Copper transfer from Rhus vernicifera laccase.


:Tree laccase, a multi-copper oxidase, has been studied as a copper donor in conjunction with the demetalated forms of three blue copper proteins. Copper transfer could be observed under reducing conditions in the absence of air. Only about 10% of the total copper in laccase could be transferred regardless of the amount of acceptor present in solution, hence, the laccase is heterogeneous as isolated. Potential sources of the heterogeneity are considered. After transfer, laccase could be partially resolved into copper-deficient and nearly holoprotein fractions that would not donate copper when recombined with acceptor protein. EPR results in conjunction with thiol titrations indicate that there is no net loss of type 1 copper from laccase but that there is loss of type 2 copper as well as a small amount of type 3 copper. Very little transfer is observed when type 2-depleted laccase is used as the donor. Finally, the implications that these results could have in the elucidation of possibly more physiologically relevant processes are briefly summarized.


J Inorg Biochem


Meadows KA,Morie-Bebel MM,McMillin DR




Has Abstract


1991-03-01 00:00:00














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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Ianelli S,Mazza P,Orcesi M,Pelizzi C,Pelizzi G,Zani F

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  • The systematic study of aluminium speciation in medium concentrated aqueous solutions.

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    journal_title:Journal of inorganic biochemistry

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    pub_type: 杂志文章,评审


    authors: Santolini J

    更新日期:2011-02-01 00:00:00

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    authors: Magrì A,D'Alessandro F,Distefano DA,Campagna T,Pappalardo G,Impellizzeri G,La Mendola D

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    pub_type: 杂志文章


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    更新日期:2009-04-01 00:00:00

  • Ligand replacement study at the His120 site of purple CuA azurin.

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Berry SM,Wang X,Lu Y

    更新日期:2000-01-15 00:00:00

  • Spectroscopic investigation of isonitrile complexes of ferric and ferrous microperoxidase 8.

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    pub_type: 杂志文章


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  • Micropattern immobilization of polysaccharide.

    abstract::Two types of polysaccharides, sulfated hyaluronic acid and heparin, were pattern-immobilized on a poly(ethylene terephthalate) and polystyrene film, respectively, in a specific pattern by photolithography. Sulfated hyaluronic acid was prepared from hylaronic acid by the treatment of sulfur trioxide/pyridine complex. H...

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    pub_type: 杂志文章


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  • The presence of a SO molecule in [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki as detected by mass spectrometry.

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    pub_type: 杂志文章


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  • Reduction of DL-selenocystine and isolation of L-seleoncysteine.

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    pub_type: 杂志文章,评审


    authors: Vasák M,Hasler DW,Faller P

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Rubino S,Portanova P,Albanese A,Calvaruso G,Orecchio S,Fontana G,Stocco GC

    更新日期:2007-10-01 00:00:00

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Matsuo T,Ito K,Nakashima Y,Hisaeda Y,Hayashi T

    更新日期:2008-02-01 00:00:00

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    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章


    authors: Jastrzab R,Runka T,Skowronek P,Lomozik L

    更新日期:2010-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Kiersztan A,Lukasinska I,Baranska A,Lebiedzinska M,Nagalski A,Derlacz RA,Bryla J

    更新日期:2007-03-01 00:00:00