Abstract:
:The effect of nonenzymatic glycosylation on the biologic function of human antithrombin III was evaluated using a chromogenic thrombin substrate assay in the presence of catalytic amounts of heparin. Experimental conditions that increased the rate of nonenzymatic protein glycosylation were associated with decreases in the thrombin-inhibiting activity of antithrombin III. This glycosylation-induced inhibition of heparin-catalyzed antithrombin III activity was completely reversible by preassay incubation with excess sodium heparin. These observations provide a biochemical explanation for the heparin-reversible, accelerated fibrinogen disappearance rate induced by hyperglycemia in diabetic patients. Defective inhibition of the coagulation cascade induced by excessive nonenzymatic glycosylation of antithrombin III in vivo could contribute to accumulation of fibrin in various diabetic tissues.
journal_name
Diabetesjournal_title
Diabetesauthors
Brownlee M,Vlassara H,Cerami Adoi
10.2337/diab.33.6.532subject
Has Abstractpub_date
1984-06-01 00:00:00pages
532-5issue
6eissn
0012-1797issn
1939-327Xjournal_volume
33pub_type
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