Loss of Emi1-dependent anaphase-promoting complex/cyclosome inhibition deregulates E2F target expression and elicits DNA damage-induced senescence.

abstract：:Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conserved Saccharomyces cerevisiae histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription of PHO5, and the increase in transcript level and the higher phosphatase activity plateau...

journal_title：Molecular and cellular biology

authors： Del Rosario BC,Pemberton LF

更新日期：2008-04-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00

abstract：:p36, a major in vivo substrate of protein-tyrosine kinases, is shown to be phosphorylated at serine 25, a site very close to the major site of tyrosine phosphorylation by pp60v-src, tyrosine 23 (J. R. Glenney, Jr., and B. F. Tack, Proc. Natl. Acad. Sci. USA 82:7884-7888, 1985). We present evidence suggesting that prot...

journal_title：Molecular and cellular biology

authors： Gould KL,Woodgett JR,Isacke CM,Hunter T

更新日期：1986-07-01 00:00:00

abstract：:Thyroid hormone (T3) is critical for growth, differentiation, and maintenance of metabolic homeostasis. Mice with a knock-in mutation in the thyroid hormone receptor alpha gene (TRalpha1PV) were created previously to explore the roles of mutated TRalpha1 in vivo. TRalpha1PV is a dominant negative mutant with a framesh...

journal_title：Molecular and cellular biology

authors： Ying H,Araki O,Furuya F,Kato Y,Cheng SY

更新日期：2007-03-01 00:00:00

abstract：:Muscle wasting (cachexia) is a consequence of chronic diseases, such as cancer, and is associated with degradation of muscle proteins such as MyoD. The cytokines tumor necrosis factor alpha and gamma interferon induce muscle degeneration by activating the transcription factor NF-kappaB and its target genes. Here, we s...

journal_title：Molecular and cellular biology

authors： Di Marco S,Mazroui R,Dallaire P,Chittur S,Tenenbaum SA,Radzioch D,Marette A,Gallouzi IE

更新日期：2005-08-01 00:00:00

abstract：:Hybridization-complementation studies indicated that mutations in multiple genes can render Chinese hamster cells resistant to the alkaloid translation inhibitor emetine. Two of the genes, emtA and emtB, recognized in Chinese hamster lung and ovary cell lines, respectively, are known to affect the ribosomes of the cel...

journal_title：Molecular and cellular biology

authors： Nielsen-Smith K,McGill S,Frahm M,Roufa DJ

更新日期：1983-02-01 00:00:00

abstract：:Saccharomyces cerevisiae contains eight members of a novel and fungus-specific family of bZIP proteins that is defined by four atypical residues on the DNA-binding surface. Two of these proteins, Yap1 and Yap2, are transcriptional activators involved in pleiotropic drug resistance. Although initially described as AP-1...

journal_title：Molecular and cellular biology

authors： Fernandes L,Rodrigues-Pousada C,Struhl K

更新日期：1997-12-01 00:00:00

abstract：:The Drosophila extra sex combs (esc) protein, a member of the Polycomb group (PcG), is a transcriptional repressor of homeotic genes. Genetic studies have shown that esc protein is required in early embryos at about the time that other PcG proteins become engaged in homeotic gene repression. The esc protein consists p...

journal_title：Molecular and cellular biology

authors： Ng J,Li R,Morgan K,Simon J

更新日期：1997-11-01 00:00:00

abstract：:Flap endonuclease 1 (FEN1) has been shown to remove 5' overhanging flap intermediates during base excision repair and to process the 5' ends of Okazaki fragments during lagging-strand DNA replication in vitro. To assess the in vivo role of the mammalian enzyme in repair and replication, we used a gene-targeting approa...

journal_title：Molecular and cellular biology

authors： Larsen E,Gran C,Saether BE,Seeberg E,Klungland A

更新日期：2003-08-01 00:00:00

abstract：:Cellular differentiation is governed by changes in gene expression, but at the same time, a cell's identity needs to be maintained through multiple cell divisions during maturation. In myeloid cell lines, retinoids induce gene expression and a well-characterized two-step lineage-specific differentiation. To identify m...

journal_title：Molecular and cellular biology

authors： Balint BL,Szanto A,Madi A,Bauer UM,Gabor P,Benko S,Puskás LG,Davies PJ,Nagy L

更新日期：2005-07-01 00:00:00

abstract：:In the "canonical" view of transforming growth factor beta (TGF-beta) signaling, Smad7 plays an inhibitory role. While Smad7 represses Smad3 activation by TGF-beta, it does not reverse the inhibitory effect of TGF-beta on myogenesis, suggesting a different function in myogenic cells. We previously reported a promyogen...

