Immunization of eyed channel catfish, Ictalurus punctatus, eggs with monovalent Flavobacterium columnare vaccine and bivalent F. columnare and Edwardsiella ictaluri vaccine.


:The efficacy of a modified live monovalent Flavobacterium columnare vaccine and bivalent F. columnare and Edwardsiella ictaluri vaccines were evaluated following immersion vaccination of eyed channel catfish (Ictalurus punctatus) eggs. The modified live F. columnare vaccine was grown in modified Shieh broth and administered at 1.35 x 10(7) CFU/ml for 15 min exposure (1l water). Booster immunization was conducted at day 34 with 2.17 x 10(7) CFU/ml for 15 min. Bivalent vaccines consisted of a 1:1 ratio of the modified live F. columnare and AQUAVAC-ESC vaccine for the 15 min exposure (1l immersion bath). Non-vaccinated controls were held in 1l water without vaccine for 15 min. Fish were challenged with F. columnare (ALG-00-530) by immersion at days 109, 116, and 137 post-primary immunization or E. ictaluri (AL-93-75) by immersion at day 116 (bivalent vaccine group). Efficacy of monovalent modified live F. columnare vaccine administered singly or with a booster vaccination was shown to be protective with relative percent survival (RPS) values ranging from 50.0 to 76.8. Some variation was seen in RPS values following bivalent immunization, ranging from 33.0 to 59.7 in the fish challenged with F. columnare and 44.5 to 66.7 in fish challenged with E. ictaluri. However, the RPS values were not statistically different. The results suggest that administration of live bivalent vaccine at the eyed-egg stage is safe and elicits protection upon single pathogen challenge.






Shoemaker CA,Klesius PH,Evans JJ




Has Abstract


2007-01-22 00:00:00
















  • Comparative evaluation of oral and intranasal priming with replication-competent adenovirus 5 host range mutant (Ad5hr)-simian immunodeficiency virus (SIV) recombinant vaccines on immunogenicity and protective efficacy against SIV(mac251).

    abstract::Oral, replication-competent Ad-HIV vaccines are advancing to human trials. Previous evaluation of protective efficacy in non-human primates has primarily followed upper respiratory tract administrations. Here we compared sequential oral (O/O) versus intranasal/oral (I/O) priming of rhesus macaques with Ad5 host range ...


    pub_type: 杂志文章


    authors: Zhou Q,Hidajat R,Peng B,Venzon D,Aldrich MK,Richardson E,Lee EM,Kalyanaraman VS,Grimes G,Gómez-Román VR,Summers LE,Malkevich N,Robert-Guroff M

    更新日期:2007-11-19 00:00:00

  • Mutant influenza A virus nucleoprotein is preferentially localized in the cytoplasm and its immunization in mice shows higher immunogenicity and cross-reactivity.

    abstract::Many influenza vaccines targeted to hemagglutinin (HA) show efficient immunogenicity for protecting subjects against influenza virus infection. Major antigenic changes to HA molecules can help influenza virus to develop resistance against HA-targeted vaccines. DNA vaccines encoding conserved antigens protect animals a...


    pub_type: 杂志文章


    authors: Ohba K,Yoshida S,Zahidunnabi Dewan M,Shimura H,Sakamaki N,Takeshita F,Yamamoto N,Okuda K

    更新日期:2007-05-22 00:00:00

  • Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP.

    abstract::Immunization with a combination of several virulence-associated proteins is one of the strategies of developing effective protein-based vaccines to enhance the protection against Streptococcus pneumoniae. In this study, we evaluated the protection effects against pneumococcal infection caused by S. pneumoniae TIGR4 in...


    pub_type: 杂志文章


    authors: Cao J,Chen D,Xu W,Chen T,Xu S,Luo J,Zhao Q,Liu B,Wang D,Zhang X,Shan Y,Yin Y

    更新日期:2007-06-28 00:00:00

  • Correlates of immunity for pneumococcal conjugate vaccines.

    abstract::The purpose of the NIAID/FDA joint workshop, "correlates of immunity for pneumococcal conjugate vaccines (PCVs)," was to discuss the present understanding of protective immunity against invasive pneumococcal disease and identify in vitro measures that may represent immunologic correlates in future clinical trials. Ani...


