Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model.

Abstract:

:This study investigated the role of a matrix metalloproteinase (MMP) inhibitor (CHX 2%) in the development of secondary caries wall lesions in different interface conditions with small (run 1) and wider gaps (run 2). Dentin discs were restored and pretreated with or without CHX 2%. In run 1, interfaces were made with gaps of 30, 60, or 90 µm. Interfaces with composite placed directly onto the dentin were either bonded (Adper Single Bond 2) or not bonded. In run 2, interfaces were made with gaps of 100 µm, with or without adhesive on the composite side (CLEARFIL SE Bond). Interfaces were either bonded or not bonded, as in run 1. Microcosm biofilms were grown on dentin-composite samples for 14 days. Caries lesion outcomes were analyzed by transversal wavelength-independent microradiography at 3 locations: the outer surface, and the interface wall at a distance of 200 and 500 µm from the gap entrance. Linear regression analyses showed that pretreatment with MMP inhibitor did not influence progression of the wall lesion at any location (p ≥ 0.218). Interfaces with intentional gaps showed positive and significant effect on the wall lesion progression at 200 µm from the gap entrance (p ≤ 0.005). A small trend of increase in wall lesion development was observed at the 200-µm location when bonding was present on the composite side. In conclusion, the dentin pretreatment with CHX 2% was not able to slow down the development of secondary caries wall lesions in small and wide gaps in this biofilm model.

journal_name

Caries Res

journal_title

Caries research

authors

Maske TT,Kuper NK,Cenci MS,Huysmans MDNJM

doi

10.1159/000490195

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

107-117

issue

1

eissn

0008-6568

issn

1421-976X

pii

000490195

journal_volume

53

pub_type

杂志文章
  • Survival and Associated Risk Factors of Selective Caries Removal Treatments in Primary Teeth: A Retrospective Study in a High Caries Risk Population.

    abstract:OBJECTIVE:The aim of this retrospective study was to analyze the survival probability of selective caries removal (SCR) treatments in the primary teeth of children with high caries experience and factors potentially associated with treatment failure. METHODS:The sample included SCR treatments conducted in anterior and...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000478535

    authors: Melgar XC,Opdam NJM,Britto Correa M,Franzon R,Demarco FF,Araujo FB,Casagrande L

    更新日期:2017-01-01 00:00:00

  • A clinical and microbiological study of approximal carious lesions. Part 2: efficacy of caries removal following tunnel and class II cavity preparations.

    abstract::A randomized controlled clinical study was set up to assess caries removal following tunnel preparation (test group) and class II cavity preparation (control group). Sixty approximal lesions in adult posterior teeth, visible in the outer third of dentine on bite-wing radiographs, were referred for operative treatment....

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000047424

    authors: Ratledge DK,Kidd EA,Beighton D

    更新日期:2001-01-01 00:00:00

  • Effects of sucking acidic candy on whole-mouth saliva composition.

    abstract::Limited information is available on the effects of sucking acidic candies on saliva composition and the protective role of saliva in this relation. Therefore the aim of this study was to determine salivary effects of sucking acidic candies in vivo in relation to individual variations in whole-saliva flow rate (WSFR) a...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000088181

    authors: Jensdottir T,Nauntofte B,Buchwald C,Bardow A

    更新日期:2005-11-01 00:00:00

  • Analysis of pH-driven disruption of oral microbial communities in vitro.

    abstract::Previously, a mixed culture chemostat system was used to demonstrate that the pH generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. T...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016487

    authors: Bradshaw DJ,Marsh PD

    更新日期:1998-01-01 00:00:00

  • Caries occurrence in a fluoridated and a nonfluoridated town in Finland: a retrospective study using longitudinal data from public dental records.

    abstract::The tap water of Kuopio, Finland, was fluoridated from 1959 to 1992. In the first decade of fluoridation, children in Kuopio had lower DMF values than children in Jyväskylä, a nearby low-fluoride town, but later differences between the towns have been small and inconsistent. The present study aimed to gain further ins...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000065960

    authors: Seppä L,Hausen H,Kärkkäinen S,Larmas M

    更新日期:2002-09-01 00:00:00

  • Association between a New Sugar Index and Caries Experience: Results of a Cross-Sectional Field Study.

