Evidence of an in vitro Coupled Diffusion Mechanism of Lesion Formation within Microcosm Dental Plaque.

Abstract:

:The purpose of this study was to determine whether or not the dual constant-depth film fermenter (dCDFF) is able to produce caries-like enamel lesions and to ascertain further information regarding the performance of this fully functional biological caries model. Conditions were defined by the continuation (CF) or cessation (FF) of a saliva-type growth medium supply during 50-mM sucrose exposures (8 times daily). Hydroxyapatite (n = 3) and bovine enamel (n = 3) substrata were included within each condition and samples extracted after 2, 4, 8, and 16 days. Community profiles were generated for fastidious anaerobes, Lactobacillus spp., Streptococcus spp., mutans streptococci (MS), and Veillonella spp. using selective culture techniques and enamel demineralisation assessed by transverse microradiography. Results demonstrated that the dCDFF model is able to produce caries-like enamel lesions with a high degree of sensitivity where reduced ionic strength within the FF condition increased surface layer mineral deposition. Between conditions, biofilm communities did not differ significantly, although MS in the biofilms extracted from the FF condition rose to a higher proportion (by 1.5 log10 units), and Veillonella spp. were initially greater within the CF condition (by 2.5 log10 units), indicating an enhanced ability for the clearance of low-pKa acids following exposures to sucrose. However, both conditions retained the ability for caries-like lesion formation.

journal_name

Caries Res

journal_title

Caries research

authors

Owens GJ,Lynch RJM,Hope CK,Cooper L,Higham SM,Valappil SP

doi

10.1159/000456015

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

188-197

issue

3

eissn

0008-6568

issn

1421-976X

pii

000456015

journal_volume

51

pub_type

杂志文章
  • The isolation of bifidobacteria from occlusal carious lesions in children and adults.

    abstract::The aim of this study was to enumerate and identify bifidobacteria from occlusal carious lesions in permanent and deciduous teeth. Samples of infected dentine were obtained from 24 active occlusal lesions in deciduous teeth and from 15 occlusal lesions in permanent teeth. Plaque samples from sound occlusal surfaces of...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000222659

    authors: Mantzourani M,Gilbert SC,Sulong HN,Sheehy EC,Tank S,Fenlon M,Beighton D

    更新日期:2009-01-01 00:00:00

  • Inhibition of Streptococcus mutans Growth and Biofilm Formation by Probiotics in vitro.

    abstract::To exert anticaries effects, probiotics are described to inhibit growth and biofilm formation of cariogenic bacteria such as Streptococcus mutans (SM). We screened 8 probiotics and assessed how SM growth or biofilm formation inhibition affects cariogenicity of probiotic-SM mixed-species biofilms in vitro. Growth inhib...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000452960

    authors: Schwendicke F,Korte F,Dörfer CE,Kneist S,Fawzy El-Sayed K,Paris S

    更新日期:2017-01-01 00:00:00

  • The Association between Sense of Coherence and Dental Caries in Low Social Status Schoolchildren.

    abstract::The relationship between dental caries and sense of coherence (SOC) has not been substantiated in children and adolescents, particularly among those with a low socioeconomic status. The aim of this study was to investigate the association between SOC and dental caries in schoolchildren from a low socioeconomic backgro...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000493537

    authors: Tomazoni F,Vettore MV,Mendes FM,Ardenghi TM

    更新日期:2019-01-01 00:00:00

  • Caries prevalence after cessation of water fluoridation in La Salud, Cuba.

    abstract::In the past, caries has usually increased after cessation of water fluoridation. More recently an opposite trend could be observed: DMFT remaining stable or even decreasing further. The aim of the present study conducted in La Salud (Province of Habana) in March 1997 was to analyse the current caries trend under the s...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016565

    authors: Künzel W,Fischer T

    更新日期:2000-01-01 00:00:00

  • Salivary concentrations of urea released from a chewing gum containing urea and how these affect the urea content of gel-stabilized plaques and their pH after exposure to sucrose.

    abstract::The objectives were to: (1) determine the salivary concentrations of urea during 20 min chewing of a sugar-free gum containing 30 mg of urea; (2) measure the degree to which this urea would diffuse into a gel-stabilized plaque; (3) study the effect of the urea on the fall and subsequent rise in pH (Stephan curve) on e...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000047473

    authors: Dawes C,Dibdin GH

    更新日期:2001-09-01 00:00:00

  • In vivo cariostatic effect of black copper cement on carious dentine.

