Effects of hypoxia and ethanol on xanthine oxidase of isolated rat hepatocytes: conversion from D to O form and leakage from cells.


:The combined effects of ethanol and hypoxia on the conversion of xanthine dehydrogenase (D form) to xanthine oxidase (O form) and on the leakage of the enzyme from isolated rat hepatocytes was studied. Time-dependent death of cells occurred during incubation in hypoxic conditions. Ethanol (40 mM) had only a moderate effect on viability in aerobiosis, but accelerated the loss of hypoxic cells, which was 96% after 3 h of incubation. In hypoxic conditions, the xanthine oxidase was gradually converted from D into O form. The conversion was complete in 3 h, and was accelerated by 1 mM xanthine or by ethanol, in a concentration-related manner. Hypoxia brought about a progressive leakage of xanthine oxidase from hepatocytes, which was accelerated by ethanol in a concentration-dependent manner. The enzyme found outside hepatocytes was mostly in its O form. The xanthine oxidase of hepatocytes cytosol was converted from D into O form by human plasma or serum. In all cases the conversion could be completely reverted by treatment of the extract with dithiothreitol.


Chem Biol Interact


Battelli MG,Abbondanza A,Stirpe F




Has Abstract


1992-06-15 00:00:00














  • Oroxylin A prevents alcohol-induced hepatic steatosis through inhibition of hypoxia inducible factor 1alpha.

    abstract::Accumulating data reveal that oroxylin A has beneficial effects against chronic liver disease. The previously studies showed oroxylin A, a flavonoid extracted from Scutellariae radix, improved acute liver injury and accelerated liver regeneration in vivo. However, it's unclear that the effect of oroxylin A on alcoholi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Jin H,Lian N,Bian M,Zhang C,Chen X,Shao J,Wu L,Chen A,Guo Q,Zhang F,Zheng S

    更新日期:2018-04-01 00:00:00

  • Efficacy of novel phenoxyalkyl pyridinium oximes as brain-penetrating reactivators of cholinesterase inhibited by surrogates of sarin and VX.

    abstract::Pyridinium oximes are strong nucleophiles and many are effective reactivators of organophosphate-inhibited cholinesterase (ChE). However, the current oxime reactivators are ineffective at crossing the blood-brain barrier and reactivating brain ChE in the intact organism. Our laboratories have developed a series of sub...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Chambers JE,Chambers HW,Funck KE,Meek EC,Pringle RB,Ross MK

    更新日期:2016-11-25 00:00:00

  • Inactivation of GAP-43 due to the depletion of cellular calcium by the Pb and amyloid peptide induced toxicity: An in vitro approach.

    abstract::Environmental pollutant, Lead (Pb) is known to induce neurotoxicity in human. The central nervous system is the most vulnerable to the minute levels of Pb induced toxicity. Pb has been linked to Alzheimer's disease (AD) as a probable risk factor, as it shows epigenetic and developmental link associated with Alzheimer'...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Ayyalasomayajula N,Bandaru M,Dixit PK,Ajumeera R,Chetty CS,Challa S

    更新日期:2020-01-25 00:00:00

  • Expression of AKR1B1, AKR1C3 and other genes of prostaglandin F2α biosynthesis and action in ovarian endometriosis tissue and in model cell lines.

    abstract::Endometriosis is a frequent benign gynecological disease characterized by endometrial tissue outside the uterine cavity. The estimated prevalence in the general population is 6-10%, but this reaches 30-50% in women with infertility and/or pain. As ectopic tissue within the pelvic cavity provokes inflammation, endometr...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Sinreih M,Anko M,Kene NH,Kocbek V,Rižner TL

    更新日期:2015-06-05 00:00:00

  • The involvement of ionic interactions during asbestos-induced enzyme release from polymorphonuclear leukocytes.

    abstract::Chrysotile asbestos permeabilizes the plasma membrane of rabbit polymorphonuclear leukocytes (PMNs) which is evident from the release of the cytoplasmic enzyme lactate dehydrogenase (LDH) from the cell. When Ca2+ is present in the medium exocytosis is observed, evident from the release of the granule associated enzyme...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Elferink JG,Deierkauf M,Kramps JA,Koerten HK

    更新日期:1989-01-01 00:00:00

  • A review of mechanisms underlying anticarcinogenicity by brassica vegetables.

    abstract::The mechanisms by which brassica vegetables might decrease the risk of cancer are reviewed in this paper. Brassicas, including all types of cabbages, broccoli, cauliflower and Brussels sprouts, may be protective against cancer due to their relatively high glucosinolate content. Glucosinolates are usually broken down t...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Verhoeven DT,Verhagen H,Goldbohm RA,van den Brandt PA,van Poppel G