    pub_type: 杂志文章,评审


    authors: Lee LH,Frasch CE,Falk LA,Klein DL,Deal CD

    更新日期:2003-05-16 00:00:00

  • Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge.

    abstract::Strains of Newcastle disease virus (NDV) can be separated into genotypes based on genome differences even though they are antigenically considered to be of a single serotype. It is widely recognized that an efficacious Newcastle disease (ND) vaccine made with any NDV does induce protection against morbidity and mortal...


    pub_type: 杂志文章


    authors: Miller PJ,King DJ,Afonso CL,Suarez DL

    更新日期:2007-10-10 00:00:00

  • Immunisation of a cohort Pacific children living in New Zealand over the first 2 years of life.

    abstract::Pacific children have had consistently evidenced low rates for routine childhood immunisations. Using the Pacific Islands Families: First 2 Years of Life cohort study, we investigated the immunisation rate of Pacific infants residing in New Zealand at 24-months postpartum. Full immunisation was reported for 89% of inf...


    pub_type: 杂志文章


    authors: Paterson J,Schluter P,Percival T,Carter S

    更新日期:2006-05-29 00:00:00

  • Genome-based approaches to develop vaccines against bacterial pathogens.

    abstract::Bacterial infectious diseases remain the single most important threat to health worldwide. Although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed to provide efficacious solutions against many others. The advent of whole-genome sequencing changed the ...


    pub_type: 杂志文章,评审


    authors: Serruto D,Serino L,Masignani V,Pizza M

    更新日期:2009-05-26 00:00:00

  • Immunogenicity, safety, biodistribution and persistence of ADVAX, a prophylactic DNA vaccine for HIV-1, delivered by in vivo electroporation.

    abstract::ADVAX is a DNA-based candidate HIV vaccine that was safe but weakly immunogenic when delivered intramuscularly (IM) in humans. Studies were performed in animal models to determine whether an alternative delivery method, in vivo electroporation (EP), could improve the immunogenicity of ADVAX while maintaining an accept...


    pub_type: 杂志文章


    authors: Dolter KE,Evans CF,Ellefsen B,Song J,Boente-Carrera M,Vittorino R,Rosenberg TJ,Hannaman D,Vasan S

    更新日期:2011-01-17 00:00:00

  • Preclinical evidence for implementing a prime-boost vaccine strategy for tuberculosis.

    abstract::In this review, published peer-reviewed preclinical studies using prime-boost tuberculosis (TB) vaccine regimens in animal challenge models for tuberculosis have been evaluated. These studies have been divided into groups that describe prime-boost vaccine combinations that performed better than, equivalent to, or wors...


    pub_type: 杂志文章,评审


    authors: Brennan MJ,Clagett B,Fitzgerald H,Chen V,Williams A,Izzo AA,Barker LF

    更新日期:2012-04-16 00:00:00

  • Crossreactive neutralizing antibodies induced by immunization with caprine arthritis-encephalitis virus surface glycoprotein.

    abstract::Four Saanen goats were immunized with affinity purified gp135 surface glycoprotein (SU) of caprine arthritis-encephalitis virus isolate 79-63 (CAEV-63) and evaluated for homologous and crossreactive serum neutralizing antibodies. CAEV-63 neutralizing antibodies were detected in all goats after seven immunizations with...


    pub_type: 杂志文章


    authors: Kemp RK,Knowles DP,Perry LL,McGuire TC,Besser TE,Cheevers WP

    更新日期:2000-01-18 00:00:00

  • Low varicella-related consultation rate in the Netherlands in primary care data.

    abstract:BACKGROUND:In the Netherlands, a relatively low varicella disease burden compared to other European countries is observed within routine surveillance. To validate this, we estimated the varicella-related consultation rate using The Integrated Primary Care Information database. METHODS:In this retrospective cohort stud...


    pub_type: 杂志文章


    authors: van Lier A,van Erp J,Donker GA,van der Maas NA,Sturkenboom MC,de Melker HE

    更新日期:2014-06-12 00:00:00

  • Head-to-head comparison on the immunogenicity of two HIV/AIDS vaccine candidates based on the attenuated poxvirus strains MVA and NYVAC co-expressing in a single locus the HIV-1BX08 gp120 and HIV-1(IIIB) Gag-Pol-Nef proteins of clade B.

