Abstract:
:The three-dimensional structure of the saccharopine reductase enzyme from the budding yeast Saccharomyces cerevisiae was determined to 1.7-A resolution in the apo form by using molecular replacement. The enzyme monomer consists of three domains: domain I is a variant of the Rossmann fold, domain II folds into a alpha/beta structure containing a mixed seven-stranded beta-sheet as the central core, and domain III has an all-helical fold. Comparative fold alignment with the enzyme from Magnaporthe grisea suggests that domain I binds to NADPH, and domain II binds to saccharopine and is involved in dimer formation. Domain III is involved in closing the active site of the enzyme once substrates are bound. Structural comparison of the saccharopine reductase enzymes from S. cerevisiae and M. grisea indicates that domain II has the highest number of conserved residues, suggesting that it plays an important role in substrate binding and in spatially orienting domains I and III.
journal_name
Cell Biochem Biophysjournal_title
Cell biochemistry and biophysicsauthors
Andi B,Cook PF,West AHdoi
10.1385/CBB:46:1:17subject
Has Abstractpub_date
2006-01-01 00:00:00pages
17-26issue
1eissn
1085-9195issn
1559-0283pii
CBB:46:1:17journal_volume
46pub_type
杂志文章abstract::Active transport is essential for cellular function, while impaired transport has been linked to diseases such as neuronal degeneration. Much long distance transport in cells uses opposite polarity molecular motors of the kinesin and dynein families to move cargos along microtubules. It is clear that many types of car...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-008-9028-1
更新日期:2008-01-01 00:00:00
abstract::STAT3 pathway plays an important role in the growth of diffuse large B-cell lymphoma (DLBCL) cells. Here we investigated the antitumor activity of Quercetin, a flavonoid compound, in combination with rituximab in DLBCL cell lines in vitro. We found that Quercetin synergistically enhanced rituximab-induced growth inhib...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0064-8
更新日期:2014-11-01 00:00:00
abstract::H5N1 is a subtype of the influenza A virus that can cause disease in humans and many other animal species. Oseltamivir (Tamiflu) is a potent and selective antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase (NA), a flu protein responsible for the release and spread of the...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9709-2
更新日期:2014-03-01 00:00:00
abstract::The precise regulation of both the magnitude and the duration of Janus kinase (JAK) catalytic activity is essential for the cytokine orchestration of many biological processes, and the dysregulation of JAK activity has pathological implications. Immunosuppressive disease states, such as X-linked severe combined immuno...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:34:1:17
更新日期:2001-01-01 00:00:00
abstract::Curcumin is a yellow-orange dye widely used as a spice, food coloring and food preservative. It also exhibits a broad range of therapeutic effects against different disorders such as cancer, diabetes, or neurodegenerative diseases. As a compound insoluble in water curcumin accumulates in cell membranes and due to this...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-020-00906-5
更新日期:2020-06-01 00:00:00
abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0498-z
更新日期:2015-06-01 00:00:00
abstract::This study is aimed to investigate the efficacy and underlying the mechanism of propofol in treatment of ischemia reperfusion (IR)-induced lung injury in rats, providing a novel insight of therapeutic strategy for IR-induced lung injury. 120 healthy SD rats were selected and randomly divided into sham operation group,...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0088-0
更新日期:2014-12-01 00:00:00
abstract::The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0576-x
更新日期:2015-09-01 00:00:00
abstract::The CEP genes play a pivotal role in the replication of the cell. CEP family proteins form the major constituents of the centrosome and play a prominent role in centriole biogenesis and in cell replication. Alteration in CEP genes will result in disruption of cell cycle that may in turn cause cancer. In our study, we ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0153-8
更新日期:2014-12-01 00:00:00
abstract::Molecular motors are responsible of transporting a wide variety of cargos in the cytoplasm. Current efforts are oriented to characterize the biophysical properties of motors in cells with the aim of elucidating the mechanisms of these nanomachines in the complex cellular environment. In this study, we present an algor...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9397-3
更新日期:2013-01-01 00:00:00
abstract::The American Diabetes Association announces funding for clinical and basic science studies in three major areas of research that focus on cell-based insulin replacement in type 1 diabetes: 1. Genetic engineering of nonpancreatic cells into glucose-sensitive insulin-producing cells; 2. Transforming stem cells, pancreat...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:40:3s:023
更新日期:2004-01-01 00:00:00
abstract::Hepatocyte growth factor (HGF) has been known to enhance the growth of normal hepatocytes, but also to inhibit the growth of neoplastic cells. This article examines the involvement of HGF in the hepatocarcinogenesis caused by peroxisome proliferators (PPs). Up to 78 wk after male F-344 rats were orally given (4-chloro...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1385/cbb:32:1-3:221
更新日期:2000-01-01 00:00:00
abstract::Molecular dynamics simulations, binding free energy calculations, principle component analysis (PCA), and residue interaction network analysis were employed in order to investigate the molecular mechanism of M184I single mutation which played pivotal role in making the HIV-1 reverse transcriptase (RT) totally resistan...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-015-0709-2
更新日期:2016-03-01 00:00:00
abstract::The aim of the study was to investigate changes in the plasma antioxidative activity and in lipid peroxidation after administration of endothelin-1 (ET-1) and endothelin receptor blockers and additionally, to estimate blood pressure. The study was performed on male Wistar rats (n = 6 per group) divided into 4 groups w...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0371-0
更新日期:2015-04-01 00:00:00
