当前位置： SCI文献检索 > VACCINE期刊下所有文献 > A novel lamprey antibody sequence to multimerize and increase the immunogenicity of recombinant viral and bacterial vaccine antigens.

A novel lamprey antibody sequence to multimerize and increase the immunogenicity of recombinant viral and bacterial vaccine antigens.

Abstract：

:Hemagglutinin, the major surface protein of influenza viruses, was recombinantly expressed in eukaryotic cells as a monomer instead of its native trimer, and was only immunogenic when administered with an adjuvant [Pion et al. 2014]. In order to multimerize this antigen to increase its immunogenicity, a cysteine-rich peptide sequence found at the extreme C-terminus of lamprey variable lymphocyte receptor (VLR)-B antibodies was fused to various recombinant hemagglutinin (rHA) proteins from A and B influenza virus strains. The rHA-Lamp fusion (rHA fused to the lamprey sequence) protein was expressed in Leishmania tarentolae and Chinese hamster ovary (CHO) cells and shown to produce several multimeric forms. The multimers produced were very stable and more immunogenic in mice than monomeric rHA. The lamprey VLR-B sequence was also used to multimerize the neuraminidase (NA) of influenza viruses expressed in CHO cells. For some viral strains, the NA was expressed as a tetramer like the native viral NA form. In addition, the lamprey VLR-B sequence was fused with two surface antigens of Shigella flexneri 2a, the invasion plasmid antigen D and a double mutated soluble form of the membrane expression of the invasion plasmid antigen H namely MxiH. The fusion proteins were expressed in Escherichia coli to produce the respective multimer protein forms. The resulting proteins had similar multimeric forms as rHA-Lamp protein and were more immunogenic in mice than the monomer forms. In conclusion, the VLR-B sequence can be used to increase the immunogenicity of recombinant viral and bacterial antigens, thus negating the need for adjuvants.

journal_name

Vaccine

journal_title

Vaccine

authors

Peubez I,Margot S,Buffin S,Pion C,Bernard MC,Dinadayala P,Poncet D,Deloire S,Marco S,Legastelois I

doi

10.1016/j.vaccine.2020.10.073

subject

Has Abstract

pub_date

2020-11-25 00:00:00

pages

7905-7915

issue

50

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(20)31393-1

journal_volume

38

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Temporal analysis of the bovine lymph node transcriptome during cattle tick (Rhipicephalus microplus) infestation.

    abstract：:Livestock production is a fundamental source of revenue and nutrition, wherein cattle-farming constitutes one of the major agricultural industries. Vectors and vector-borne diseases constitute one of the major factors that decrease the livelihood of all farming communities, more so in resource-poor communities and dev...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.08.060

    authors： Robbertse L,Richards SA,Stutzer C,Olivier NA,Leisewitz AL,Crafford JE,Maritz-Olivier C

    更新日期：2020-10-14 00:00:00

  • Reactions to small pox vaccine in naïve and previously-vaccinated individuals.

    abstract：:Two hundred health care workers in England and Wales were vaccinated with the Lister/Elstree strain of the vaccinia virus, and completed health diaries for 21 days or until the lesion had scabbed over. Pain and temperature were measured daily, and all other symptoms recorded freehand by the vaccinee. One hundred and f...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.10.052

    authors： Auckland C,Cowlishaw A,Morgan D,Miller E

    更新日期：2005-07-14 00:00:00

  • Immunogenicity and efficacy of two candidate human metapneumovirus vaccines in cynomolgus macaques.

    abstract：:Human metapneumovirus (HMPV) is an important cause of acute respiratory tract disease for which the development of vaccine candidates is warranted. We have previously described the generation of an iscom matrix-adjuvanted HMPV fusion protein subunit vaccine (Fsol) and a live-attenuated vaccine (HMPVM11). Here, we eval...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.05.052

    authors： Herfst S,Schrauwen EJ,de Graaf M,van Amerongen G,van den Hoogen BG,de Swart RL,Osterhaus AD,Fouchier RA

    更新日期：2008-08-05 00:00:00

  • Impact of heptavalent pneumococcal conjugate vaccine on invasive disease, antimicrobial resistance and colonization in Alaska Natives: progress towards elimination of a health disparity.

