Establishment and characterization of 20 human non-small cell lung cancer cell lines in a serum-free defined medium (ACL-4).

Abstract:

:To facilitate the studies in cell biology and drug sensitivity of non-small cell lung cancer, cell samples from 55 patients have been used to establish cell lines in culture using a chemically defined medium (ACL-4). A total of 20 cell lines (36 percent) were directly established and characterized: 14 (44 percent) from pleural effusions, five (29 percent) from resected primary tumors, and one (25 percent) from ascitic fluids. They comprised 16 adenocarcinoma, two large cell carcinoma, one squamous cell carcinoma, and one malignant mesothelioma. Each cell line had distinct gross morphologic features and population doubling times from 21 to 75 h. The plating efficiency was 0.01 to 9.51 percent. The modal chromosome number varied from 45 to 108. Secretion of tumor markers (carcinoembryonic antigen, sialyl Lewis Xi, CA 50, CA 125, and CA 19-9) into the medium was also different in each cell line. Most of the cell lines have been xenografted into nude mice and found to be tumorigenic. Survival of 20 patients whose tumor cell specimens continually grew in culture at any time during their clinical course was significantly shorter than that of 35 patients with no in vitro tumour growth (median survival time of 28 weeks vs 53 weeks, p = 0.0093). Our study indicates that in vitro tumor cell growth appears to be an adverse prognostic factor in patients with non-small cell lung cancer.

journal_name

Chest

journal_title

Chest

authors

Masuda N,Fukuoka M,Takada M,Kudoh S,Kusunoki Y

doi

10.1378/chest.100.2.429

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

429-38

issue

2

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(16)37176-8

journal_volume

100

pub_type

杂志文章

相关文献

CHEST文献大全
  • Successful Healing of Tracheal Radionecrosis: Role of Hyperbaric Oxygen Therapy.

    abstract::Hyperbaric oxygen therapy, the administration of 100% oxygen at pressures > 1 atm, is believed to promote wound healing by increasing angiogenesis and collagen synthesis. To our knowledge, this treatment modality has never been described in patients with tracheal radionecrosis. Here, we report the case of a 55-year-ol...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1016/j.chest.2016.03.027

    authors: Ariza-Prota M,Morales A,Grajeda J,López-Lisbona R,Cubero N,Dorca J,Rosell A

    更新日期:2016-12-01 00:00:00

  • Changes in cerebral oxygenation and hemodynamics during obstructive sleep apneas.

    abstract:OBJECTIVE:To evaluate changes in cerebral tissue oxygenation and blood volume during obstructive sleep apnea (OSA). METHODS:We studied eight men with moderate to severe OSA by near-infrared spectroscopy (NIRS) simultaneously with polysomnography during nocturnal sleep (five patients) and daytime naps (three). RESULTS...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.109.4.916

    authors: Hayakawa T,Terashima M,Kayukawa Y,Ohta T,Okada T

    更新日期:1996-04-01 00:00:00

  • Colonization of dental plaques: a reservoir of respiratory pathogens for hospital-acquired pneumonia in institutionalized elders.

    abstract:STUDY OBJECTIVES:Poor dental hygiene has been linked to respiratory pathogen colonization in residents of long-term care facilities. We sought to investigate the association between dental plaque (DP) colonization and lower respiratory tract infection in hospitalized institutionalized elders using molecular genotyping....

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.126.5.1575

    authors: El-Solh AA,Pietrantoni C,Bhat A,Okada M,Zambon J,Aquilina A,Berbary E

    更新日期:2004-11-01 00:00:00

  • Lung cancer chemoprevention: ACCP evidence-based clinical practice guidelines (2nd Edition).

    abstract:BACKGROUND:Lung cancer is the most common cause of cancer death in the United States. Cigarette smoking is the main risk factor. Former smokers are at a substantially increased risk for lung cancer compared with lifetime never-smokers. Chemoprevention is the use of specific agents to reverse, suppress, or prevent the p...

    journal_title:Chest

    pub_type: 杂志文章,实务指引

    doi:10.1378/chest.07-1348

    authors: Gray J,Mao JT,Szabo E,Kelley M,Kurie J,Bepler G,American College of Chest Physicians.

