当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Phosphoprotein and phosphopeptide interactions with the FHA domain from Arabidopsis kinase-associated protein phosphatase.

Phosphoprotein and phosphopeptide interactions with the FHA domain from Arabidopsis kinase-associated protein phosphatase.

Abstract：

:FHA domains are phosphoThr recognition modules found in diverse signaling proteins, including kinase-associated protein phosphatase (KAPP) from Arabidopsis thaliana. The kinase-interacting FHA domain (KI-FHA) of KAPP targets it to function as a negative regulator of some receptor-like kinase (RLK) signaling pathways important in plant development and environmental responses. To aid in the identification of potential binding sites for the KI-FHA domain, we predicted (i) the structure of a representative KAPP-binding RLK, CLAVATA1, and (ii) the functional surfaces of RLK kinase domains using evolutionary trace analysis. We selected phosphopeptides from KAPP-binding Arabidopsis RLKs for in vitro studies of association with KI-FHA from KAPP. Three phosphoThr peptide fragments from the kinase domain of CLV1 or BAK1 were found to bind KI-FHA with KD values of 8-20 microM, by NMR or titration calorimetry. Their affinity is driven by favorable enthalpy and solvation entropy gain. Mutagenesis of these three threonine sites suggests Thr546 in the C-lobe of the BAK1 kinase domain to be a principal but not sole site of KI-FHA binding in vitro. The brassinosteroid receptor BRI1 and KAPP are shown to associate in vivo and in vitro. Further genetic studies indicate that KAPP may be a negative regulator of the BRI1 signaling transduction pathway. 15N-Labeled KI-FHA was titrated with the GST-BRI1 kinase domain and monitored by NMR. BRI1 interacts with the same 3/4, 4/5, 6/7, 8/9, and 10/11 recognition loops of KI-FHA, with similar affinity as the phosphoThr peptides.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ding Z,Wang H,Liang X,Morris ER,Gallazzi F,Pandit S,Skolnick J,Walker JC,Van Doren SR

doi

10.1021/bi061763n

subject

Has Abstract

pub_date

2007-03-13 00:00:00

pages

2684-96

issue

10

eissn

0006-2960

issn

1520-4995

journal_volume

46

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Role of a heterogeneous free state in the formation of a specific RNA-theophylline complex.

    abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi027103+

    authors： Jucker FM,Phillips RM,McCallum SA,Pardi A

    更新日期：2003-03-11 00:00:00

  • Purification of histamine receptor proteins from detergent-solubilized human peripheral blood mononuclear cells.

    abstract：:Histamine is released from mast cells and basophils by either immunological or nonimmunological mechanisms. Histamine, which is the most potent short acting mediator released from these cells, exerts its diverse biological actions by binding to cell surface histamine receptors. We report the affinity purification of h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00182a008

    authors： Warlow RS,Rajasekariah P,Lambert P,Morgan J,Dao LP,Bernard CC,Walls RS

    更新日期：1994-04-26 00:00:00

  • Effects of sterol structure and exogenous lipids on the transbilayer distribution of sterols in the membrane of Mycoplasma capricolum.

    abstract：:Stopped-flow kinetic measurements of the association of filipin with sterols in intact cells and isolated membranes of Mycoplasma capricolum were used to study the effects of varying the phospholipid in the membrane. The phospholipid composition and content of the membrane were varied by growing cells in an albumin-co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00511a019

    authors： Clejan S,Bittman R,Rottem S

    更新日期：1981-04-14 00:00:00

  • Conformational Closure of the Catalytic Site of Human CD38 Induced by Calcium.

    abstract：:First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：

    authors： Liu Q,Graeff R,Kriksunov IA,Lam CM,Lee HC,Hao Q

    更新日期：2008-12-30 00:00:00

  • A large solvent isotope effect on protein association thermodynamics.

    abstract：:Solvent reorganization can contribute significantly to the energetics of protein-protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a prerequisite to DNA bindin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400952m

    authors： Eginton C,Beckett D

    更新日期：2013-09-24 00:00:00

  • Expression and sequence analyses of serum amyloid A in the Syrian hamster.

    abstract：:Reactive amyloidosis occurs during chronic inflammation and involves deposition of amyloid A (AA) fibrils in many organs. Amyloid A is derived by proteolysis from serum amyloid A component (SAA), a major acute-phase reactant in many species. Since spontaneous amyloidosis occurs commonly in Syrian hamsters, we have stu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00437a040

    authors： Webb CF,Tucker PW,Dowton SB

    更新日期：1989-05-30 00:00:00

  • Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis.