    更新日期:1997-02-28 00:00:00

  • Microsomal metabolism of hexavalent chromium. Inhibitory effect of oxygen and involvement of cytochrome P-450.

    abstract::The reduction of hexavalent chromium (Cr(VI] by rat liver microsomes was studied. With 15-120 microM Na2CrO4 microsomes (0.5 mg protein/ml) effectively reduced Cr(VI) in the presence of NADPH provided anaerobic conditions. Phenobarbital (PB) and Aroclor 1254 (PCB) pretreatment increased microsomal Cr(VI) reduction whi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Mikalsen A,Alexander J,Ryberg D

    更新日期:1989-01-01 00:00:00

  • Selective protection by stably transfected human ALDH3A1 (but not human ALDH1A1) against toxicity of aliphatic aldehydes in V79 cells.

    abstract::Toxic medium chain length alkanals, alkenals, and 4-hydroxyalkenals that are generated during lipid peroxidation are potential substrates for aldehyde dehydrogenase (ALDH) isoforms. We have developed transgenic cell lines to examine the potential for either human ALDH1A1 or ALDH3A1 to protect against damage mediated b...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Townsend AJ,Leone-Kabler S,Haynes RL,Wu Y,Szweda L,Bunting KD

    更新日期:2001-01-30 00:00:00

  • Caffeine does not bind covalently to liver microsomes from different animal species and to proteins and DNA from perfused rat liver.

    abstract::Caffeine was found not to bind covalently to liver microsomal proteins from mice, rats and rabbits. Microsomes metabolized caffeine only to a limited extent, the highest rate (about 2% of the substrate concentration) being obtained with rabbit microsomal preparations. The rat liver perfusion technique represents a goo...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Szczawinska K,Ginelli E,Bartosek I,Gambazza C,Pantarotto C

    更新日期:1981-03-15 00:00:00

  • Effects of organosulfurs, isothiocyanates and vitamin C towards hydrogen peroxide-induced oxidative DNA damage (strand breaks and oxidized purines/pyrimidines) in human hepatoma cells.

    abstract::The aim of this study was to evaluate the effects of organosulfurs, isothiocyanates and vitamin C towards hydrogen peroxide-induced DNA damage (DNA strand breaks and oxidized purines/pyrimidines) in human hepatoma cells (HepG2), using the Comet assay. Treatment with hydrogen peroxide (H(2)O(2)) increased the levels of...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Arranz N,Haza AI,García A,Delgado E,Rafter J,Morales P

    更新日期:2007-08-15 00:00:00

  • Correlation between the substrate structure and the rate of acetylcholinesterase hydrolysis modeled with the combined quantum mechanical/molecular mechanical studies.

    abstract::The combined quantum mechanical-molecular mechanical (QM/MM) based computational scheme for modeling the structure-reaction rate correlations was elaborated for the hydrolysis of the set of neutral esters in the active site of acetylcholinesterase (AChE). The energy barriers of hydrolysis were estimated on the basis o...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Lushchekina SV,Nemukhin AV,Morozov DI,Varfolomeev SD

    更新日期:2010-09-06 00:00:00

  • Uncoupling effect of fungal hydroxyanthraquinones on mitochondrial oxidative phosphorylation.

    abstract::Structure-uncoupling activity relationship of seven anthraquinone derivatives were investigated using rat liver mitochondria. Three compounds bearing the free hydroxyl group at the beta-position of their anthraquinone nucleus (1,3,6,8-tetrahydroxyanthraquinone, 1-acetyl-2,4,5,7-tetrahydroxy-9,10-anthracenedione and sk...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Betina V,Kuzela S

    更新日期:1987-01-01 00:00:00

  • Bone abnormalities in young male rats with iron intervention and possible mechanisms.

    abstract::Studies suggest iron overload may cause bone lesion. The mechanisms are not well understood at present. Therefore, this study was designed to observe the effect of iron overload on bone metabolism in young male rats and explore its possible mechanism. Eighteen SD rats were randomly assigned to iron-loading and control...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Li Y,Bai B,Zhang Y

    更新日期:2018-01-05 00:00:00

  • Identification of amino acid residues involved in substrate recognition of L-xylulose reductase by site-directed mutagenesis.

    abstract::L-Xylulose reductase (XR) catalyzes the oxidoreduction between xylitol and L-xylulose in the uronate cycle. The enzyme has been shown to be identical to diacetyl reductase, an enzyme that reduces alpha-dicarbonyl compounds. XR belongs to the short-chain dehydrogenase/reductase family, and shows high sequence identity ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Ishikura S,Isaji T,Usami N,Nakagawa J,El-Kabbani O,Hara A