    abstract::In this investigation we have generated and defined the immunogenicity of two novel HIV/AIDS vaccine candidates based on the highly attenuated vaccinia virus strains, MVA and NYVAC, efficiently expressing in the same locus (TK) and under the same viral promoter the codon optimized HIV-1 genes encoding gp120 and Gag-Po...


    pub_type: 杂志文章


    authors: Gómez CE,Nájera JL,Jiménez EP,Jiménez V,Wagner R,Graf M,Frachette MJ,Liljeström P,Pantaleo G,Esteban M

    更新日期:2007-04-12 00:00:00

  • Analysis of efficacy of CVD 103-HgR live oral cholera vaccine against all-cause travellers' diarrhoea in a randomised, double-blind, placebo-controlled study.

    abstract::Enterotoxigenic Escherichia coli (ETEC), which produces heat labile toxin (LT) and/or heat stable toxin (ST), is considered to be the most common known cause of travellers' diarrhoea (TD). Owing to the antigenic similarity between cholera toxin and LT, immunization with inactivated oral B-subunit/whole-cell cholera va...


    pub_type: 杂志文章,随机对照试验


    authors: Leyten EM,Soonawala D,Schultsz C,Herzog C,Ligthelm RJ,Wijnands S,Visser LG

    更新日期:2005-10-17 00:00:00

  • Safety and immunogenicity of a Vi-DT typhoid conjugate vaccine: Phase I trial in Healthy Filipino adults and children.

    abstract:BACKGROUND:Typhoid fever remains a major public health problem in low- and middle-income countries where children aged 2-14 years bear the greatest burden. Vi polysaccharide is poorly immunogenic in children <2 years of age, and protection in adults is modest. The limitations of Vi polysaccharide vaccines can be overco...


    pub_type: 杂志文章,随机对照试验


    authors: Capeding MR,Teshome S,Saluja T,Syed KA,Kim DR,Park JY,Yang JS,Kim YH,Park J,Jo SK,Chon Y,Kothari S,Yang SY,Ham DS,Ryu JH,Hwang HS,Mun JH,Lynch JA,Kim JH,Kim H,Excler JL,Sahastrabuddhe S

    更新日期:2018-06-18 00:00:00

  • Influenza vaccine effectiveness against influenza-associated hospitalization in 2015/16 season, Beijing, China.

    abstract:BACKGROUND:Vaccination is recommended to prevent influenza virus infection and associated complications. This study aimed to estimate the influenza vaccine effectiveness (VE) against hospitalization in the 2015/16 season in Beijing. METHODS:Patients who were hospitalized in the 5 study hospitals between 1 Oct 2015 and...


    pub_type: 杂志文章


    authors: Zhang Y,Wu P,Feng L,Yang P,Pan Y,Feng S,Qin Y,Zheng J,Puig-Barberà J,Muscatello D,MacIntyre R,Cowling BJ,Yu H,Wang Q

    更新日期:2017-05-25 00:00:00

  • Response to hepatitis-B vaccination in healthy homosexual individuals: retrospective case control study.

    abstract::The aim of the study was to compare the responses of homosexual men (HM) receiving a standard course of recombinant hepatitis B vaccine with those of healthcare workers vaccinated in the same hospital over the same period. Boosters for inadequate responders and repeat courses for non-responders were given. Forty of 61...


    pub_type: 杂志文章


    authors: Das S,Brassington M,Drake SM,Boxall E

    更新日期:2003-09-08 00:00:00

  • Bordetella pertussis iron regulated proteins as potential vaccine components.

    abstract::Bordetella pertussis is the etiologic agent of whooping cough, an illness whose incidence has been increasing over the last decades. Pertussis reemergence despite high vaccination coverage, together with the recent isolation of circulating strains deficient in some of the vaccine antigens, highlight the need for new v...


    pub_type: 杂志文章


    authors: Alvarez Hayes J,Erben E,Lamberti Y,Principi G,Maschi F,Ayala M,Rodriguez ME

