Abstract:
:Recombination enhancer (RE) is essential for regulating donor preference during yeast mating type switching. In this study, by using minichromosome affinity purification (MAP) and mass spectrometry, we found that yeast Ku80p is associated with RE in MATa cells. Chromatin immunoprecipitation assays confirmed its occupancy in vivo. Deletion of YKU80 results in altered chromatin structure in the RE region and more importantly causes a dramatic decrease of HML usage in MATa cells. We also detect directional movement of yKu80p from the RE towards HML during switching. These results indicate a novel function of yeast Ku80p in regulating mating type switching.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ruan C,Workman JL,Simpson RTdoi
10.1128/MCB.25.19.8476-8485.2005subject
Has Abstractpub_date
2005-10-01 00:00:00pages
8476-85issue
19eissn
0270-7306issn
1098-5549pii
25/19/8476journal_volume
25pub_type
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