    更新日期:2003-02-01 00:00:00

  • Anti-inflammatory and anti-gouty-arthritic effect of free Ginsenoside Rb1 and nano Ginsenoside Rb1 against MSU induced gouty arthritis in experimental animals.

    abstract::Ginsenoside Rb1 (GsRb1) is the best constituent of ginseng and although it shows clinical efficacy as an antineoplastic, antioxidative and antirheumatic agent, its oral bioavailability is poor due to its limited solubility. In this study, the solubility of GsRb1 was improved by encapsulating it in polymeric nanocapsul...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Liu Y,Zhu H,Zhou W,Ye Q

    更新日期:2020-12-01 00:00:00

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin suppress AChE activity in NGF treated PC12 cells.

    abstract::PC12 is a well studied cell model for neuronal differentiation. AChE is also considered as a marker for neuronal differentiation. In this study, we detected the change of AChE activity during the NGF induced differentiation of PC 12 cells, and targeted on the ratio of the activity of AChE on the cell surface, and foun...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Xu L,Chen Y,Xie HQH,Xu T,Fu H,Zhang S,Tsim KWK,Bi CWC,Zhao B

    更新日期:2016-11-25 00:00:00

  • Modulatory role of lipoic acid on adriamycin-induced testicular injury.

    abstract::The present study investigated the protective efficacy of dl-alpha-lipoic acid (LA) on adriamycin (ADR)-induced oxidative damage in rat testis. Adult male albino rats of Wistar strain were administered ADR (1 mg/kg body weight, i.v.), once a week for 10 weeks. ADR injected rats showed increased oxidative stress with a...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Prahalathan C,Selvakumar E,Varalakshmi P

    更新日期:2006-03-25 00:00:00

  • In vivo efficacy of the Reactive Skin Decontamination Lotion (RSDL®) kit against organophosphate and carbamate pesticides.

    abstract::In this study, we assessed the efficacy of the Reactive Skin Decontamination Lotion (RSDL®) Kit against parathion and aldicarb pesticide dermal exposure in a guinea pig model. The pesticides inhibit acetylcholinesterase (AChE) leading to signs and symptoms of hyperactivity of organs due to accumulation of acetylcholin...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Fentabil M,Gebremedhin M,Barry J,Mikler J,Cochrane L

    更新日期:2020-02-25 00:00:00

  • Characterization of the purified microsomal FAD-containing monooxygenase from mouse and pig liver.

    abstract::The FAD-containing monooxygenase (FMO) has been purified from both mouse and pig liver microsomes by similar purification procedures. Characterization of the enzyme from these two sources has revealed significant differences in catalytic and immunological properties. The pH optimum of mouse FMO is slightly higher than...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Sabourin PJ,Hodgson E

    更新日期:1984-09-15 00:00:00

  • Protective effect of rat aldo-keto reductase (AKR1C15) on endothelial cell damage elicited by 4-hydroxy-2-nonenal.

    abstract::4-Hydroxy-2-nonenal (HNE), a major reactive product of lipid peroxidation, is believed to play a central role in atherogenic actions triggered by oxidized lipoproteins. An aldo-keto reductase (AKR) 1C15 efficiently reduces HNE and is distributed in many rat tissues including endothelial cells. In this study, we invest...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Matsunaga T,Shinoda Y,Inoue Y,Endo S,El-Kabbani O,Hara A

    更新日期:2011-05-30 00:00:00

  • Screening of O-ethyl O-4-nitrophenyl phosphoramidate (ENPP) for delayed neuropathic potential.

    abstract::O-Ethyl-O-4-nitrophenylphosphoramidate is a short-acting anticholinesterase and a possible candidate for a prophylactic agent against nerve agents since human acetylcholinesterase inhibited by this agent undergoes rapid spontaneous reactivation which can be accelerated further, if necessary, by treatment with oximes. ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Johnson MK,Read DJ

    更新日期:1993-06-01 00:00:00

  • Induction of rat liver O6-alkylguanine-DNA alkyltransferase by bleomycin.

    abstract::Alkyl adducts at the O6-position of guanine constitute promutagenic DNA lesions likely to be involved in the initiation of malignant transformation. They can be removed by a cellular acceptor protein termed O6-alkylguanine-DNA alkyltransferase (AT). In rat liver this repair enzyme can be induced by a variety of hepato...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Schmerold I,Spath A