    更新日期:2013-08-02 00:00:00

  • Transmissibility, infectivity and immunogenicity of a live human parainfluenza type 3 virus vaccine (HPIV3cp45) among susceptible infants and toddlers.

    abstract:BACKGROUND:This study examined the transmissibility between young children of an intranasally administered live attenuated human parainfluenza virus type 3 (HPIV3)-cp45 vaccine candidate. METHODS:Eighty subjects were enrolled in playgroups among whom there was at least one infected vaccinee in close contact with a ser...


    pub_type: 杂志文章,随机对照试验


    authors: Madhi SA,Cutland C,Zhu Y,Hackell JG,Newman F,Blackburn N,Murphy BR,Belshe RB,Karron RA,Deatly AM,Gruber WC,Bernstein DI,Wright PF

    更新日期:2006-03-20 00:00:00

  • Protection against diverse highly pathogenic H5 avian influenza viruses in chickens immunized with a recombinant fowlpox vaccine containing an H5 avian influenza hemagglutinin gene insert.

    abstract::A recombinant fowlpox vaccine with an H5 hemagglutinin gene insert protected chickens against clinical signs and death following challenge by nine different highly pathogenic H5 avian influenza viruses. The challenge viruses had 87.3 to 100% deduced hemagglutinin amino acid sequence similarity with the recombinant vac...


    pub_type: 杂志文章


    authors: Swayne DE,Garcia M,Beck JR,Kinney N,Suarez DL

    更新日期:2000-01-06 00:00:00

  • Self-administration of intranasal influenza vaccine: Immunogenicity and volunteer acceptance.

    abstract:BACKGROUND:In outbreak settings, mass vaccination strategies could maximize health protection of military personnel. Self-administration of live attenuated influenza vaccine (LAIV) may be a means to vaccinate large numbers of people and achieve deployment readiness while sparing the use of human resources. METHODS:A p...


    pub_type: 杂志文章,随机对照试验


    authors: Burgess TH,Murray CK,Bavaro MF,Landrum ML,O'Bryan TA,Rosas JG,Cammarata SM,Martin NJ,Ewing D,Raviprakash K,Mor D,Zell ER,Wilkins KJ,Millar EV

    更新日期:2015-07-31 00:00:00

  • Successful DNA immunisation of rats against fasciolosis.

    abstract::The liver fluke Fasciola hepatica contributes to great economic and health losses in the cattle industry in many countries, including Poland. Unfortunately, no vaccine against fasciolosis is commercially available. We have designed a DNA vaccine and tested it in rats. Groups of male or female rats received one intramu...


    pub_type: 杂志文章


    authors: Kofta W,Mieszczanek J,Płucienniczak G,Wedrychowicz H

    更新日期:2000-07-01 00:00:00

  • Characterisation of the antibody response to a totally synthetic immunocontraceptive peptide vaccine based on LHRH.

    abstract::In this study we describe our attempts to improve the immunogenicity of a synthetic epitope-based vaccine. The vaccine consists of an epitope (P25) that is recognised by CD4+ helper T cells and the target epitope luteinising hormone releasing hormone (LHRH). We show that replacement of the single cysteine residue with...


    pub_type: 杂志文章


    authors: Zeng W,Gauci S,Ghosh S,Walker J,Jackson DC

    更新日期:2005-08-15 00:00:00

  • Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin from Escherichia coli.

    abstract::Using a fixed dose of antigen, the immune response to detoxified mutants of LT-WT following intranasal (i.n.), subcutaneous (s.c.) and oral (i.g.) immunisation has been studied. When given i.n., both LT-WT and mutant toxin, K63, generated significant levels of toxin-specific IgG in the serum, and the levels of IgA in ...


    pub_type: 杂志文章


    authors: Douce G,Giuliani MM,Giannelli V,Pizza MG,Rappuoli R,Dougan G

    更新日期:1998-07-01 00:00:00

  • Effects of a multi-faceted program to increase influenza vaccine uptake among health care workers in nursing homes: A cluster randomised controlled trial.

    abstract::Despite the recommendation of the Dutch association of nursing home physicians (NVVA) to be immunized against influenza, vaccine uptake among HCWs in nursing homes remains unacceptably low. Therefore we conducted a cluster randomised controlled trial among 33 Dutch nursing homes to assess the effects of a systematical...