    更新日期:2007-09-01 00:00:00

  • Screening by specialists to reduce unnecessary test ordering in patients evaluated for tuberculosis.

    abstract:STUDY OBJECTIVE:To determine if screening by specialists could reduce unnecessary test ordering and reduce costs related to diagnostic workup in patients undergoing evaluation for tuberculosis. DESIGN:Prospective evaluation of expert opinion in consecutive patients suspected of having tuberculosis. SETTING:A large mu...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.114.3.681

    authors: Divinagracia RM,Harkin TJ,Bonk S,Schluger NW

    更新日期:1998-09-01 00:00:00

  • Measurement of respiratory sensation in interstitial lung disease. Evaluation of clinical dyspnea ratings and magnitude scaling.

    abstract::To evaluate measurements of respiratory sensation in ILD, we compared ratings of breathlessness from three clinical scales with the perceived magnitude of added elastic loads in 20 symptomatic ILD patients. Dyspnea ratings were obtained by two independent observers for each clinical method. Perceived magnitude of four...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.96.4.767

    authors: Mahler DA,Harver A,Rosiello R,Daubenspeck JA

    更新日期:1989-10-01 00:00:00

  • The treatment of pleuropulmonary amebiasis with metronidazole.

    abstract::One hundred forty patients with pleuropulmonary amebiasis were classified radiographically in order to rationalize therapy based on the use of metronidazole. Amebic testing with gel diffusion, serial chest x-ray films, and observation of the patient's response to treatment have dominated management of the patients, 13...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.73.5.647

    authors: Cameron EW

    更新日期:1978-05-01 00:00:00

  • African-American race and antibodies to topoisomerase I are associated with increased severity of scleroderma lung disease.

    abstract:STUDY OBJECTIVES:To determine whether African-American race is independently associated with lung disease in scleroderma. DESIGN:Retrospective review. SETTING:University medical center in Baltimore. PATIENTS:One hundred one patients with diffuse cutaneous scleroderma with available serum samples. MEASUREMENTS:Patie...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.114.3.801

    authors: Greidinger EL,Flaherty KT,White B,Rosen A,Wigley FM,Wise RA

    更新日期:1998-09-01 00:00:00

  • Interstitial Lung Abnormalities and the Clinical Course in Patients With COPD.

    abstract:BACKGROUND:The presence and progression of interstitial lung abnormalities (ILAs) is known to be associated with a decline of lung function and increased risk of mortality. RESEARCH QUESTION:We aimed to elucidate the clinical course according to ILAs in patients with COPD. STUDY DESIGN AND METHODS:A retrospective stu...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1016/j.chest.2020.08.017

    authors: Lee TS,Jin KN,Lee HW,Yoon SY,Park TY,Heo EY,Kim DK,Chung HS,Lee JK

    更新日期:2021-01-01 00:00:00

  • Effect of acute arteriolar vasodilation on capacitance and resistance in pulmonary arterial hypertension.

    abstract:BACKGROUND:Pulmonary vascular capacitance (PVC) is reduced in pulmonary arterial hypertension (PAH). In normal lung, PVC is largely a function of vascular compliance. In PAH, increased pulmonary vascular resistance (PVR) arises from the arterioles. PVR and PVC share pressure and volume variables. The dependency between...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.14-1461

    authors: Newman JH,Brittain EL,Robbins IM,Hemnes AR

    更新日期:2015-04-01 00:00:00

  • Torsades de pointes induced by erythromycin.

    abstract::We describe two patients who develop torsades de pointes in a temporal relationship to the intravenous administration of erythromycin lactobionate in the absence of other drugs or metabolic abnormalities known to cause the arrhythmia. We also review the current literature regarding this topic, including other case his...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.105.2.368

    authors: Gitler B,Berger LS,Buffa SD

    更新日期:1994-02-01 00:00:00

  • An optimal dose of perfluorocarbon for respiratory mechanics in partial liquid ventilation for dependent lung-dominant acute lung injury.

    abstract:BACKGROUND:Despite increasing knowledge about partial liquid ventilation (PLV), the optimal dose of perfluorocarbon (PFC) is not yet established. Because there exist normal regions in the lung with ARDS and because PLV in the normal lung results in worsened gas exchange, we postulated that the optimal dose of PFC for P...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.117.1.199

    authors: Lim CM,Koh Y,Jung BO,Lee SD,Kim WS,Kim DS,Kim WD

    更新日期:2000-01-01 00:00:00

  • Ventilatory effects of doxapram in conscious human subjects.

    abstract::The ventilatory effects of a bolus intravenous dose of doxapram (0.37 to 0.47 mg/kg) were studied in ten healthy normal subjects. An immediate significant (p less than 0.001) increase in minute ventilation (VE) was due, in equal part, to significant increases in tidal volume (VT) and frequency (f). The inspiratory (TI...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.85.5.600

    authors: Burki NK

    更新日期:1984-05-01 00:00:00

  • Validation of a novel risk score for severity of illness in acute exacerbations of COPD.