    abstract：:We evaluated invasive pneumococcal disease (IPD), antimicrobial resistance and nasopharyngeal colonization before and after introduction of pneumococcal conjugate vaccine (PCV7) in Alaska Natives (AN), a population with high IPD rates. We obtained IPD rates from population-based surveillance. Colonization was determin...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.08.100

    authors： Hennessy TW,Singleton RJ,Bulkow LR,Bruden DL,Hurlburt DA,Parks D,Moore M,Parkinson AJ,Schuchat A,Butler JC

    更新日期：2005-12-01 00:00:00

  • Is varicella vaccination associated with pediatric arterial ischemic stroke? A population-based cohort study.

    abstract：BACKGROUND AND PURPOSE:Varicella disease is a risk factor for pediatric Arterial Ischemic Stroke (AIS). Isolated case reports have emerged suggesting that varicella vaccination may also pose a risk for AIS. METHODS:This retrospective population-based cohort study assessed the risk of AIS in children who received a var...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.04.012

    authors： MacDonald SE,Dover DC,Hill MD,Kirton A,Simmonds KA,Svenson LW

    更新日期：2018-05-11 00:00:00

  • Hyporesponsiveness to re-challenge dose following pneumococcal polysaccharide vaccine at 12 months of age, a randomized controlled trial.

    abstract：BACKGROUND:To evaluate the immunological impact of the 23-valent pneumococcal polysaccharide vaccine (23vPPS) at 12 months, for children who have received zero to three infant doses of seven-valent pneumococcal conjugate vaccine (PCV), on responses to a subsequent exposure to a small dose of 23vPPS (mPPS). METHODS:Fiv...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2010.02.087

    authors： Russell FM,Carapetis JR,Balloch A,Licciardi PV,Jenney AW,Tikoduadua L,Waqatakirewa L,Pryor J,Nelson J,Byrnes GB,Cheung YB,Tang ML,Mulholland EK

    更新日期：2010-04-26 00:00:00

  • The effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type 1.

    abstract：:We investigated whether pre-existing adenovirus-specific immunity influenced the development of immunity to a foreign antigen expressed by recombinant adenovirus. Active adenovirus-specific immunity was induced in cotton rats by i.n. administration of wild type human adenovirus type 5 (HAd5) two weeks before immunisat...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00279-5

    authors： Papp Z,Babiuk LA,Baca-Estrada ME

    更新日期：1999-02-26 00:00:00

  • Human papillomavirus (HPV) vaccination and subsequent sexual behaviour: evidence from a large survey of Nordic women.

    abstract：OBJECTIVE:To assess whether recipients and non-recipients of the human papillomavirus (HPV) vaccine subsequently differ in terms of sexual risk taking behaviour. DESIGN:Cross-sectional survey. Sequential analyses constructed from self-reported age at vaccination, age at first intercourse and age at response. SETTING:...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.07.025

    authors： Hansen BT,Kjær SK,Arnheim-Dahlström L,Liaw KL,Jensen KE,Thomsen LT,Munk C,Nygård M

    更新日期：2014-09-03 00:00:00

  • Homotypic and heterotypic immune responses to group A rotaviruses in parenterally immunized sheep.

    abstract：:Immune responses to human rotaviruses were investigated in sheep with a view to obtaining antibodies for passive immunotherapy of humans. Eighteen adult sheep with previous natural exposure to rotavirus serotypes G3 and G6 were immunized parenterally with purified preparations of either individual rotavirus serotypes ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(93)90027-u

    authors： Beards GM,King JA,Mazhar S,Landon J,Desselberger U

    更新日期：1993-01-01 00:00:00

  • Assessing potential introduction of universal or targeted hepatitis A vaccination in the Netherlands.

    abstract：:In many industrialized countries, hepatitis A incidence rates have declined steadily in the past decades. Since future cohorts of non-vaccinated elderly will lack protection against disease and the burden of hepatitis A is higher with increasing age, this could be an argument in favour of taking preventive measures su...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.06.014

    authors： Suijkerbuijk AW,Lugnér AK,van Pelt W,Wallinga J,Verhoef LP,de Melker HE,de Wit GA

    更新日期：2012-07-27 00:00:00

  • A recombinant hybrid outer membrane protein for vaccination against Pseudomonas aeruginosa.