    pub_type: 杂志文章,随机对照试验


    authors: Looijmans-van den Akker I,van Delden JJ,Verheij TJ,van der Sande MA,van Essen GA,Riphagen-Dalhuisen J,Hulscher ME,Hak E

    更新日期:2010-07-12 00:00:00

  • Comparison of hemagglutination inhibition and microbead array assays for the measurement of influenza antibody levels in HIV-infected adults.

    abstract:INTRODUCTION:Determining vaccine responsiveness is challenging in immune compromised individuals. The microbead array (MBA) assay is a rapid, inexpensive test for evaluating influenza vaccine immunogenicity. MBA performance was compared to hemagglutination inhibition assay (HAI) utilizing HIV seropositive vaccine recip...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Thorne A,Chowdhury F,Singer J,Keynan Y,Fowke KR,Cooper C,CIHR Canadian HIV Trials Network (CTN) Influenza Vaccine Research Group.

    更新日期:2016-06-30 00:00:00

  • Cross-reactive immune responses in mice after genetic vaccination with cDNA encoding hantavirus nucleocapsid proteins.

    abstract::Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS) in about 150,000 individuals in Eurasia, and several hundred cases of hantavirus pulmonary syndrome (HPS) on the American continent annually. There is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. In this stu...


    pub_type: 杂志文章


    authors: Lindkvist M,Lahti K,Lilliehöök B,Holmström A,Ahlm C,Bucht G

    更新日期:2007-02-19 00:00:00

  • Protection against tetanus toxin after intragastric administration of two recombinant lactic acid bacteria: impact of strain viability and in vivo persistence.

    abstract::Non-pathogenic lactic acid bacteria (LAB) are attractive as live carriers to deliver protective antigens to the mucosal immune system. Both persisting and non-persisting strains of lactic acid bacteria have been evaluated and seem to work equally well by the systemic and nasal routes of administration. However, it is ...


    pub_type: 杂志文章


    authors: Grangette C,Müller-Alouf H,Geoffroy M,Goudercourt D,Turneer M,Mercenier A

    更新日期:2002-09-10 00:00:00

  • Colinear synthesis of an antigen-specific B-cell epitope with a 'promiscuous' tetanus toxin T-cell epitope: a synthetic peptide immunocontraceptive.

    abstract::Carrier conjugation is commonly used to provide T-cell help for small, linear peptides containing antigen-specific B-cell epitopes. However, carrier conjugation is expensive, variable and often results in adverse side effects if the conjugate is administered repeatedly. To eliminate the need for carrier conjugation, w...


    pub_type: 杂志文章


    authors: O'Hern PA,Liang ZG,Bambra CS,Goldberg E

    更新日期:1997-11-01 00:00:00

  • Safety of Russian-backbone seasonal trivalent, live-attenuated influenza vaccine in a phase II randomized placebo-controlled clinical trial among children in urban Bangladesh.

    abstract:INTRODUCTION:Live-attenuated influenza vaccines (LAIVs) have the potential to be affordable, effective, and logistically feasible for immunization of children in low-resource settings. MATERIAL AND METHODS:We conducted a phase II, randomized, double-blind, parallel group, placebo-controlled trial on the safety of the ...


    pub_type: 杂志文章,随机对照试验


    authors: Ortiz JR,Goswami D,Lewis KD,Sharmeen AT,Ahmed M,Rahman M,Rahman MZ,Feser J,Neuzil KM,Brooks WA

    更新日期:2015-06-26 00:00:00

  • In ovo administration of Toll-like receptor ligands encapsulated in PLGA nanoparticles impede tumor development in chickens infected with Marek's disease virus.

    abstract::One of the economically important diseases in the poultry industry is Marek's disease (MD) which is caused by Marek's disease virus (MDV). The use of current vaccines provides protection against clinical signs of MD in chickens. However, these vaccines do not prevent the transmission of MDV to susceptible hosts, hence...


    pub_type: 杂志文章


    authors: Bavananthasivam J,Alkie TN,Astill J,Abdul-Careem MF,Wootton SK,Behboudi S,Yitbarek A,Sharif S

    更新日期:2018-06-27 00:00:00