journal_title：Molecular and cellular biology

authors： Miyake T,Alli NS,McDermott JC

更新日期：2010-02-01 00:00:00

abstract：:In Saccharomyces cerevisiae, double-strand breaks (DSBs) activate DNA checkpoint pathways that trigger several responses including a strong G(2)/M arrest. We have previously provided evidence that the phosphatases Ptc2 and Ptc3 of the protein phosphatase 2C type are required for DNA checkpoint inactivation after a DSB...

journal_title：Molecular and cellular biology

authors： Guillemain G,Ma E,Mauger S,Miron S,Thai R,Guérois R,Ochsenbein F,Marsolier-Kergoat MC

更新日期：2007-05-01 00:00:00

abstract：:Transcriptome profiling studies have recently uncovered a large number of noncoding RNA transcripts (ncRNAs) in eukaryotic organisms, and there is growing interest in their role in the cell. For example, in haploid Saccharomyces cerevisiae cells, the expression of an overlapping antisense ncRNA, referred to here as RM...

journal_title：Molecular and cellular biology

authors： Gelfand B,Mead J,Bruning A,Apostolopoulos N,Tadigotla V,Nagaraj V,Sengupta AM,Vershon AK

更新日期：2011-04-01 00:00:00

abstract：:SMYD lysine methyltransferases target histones and nonhistone proteins for methylation and are critical regulators of muscle development and implicated in neoplastic transformation. They are characterized by a split catalytic SET domain and an intervening MYND zinc finger domain, as well as an extended C-terminal doma...

journal_title：Molecular and cellular biology

authors： Jaiswal D,Turniansky R,Moresco JJ,Ikram S,Ramaprasad G,Akinwole A,Wolf J,Yates JR 3rd,Green EM

更新日期：2020-01-03 00:00:00

abstract：:Ischemia in the heart deprives cardiomyocytes of oxygen, triggering cell death (myocardial infarction). Ischemia and its cell culture model, hypoxia, elicit a stress response program that contributes to cardiomyocyte death; however, the molecular components required to promote this process remain nebulous. Gene 33 is ...

journal_title：Molecular and cellular biology

authors： Xu D,Patten RD,Force T,Kyriakis JM

更新日期：2006-07-01 00:00:00

abstract：:Transfection of C3H/10T1/2 cells with a c-myc gene resulted in the acquisition of responsiveness to transforming growth factor type beta. Cells transfected with an activated H-ras gene or an H-ras and c-myc gene, however, exhibited a transformed morphology and spontaneous soft-agar growth, a phenotype induced reversib...

journal_title：Molecular and cellular biology

authors： Leof EB,Proper JA,Moses HL

更新日期：1987-07-01 00:00:00

abstract：:RNA polymerase II transcribes the mRNA-encoding genes and the majority of the small nuclear RNA (snRNA) genes. The formation of a minimal functional transcription initiation complex on a TATA-box-containing mRNA promoter has been well characterized and involves the ordered assembly of a number of general transcription...

journal_title：Molecular and cellular biology

authors： Kuhlman TC,Cho H,Reinberg D,Hernandez N

更新日期：1999-03-01 00:00:00

abstract：:HO endonuclease-induced double-strand breaks in Saccharomyces cerevisiae can undergo recombination by two distinct and competing pathways. In a plasmid containing a direct repeat, in which one repeat is interrupted by an HO endonuclease cut site, gap repair yields gene conversions while single-strand annealing produce...

journal_title：Molecular and cellular biology

authors： Fishman-Lobell J,Rudin N,Haber JE

更新日期：1992-03-01 00:00:00

abstract：:By using a retrovirus-derived vector system, we generated derivatives of the human colon cancer cell line HT29 that stably overexpress a full-length cDNA encoding the beta 1 isoform of rat protein kinase C (PKC). Two of these cell lines, PKC6 and PKC7, displayed an 11- to 15-fold increase in PKC activity when compared...

journal_title：Molecular and cellular biology

authors： Choi PM,Tchou-Wong KM,Weinstein IB

更新日期：1990-09-01 00:00:00

abstract：:Transcriptional coactivator with PDZ-binding motif (TAZ) physically interacts with a variety of transcription factors and modulates their activities involved in cell proliferation and mesenchymal stem cell differentiation. TAZ is highly expressed in the kidney, and a deficiency of this protein results in multiple rena...

journal_title：Molecular and cellular biology

authors： Jang EJ,Jeong H,Han KH,Kwon HM,Hong JH,Hwang ES

更新日期：2012-12-01 00:00:00

abstract：:Cell numbers are regulated by a balance among proliferation, growth arrest, and programmed cell death. A profound example of cell homeostasis, controlled throughout life, is the complex process of blood cell development, yet little is understood about the intracellular mechanisms that regulate blood cell growth arrest...

