Abstract:
:The combined effects of ethanol and hypoxia on the conversion of xanthine dehydrogenase (D form) to xanthine oxidase (O form) and on the leakage of the enzyme from isolated rat hepatocytes was studied. Time-dependent death of cells occurred during incubation in hypoxic conditions. Ethanol (40 mM) had only a moderate effect on viability in aerobiosis, but accelerated the loss of hypoxic cells, which was 96% after 3 h of incubation. In hypoxic conditions, the xanthine oxidase was gradually converted from D into O form. The conversion was complete in 3 h, and was accelerated by 1 mM xanthine or by ethanol, in a concentration-related manner. Hypoxia brought about a progressive leakage of xanthine oxidase from hepatocytes, which was accelerated by ethanol in a concentration-dependent manner. The enzyme found outside hepatocytes was mostly in its O form. The xanthine oxidase of hepatocytes cytosol was converted from D into O form by human plasma or serum. In all cases the conversion could be completely reverted by treatment of the extract with dithiothreitol.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Battelli MG,Abbondanza A,Stirpe Fdoi
10.1016/0009-2797(92)90093-zsubject
Has Abstractpub_date
1992-06-15 00:00:00pages
73-84issue
1eissn
0009-2797issn
1872-7786pii
0009-2797(92)90093-Zjournal_volume
83pub_type
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