    abstract：:Three mutants, Asp55-Gly, Tyr306-->Phe, and Asp309-->Asn, of Aspergillus awamori glucoamylase (identical to Aspergillus niger glucoamylase) were constructed to elucidate the roles of two conserved regions within fungal glucoamylases. Kinetic studies indicate that both of these regions are closely associated with activ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00055a017

    authors： Sierks MR,Svensson B

    更新日期：1993-02-02 00:00:00

  • Carbohydrate chains of human thyrotropin are differentially susceptible to endoglycosidase removal on combined and free polypeptide subunits.

    abstract：:The accessibility of the asparagine-linked carbohydrate chains of human thyrotropin (hTSH) and free alpha and beta subunits was investigated by their susceptibility to endoglycosidases H and F as well as to peptide:N-glycosidase F. Iodinated hTSH or subunits were incubated with a commercial enzyme preparation containi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00392a040

    authors： Ronin C,Papandreou MJ,Canonne C,Weintraub BD

    更新日期：1987-09-08 00:00:00

  • Monoclonal antibodies to poly(adenosine diphosphate ribose) recognize different structures.

    abstract：:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a032

    authors： Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura T

    更新日期：1984-07-31 00:00:00

  • Spectroscopic and magnetic studies of human ceruloplasmin: identification of a redox-inactive reduced Type 1 copper site.

    abstract：:Ceruloplasmin is unique among the multicopper oxidases in that in addition to the usual copper stoichiometry of one Type 1 copper site and a Type 2/Type 3 trinuclear copper cluster, it contains two other Type 1 sites. This assignment of copper sites, based on copper quantitation, sequence alignment, and crystallograph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980434v

    authors： Machonkin TE,Zhang HH,Hedman B,Hodgson KO,Solomon EI

    更新日期：1998-06-30 00:00:00

  • Direct measurement of the weak interactions between a mouse Fc receptor (Fc gamma RII) and IgG1 in the absence and presence of hapten: a total internal reflection fluorescence microscopy study.

    abstract：:Total internal reflection fluorescence microscopy (TIRFM) has been used to directly measure the weak dissociation constants of IgG with a mouse IgG receptor (moFc gamma RII) that has been purified and reconstituted into substrate-supported planar membranes. Dissociation constants were measured for three different mous...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00161a039

    authors： Hsieh HV,Poglitsch CL,Thompson NL

    更新日期：1992-11-24 00:00:00

  • Self base pairing in a complementary deoxydinucleoside monophosphate duplex: crystal and molecular structure of deoxycytidylyl-(3'-5')-deoxyguanosine.

    abstract：:The molecular structure of ammonium deoxycytidylyl-(3'-5')-deoxyguanosine, crystallized from aqueous acetone near pH 4, was determined for X-ray diffraction data. The crystals were tetragonal, space group P43212 with a = b = 11.078 (1) A and c = 45.826 (4) A. The structure was solved by tangent expansion of phases bas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a014

    authors： Cruse WB,Egert E,Kennard O,Sala GB,Salisbury SA,Viswamitra MA

    更新日期：1983-04-12 00:00:00

  • Mutational analysis of the "regulatory module" of hormone-sensitive lipase.

    abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049206t

    authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

    更新日期：2005-02-15 00:00:00

  • Effects of DNA binding proteins on DNA methylation in vitro.

    abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00326a021

    authors： Kautiainen TL,Jones PA

    更新日期：1985-02-26 00:00:00

  • Three-dimensional structure of p-cresol methylhydroxylase (flavocytochrome c) from Pseudomonas putida at 3.0-A resolution.

    abstract：:p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00215a034

    authors： Mathews FS,Chen ZW,Bellamy HD,McIntire WS

    更新日期：1991-01-08 00:00:00

  • The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and depolymerization.

    abstract：:Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament severing and pointed-end depolymerization. Multicellular organisms express multiple ADF/cofilin isoforms in a tissue-specific manner, and the vertebrate proteins are grouped into ADFs and cofilins o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050933d

    authors： Yamashiro S,Mohri K,Ono S

    更新日期：2005-11-01 00:00:00

  • Effect of methionine oxidation on the structural properties, conformational stability, and aggregation of immunoglobulin light chain LEN.

    abstract：:Light chain amyloidoses arise from the overproduction and abnormal deposition of the immunoglobulin light chain in various organs. LEN is the variable domain of an immunoglobulin light chain originally isolated from the urine of a patient suffering from multiple myeloma, with no sign of renal dysfunction or amyloidosi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800806d

    authors： Hu D,Qin Z,Xue B,Fink AL,Uversky VN

    更新日期：2008-08-19 00:00:00

  • Human pulmonary surfactant protein D binds the extracellular domains of Toll-like receptors 2 and 4 through the carbohydrate recognition domain by a mechanism different from its binding to phosphatidylinositol and lipopolysaccharide.

    abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060176z

    authors： Ohya M,Nishitani C,Sano H,Yamada C,Mitsuzawa H,Shimizu T,Saito T,Smith K,Crouch E,Kuroki Y

    更新日期：2006-07-18 00:00:00

  • Structural studies on the active site of Escherichia coli RNA polymerase. 2. Geometrical relationship of the interacting substrates.

    abstract：:Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00477a017

    authors： Beal RB,Pillai RP,Chuknyisky PP,Levy A,Tarien E,Eichhorn GL

    更新日期：1990-06-26 00:00:00

  • Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase.

    abstract：:Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048201t

    authors： Qian M,Ajandouz el H,Payan F,Nahoum V

    更新日期：2005-03-08 00:00:00

  • Formation and rejoining of deoxyribonucleic acid double-strand breaks induced in isolated cell nuclei by antineoplastic intercalating agents.

    abstract：:The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a013

    authors： Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

    更新日期：1984-07-03 00:00:00

  • Abasic frameshift in DNA. Solution conformation determined by proton NMR and molecular mechanics calculations.

    abstract：:We have determined the three-dimensional structure of a non-self-complementary oligodeoxynucleotide duplex that contains a model abasic site. The duplex contains six GC base pairs plus the abasic site at the center of one strand and corresponds to an abasic frameshift. Two-dimensional NMR studies on the nonexchangeabl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00431a009

    authors： Cuniasse P,Sowers LC,Eritja R,Kaplan B,Goodman MF,Cognet JA,Le Bret M,Guschlbauer W,Fazakerley GV

    更新日期：1989-03-07 00:00:00

  • Induction of stable protein-deoxyribonucleic acid adducts in Chinese hamster cell chromatin by ultraviolet light.

    abstract：:Ultraviolet (uv)-light-mediated formation of protein-DNA adducts in Chinese hamster cell chromatin was investigated in an attempt to compare chromatin alterations induced in vitro with those observed in vivo. Three independent methods of analysis indicated stable protein-DNA associations: (1) a membrane filter assay w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00653a019

    authors： Strniste GF,Rall SC

    更新日期：1976-04-20 00:00:00

  • Binding of calcium to anticoagulant protein S: role of the fourth EGF module.

    abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601151

    authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

    更新日期：2006-09-05 00:00:00

  • Refined 1.8-A structure reveals the mode of binding of beta-cyclodextrin to the maltodextrin binding protein.

    abstract：:The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00091a004

    authors： Sharff AJ,Rodseth LE,Quiocho FA

    更新日期：1993-10-12 00:00:00

  • Nucleotide- and substrate-induced conformational transitions in the CBS domain-containing pyrophosphatase of Moorella thermoacetica.

    abstract：:In contrast to all other known pyrophosphatases, Moorella thermoacetica pyrophosphatase (mtCBS-PPase) contains nucleotide-binding CBS domains and is thus strongly regulated by adenine nucleotides. Stopped-flow measurements using a fluorescent AMP analogue, 2'(3')-O-(N-methylanthranoyl)-AMP (Mant-AMP), reveal that nucl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9019737

    authors： Jämsen J,Baykov AA,Lahti R

    更新日期：2010-02-09 00:00:00

  • solution structure, backbone dynamics, and interaction with Cdc42 of Salmonella guanine nucleotide exchange factor SopE2.

    abstract：:SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0490744

    authors： Williams C,Galyov EE,Bagby S

    更新日期：2004-09-28 00:00:00

  • Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.

    abstract：:Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00504a600

    authors： Johnson FA,Lewis SD,Shafer JA

    更新日期：1981-01-06 00:00:00

  • Groove- and sequence-selective alkylation of DNA by sulfonate esters tethered to lexitropsins.

    abstract：:A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00082a017

    authors： Zhang Y,Chen FX,Mehta P,Gold B

    更新日期：1993-08-10 00:00:00

  • Regulation of plasminogen activation by components of the extracellular matrix.

    abstract：:The kinetics of activation of Glu-plasminogen (Glu-Pg) and Lys77-Pg by two-chain recombinant tissue plasminogen activator (t-PA) were determined in the presence of isolated protein components of the extracellular matrix (ECM) and compared to activation in the presence of fibrinogen and fibrinogen fragments and in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00472a029

    authors： Stack S,Gonzalez-Gronow M,Pizzo SV

    更新日期：1990-05-22 00:00:00