    abstract：:Among the numerous targets which can be used for the development of vaccines against Pseudomonas aeruginosa we focused on the outer membrane proteins OprF and OprI. The C-terminal part of OprF from aa 190 to aa 350 was investigated for its conservation and its localization of B-cell epitopes. A hybrid protein which co...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00420-4

    authors： Knapp B,Hundt E,Lenz U,Hungerer KD,Gabelsberger J,Domdey H,Mansouri E,Li Y,von Specht BU

    更新日期：1999-03-26 00:00:00

  • Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep.

    abstract：:Positive clone Hq05 of Haemaphysalis qinghaiensis (named following those cDNA clones cloned from the tick before) was obtained by differentially screening of a cDNA library of the tick with rabbit anti-tick salivary gland serum and rabbit anti-tick saliva serum. Hq05 contains an open reading frame (ORF) of 540bp that ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.10.067

    authors： Gao J,Luo J,Fan R,Schulte-Spechtel UC,Fingerle V,Guan G,Zhao H,Li Y,Ren Q,Ma M,Liu Z,Liu A,Dang Z,Sugimoto C,Yin H

    更新日期：2009-01-14 00:00:00

  • Seroprevalence of pertussis amongst healthcare professionals in Spain.

    abstract：INTRODUCTION:This multi-center, hospital-based observational study determined the seroprevalence of pertussis antibodies amongst healthcare professionals from three different hospitals in Spain to ascertain the health status of professionals attending to susceptible groups who are at risk of contracting and transmittin...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vaccine.2015.12.036

    authors： Rodríguez de la Pinta ML,Castro Lareo MI,Ramon Torrell JM,García de Lomas J,Devadiga R,Reyes J,McCoig C,Tafalla M,García-Corbeira P

    更新日期：2016-02-17 00:00:00

  • Factors influencing the antibody response of dogs vaccinated against rabies.

    abstract：:Since 2000, there has been a legal requirement in the UK that dogs and cats should have an effective rabies vaccination with demonstrable sero-conversion if their owners wish to avoid quarantine on re-entry to the UK. In 2002, 10,483 rabies titres were determined on dogs at the VLA. Statistical analyses assessed the e...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.10.015

    authors： Kennedy LJ,Lunt M,Barnes A,McElhinney L,Fooks AR,Baxter DN,Ollier WE

    更新日期：2007-12-12 00:00:00

  • Epitope polarity and adjuvants influence the fine specificity of the humoral response against Semliki Forest virus specific peptide vaccines.

    abstract：:The humoral response to synthetic peptide vaccines against Semliki Forest virus (SFV) in H-2d BALB/c mice was investigated with the enzyme linked immunosorbent assay and the pepscan technique. The peptide vaccines consisted of amino acid sequences 240-255 (B) and 137-151 (T) of the E2 membrane protein of SFV colinearl...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00038-3

    authors： Fernández IM,Harmsen M,Benaissa-Trouw BJ,Stuij I,Puyk W,Meloen RH,Snippe H,Kraaijeveld CA

    更新日期：1998-10-01 00:00:00

  • Analysis of factors influencing vaccine uptake: perspectives from Belgium.

    abstract：METHODS AND OBJECTIVES:To estimate the infant vaccination coverage in Belgium, a random cluster sample according to the expanded program on immunization (EPI) cluster sampling technique was performed in 1999 in the Flemish (Flanders) and French (Wallonia) speaking community of Belgium. The objective was to document the...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(01)00307-3

    authors： Swennen B,Van Damme P,Vellinga A,Coppieters Y,Depoorter AM

    更新日期：2001-10-15 00:00:00

  • Protein-prime/modified vaccinia virus Ankara vector-boost vaccination overcomes tolerance in high-antigenemic HBV-transgenic mice.

    abstract：BACKGROUND:Therapeutic vaccination is a novel treatment approach for chronic hepatitis B, but only had limited success so far. We hypothesized that optimized vaccination schemes have increased immunogenicity, and aimed at increasing therapeutic hepatitis B vaccine efficacy. METHODS:Modified Vaccinia virus Ankara (MVA)...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.12.060

    authors： Backes S,Jäger C,Dembek CJ,Kosinska AD,Bauer T,Stephan AS,Dišlers A,Mutwiri G,Busch DH,Babiuk LA,Gasteiger G,Protzer U

