suspensions of Streptococcus sobrinus OMZ 17...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000016550
更新日期:1999-11-01 00:00:00
abstract::Mutans streptococci (MS) and lactobacillus (LB) counts (CRT Bacteria chair-side test) were compared and correlated with the respective caries experience (DMF) of cystic fibrosis (CF) homozygotes (n = 20), CF heterozygotes (n = 20) and healthy controls (n = 20). Each group of subjects was divided into two equal age gro...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000047483
更新日期:2001-11-01 00:00:00
abstract::The aim of this study was to use ultrasonication as a method to measure subsurface demineralisation of enamel. Polished human enamel samples with surface profiles within +/-0.3 microm were divided into 6 groups of 10 specimens. The groups of specimens were exposed to 0. 3% citric acid (pH 3.2) for 30 min, 1, 2, 3 or 4...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000016604
更新日期:2000-07-01 00:00:00
abstract::This study investigated the relationship of arginine deiminase (ADS) and urease activities with dental caries through a case-control study. ADS and urease activities were measured in dental smooth-surface supragingival plaque and whole saliva samples from 93 subjects, who were in three different groups: caries-free (n...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000321139
更新日期:2010-01-01 00:00:00
abstract::The microradiographic mineral content and birefringence in water and Thoulet's solution were measured at selected points in sections of caries-like lesions. Birefringence was not related to mineral content in sound superficial enamel immersed in Thoulet's solution or in the lesion body immersed in water. For the surfa...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000261508
更新日期:1993-01-01 00:00:00
abstract::The purpose of this study was to examine plaque and saliva composition after a fluoride rinse and subsequent sucrose application. Fifteen subjects accumulated plaque for 48 h, and then rinsed with a fluoride rinse based on 228 microg/g (ppm) Na2SiF6 and some received no rinse. After 60 min, upper and lower buccal mola...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000057590
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to identify risk determinants leading to early childhood caries (ECC) and visible plaque (VP) in toddlers. Data for mother-child pairs participating in the Growing Up in Singapore towards Healthy Outcomes (GUSTO) birth cohort were collected from pregnancy to toddlerhood. Oral examinations wer...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000471811
更新日期:2017-01-01 00:00:00
abstract::New technologies have provided novel insights into how dental plaque functions as a biofilm. Confocal microscopy has confirmed that plaque has an open architecture similar to other biofilms, with channels and voids. Gradients develop in areas of dense biomass over short distances in key parameters that influence micro...
journal_title:Caries research
pub_type: 杂志文章,评审
doi:10.1159/000077756
更新日期:2004-05-01 00:00:00
abstract::This study investigated the role of a matrix metalloproteinase (MMP) inhibitor (CHX 2%) in the development of secondary caries wall lesions in different interface conditions with small (run 1) and wider gaps (run 2). Dentin discs were restored and pretreated with or without CHX 2%. In run 1, interfaces were made with ...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000490195
更新日期:2019-01-01 00:00:00
abstract::In 1995, a dental survey was performed among adults aged 25-54 in the Dutch city of 's-Hertogenbosch. A similar study was performed in the same city in 1983 and the aim of the present study was to describe trends in oral health from 1983 to 1995. National data show that the percentage of edentulous persons aged 30-39 ...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000016448
更新日期:1998-01-01 00:00:00
abstract::The effectiveness of fluoridation has been documented by observational and interventional studies for over 50 years. Data are available from 113 studies in 23 countries. The modal reduction in DMFT values for primary teeth was 40-49% and 50-59% for permanent teeth. The pattern of caries now occurring in fluoride and l...
journal_title:Caries research
pub_type: 杂志文章,评审
doi:10.1159/000261594
更新日期:1993-01-01 00:00:00
abstract::Erosion of the buccal incisor enamel, most frequent in the upper jaw, has been associated with excessive oral exposure to citric acid-containing foodstuffs. The aim of the study was to determine the retention of citric acid at different sites in the mouth after a standardized exposure. Thirty-four normal subjects rins...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000262116
更新日期:1995-01-01 00:00:00
abstract::The purpose of this study was to determine the efficacy of fluoride varnish (FV) in inhibiting progression of secondary caries (SC). In a first experiment, human teeth, restored either with amalgam or resin composite, were exposed for 4 days to a microbial caries model. Half of each specimen was then painted with an a...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000057871
更新日期:2002-03-01 00:00:00
abstract::The influence of beverage composition on the measurement of erosive potential is unclear. The aim of this study was to evaluate whether beverage composition influences the measurement of erosive potential and to evaluate the influence of exposure in small and large volumes. Eleven beverages were included: water (contr...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000116118
更新日期:2008-01-01 00:00:00
abstract::The aim of the study was to determine whether rinsing with a mouthwash after brushing with a fluoridated toothpaste affected oral fluoride (F) retention and clearance compared with an oral hygiene regime without mouthwash. In this supervised, single-blind study, 3 regimes were compared: (A) brushing for 1 min with 1 g...