    abstract::This study compared the effect of a copper phosphate cement (BCC) and a conventional glass ionomer cement (GIC) on carious dentine that remains under restorations in vivo. Using a split-mouth design, 45 primary molar pairs with dentine caries were sampled microbiologically. Without further removal of carious dentine, ...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000070867

    authors: Foley J,Blackwell A

    更新日期:2003-07-01 00:00:00

  • Early childhood caries is associated with genetic variants in enamel formation and immune response genes.

    abstract::Early childhood caries (ECC) is a chronic, infectious disease that affects the primary dentition of young children. It is the result of an imbalance of risk factors and protective factors that influence the disease. The aim of this study was to assess genetic and environmental factors that may contribute to ECC. Two h...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000362825

    authors: Abbasoğlu Z,Tanboğa İ,Küchler EC,Deeley K,Weber M,Kaspar C,Korachi M,Vieira AR

    更新日期:2015-01-01 00:00:00

  • Individual variations in protective effects of experimentally formed salivary pellicles.

    abstract::Salivary proteins protect teeth against acid-induced softening and demineralization by forming a pellicle. However, little is known about individual, gender and ethnic variations in this effect. Therefore, we aimed to determine differences in protective effects of experimentally formed pellicles from 10 healthy young ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000213887

    authors: Bruvo M,Moe D,Kirkeby S,Vorum H,Bardow A

    更新日期:2009-01-01 00:00:00

  • Antiplaque activity of some antimicrobial agents using a simple in vitro method.

    abstract::Some antimicrobial compounds were tested for their antiplaque activity by a simple method involving measurement of the weight of Streptococcus sobrinus plaque on a glass surface. Chlorhexidine, cetylpyridinium chloride, and n-tetradecylamine reduced the weight of plaque produced by S. sobrinus in vitro; some N-palmity...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261250

    authors: Murata Y,Miyamoto E,Ueda M

    更新日期:1990-01-01 00:00:00

  • Remineralizing Potential of a Low Fluoride Toothpaste with Sodium Trimetaphosphate: An in situ Study.

    abstract:OBJECTIVE:To evaluate the effect of a low-fluoride (F) toothpaste supplemented with sodium trimetaphosphate (TMP) on enamel remineralization in situ. DESIGN:Bovine enamel blocks were selected on the basis of their surface hardness (SH) after caries-like lesions had been induced, and randomly divided into 4 treatment g...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000449358

    authors: Takeshita EM,Danelon M,Castro LP,Cunha RF,Delbem AC

    更新日期:2016-01-01 00:00:00

  • A randomised controlled trial of the efficacy of supervised toothbrushing in high-caries-risk children.

    abstract::Scottish children have one of the highest levels of caries experience in Europe. Only 33% of 5-year-old children in Dundee who developed caries in their first permanent molars by 7 brushed their teeth twice a day. High-caries-risk children should benefit if they brush more often with fluoridated toothpaste. The aim of...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000063925

    authors: Curnow MM,Pine CM,Burnside G,Nicholson JA,Chesters RK,Huntington E

    更新日期:2002-07-01 00:00:00

  • Protective Effect of Fluoride Varnish Containing Trimetaphosphate against Dentin Erosion and Erosion/Abrasion: An in vitro Study.

    abstract::This in vitro study evaluated the protective effect of fluoride varnishes containing sodium trimetaphosphate (TMP) against dentin erosion and abrasion. Specimens of coronal dentin were divided into: placebo, 2.5% NaF, 5% NaF, 2.5% NaF + 5% TMP, and 5% NaF + 5% TMP groups (n =24/group). After single application of the ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000505179

    authors: Danelon M,Pessan JP,Prado KM,Ramos JP,Emerenciano NG,Moretto MJ,Martinhon CCR,Delbem ACB

    更新日期:2020-01-01 00:00:00

  • Antimicrobial strategies in the prevention of dental caries.

    abstract::Antimicrobial agents, applied either professionally or delivered from dentifrices or mouthwashes, could reduce caries by controlling plaque formation, suppressing cariogenic species, or by inhibiting bacterial metabolism. Chlorhexidine has proven anticaries activity; other agents might also prevent caries by means of ...

    journal_title:Caries research

    pub_type: 杂志文章,评审

    doi:10.1159/000261607

    authors: Marsh PD

    更新日期:1993-01-01 00:00:00

  • Use of transverse microradiography to quantify mineral loss by erosion in bovine enamel.