    更新日期：2016-02-10 00:00:00

  • Immune responses in mice induced by prime-boost schemes of the Plasmodium falciparum apical membrane antigen 1 (PfAMA1)-based DNA, protein and recombinant modified vaccinia Ankara vaccines.

    abstract：:The apical membrane antigen 1 (AMA1) of malaria parasites is a leading vaccine candidate. Its expression in merozoites and sporozoites and its importance for erythrocyte and hepatocyte invasion underline the significance of both humoral and cellular immunities against this antigen in malaria protection. We have genera...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.05.099

    authors： Miao J,Li X,Liu Z,Xue C,Bujard H,Cui L

    更新日期：2006-09-11 00:00:00

  • Convening on the influenza human viral challenge model for universal influenza vaccines, Part 2: Methodologic considerations.

    abstract：:In response to global interest in the development of a universal influenza vaccine, the Bill & Melinda Gates Foundation, PATH, and the Global Funders Consortium for Universal Influenza Vaccine Development convened a meeting of experts (London, UK, May 2018) to assess the role of a standardized controlled human influen...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.06.053

    authors： Innis BL,Scorza FB,Blum JS,Jain VK,Aguilar AO,Post DJ,Roberts PC,Wairagkar N,White J,Bresee J

    更新日期：2019-08-14 00:00:00

  • Sub-optimal prevalence of mumps antibodies in a population based study of young adults in Israel after 20 years of two dose universal vaccination policy.

    abstract：BACKGROUND:A recent mumps outbreak in Israel despite an ongoing national program of a 2-dose universal childhood vaccination policy since 1988, raised questions regarding population immunity among young adults. OBJECTIVE:To assess the seroprevalence of mumps antibodies among young Israeli adults born after 1987 in ord...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.01.103

    authors： Levine H,Ankol OE,Rozhavski V,Davidovitch N,Aboudy Y,Zarka S,Balicer RD

    更新日期：2011-03-24 00:00:00

  • Vaccine decision-making begins in pregnancy: Correlation between vaccine concerns, intentions and maternal vaccination with subsequent childhood vaccine uptake.

    abstract：INTRODUCTION:Maternal and childhood vaccine decision-making begins prenatally. Amongst pregnant Australian women we aimed to ascertain vaccine information received, maternal immunisation uptake and attitudes and concerns regarding childhood vaccination. We also aimed to determine any correlation between a) intentions a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.08.003

    authors： Danchin MH,Costa-Pinto J,Attwell K,Willaby H,Wiley K,Hoq M,Leask J,Perrett KP,O'Keefe J,Giles ML,Marshall H

    更新日期：2018-10-22 00:00:00

  • A short report on the highlights of world-wide development of RIX4414: a North American experience comparative evaluation of safety and immunogenicity of two dosages of an oral live attenuated human rotavirus vaccine (RIX4414) in infants in the United Sta

    abstract：:This randomized, double-blind trial conducted in the US and Canada was conducted to compare the safety and immunogenicity of two dosages of live attenuated oral monovalent G1 human rotavirus (HRV) vaccine administered orally at a 2-month interval concomitantly with routine childhood vaccines to healthy infants 5-15 we...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2005.07.030

    authors： Dennehy PH,North American Human Rotavirus Vaccine Study Group.

    更新日期：2006-05-01 00:00:00

  • The defect in the metabolism of erythritol of the Brucella abortus B19 vaccine strain is unrelated with its attenuated virulence in mice.

    abstract：:The role of the defect in erythritol catabolism in the attenuated virulence of Brucella abortus B19 vaccine strain in mice was investigated by means of five different strains: (i) the erythritol sensitive B19 vaccine strain; (ii) a natural erythritol tolerant (NET) mutant obtained spontaneously from B19; (iii) an eryt...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)00063-2

    authors： Sangari FJ,Grilló MJ,Jiménez De Bagüés MP,González-Carreró MI,García-Lobo JM,Blasco JM,Agüero J

    更新日期：1998-10-01 00:00:00

  • Advax™, a novel microcrystalline polysaccharide particle engineered from delta inulin, provides robust adjuvant potency together with tolerability and safety.