journal_title：Molecular and cellular biology

authors： Selvakumaran M,Lin HK,Sjin RT,Reed JC,Liebermann DA,Hoffman B

更新日期：1994-04-01 00:00:00

abstract：:Two cDNAs derived from Xenopus laevis calmodulin mRNA have been cloned. Both cDNAs contain the complete protein-coding region and various lengths of untranslated segments. The two cDNAs encode an identical protein but differ from each other by 5% nucleotide substitutions. The 5' and 3' untranslated regions, to the ext...

journal_title：Molecular and cellular biology

authors： Chien YH,Dawid IB

更新日期：1984-03-01 00:00:00

abstract：:The cellular response to DNA damage is an intricate mechanism that involves the interplay among several pathways. In this study, we provide evidence of the roles of the polyadenylation factor cleavage stimulation factor 50 (CstF-50) and the ubiquitin (Ub) escort factor p97 as cofactors of BRCA1/BARD1 E3 Ub ligase, fac...

journal_title：Molecular and cellular biology

authors： Fonseca D,Baquero J,Murphy MR,Aruggoda G,Varriano S,Sapienza C,Mashadova O,Rahman S,Kleiman FE

更新日期：2018-01-29 00:00:00

abstract：:The tumor suppressor protein ARF inhibits MDM2 to activate and stabilize p53. Recent studies provided evidence for p53-independent tumor suppression functions of ARF. For example, it has been shown that ARF induces proteolysis of certain E2F species, including E2F1. In addition, ARF relocalizes E2F1 from the nucleopla...

journal_title：Molecular and cellular biology

authors： Datta A,Nag A,Raychaudhuri P

更新日期：2002-12-01 00:00:00

abstract：:The activity of p21ras is required for the proliferative response to colony-stimulating factor 1 (CSF-1), and signals transduced by both the CSF-1 receptor (CSF-1R) and p21ras stimulate transcription from promoter elements containing overlapping binding sites for Fos/Jun- and Ets-related proteins. A sequence encoding ...

journal_title：Molecular and cellular biology

authors： Langer SJ,Bortner DM,Roussel MF,Sherr CJ,Ostrowski MC

更新日期：1992-12-01 00:00:00

abstract：:The LAR family protein tyrosine phosphatases (PTPs), including LAR, PTP delta, and PTP sigma, are transmembrane proteins composed of a cell adhesion molecule-like ectodomain and two cytoplasmic catalytic domains: active D1 and inactive D2. We performed a yeast two-hybrid screen with the first catalytic domain of PTP s...

journal_title：Molecular and cellular biology

authors： Wallace MJ,Fladd C,Batt J,Rotin D

更新日期：1998-05-01 00:00:00

abstract：:Pleckstrin homology (PH) domain binding to D3-phosphorylated phosphatidylinositides (PI) provides a reversible means of recruiting proteins to the plasma membrane, with the resultant change in subcellular localization playing a key role in the activation of multiple intracellular signaling pathways. Previously we foun...

journal_title：Molecular and cellular biology

authors： Shan X,Czar MJ,Bunnell SC,Liu P,Liu Y,Schwartzberg PL,Wange RL

更新日期：2000-09-01 00:00:00

abstract：:Previous analyses defined a proliferating cell nuclear antigen (PCNA) E1A-responsive element (PERE) in the PCNA promoter that is essential for transactivation by the 243-residue product of the adenovirus type 2 E1A 12S mRNA (E1A 243R). In this report, we show that the PERE activates a heterologous basal promoter and c...

journal_title：Molecular and cellular biology

authors： Morris GF,Labrie C,Mathews MB

更新日期：1994-01-01 00:00:00

abstract：:AIRE is a transcriptional activator that directs the ectopic expression of many tissue-specific genes in medullary thymic epithelial cells, which plays an important role in the negative selection of autoreactive T cells. However, its mechanism of action remains poorly understood. In this study, we found that AIRE regu...

journal_title：Molecular and cellular biology

authors： Oven I,Brdicková N,Kohoutek J,Vaupotic T,Narat M,Peterlin BM

更新日期：2007-12-01 00:00:00

abstract：:We have examined the expression of chimeric plasmids containing coding sequences for the herpes simplex virus thymidine kinase (tk) gene or the Tn5 gene for neomycin resistance (neo) linked to the late promoter of polyoma DNA. Although polyoma late genes are generally not expressed in transformed cells containing only...

journal_title：Molecular and cellular biology

authors： Kern FG,Basilico C

更新日期：1985-04-01 00:00:00