abstract::This study explored the effect of a first aid model employing two nurses on the efficient rescue operation time and the efficient resuscitation time for major burn patients. A two-nurse model of first aid was designed for major burn patients. The model includes a division of labor between the first aid nurses and the ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0306-9
更新日期:2015-03-01 00:00:00
abstract::The phosphatidylinositol 3-kinase/AKT (PI3K/AKT) pathway plays a critical role in human cancer. We determined the expression patterns of class I PI3K catalytic subunits and evaluated their importance in the development or progression of colorectal cancer (CRC). For this purpose, expression of class I PI3K isoforms was...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9257-6
更新日期:2012-01-01 00:00:00
abstract::Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN with the E2 may evoke cellular signal transduction implicated in viral pathogenesis. We developed a cell model ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-009-9069-0
更新日期:2010-01-01 00:00:00
abstract::It has been long known that the oncogenic extracellular environment plays an indispensable role in developing and nurturing cancer cell progression and in resistance to standard treatments. However, by how much the biophysical components of tumour extracellular environment contribute to these processes is uncertain. I...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-020-00964-9
更新日期:2021-01-12 00:00:00
abstract::Either the role of the adaptive immune system or the interaction between innate and adaptive immune systems in familial Mediterranean fever (FMF) is not clear so far. So, we planned to search for the interaction between the innate and adaptive immune systems in the pathogenesis of FMF by investigating polymorphism for...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-012-9416-4
更新日期:2013-03-01 00:00:00
abstract::Coronary blood flow is tightly coupled to myocardial oxygen consumption to maintain a consistently high level of myocardial oxygen extraction. This tight coupling has been proposed to depend on periarteriolar oxygen tension, signals released from cardiomyocytes (adenosine acting on K+(ATP) channels), and/or the endoth...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1385/CBB:43:1:017
更新日期:2005-01-01 00:00:00
abstract::The airways in asthma and COPD are characterized by an increase in airway smooth muscle (ASM) mass and bronchial vascular changes associated with increased expression of pro-angiogenic growth factors, such as fibroblast growth factors (FGF-1 and FGF-2) and vascular endothelial growth factor (VEGF). We investigated the...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9331-0
更新日期:2013-11-01 00:00:00
abstract::Resveratrol (RSV) is known for its antioxidant properties; however, this compound has been proposed to have cytotoxic and pro-oxidant effects depending on its concentration and time of exposure. We previously reported the cell cycle arrest effect of low doses of RSV in GRX cells, an activated hepatic stellate cell mod...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9703-8
更新日期:2014-03-01 00:00:00
abstract::The possibility that binuclear dinitrosyl iron complexes with glutathione and cysteine (DNIC-GSН and B-DNIC-Cys) have a strong cytotoxic effect on the growth of endometrioid tumours (EMT) in rats with surgically induced experimental endometriosis established in our previous studies has been supported with experimental...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-019-00865-6
更新日期:2019-03-01 00:00:00
abstract::To explore the proteomic changes of placental trophoblastic cells in preeclampsia-eclampsia (PE), placental trophoblastic cells from normally pregnant women and women with hypertension during gestational period were prepared by laser capture microdissection (LCM), and proteins isolated from these cells were subjected ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-013-9792-4
更新日期:2014-06-01 00:00:00
abstract::The aim of the study was to explore the effects of different fitness sports on cognitive function and emotion of the aged people. A total of 150 subjects aged between 60 and 70 were recruited from Shenyang Aged University and elderly activity center. All subjects reported no fitness before this study. The aged subject...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-014-0100-8
更新日期:2014-12-01 00:00:00
abstract::Osteoclast is the only cell that can degrade bone tissue in vivo. Recent studies have shown the important role of cytoskeleton dynamics in osteolysis and the formation of podosome belt in osteoclasts. This process is regulated by the dynamic microtubule (MT) network. We treated osteoclast precursor cells Raw264.7 with...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-014-0258-0
更新日期:2015-03-01 00:00:00
abstract::This study was aimed to compare the outcomes of two operational methods used for the fixation of calcaneal fracture, the open reduction using a plate and the minimally invasive cannulated screw fixations. Thus, we attempted to find out as to which of these fixation regimens was therapeutically superior by assessing im...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12013-014-0436-0
更新日期:2015-05-01 00:00:00
abstract::A fast-track clinical pathway is designed to streamline patient care delivery and maximize cost effectiveness. It has decreased postoperative length of stay (LOS) and hospital charges for many surgical procedures. However, data on clinical pathways after liver surgery are sparse. This study examined whether use of a f...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-011-9203-7
更新日期:2011-11-01 00:00:00
abstract::To facilitate drug development for lung delivery, it is highly demanding to establish appropriate airway epithelial cell models as transport barriers to evaluate pharmacokinetic profiles of drug molecules. Besides the cancer-derived cell lines, as the primary cell model, normal human bronchial epithelial (NHBE) cells ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章
doi:10.1007/s12013-016-0719-8
更新日期:2016-06-01 00:00:00
abstract::Far from now are the days when investigators raced to identify the proteolytic system responsible for the degradation of their favorite protein. Nowadays, it is well accepted that a given protein can be degraded by different systems depending on factors such as cell type, cellular conditions, or functionality of each ...
journal_title:Cell biochemistry and biophysics
pub_type: 杂志文章,评审
doi:10.1007/s12013-013-9623-7
更新日期:2013-09-01 00:00:00