Abstract:
:Understanding and manipulating pancreatic beta-cell proliferation is a major challenge for pancreas biology and diabetes therapy. Recent studies have raised the possibility that human beta-cells can undergo dedifferentiation and give rise to highly proliferative mesenchymal cells, which retain the potential to redifferentiate into beta-cells. To directly test whether cultured beta-cells dedifferentiate, we applied genetic lineage tracing in mice. Differentiated beta-cells were heritably labeled using the Cre-lox system, and their fate in culture was followed. We provide evidence that mouse beta-cells can undergo dedifferentiation in vitro into an insulin-, pdx1-, and glut2-negative state. However, dedifferentiated beta-cells only rarely proliferate under standard culture conditions and are eventually eliminated from cultures. Thus, the predominant mesenchymal cells seen in cultures of mouse islets are not of a beta-cell origin.
journal_name
Diabetesjournal_title
Diabetesauthors
Weinberg N,Ouziel-Yahalom L,Knoller S,Efrat S,Dor Ydoi
10.2337/db06-1654subject
Has Abstractpub_date
2007-05-01 00:00:00pages
1299-304issue
5eissn
0012-1797issn
1939-327Xpii
db06-1654journal_volume
56pub_type
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