    abstract：:There is an ongoing need for new adjuvants to facilitate development of vaccines against HIV, tuberculosis, malaria and cancer, amongst many others. Unfortunately, the most potent adjuvants are often associated with toxicity and safety issues. Inulin, a plant-derived polysaccharide, has no immunological activity in it...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2015.09.030

    authors： Petrovsky N,Cooper PD

    更新日期：2015-11-04 00:00:00

  • Process development for the mass production of Ehrlichia ruminantium.

    abstract：:This work describes the optimization of a cost-effective process for the production of an inactivated bacterial vaccine against heartwater and the first attempt to produce the causative agent of this disease, the rickettsia Ehrlichia ruminantium (ER), using stirred tanks. In vitro, it is possible to produce ER using c...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.08.109

    authors： Marcelino I,Sousa MF,Veríssimo C,Cunha AE,Carrondo MJ,Alves PM

    更新日期：2006-03-06 00:00:00

  • Safety and immunogenicity of an 8 year interval heterologous prime-boost influenza A/H7N7-H7N9 vaccination.

    abstract：BACKGROUND:Influenza A/H7N9 viruses are undergoing antigenic drift since their emergence in 2013, and vaccination strategies are needed for pandemic preparedness. Two doses of adjuvanted monovalent inactivated influenza A/H7N9 vaccine (IIV1 A/H7N9) are needed for optimal serological responses. However, administering 2 ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.03.071

    authors： El Sahly HM,Atmar RL,Patel SM,Bellamy A,Liu L,Hong W,Zhu H,Guan Y,Keitel WA,DMID 13-0033 Vaccine Study Group.

    更新日期：2019-05-01 00:00:00

  • Analogues of muramyl dipeptide (MDP) and tuftsin limit infection and inflammation in murine model of sepsis.

    abstract：:Pharmacological manipulation of the balance between pro- and anti-inflammatory mediators emerges as a key aspect of a successful treatment of sepsis. A murine model of septic shock was developed and chosen conjugates (1a, 1b, 8a, 8c) and analogs (T2) of muramyl dipeptide and tuftsin were tested in this model as prospe...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.11.017

    authors： Wardowska A,Dzierzbicka K,Szaryńska M,Dabrowska-Szponar M,Wiśniewska K,Myśliwski A,Trzonkowski P

    更新日期：2009-01-14 00:00:00

  • Public opinion on childhood immunisations in Iceland.

    abstract：INTRODUCTION:In recent years, vaccine preventable diseases such as measles and pertussis have been re-emerging in Western countries, maybe because of decreasing participation in childhood vaccination programs in some countries. There is clear evidence for vaccine efficacy and the risk of adverse effects is low. This ne...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.10.125

    authors： Óskarsson Ý,Guðnason Þ,Jónsdóttir GA,Kristinsson KG,Briem H,Haraldsson Á

    更新日期：2015-12-16 00:00:00

  • Enhancing immune responses to inactivated porcine parvovirus oil emulsion vaccine by co-inoculating porcine transfer factor in mice.

    abstract：:Inactivated porcine parvovirus (PPV) vaccines are available commercially and widely used in the breeding herds. However, inactivated PPV vaccines have deficiencies in induction of specific cellular immune response. Transfer factor (TF) is a material that obtained from the leukocytes, and is a novel immune-stimulatory ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.05.077

    authors： Wang RN,Wang YB,Geng JW,Guo DH,Liu F,Chen HY,Zhang HY,Cui BA,Wei ZY

    更新日期：2012-07-27 00:00:00

  • Vaccination with recombinant Modified Vaccinia Ankara (MVA) viruses expressing single African horse sickness virus VP2 antigens induced cross-reactive virus neutralising antibodies (VNAb) in horses when administered in combination.

    abstract：:African horse sickness is a lethal viral disease of equids transmitted by biting midges of the Genus Culicoides. The disease is endemic to sub-Saharan Africa but outbreaks of high mortality and economic impact have occurred in the past in non-endemic regions of Africa, Asia and Southern Europe. Vaccination is critical...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.04.005

    authors： Manning NM,Bachanek-Bankowska K,Mertens PPC,Castillo-Olivares J

    更新日期：2017-10-20 00:00:00