    abstract:OBJECTIVES:The aim of this study was to test the relationship between a newly developed sugar index and caries experience in 4th- and 6th-graders. MATERIALS AND METHODS:A total of 1,019 fourth-graders and 925 sixth-graders from 2 regions in Northern Germany participated in the study. Caries experience was recorded by ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000486102

    authors: Pieper K,Winter J,Heinzel-Gutenbrunner M,Margraf-Stiksrud J

    更新日期:2019-01-01 00:00:00

  • Effect of partial substitution of invert sugar for sucrose in combination with Duraphat treatment on caries development in preschool children: the Malmö Study.

    abstract::The aim was to study the effect of substitution of invert sugar for sucrose, in combination with fluoride varnish (Duraphat) treatment twice a year, on caries development in preschool children. One hundred and eighty-seven 4-years-olds were divided randomly into four sugar groups: (1) sucrose (S), (2) sucrose-Duraphat...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000261381

    authors: Frostell G,Birkhed D,Edwardsson S,Goldberg P,Petersson LG,Priwe C,Winholt AS

    更新日期:1991-01-01 00:00:00

  • National pathfinder survey on children's oral health in Italy: pattern and severity of caries disease in 4-year-olds.

    abstract::This paper describes the dental health status of Italian 4-year-olds in 2004/2005 and analyzes the association between caries in preschool children and some background characteristics in children and parents. Caries was recorded according to WHO criteria. 5,538 subjects were examined. Information on the children's and...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000211719

    authors: Campus G,Solinas G,Strohmenger L,Cagetti MG,Senna A,Minelli L,Majori S,Montagna MT,Reali D,Castiglia P,Collaborating Study Group.

    更新日期:2009-01-01 00:00:00

  • Powdered milk micellar casein prevents oral colonization by Streptococcus sobrinus and dental caries in rats: a basis for the caries-protective effect of dairy products.

    abstract::Three animal studies were performed to investigate the influence of the macromolecular structure of milk casein on caries incidence and the possible ecological changes of the oral microbiota by such casein fractions. Towards this end, rats were infected with mixed bacterial suspensions of Streptococcus sobrinus OMZ 17...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016550

    authors: Guggenheim B,Schmid R,Aeschlimann JM,Berrocal R,Neeser JR

    更新日期:1999-11-01 00:00:00

  • Five-year incidence of coronal and root caries in 60-, 70- and 80-year-old Swedish individuals.

    abstract::The 5-year incidence of caries was studied in a random sample of 60-, 70- and 80-year-old inhabitants of Göteborg, with 69, 51 and 28 individuals in the different age groups. One aim of the study was to introduce a root caries index (DMFRS%) in which the missing root surfaces are taken into account. This study, as wel...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000262408

    authors: Fure S

    更新日期:1997-01-01 00:00:00

  • Ultrasonic determination of the effect of casein phosphopeptide-amorphous calcium phosphate paste on the demineralization of bovine dentin.

    abstract::The purpose of this study was to investigate the demineralization of dentin by measuring changes in the velocity of the sonic longitudinal waves transmitted through this substrate. One group of samples was immersed in demineralization solution for 10 min twice a day and then stored in artificial saliva. Two additional...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000099319

    authors: Yamaguchi K,Miyazaki M,Takamizawa T,Inage H,Kurokawa H

    更新日期:2007-01-01 00:00:00

  • Protective effect of TiF(4) solutions with different concentrations and pH on development of erosion-like lesions.

    abstract:AIM:To evaluate how concentration and pH of TiF(4) influence the erosion-protective effect. METHODS:Specimens were treated with a TiF(4) solution: (1) 0.5 M F, pH 1.2, (2) 0.05 M F, pH 2.1, (3) 0.5 M F, pH 2.1, or (4) 0.05 M F, pH 1.2; then, they were exposed to HCl. RESULTS:After 2 min, the proportions of the area c...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000324155

    authors: Hove LH,Holme B,Stenhagen KR,Tveit AB

    更新日期:2011-01-01 00:00:00

  • Dental caries. Principal cause of tooth extraction in a sample of US male adults.

    abstract::Comprehensive oral examinations carried out over a period of about 10 years on participants in the Veterans Administration Dental Longitudinal Study were evaluated to identify teeth extracted during this time and to ascertain the apparent reason for these extractions. The study population included 736 dentulous adult ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261178

    authors: Chauncey HH,Glass RL,Alman JE

    更新日期:1989-01-01 00:00:00

  • Atraumatic Restorative Treatment-Sealed versus Nonsealed First Permanent Molars: A 3-Year Split-Mouth Clinical Trial.