    更新日期:1986-12-01 00:00:00

  • Dichlorophenyl phosphoramidates as substrates for avian and mammalian liver phosphotriesterases: activity levels, calcium dependence and stereospecificity.

    abstract::The present study shows the existence of both Ca2+-dependent and EDTA-resistant hydrolysing activities against HDCP and paraoxon in the particulate and soluble fractions of hen, rat and rabbit liver. HDCP was more extensively hydrolysed than paraoxon in both subcellular fractions and each of three individuals of the t...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Monroy-Noyola A,Sogorb MA,Díaz-Alejo N,Níguez N,Barril J,Vicedo JL,Escudero MA,Vilanova E

    更新日期:1999-05-14 00:00:00

  • Gastroprotective activity of alkaloid extract and 2-phenylquinoline obtained from the bark of Galipea longiflora Krause (Rutaceae).

    abstract::As part of our continuing search for bioactive natural products from plants, the present study was carried out in order to evaluate the gastroprotective properties of alkaloid extract and 2-phenylquinoline obtained from the bark of Galipea longiflora (Rutaceae). Anti-ulcer assays were performed using the following pro...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Zanatta F,Gandolfi RB,Lemos M,Ticona JC,Gimenez A,Clasen BK,Cechinel Filho V,de Andrade SF

    更新日期:2009-07-15 00:00:00

  • Radiomimetic property of furazolidone and the caffeine enhancement of its lethal action on the vibrios.

    abstract::Sensitivities of the strains belonging to four vibrio biotypes to the action of furazolidone were investigated. Vibrio cholerae (classical) was most and Vibrio parahaemolyticus least sensitive to this drug. Statistical analyses revealed significant differences between any two of the four types of vibrio in respect of ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Banerjee SK,Chatterjee SN

    更新日期:1981-11-01 00:00:00

  • Multiple mechanisms of cell death induced by chelidonine in MCF-7 breast cancer cell line.

    abstract::In a preliminary study screening anti-proliferative natural alkaloids, a very potent benzophenanthridine, chelidonine showed strong cytotoxicity in cancer cells. While several modes of death have been identified, most of anti-cancer attempts have focused on stimulation of cells to undergo apoptosis. Chelidonine seems ...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Noureini SK,Esmaili H

    更新日期:2014-11-05 00:00:00

  • Studies on the mechanism of reduction of azo dye carcinogens by rat liver microsomal cytochrome P-450.

    abstract::This laboratory has described the azoreduction of p-dimethylaminoazobenzene (1c) by rat liver microsomal cytochrome P-450. To elucidate the mechanisms involved, the reduction of structurally related azobenzenes by hepatic microsomes was investigated. High substrate reactivity was observed for 1c, its corresponding sec...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Zbaida S,Stoddart AM,Levine WG

    更新日期:1989-01-01 00:00:00

  • Influence of quinidine on the intestinal secretion of digoxin and digitoxin in guinea pigs.

    abstract::The secretion of digoxin and digitoxin into in situ perfused jejunal and colonic segments of normal or quinidine treated guinea pigs was studied. Quinidine was administered intravenously by constant rate infusion resulting in a quinidine plasma concentration of about 6 micrograms/ml. After 2 h digoxin or digitoxin was...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Schäfer SG,Schuhmann G,Doering W,Fichtl B

    更新日期:1985-10-01 00:00:00

  • Inhibition of CYP1A1 enzyme activity in mouse hepatoma cell culture by soybean isoflavones.

    abstract::The mechanisms by which soybean- and soybean isoflavone-enriched diets inhibit carcinogenesis are not known. We found that the isoflavones genistin and daidzin, and their respective aglucone forms daidzein and genistein, block 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; dioxin)-induced CYP1A1 enzyme activity. This inhi...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章


    authors: Shertzer HG,Puga A,Chang C,Smith P,Nebert DW,Setchell KD,Dalton TP

    更新日期:1999-11-15 00:00:00

  • Hypotensive effects of genistein: From chemistry to medicine.

    abstract::Genistein (4', 5, 7-trihydroxyisoflavone), a naturally occurring flavonoid characteristic of Leguminoseae plants, is a phyto-oestrogen exerting oestrogenic activity as both an agonist and an antagonist substance. A large body of evidence suggests that genistein possesses many physiological and pharmacological properti...

    journal_title:Chemico-biological interactions

    pub_type: 杂志文章,评审


    authors: Sureda A,Sanches Silva A,Sánchez-Machado DI,López-Cervantes J,Daglia M,Nabavi SF,Nabavi SM

    更新日期:2017-04-25 00:00:00