Effect of enzyme inducers on metabolism of 1-nitropyrene in human hepatoma cell line HepG2.

Abstract:

:We measured the response of HepG2 cells to the classic cytochrome (cyt.) P-450 inducers 3-methylcholanthrene (3-MC) and phenobarbital (PB), by evaluating oxidative and/or reductive metabolism of the nitroarenes, 1-NP and 1,6-dinitropyrene (1,6-DNP), in control and induced cells. In HepG2 cells, 3-MC induces ring-hydroxylation of 1-NP, whereas PB stimulates its nitroreduction. PB induces NADPH-cyt. c reductase, but does not affect other cytosolic and microsomal enzymes which contribute to 1-NP nitroreduction in these cells. However, PB-inducible nitroreductase activity seems to be associated primarily with cyt. P-450 isoenzymatic form(s), as indicated by the requirement for NADPH and the response to specific inhibitors such as alpha-naphthoflavone and CO.

journal_name

Chem Biol Interact

authors

Belisario MA,Arena AR,Pecce R,Borgia R,Staiano N,De Lorenzo F

doi

10.1016/0009-2797(91)90057-e

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

253-68

issue

3

eissn

0009-2797

issn

1872-7786

pii

0009-2797(91)90057-E

journal_volume

78

pub_type

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