Abstract:
BACKGROUND:The response of proximal convoluted tubules (PCTs) to angiotensin II is mediated by specific type 1 receptors found on both apical and basolateral surface membrane cells. After ligand association with type 1 receptors, different signaling pathways are triggered and determine changes in fluid absorption (Jv). The presence of AT1 and actin cytoskeleton, which are directly related to Jv, can undergo changes in distribution based on the actions of AngII and losartan. METHODS:Using a microperfusion technique and immunohistochemistry analysis, we investigated the basolateral action in PCTs, of AngII and/or losartan on Jv in rabbits, with regard to AT1 and actin cytoskeleton. RESULTS:AngII increased Jv, while in contrast, losartan and combined AngII + losartan led to its decrease. AngII did not change fluorescence intensity of AT1 receptors on tubular membranes, while losartan and AngII + losartan demonstrated a slight increase after treatment. On the other hand, AngII increased the fluorescence intensity of actin cytoskeleton, while losartan induced a decrease. AngII + losartan led actin cytoskeleton having a higher fluorescence intensity than in the control group. CONCLUSIONS:In the present study, we demonstrated that treatment of the basolateral side of PCT cells with AngII and losartan could lead to changes in absorptive tubular function. Important alterations were detected in AT1 receptor fluorescence on the luminal and basolateral membranes, and changes in F-actin cytoskeleton were verified by fluorescence following these protocols.
journal_name
J Nephroljournal_title
Journal of nephrologyauthors
Bertels IM,Gontijo JA,Figueiredo JFsubject
Has Abstractpub_date
2007-01-01 00:00:00pages
29-35issue
1eissn
1121-8428issn
1724-6059journal_volume
20pub_type
杂志文章abstract::A 59 year-old woman showed rapidly progressive glomerulonephritis during immunotherapy for metastatic renal cell carcinoma. She received unilateral nephrectomy and cytotoxic T lymphocyte (CTL) therapy for the treatment of retroperitoneal lymph node metastasis of renal cell carcinoma. With CTL therapy, her retroperiton...
journal_title:Journal of nephrology
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
abstract::Membranous glomerulopathy (MGN) is a frequent cause for nephrotic syndrome in adults. In this overview, the basic pathogenic features of Heymann nephritis, a "classical" model of MGN in rats, are compared with those of human MGN. While the pathogenic antigen(s) of rat Heymann nephritis (the polyspecific receptor prote...
journal_title:Journal of nephrology
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journal_title:Journal of nephrology
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章,评审
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-016-0324-3
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journal_title:Journal of nephrology
pub_type: 杂志文章,多中心研究
doi:10.1007/s40620-015-0208-y
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pub_type: 杂志文章
doi:10.1007/s40620-020-00781-6
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:2002-11-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-014-0107-7
更新日期:2015-02-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000133
更新日期:2013-03-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-015-0212-2
更新日期:2016-02-01 00:00:00
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journal_title:Journal of nephrology
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doi:
更新日期:2009-01-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.5301/jn.5000124
更新日期:2013-01-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:10.1007/s40620-021-00965-8
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journal_title:Journal of nephrology
pub_type: 杂志文章,多中心研究,随机对照试验
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doi:
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journal_title:Journal of nephrology
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doi:
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journal_title:Journal of nephrology
pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:
更新日期:2001-11-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章,评审
doi:
更新日期:1998-03-01 00:00:00
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journal_title:Journal of nephrology
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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pub_type: 杂志文章,评审
doi:
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pub_type: 杂志文章
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