    abstract:BACKGROUND:Clinicians lack a validated tool for risk stratification in acute exacerbations of COPD (AECOPD). We sought to validate the BAP-65 (elevated BUN, altered mental status, pulse > 109 beats/min, age > 65 years) score for this purpose. METHODS:We analyzed 34,699 admissions to 177 US hospitals (2007) with either...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.10-3035

    authors: Shorr AF,Sun X,Johannes RS,Yaitanes A,Tabak YP

    更新日期:2011-11-01 00:00:00

  • Admission hyperglycemia and other risk factors as predictors of hospital mortality in a medical ICU population.

    abstract:BACKGROUND:Tight glycemic control is recommended for patients in the ICU, as hyperglycemia is associated with increased morbidity and mortality. DESIGN:Observational cohort of patients admitted to a 12-bed, inner-city, medical ICU (MICU). SUBJECTS:A total of 1,185 of 1,506 patients from July 1, 1999, to December 31, ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.128.5.3109

    authors: Freire AX,Bridges L,Umpierrez GE,Kuhl D,Kitabchi AE

    更新日期:2005-11-01 00:00:00

  • Cytologically proved malignant pleural effusions: distribution of transudates and exudates.

    abstract:PURPOSE:This study attempts to determine the distribution of transudates vs exudates in pathologically proved malignant pleural effusions and the necessity for cytologic studies in patients with a transudative effusion. MATERIALS AND METHODS:A retrospective review of all cytologically positive malignant pleural effusi...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.113.5.1302

    authors: Assi Z,Caruso JL,Herndon J,Patz EF Jr

    更新日期:1998-05-01 00:00:00

  • OSA and pulmonary hypertension: time for a new look.

    abstract::OSA is a common yet underdiagnosed disorder encountered in everyday practice. The disease is a unique physiologic stressor that contributes to the development or progression of many other disorders, particularly cardiovascular conditions. The pulmonary circulation is specifically affected by the intermittent hypoxic a...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.14-0614

    authors: Ismail K,Roberts K,Manning P,Manley C,Hill NS

    更新日期:2015-03-01 00:00:00

  • Changes in urinary uric acid excretion in obstructive sleep apnea before and after therapy with nasal continuous positive airway pressure.

    abstract:STUDY OBJECTIVE:To assess the utility of urinary uric acid excretion as a marker of nocturnal hypoxia in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS) before and after the institution of nasal continuous positive airway pressure (CPAP). DESIGN:Prospective, open. SETTING:Sleep Disorders Laboratory, V...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.113.6.1604

    authors: Sahebjani H

    更新日期:1998-06-01 00:00:00

  • Telescoping plugged catheter. An unusual way of diagnosing Pasteurella multocida pneumonia.

    abstract::We report a case of Pasteurella multocida pneumonia and its unusual clinical presentation. We also discuss the rarity of its diagnosis by TPC specimens and the delay in both an adequate diagnosis and treatment. ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.99.6.1517

    authors: Ruiz-Santana S,Antunez IA,Armas M,Rodriguez de Castro F,Manzano JL

    更新日期:1991-06-01 00:00:00

  • Decrease in beta-adrenergic receptors of lymphocytes in spontaneously occurring acute asthma.

    abstract::To clarify changes in beta-adrenergic receptor (BAR) density in spontaneously occurring acute asthma, BAR binding studies were performed on peripheral blood lymphocytes in eight asthmatic and ten normal subjects. Spirometry also was performed. Maximum binding capacity (Bmax) of BAR in acute asthma was significantly lo...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.104.2.508

    authors: Hataoka I,Okayama M,Sugi M,Inoue H,Takishima T,Shirato K

    更新日期:1993-08-01 00:00:00

  • Yield of bronchoscopy for the diagnosis of tuberculosis in patients with human immunodeficiency virus infection.

    abstract::The efficacy of bronchoscopy for the diagnosis of tuberculosis in patients infected with human immunodeficiency virus (HIV) has not been systematically evaluated. We therefore compared the diagnostic yield of bronchoscopy in 67 HIV-infected and 45 non-HIV-infected patients with culture-proven pulmonary tuberculosis. I...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.102.4.1040

    authors: Kennedy DJ,Lewis WP,Barnes PF

    更新日期:1992-10-01 00:00:00

  • Electrocardiographic manifestations and clinical significance of atrioventricular nodal alternating Wenckbach periods.

    abstract::Atrioventricular nodal alternating Wenckebach periods were defined as episodes of 2:1 atrioventricular block in which there was a gradual increase in transmission intervals of conducted beats ending in two or three consecutively blocked atrial impulses. This is one of the mechanisms whereby 2:1 atrioventricular block ...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.73.1.69

    authors: Castellanos A,Sung RJ,Aldrich JL,Maxwell M,Myerburg RJ

    更新日期:1978-01-01 00:00:00

  • Prevention of acute exacerbations of COPD: American College of Chest Physicians and Canadian Thoracic Society Guideline.

