Retroviral vector system for the study of cDNA gene formation.


:A retroviral vector system was developed to study the retrotransposition of RNAs lacking all cis-acting sequences required for normal retroviral replication. Our experiments indicate that such RNAs can be encapsidated in retroviral proteins, reverse transcribed, and integrated to form functional cDNA genes in infected cells. The frequency of this process, however, was approximately 8 orders of magnitude less than that of normal retroviral replication. The efficiency was limited at each step in this process. Investigation of seven cDNA genes by Southern blot analysis revealed that all of them were truncated at either the 3' or the 5' end or both. These truncations are not seen with natural cDNA genes and raise the question of retroviral involvement in their formation.


Mol Cell Biol


Dornburg R,Temin HM




Has Abstract


1988-06-01 00:00:00












  • Termination region in rRNA genes from a eucaryotic thermophile, Thermomyces lanuginosus.

    abstract::S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Walker K,Wong WM,Nazar RN

    更新日期:1990-01-01 00:00:00

  • Functional analysis of the yeast Glc7-binding protein Reg1 identifies a protein phosphatase type 1-binding motif as essential for repression of ADH2 expression.

    abstract::In Saccharomyces cerevisiae, the protein phosphatase type 1 (PP1)-binding protein Reg1 is required to maintain complete repression of ADH2 expression during growth on glucose. Surprisingly, however, mutant forms of the yeast PP1 homologue Glc7, which are unable to repress expression of another glucose-regulated gene, ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Dombek KM,Voronkova V,Raney A,Young ET

    更新日期:1999-09-01 00:00:00

  • Phosphorylation of Par-4 by protein kinase A is critical for apoptosis.

    abstract::Despite distinct dissimilarities, diverse cancers express several common protumorigenic traits. We present here evidence that the proapoptotic protein Par-4 utilizes one such common tumorigenic trait to become selectively activated and induce apoptosis in cancer cells. Elevated protein kinase A (PKA) activity noted in...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gurumurthy S,Goswami A,Vasudevan KM,Rangnekar VM

    更新日期:2005-02-01 00:00:00

  • Posttranscriptional regulation of rat growth hormone gene expression: increased message stability and nuclear polyadenylation accompany thyroid hormone depletion.

    abstract::In thyroid hormone-depleted rats, the rate of transcription of the growth hormone (GH) gene in the anterior pituitary gland is lower than the rate in euthyroid controls, and there is a corresponding reduction in the abundance of the GH mRNA. Concomitantly, the poly(A) tail of the GH mRNA increases in length. Examinati...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Murphy D,Pardy K,Seah V,Carter D

    更新日期:1992-06-01 00:00:00

  • Essential role of insulin receptor substrate 1 (IRS-1) and IRS-2 in adipocyte differentiation.

    abstract::To investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, the two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1(-/-)), IRS-2 deficient (IRS-2(-/-)), and IRS-1 IRS-2 dou...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Miki H,Yamauchi T,Suzuki R,Komeda K,Tsuchida A,Kubota N,Terauchi Y,Kamon J,Kaburagi Y,Matsui J,Akanuma Y,Nagai R,Kimura S,Tobe K,Kadowaki T

    更新日期:2001-04-01 00:00:00

  • Transformation by v-myb correlates with trans-activation of gene expression.

    abstract::The v-myb oncogene of avian myeloblastosis virus causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its protein product p48v-myb is a nuclear, sequence-specific, DNA-binding protein which activates gene expression in transient DNA transfection studies. To investigate the rela...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Lane T,Ibanez C,Garcia A,Graf T,Lipsick J

    更新日期:1990-06-01 00:00:00

  • Stability of a human SWI-SNF remodeled nucleosomal array.

    abstract::SWI-SNF alters DNA-histone interactions within a nucleosome in an ATP-dependent manner. These alterations cause changes in the topology of a closed circular nucleosomal array that persist after removal of ATP from the reaction. We demonstrate here that a remodeled closed circular array will revert toward its original ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Guyon JR,Narlikar GJ,Sullivan EK,Kingston RE

    更新日期:2001-02-01 00:00:00

  • Analysis of primary structural determinants that distinguish the centromere-specific function of histone variant Cse4p from histone H3.

    abstract::Cse4p is a variant of histone H3 that has an essential role in chromosome segregation and centromere chromatin structure in budding yeast. Cse4p has a unique 135-amino-acid N terminus and a C-terminal histone-fold domain that is more than 60% identical to histone H3 and the mammalian centromere protein CENP-A. Cse4p a...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Keith KC,Baker RE,Chen Y,Harris K,Stoler S,Fitzgerald-Hayes M