    abstract::The aim of the present study was to develop transverse microradiography (TMR) in order to quantitatively assess the influence of artificial saliva and orange juice on the erosion of bovine enamel. Bovine incisors were sectioned sagittally into two equal halves. Each half was coated with acid-resistant nail varnish exc...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016471

    authors: Amaechi BT,Higham SM,Edgar WM

    更新日期:1998-01-01 00:00:00

  • A method for the quantitative site-specific study of the biochemistry within dental plaque biofilms formed in vivo.

    abstract::The study of plaque biofilms in the oral cavity is difficult as plaque removal inevitably disrupts biofilm integrity precluding kinetic studies involving the penetration of components and metabolism of substrates in situ. A method is described here in which plaque is formed in vivo under normal (or experimental) condi...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000262398

    authors: Robinson C,Kirkham J,Percival R,Shore RC,Bonass WA,Brookes SJ,Kusa L,Nakagaki H,Kato K,Nattress B

    更新日期:1997-01-01 00:00:00

  • Histological validation of electrical resistance measurements in the diagnosis of occlusal caries.

    abstract::Diagnosis of occlusal caries is difficult from visual and radiographic examination. The use of electrical resistance measurement has been described previously and reported to be a potentially sensitive alternative technique. This is possibly due to the decreased resistance of carious pits and fissures. The aim of this...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000262152

    authors: Ricketts DN,Kidd EA,Liepins PJ,Wilson RF

    更新日期:1996-01-01 00:00:00

  • Polychlorinated biphenyls cause developmental enamel defects in children.

    abstract::The aim of this study was to evaluate the effects of long-term exposure to polychlorinated biphenyls (PCBs) on developing dental enamel. 202 8- to 14-year-old children who were pre- and post-natally exposed to PCBs in the contaminated region of Bela Krajina, Slovenia, were studied. 202 controls from Brsljin were match...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016625

    authors: Jan J,Vrbic V

    更新日期:2000-11-01 00:00:00

  • Ultrasonication as a method to study enamel demineralisation during acid erosion.

    abstract::The aim of this study was to use ultrasonication as a method to measure subsurface demineralisation of enamel. Polished human enamel samples with surface profiles within +/-0.3 microm were divided into 6 groups of 10 specimens. The groups of specimens were exposed to 0. 3% citric acid (pH 3.2) for 30 min, 1, 2, 3 or 4...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000016604

    authors: Eisenburger M,Hughes J,West NX,Jandt KD,Addy M

    更新日期:2000-07-01 00:00:00

  • Chlorhexidine, a Matrix Metalloproteinase Inhibitor and the Development of Secondary Caries Wall Lesions in a Microcosm Biofilm Model.

    abstract::This study investigated the role of a matrix metalloproteinase (MMP) inhibitor (CHX 2%) in the development of secondary caries wall lesions in different interface conditions with small (run 1) and wider gaps (run 2). Dentin discs were restored and pretreated with or without CHX 2%. In run 1, interfaces were made with ...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000490195

    authors: Maske TT,Kuper NK,Cenci MS,Huysmans MDNJM

    更新日期:2019-01-01 00:00:00

  • Caries control in overdenture patients: 18-month evaluation on fluoride and chlorhexidine therapies.

    abstract::The aim of this study was to test the clinical effects of preventive regimens using fluoride and chlorhexidine on caries development in abutment teeth in patients with overdentures. 30 patients with immediate overdentures were distributed at random among three experimental groups. In these groups three different gels ...

    journal_title:Caries research

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000261298

    authors: Keltjens HM,Schaeken MJ,van der Hoeven JS,Hendriks JC

    更新日期:1990-01-01 00:00:00

  • Protective effect of TiF(4) solutions with different concentrations and pH on development of erosion-like lesions.

    abstract:AIM:To evaluate how concentration and pH of TiF(4) influence the erosion-protective effect. METHODS:Specimens were treated with a TiF(4) solution: (1) 0.5 M F, pH 1.2, (2) 0.05 M F, pH 2.1, (3) 0.5 M F, pH 2.1, or (4) 0.05 M F, pH 1.2; then, they were exposed to HCl. RESULTS:After 2 min, the proportions of the area c...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000324155

    authors: Hove LH,Holme B,Stenhagen KR,Tveit AB

    更新日期:2011-01-01 00:00:00

  • Partial removal of carious dentine: a multicenter randomized controlled trial and 18-month follow-up results.

    abstract:AIM:The aim of this study was to evaluate the effectiveness of partial removal of carious dentine and restoration in a single session (PDR) and stepwise excavation (SW), both of which are treatments for deep carious lesions, in Public Health Services in Brazil. METHODS: INCLUSION CRITERIA:patients ≥6 years old, perma...

