Abstract:
:Non-insulin-dependent diabetes mellitus (NIDDM) is a heterogenous disorder characterized by defects in insulin action and secretion. This study was aimed at developing a rat model in which these pathogenic factors might be studied. Male Wistar rats were injected at 2 days of age with 45 or 30 mg/kg streptozocin (STZ) or vehicle (control). Fasting plasma glucose and insulin levels were not significantly different between the two groups between 5 and 8 wk of age. At 8 wk, half of each group was randomly assigned to isocaloric diets high in either fat (59% of calories) or starch (70% of calories). After 1 wk on the diets, 45-mg/kg-STZ-administered fat-fed animals displayed significant fasting hyperglycemia (8.6 +/- 0.2 mM; P less than 0.01), which was exacerbated by the stress of anesthesia and/or cannulation, whereas no changes were observed in any of the other groups before (STZ starch fed, 6.7 +/- 0.1 mM; control fat fed, 6.8 +/- 0.1 mM; control starch fed; 6.4 +/- 0.1 mM) or after anesthesia and/or cannulation. In the 30-mg/kg-STZ animals, fat feeding did not significantly elevate plasma glucose concentration, but a significant hyperglycemic response was seen with anesthesia and/or cannulation. In all STZ groups, substantial impairment of glucose-induced insulin secretion was observed, particularly early-phase insulin secretion. Further studies indicated that STZ animals on a diet conferring normal insulin sensitivity (starch) maintained basal normoglycemia and mildly impaired (i.v.) glucose tolerance despite this gross insulin secretory defect.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Diabetesjournal_title
Diabetesauthors
Pascoe WS,Storlien LHdoi
10.2337/diab.39.2.226subject
Has Abstractpub_date
1990-02-01 00:00:00pages
226-33issue
2eissn
0012-1797issn
1939-327Xjournal_volume
39pub_type
杂志文章相关文献
DIABETES文献大全abstract:OBJECTIVE:White adipose tissue (WAT) and brown adipose tissue (BAT) play distinct roles in adaptation to changes in nutrient availability, with WAT serving as an energy store and BAT regulating thermogenesis. We previously showed that mice maintained on a leucine-deficient diet unexpectedly experienced a dramatic reduc...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0929
更新日期:2010-01-01 00:00:00
abstract::Platelet survival by 51chromium labeling was determined in six normal control subjects and in twelve patients with diabetes mellitus, before and after improved glucose control (mean HbA1c 10.5 +/- 2.2% and 7.1 +/- 1.8%, respectively). Mean platelet survival was 9.47 +/- 0.85 days for the normal subjects and 9.04 +/- 1...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.30.6.486
更新日期:1981-06-01 00:00:00
abstract::The preferential channeling of different fuels to fat and changes in the transcription profile of adipose tissue and skeletal muscle are poorly understood processes involved in the pathogenesis of obesity and insulin resistance. Carbohydrate and lipid metabolism may play relevant roles in this context. Freely moving l...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.3.601
更新日期:2001-03-01 00:00:00
abstract::Protein kinase C (PKC) has been suggested as a mediator of insulin's effect on glucose transport, and PKC-mediated modulation of tyrosine kinase activity in the insulin receptor has been implicated in regulating the insulin sensitivity of tissues. Because skeletal muscle is a major target of insulin action, we examine...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.5.499
更新日期:1988-05-01 00:00:00
abstract::Duct-occluded segmental-pancreas transplants develop progressive fibrotic atrophy, even though endocrine function seems to be unaffected. To determine end-stage size of the graft and to evaluate magnetic resonance imaging (MRI) and computed tomography (CT), these imaging techniques were applied in eight patients with ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.1.s24
更新日期:1989-01-01 00:00:00
abstract::Beta,beta'-methyl-substituted hexadecanedioic acid (MEDICA 16) consists of a nonmetabolizable long-chain fatty acid designed to probe the effect exerted by fatty acids on insulin sensitivity. The effect of MEDICA 16 was evaluated in insulin-resistant Zucker (fa/fa) rats in terms of liver, muscle, and adipose tissue re...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.46.12.1958
更新日期:1997-12-01 00:00:00
abstract::A new murine model of porcine islet-like cell cluster (ICC) xenograft rejection, avoiding interference of unspecific inflammation, was introduced and used to investigate rejection mechanisms. Athymic (nu/nu) mice were transplanted with syngeneic, allogeneic, or xenogeneic islets under the kidney capsule. After the ori...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.5.1111
