Retroviral vector system for the study of cDNA gene formation.

Abstract:

:A retroviral vector system was developed to study the retrotransposition of RNAs lacking all cis-acting sequences required for normal retroviral replication. Our experiments indicate that such RNAs can be encapsidated in retroviral proteins, reverse transcribed, and integrated to form functional cDNA genes in infected cells. The frequency of this process, however, was approximately 8 orders of magnitude less than that of normal retroviral replication. The efficiency was limited at each step in this process. Investigation of seven cDNA genes by Southern blot analysis revealed that all of them were truncated at either the 3' or the 5' end or both. These truncations are not seen with natural cDNA genes and raise the question of retroviral involvement in their formation.

journal_name

Mol Cell Biol

authors

Dornburg R,Temin HM

doi

10.1128/mcb.8.6.2328

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

2328-34

issue

6

eissn

0270-7306

issn

1098-5549

journal_volume

8

pub_type

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