journal_title:Caries research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000239753
更新日期:2009-01-01 00:00:00
abstract::This paper describes the dental health status of Italian 4-year-olds in 2004/2005 and analyzes the association between caries in preschool children and some background characteristics in children and parents. Caries was recorded according to WHO criteria. 5,538 subjects were examined. Information on the children's and...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000211719
更新日期:2009-01-01 00:00:00
abstract::To exert anticaries effects, probiotics are described to inhibit growth and biofilm formation of cariogenic bacteria such as Streptococcus mutans (SM). We screened 8 probiotics and assessed how SM growth or biofilm formation inhibition affects cariogenicity of probiotic-SM mixed-species biofilms in vitro. Growth inhib...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000452960
更新日期:2017-01-01 00:00:00
abstract::The aim of this study was to evaluate the effects of long-term exposure to polychlorinated biphenyls (PCBs) on developing dental enamel. 202 8- to 14-year-old children who were pre- and post-natally exposed to PCBs in the contaminated region of Bela Krajina, Slovenia, were studied. 202 controls from Brsljin were match...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000016625
更新日期:2000-11-01 00:00:00
abstract::Surface prismatic and aprismatic human enamel and unpolished or diamond-polished specimens of bovine enamel, with test and control surfaces on each studied block, were immersed in phosphoric acid containing cola beverage (pH 2.6) and citric or malic acid containing sports drinks (pH 2.8 and 3.4, respectively) for 15-1...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000261348
更新日期:1991-01-01 00:00:00
abstract::A questionnaire was sent to 54 experts in preventive dentistry in 23 European countries in order to make an inventory of existing national dietary guidelines for the prevention of dental caries. In addition, the experts were asked to give their personal opinion on several issues concerning the relationship between die...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000074358
更新日期:2004-01-01 00:00:00
abstract::Early childhood caries (ECC) is closely related to high numbers of mutans streptococci, lactobacilli and Candida albicans. Oral colonization of these microorganisms was monitored in a prospective clinical study in order to investigate the effect of comprehensive treatment under general anesthesia and the sustainabilit...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000351673
更新日期:2014-01-01 00:00:00
abstract::Lycasin was included in diets fed to caries-active Osborne-Mendel rats for periods up to 8 weeks. The animals could tolerate up to 15-16% of Lycasin in dry-powder diets and up to 25% in pelleted diets before gastro-intestinal disturbance. Lycasin 80/55 at 25% in the diet was non-cariogenic, producing a very low level ...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000261123
更新日期:1988-01-01 00:00:00
abstract::Gaps remain in our knowledge about the levels of fluoride intake that cause dental fluorosis. The purpose of this study was to report the fluorosis prevalence by levels of estimated fluoride intake in an effort to understand the importance of different levels of daily fluoride intake. As part of the longitudinal Iowa ...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000095648
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND/AIMS:To investigate the association between birth weight and infant growth during the first year of life and the occurrence of developmental defects of enamel (DDE) in permanent dentition. METHODS:A random sample of 668 12-year-old students was recruited from a birth cohort. Permanent incisors and first mol...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000381425
更新日期:2015-01-01 00:00:00
abstract::The aim of this in vitro study was to investigate the impact of saliva on the abrasion of eroded enamel using two measuring methods. A total of 80 bovine enamel specimens from 20 bovine incisors were allocated to four experimental groups (n = 20 specimens per group). After baseline surface microhardness (SMH) measurem...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000489133
更新日期:2019-01-01 00:00:00
abstract::The purpose of the present study was to determine the antibacterial activity of oolong tea extract on oral streptococci, including Streptococcus mutans and Streptococcus sobrinus, and to identify the response to its components. Antibacterial activity was found when the extract was added to S. mutans cells in chemicall...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000073913
更新日期:2004-01-01 00:00:00
abstract::This in situ study investigated the secondary caries development in dentin in gaps next to composite and amalgam. For 21 days, 14 volunteers wore a modified occlusal splint containing human dentin samples with an average gap of 215 µm (SD=55 µm) restored with three different materials: Filtek Supreme composite, Clearf...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000438728
更新日期:2015-01-01 00:00:00
abstract::The aim of this study was to investigate the influence of salivary macromolecules on enamel lesion remineralization in the presence or absence of fluoride. Paraffin-stimulated whole saliva was centrifuged, and the supernatant was dialyzed in 1,000 molecular-weight cutoff dialysis tubes, first against a phosphate buffe...
journal_title:Caries research
pub_type: 杂志文章
doi:10.1159/000111748
更新日期:2008-01-01 00:00:00