更新日期:2003-05-01 00:00:00
abstract::Glucose controls islet beta-cell mass and function at least in part through the phosphatidylinositol 3-kinase (PI3K)/Akt pathway downstream of insulin signaling. The Foxo proteins, transcription factors known in other tissues to be negatively regulated by Akt activation, affect proliferation and metabolism. In this st...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db05-0678
更新日期:2006-06-01 00:00:00
abstract::Human fetal pancreas has been obtained after the therapeutic termination of pregnancy with prostaglandin F2 alpha. Pancreatic explants were studied in a perifusion system and maintained in organ culture for up to 3 wk. Insulin biosynthesis was stimulated by glucose; however, the incorporation of 3H-L-leucine into proi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.29.1.s57
更新日期:1980-01-01 00:00:00
abstract::Studies were conducted to explore altered substrate utilization and metabolism in GLUT4 null mice. Liver fatty acid synthase mRNA and fatty acid synthesis rates were dramatically increased in GLUT4 null mice compared with control mice and were supported by increased rates of the pentose phosphate pathway oxidative pha...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.4.935
更新日期:2005-04-01 00:00:00
abstract::Linkage results suggest that the region of chromosome 7 containing the leptin gene cosegregates with extreme obesity; however, leptin coding region mutations are rare. To investigate whether the leptin flanking sequence and/or a larger 40-cM region (7q22.1-7q35) contributes to obesity, we genotyped individuals from 20...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.6.1557
更新日期:2003-06-01 00:00:00
abstract::This article examines the role of the autonomic nervous system in mediating the increase of glucagon secretion observed during insulin-induced hypoglycemia (IIH). In the first section, we briefly review the importance of the alpha-cell response in recovery from hypoglycemia under both physiologic conditions and pathop...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.47.7.995
更新日期:1998-07-01 00:00:00
abstract::Myoblasts from human skeletal muscle were isolated from needle biopsy samples of vastus lateralis and fused to differentiated multinucleated myotubes. Specific high-affinity insulin and insulin-like growth factor I (IGF-I) binding, glucose transporter proteins GLUT1 and GLUT4, glycogen synthase and pyruvate dehydrogen...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.44.8.936
更新日期:1995-08-01 00:00:00
abstract::Postmortem studies have shown a relationship between diabetic state and atherosclerotic arterial lesions in adolescents. The aim of the present study was to determine the presence of increased subclinical atherosclerosis (measured as carotid intima-media thickness [IMT]) and its risk factors, including lipoprotein oxi...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.2.493
更新日期:2002-02-01 00:00:00
abstract:OBJECTIVE:Obesity causes insulin resistance, which has been interpreted as reduced downstream insulin signaling. However, changes in access of insulin to sensitive tissues such as skeletal muscle may also play a role. Insulin injected directly into skeletal muscle diffuses rapidly through the interstitial space to caus...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0839
更新日期:2010-03-01 00:00:00
abstract::It has been proposed that insulin-mediated changes in muscle perfusion modulate insulin-mediated glucose uptake. However, the putative effects of insulin on the microcirculation that permit such modulation have not been studied in humans. We examined the effects of systemic hyperinsulinemia on skin microvascular funct...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/diabetes.51.5.1515
更新日期:2002-05-01 00:00:00
abstract::Decreased retinal blood flow has been measured in streptozotocin (STZ)-induced diabetes of 1 week's duration, and primary insulin intervention was effective in maintaining normal retinal blood flow in diabetic rats. Retinal blood-flow abnormalities precede clinical diabetic retinopathy in both diabetic animals and pat...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.47.4.653
更新日期:1998-04-01 00:00:00
abstract::The adipocyte hormone adiponectin is negatively correlated with obesity and insulin resistance and may exert an important antidiabetes function. In this study, primary human skeletal muscle cells were cocultured with human fat cells or incubated with adipocyte-conditioned medium in the presence or absence of the globu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.7.2003
更新日期:2005-07-01 00:00:00