    abstract::To investigate the efficacy of atraumatic restorative treatment (ART) sealants vs. no sealant in preventing the development of dentine caries lesions in first permanent molars over a period of 3 years. A total of 187 schoolchildren (aged 6-8 years) from a low-income population presenting the 4 first permanent molars w...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000506466

    authors: Hesse D,Guglielmi CAB,Raggio DP,Bönecker MJS,Mendes FM,Bonifácio CC

    更新日期:2020-12-16 00:00:00

  • A longitudinal study comparing mutans streptococci and lactobacilli colonisation in dentate children aged 6 to 24 months.

    abstract::This longitudinal study aimed to investigate variables associated with colonisation of mutans streptococci (MS) compared with lactobacilli (LB) colonisation in a cohort of children (n = 214) from the time of first tooth eruption at approximately 6 months until 24 months of age. Repeated plaque and salivary samples wer...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000339089

    authors: Plonka KA,Pukallus ML,Barnett AG,Walsh LJ,Holcombe TF,Seow WK

    更新日期:2012-01-01 00:00:00

  • Genes Regulating Immune Response and Amelogenesis Interact in Increasing the Susceptibility to Molar-Incisor Hypomineralization.

    abstract::Ameloblasts are sensitive cells whose metabolism and function may be affected by inflammatory stimuli. The aim of this study was to evaluate the possible association between polymorphisms in immune response-related genes and molar-incisor hypomineralization (MIH), and their interaction with polymorphisms in amelogenes...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000491644

    authors: Bussaneli DG,Restrepo M,Fragelli CMB,Santos-Pinto L,Jeremias F,Cordeiro RCL,Bezamat M,Vieira AR,Scarel-Caminaga RM

    更新日期:2019-01-01 00:00:00

  • Susceptibility of acid-softened enamel to mechanical wear--ultrasonication versus toothbrushing abrasion.

    abstract::The study aimed to compare the amounts of softened enamel removable by ultrasonication and by toothbrushing abrasion of briefly eroded samples. Thirty bovine enamel samples were demineralized in hydrochloric acid (pH 2.1) for 60 s and were then either brushed with 350 brushing strokes in toothpaste slurry (group A) or...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000096106

    authors: Wiegand A,Wegehaupt F,Werner C,Attin T

    更新日期:2007-01-01 00:00:00

  • Effect of 1-Minute Fluoride Treatment on Potential Virulence and Viability of a Cariogenic Biofilm.

    abstract::Fluoride is a well-studied and widely used agent for the prevention of dental caries. Although dental caries is strongly related to cariogenic biofilms, the effect of brief fluoride treatment on the virulence properties of biofilms has not been well studied. This study evaluated the effect of a 1-min fluoride treatmen...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000434731

    authors: Pandit S,Cai JN,Jung JE,Jeon JG

    更新日期:2015-01-01 00:00:00

  • Secondary Caries Development in in situ Gaps next to Composite and Amalgam.

    abstract::This in situ study investigated the secondary caries development in dentin in gaps next to composite and amalgam. For 21 days, 14 volunteers wore a modified occlusal splint containing human dentin samples with an average gap of 215 µm (SD=55 µm) restored with three different materials: Filtek Supreme composite, Clearf...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000438728

    authors: Kuper NK,Montagner AF,van de Sande FH,Bronkhorst EM,Opdam NJ,Huysmans MC

    更新日期:2015-01-01 00:00:00

  • Trends in caries prevalence in Dutch adults between 1983 and 1995.

    abstract::In 1995, a dental survey was performed among adults aged 25-54 in the Dutch city of 's-Hertogenbosch. A similar study was performed in the same city in 1983 and the aim of the present study was to describe trends in oral health from 1983 to 1995. National data show that the percentage of edentulous persons aged 30-39 ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016448

    authors: Kalsbeek H,Truin GJ,van Rossum GM,van Rijkom HM,Poorterman JH,Verrips GH

    更新日期:1998-01-01 00:00:00

  • Variations among dentists in radiographic detection of occlusal caries.

    abstract::The quality of radiographic diagnosis of occlusal carries was examined with special attention to the diagnosis of lesions in dentin. Eighty-four extracted teeth, most of them with occlusal caries of different severity and some apparently sound, were radiographed. For validation purposes, the lesion depths were recorde...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261640

    authors: Espelid I,Tveit AB,Fjelltveit A

    更新日期:1994-01-01 00:00:00

  • Evidence of an in vitro Coupled Diffusion Mechanism of Lesion Formation within Microcosm Dental Plaque.