    abstract:BACKGROUND:COPD is a major cause of morbidity and mortality in the United States as well as throughout the rest of the world. An exacerbation of COPD (periodic escalations of symptoms of cough, dyspnea, and sputum production) is a major contributor to worsening lung function, impairment in quality of life, need for urg...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.14-1676

    authors: Criner GJ,Bourbeau J,Diekemper RL,Ouellette DR,Goodridge D,Hernandez P,Curren K,Balter MS,Bhutani M,Camp PG,Celli BR,Dechman G,Dransfield MT,Fiel SB,Foreman MG,Hanania NA,Ireland BK,Marchetti N,Marciniuk DD,Mularski

    更新日期:2015-04-01 00:00:00

  • Talc pleurodesis through indwelling pleural catheters for malignant pleural effusions: retrospective case series of a novel clinical pathway.

    abstract::Malignant pleural effusions cause significant morbidity, but there is no gold standard minimally invasive treatment. A new therapeutic approach combines talc pleurodesis and indwelling pleural catheters (IPCs) to enable outpatient management. This case series summarizes the safety and efficacy data of all patients (24...

    journal_title:Chest

    pub_type: 临床试验,杂志文章

    doi:10.1378/chest.14-0394

    authors: Ahmed L,Ip H,Rao D,Patel N,Noorzad F

    更新日期:2014-12-01 00:00:00

  • Esophagectomy after induction chemoradiation.

    abstract::The definition of a standard therapy for resectable esophageal cancer remains a clinical controversy. In the past decade, a variety of strategies have been developed in an attempt to improve local control and decrease the all too common problem of distant metastases. Preoperative treatment with radiotherapy or chemoth...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.116.suppl_3.466s

    authors: DeCamp MM Jr,Swanson SJ,Jaklitsch MT

    更新日期:1999-12-01 00:00:00

  • Automatic objective diagnosis of lymph nodal disease by B-mode images from convex-type echobronchoscopy.

    abstract:BACKGROUND:We investigated whether artificial neural networks (ANNs) could diagnose pathology of lymph nodes by feeding B-mode images from convex-type echobronchoscopy to ANNs. METHODS:Subjects comprised 91 patients who had undergone endobronchial ultrasonography transbronchial needle aspiration at our hospital betwee...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.07-1497

    authors: Tagaya R,Kurimoto N,Osada H,Kobayashi A

    更新日期:2008-01-01 00:00:00

  • Cyclophosphamide in the treatment of pulmonary diseases: survey of use, training, and practitioner knowledge base.

    abstract:OBJECTIVE:To assess pulmonologists' use, training in the use, and knowledge base of the drug cyclophosphamide. DESIGN:Survey through questionnaire. Testing of knowledge base before and after instructional conference. PARTICIPANTS AND METHODS:Pulmonologists (94 attendings, 31 fellows), selected randomly at the 1996 an...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.116.5.1159

    authors: Cozzi PJ,Hall JB

    更新日期:1999-11-01 00:00:00

  • A 26-Year-Old Woman With a Small Right Lung and a Right-Sided Heart.

    abstract::A 26-year-old woman presented with abnormal findings on a chest radiograph. She had no significant history other than a fever 4 months prior to presentation that had resolved without a definite cause identified. She denied cough, shortness of breath, chest pain, history of smoking, environmental exposures, or prior pr...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1016/j.chest.2015.08.023

    authors: Albores J,Smith MI

    更新日期:2016-03-01 00:00:00

  • Genetics and pulmonary hypertension.

    abstract::Primary pulmonary hypertension (PPH) is a serious pulmonary vascular disease occurring mostly in adult women. Although its occurrence in families was reported within a few years after the original clinical report, PPH was formerly believed rarely to have a genetic basis. Recent progress has not only clarified a basic ...

    journal_title:Chest

    pub_type: 杂志文章,评审

    doi:10.1378/chest.122.6_suppl.284s

    authors: Loyd JE

    更新日期:2002-12-01 00:00:00

  • Prognosis and recurrent patterns in bronchioloalveolar carcinoma.

    abstract:STUDY OBJECTIVE:Bronchioloalveolar carcinoma (BAC) is an uncommon pulmonary neoplasm with various radiologic and clinical presentations. In this article, we analyze the initial radiologic findings, TNM stagings, surgical types, and radiologic features of recurrence, and correlate them with patient survival. DESIGN:A r...

    journal_title:Chest

    pub_type: 杂志文章

    doi:10.1378/chest.118.4.940

    authors: Liu YY,Chen YM,Huang MH,Perng RP

    更新日期:2000-10-01 00:00:00