abstract::There is controversy over the extent to which fatty acids (FAs) derived from plasma free FAs (FFAs) or from hydrolysis of plasma triglycerides (TGFAs) form communal or separate pools and what the contribution of each FA source is to cellular FA metabolism. Chylomicrons and lipid emulsions were labeled with [(3)H]triol...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.3.614
更新日期:2003-03-01 00:00:00
abstract::The interaction between insulin and its receptor was investigated using both monoclonal and polyclonal anti-insulin antibodies. After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.8.778
更新日期:1984-08-01 00:00:00
abstract::In type 1 diabetes, a lifelong autoimmune disease, T cells infiltrate the islets and the exocrine pancreas in high numbers. CD8+ T cells are the main cell type found in the insulitic lesion, and CD8+ T cells reactive against beta cell antigens have been detected in the periphery and in the pancreas of subjects with sh...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0908
更新日期:2021-01-07 00:00:00
abstract::Sera from rats inoculated with in vitro glucosylated rat skin acid-soluble collagen were evaluated for specific antibody response using the enzyme-linked immunosorbent assay. The data indicate that the altered protein is capable of eliciting the production of antibodies, but unmodified collagen is not. The specificity...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1182
更新日期:1983-12-01 00:00:00
abstract::The present study was performed to assess the potential relationship between the low T3 syndrome and hypothyroidism. Comparative studies were performed on the relative effects of diabetes and insulin on heparin-releasable adipose lipoprotein lipase (LPL) in the intact and hypothyroid rat. Hypothyroidism for 10 days (T...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.34.12.1266
更新日期:1985-12-01 00:00:00
abstract:OBJECTIVE:Given the pleiotropic effect of eicosapentaenoic acid (EPA), it is interesting to know whether EPA is capable of improving obesity. Here we examined the anti-obesity effect of EPA in mice with two distinct models of obesity. RESEARCH DESIGN AND METHODS:Male C57BL/6J mice were fed a high-fat/high-sucrose diet...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-1554
更新日期:2010-10-01 00:00:00
abstract::Orexins (hypocretins) are novel neuropeptides that appear to play a role in the regulation of energy balances. Orexin-A (OXA) increases food intake in rodents, and fasting activates OXA neurons in both the lateral hypothalamic area and gut. OXA is also found in the endocrine pancreas; however, little is known about it...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.1.111
更新日期:2003-01-01 00:00:00
abstract::Alzheimer disease and type 2 diabetes are characterized by increased prevalence with aging, a genetic predisposition, and comparable pathological features in the islet and brain (amyloid derived from amyloid beta protein in the brain in Alzheimer disease and islet amyloid derived from islet amyloid polypeptide in the ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.2.474
更新日期:2004-02-01 00:00:00
abstract::Unacylated ghrelin (UAG) is the predominant ghrelin isoform in the circulation. Despite its inability to activate the classical ghrelin receptor, preclinical studies suggest that UAG may promote β-cell function. We hypothesized that UAG would oppose the effects of acylated ghrelin (AG) on insulin secretion and glucose...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/db13-1598
更新日期:2014-07-01 00:00:00
abstract::The aim of this randomized double-blind study was to compare the within-subject variability of the glucose-lowering effect of a novel insulin analog, insulin detemir, with that of insulin glargine and NPH insulin in people with type 1 diabetes. Fifty-four subjects (32 males and 22 females, age 38 +/- 10 years [mean +/...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diabetes.53.6.1614
更新日期:2004-06-01 00:00:00
abstract::Obesity and body fat distribution are important risk factors for the development of type 2 diabetes and metabolic syndrome. Evidence has accumulated that this risk is related to intrinsic differences in behavior of adipocytes in different fat depots. In the current study, we demonstrate that adipocyte precursor cells ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-1753
更新日期:2012-07-01 00:00:00
abstract::Local glucocorticoid (GC) action depends on intracellular GC metabolism by 11beta-hydroxysteroid dehydrogenases (11betaHSDs). 11betaHSD1 activates GCs, while 11betaHSD2 inactivates GCs. Adipocyte-specific amplification of GCs through transgenic overexpression of 11betaHSD1 produces visceral obesity and the metabolic s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.4.1023
更新日期:2005-04-01 00:00:00