    abstract::The purpose of this study was to determine whether or not the dual constant-depth film fermenter (dCDFF) is able to produce caries-like enamel lesions and to ascertain further information regarding the performance of this fully functional biological caries model. Conditions were defined by the continuation (CF) or ces...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000456015

    authors: Owens GJ,Lynch RJM,Hope CK,Cooper L,Higham SM,Valappil SP

    更新日期:2017-01-01 00:00:00

  • Effect of different concentrations of fluoride in dentifrices on dentin erosion subjected or not to abrasion in situ/ex vivo.

    abstract::This in situ/ex vivo study assessed the effect of different concentrations of fluoride in dentifrices on dentin subjected to erosion or to erosion plus abrasion. Ten volunteers took part in this crossover and double-blind study performed in 3 phases (7 days). They wore acrylic palatal appliances containing 4 bovine de...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000117807

    authors: Magalhães AC,Rios D,Moino AL,Wiegand A,Attin T,Buzalaf MA

    更新日期:2008-01-01 00:00:00

  • Efficacy of preventive agents for dental caries. Systemic fluorides: water fluoridation.

    abstract::The effectiveness of fluoridation has been documented by observational and interventional studies for over 50 years. Data are available from 113 studies in 23 countries. The modal reduction in DMFT values for primary teeth was 40-49% and 50-59% for permanent teeth. The pattern of caries now occurring in fluoride and l...

    journal_title:Caries research

    pub_type: 杂志文章,评审

    doi:10.1159/000261594

    authors: Murray JJ

    更新日期:1993-01-01 00:00:00

  • Influence of occlusion on the fluoride distribution in rat molar cementum.

    abstract::This study was undertaken to examine the influence of occlusion on the fluoride distribution in cementum following an experiment in which the occlusion in rats was locally altered by extracting the upper left molar. These and control rats with normal occlusion were given water containing 0 or 100 ppm fluoride for 12 w...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261480

    authors: Kato K,Nakagaki H,Okumura H,Li J,Weatherell JA,Robinson C

    更新日期:1992-01-01 00:00:00

  • Reduction of erosion by protein-containing toothpastes.

    abstract:AIM:To assess the effect of protein-containing toothpastes on the progression of dental erosion in situ (with pellicle) and in vitro (without pellicle). METHODS:A combined split-mouth (extraoral water or toothpaste brushing) and crossover (type of toothpaste) setup was used. Two protein-containing (high/low concentrat...

    journal_title:Caries research

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000344016

    authors: Jager DH,Vissink A,Timmer CJ,Bronkhorst E,Vieira AM,Huysmans MC

    更新日期:2013-01-01 00:00:00

  • Determination of fluoride intake from urinary fluoride excretion data in children drinking fluoridated school milk.

    abstract::Twenty-four-hour urine samples were collected from each of thirteen 5- to 6-year-old children both under customary conditions of fluoride intake (i.e. usual diet including milk containing 0.5 mg fluoride and usual oral hygiene procedures with fluoride toothpaste) and during a 4-day study period in which their customar...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000047466

    authors: Ketley CE,Lennon MA

    更新日期:2001-07-01 00:00:00

  • Effects of fluoride on in vitro enamel demineralization analyzed by ¹⁹F MAS-NMR.

    abstract::The mechanistic action of fluoride on inhibition of enamel demineralization was investigated using (19)F magic angle spinning nuclear magnetic resonance (MAS-NMR). The aim of this study was to monitor the fluoride-mineral phase formed on the enamel as a function of the concentration of fluoride ions [F(-)] in the demi...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000350171

    authors: Mohammed NR,Kent NW,Lynch RJ,Karpukhina N,Hill R,Anderson P

    更新日期:2013-01-01 00:00:00

  • Caries prevalence after cessation of water fluoridation in La Salud, Cuba.

    abstract::In the past, caries has usually increased after cessation of water fluoridation. More recently an opposite trend could be observed: DMFT remaining stable or even decreasing further. The aim of the present study conducted in La Salud (Province of Habana) in March 1997 was to analyse the current caries trend under the s...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016565

    authors: Künzel W,Fischer T

    更新日期:2000-01-01 00:00:00

  • Inhibition of Streptococcus mutans Growth and Biofilm Formation by Probiotics in vitro.

    abstract::To exert anticaries effects, probiotics are described to inhibit growth and biofilm formation of cariogenic bacteria such as Streptococcus mutans (SM). We screened 8 probiotics and assessed how SM growth or biofilm formation inhibition affects cariogenicity of probiotic-SM mixed-species biofilms in vitro. Growth inhib...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000452960

    authors: Schwendicke F,Korte F,Dörfer CE,Kneist S,Fawzy El-Sayed K,Paris S

    更新日期:2017-01-01 00:00:00