    journal_title:Caries research

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1159/000344013

    authors: Maltz M,Jardim JJ,Mestrinho HD,Yamaguti PM,Podestá K,Moura MS,de Paula LM

    更新日期:2013-01-01 00:00:00

  • Atraumatic Restorative Treatment-Sealed versus Nonsealed First Permanent Molars: A 3-Year Split-Mouth Clinical Trial.

    abstract::To investigate the efficacy of atraumatic restorative treatment (ART) sealants vs. no sealant in preventing the development of dentine caries lesions in first permanent molars over a period of 3 years. A total of 187 schoolchildren (aged 6-8 years) from a low-income population presenting the 4 first permanent molars w...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000506466

    authors: Hesse D,Guglielmi CAB,Raggio DP,Bönecker MJS,Mendes FM,Bonifácio CC

    更新日期:2020-12-16 00:00:00

  • Oral and Systemic Effects of Xylitol Consumption.

    abstract::Recent results of randomized trials testing the efficacy of xylitol in caries prevention have been conflicting. This narrative review reveals the sources of discrepancy. The following databases were searched for the terms "xylitol" or "artificial sweeteners" restricted to the English language: PubMed, Web of Science, ...

    journal_title:Caries research

    pub_type: 杂志文章,评审

    doi:10.1159/000499194

    authors: Janket SJ,Benwait J,Isaac P,Ackerson LK,Meurman JH

    更新日期:2019-01-01 00:00:00

  • Relationships between birefringence and mineral content in artificial caries lesions of enamel.

    abstract::The microradiographic mineral content and birefringence in water and Thoulet's solution were measured at selected points in sections of caries-like lesions. Birefringence was not related to mineral content in sound superficial enamel immersed in Thoulet's solution or in the lesion body immersed in water. For the surfa...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261508

    authors: Theuns HM,Shellis RP,Groeneveld A,van Dijk JW,Poole DF

    更新日期:1993-01-01 00:00:00

  • Residual effects of fluoride on a severe cariogenic challenge in rats.

    abstract::The influence of temporary dietary restriction with and without fluoridated drinking water on the progression of caries was studied in 60 desalivated Sprague-Dawley rats. Dietary restriction alone had little effect on caries progression when the challenge was interrupted and then resumed, in contrast to the effects of...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261974

    authors: Bowen WH,Pearson SK

    更新日期:1994-01-01 00:00:00

  • Changes in Candida spp., mutans streptococci and lactobacilli following treatment of early childhood caries: a 1-year follow-up.

    abstract::Early childhood caries (ECC) is closely related to high numbers of mutans streptococci, lactobacilli and Candida albicans. Oral colonization of these microorganisms was monitored in a prospective clinical study in order to investigate the effect of comprehensive treatment under general anesthesia and the sustainabilit...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000351673

    authors: Klinke T,Urban M,Lück C,Hannig C,Kuhn M,Krämer N

    更新日期:2014-01-01 00:00:00

  • Noncariogenicity of erythritol as a substrate.

    abstract::Erythritol is a sugar alcohol produced by Aureobasidium sp. from glucose. It is 75-80% as sweet as sucrose and is also nonhygroscopic. The aim of this study was to evaluate this sugar substitute from a cariological point of view. Erythritol was neither utilized as a substrate for the lactic acid production nor for pla...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261468

    authors: Kawanabe J,Hirasawa M,Takeuchi T,Oda T,Ikeda T

    更新日期:1992-01-01 00:00:00

  • Effects of fluoride on in vitro enamel demineralization analyzed by ¹⁹F MAS-NMR.

    abstract::The mechanistic action of fluoride on inhibition of enamel demineralization was investigated using (19)F magic angle spinning nuclear magnetic resonance (MAS-NMR). The aim of this study was to monitor the fluoride-mineral phase formed on the enamel as a function of the concentration of fluoride ions [F(-)] in the demi...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000350171

    authors: Mohammed NR,Kent NW,Lynch RJ,Karpukhina N,Hill R,Anderson P

    更新日期:2013-01-01 00:00:00

  • Effects of lactate dehydrogenase on fissure caries in rats.

    abstract::The effect of supplementing the diet or drinking water with lactate dehydrogenase (LDH) on the formation of caries lesions in rats was investigated. Addition of LDH [20 and 5 mg/kg in the high-sucrose (65%) diet, 2 and 1 U/ml in the drinking water (LDH-specific activity 735 U/mg solid)] resulted in significant reducti...

    journal_title:Caries research

    pub_type: 杂志文章

    doi:10.1159/000261236

    authors: Higham SM,Edgar WM

    更新日期:1990-01-01 00:00:00