    更新日期:1999-09-01 00:00:00

  • The transcriptional coactivator SAYP is a trithorax group signature subunit of the PBAP chromatin remodeling complex.

    abstract::SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control. BAP and PBAP are the fly representatives of the two evolutionarily conserved major subclasses of SWI/SNF remodelers. Both complexes share seven core subunits, including the Brahma ATPase,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Chalkley GE,Moshkin YM,Langenberg K,Bezstarosti K,Blastyak A,Gyurkovics H,Demmers JA,Verrijzer CP

    更新日期:2008-05-01 00:00:00

  • Rad53 checkpoint kinase phosphorylation site preference identified in the Swi6 protein of Saccharomyces cerevisiae.

    abstract::Rad53 of Saccharomyces cerevisiae is a checkpoint kinase whose structure and function are conserved among eukaryotes. When a cell detects damaged DNA, Rad53 activity is dramatically increased, which ultimately leads to changes in DNA replication, repair, and cell division. Despite its central role in checkpoint signal...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sidorova JM,Breeden LL

    更新日期:2003-05-01 00:00:00

  • Redistribution of activated pp60c-src to integrin-dependent cytoskeletal complexes in thrombin-stimulated platelets.

    abstract::Thrombin stimulation of platelets induces a transient increase in the specific activity of pp60c-src followed by a redistribution of pp60c-src to the Triton X-100-insoluble, cytoskeleton-rich fraction. Concomitant with the observed increase in pp60c-src activity was a rapid dephosphorylation of tyrosine 527 in 10 to 1...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Clark EA,Brugge JS

    更新日期:1993-03-01 00:00:00

  • Deficiency of reproductive tract alpha(1,2)fucosylated glycans and normal fertility in mice with targeted deletions of the FUT1 or FUT2 alpha(1,2)fucosyltransferase locus.

    abstract::The fucose alpha(1-->2) galactose beta structure is expressed by uterine epithelial cells in the mouse and has been implicated in blastocyst adhesion events thought to be required for murine implantation. Fucalpha(1-->2)Galbeta moieties and cognate fucosyltransferases are also expressed by epithelial cells of the male...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Domino SE,Zhang L,Gillespie PJ,Saunders TL,Lowe JB

    更新日期:2001-12-01 00:00:00

  • A conserved alpha-helical motif mediates the interaction of Sp1-like transcriptional repressors with the corepressor mSin3A.

    abstract::Sp1-like proteins are defined by three highly homologous C(2)H(2) zinc finger motifs that bind GC-rich sequences found in the promoters of a large number of genes essential for mammalian cell homeostasis. Here we report that TIEG2, a transforming growth factor beta-inducible Sp1-like protein with antiproliferative fun...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhang JS,Moncrieffe MC,Kaczynski J,Ellenrieder V,Prendergast FG,Urrutia R

    更新日期:2001-08-01 00:00:00

  • Inducible transcriptional activation of the human immunodeficiency virus long terminal repeat by protein kinase inhibitors.

    abstract::The protein kinase inhibitor 2-aminopurine (2-AP) greatly stimulated expression in human promonocytes-macrophages of plasmid constructs carrying various reporter genes (chloramphenicol acetyltransferase, lacZ, firefly luciferase [luc], and Salmonella typhimurium histidinol dehydrogenase [his]) driven by the human immu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Brown FL,Tahaoglu E,Graham GJ,Maio JJ

    更新日期:1993-09-01 00:00:00

  • Identification and characterization of SAP25, a novel component of the mSin3 corepressor complex.

    abstract::The transcriptional corepressor mSin3 is associated with histone deacetylases (HDACs) and is utilized by many DNA-binding transcriptional repressors. We have cloned and characterized a novel mSin3A-binding protein, SAP25. SAP25 binds to the PAH1 domain of mSin3A, associates with the mSin3A-HDAC complex in vivo, and re...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Shiio Y,Rose DW,Aur R,Donohoe S,Aebersold R,Eisenman RN

    更新日期:2006-02-01 00:00:00

  • Wnt proteins induce dishevelled phosphorylation via an LRP5/6- independent mechanism, irrespective of their ability to stabilize beta-catenin.

    abstract::Wnt glycoproteins play essential roles in the development of metazoan organisms. Many Wnt proteins, such as Wnt1, activate the well-conserved canonical Wnt signaling pathway, which results in accumulation of beta-catenin in the cytosol and nucleus. Other Wnts, such as Wnt5a, activate signaling mechanisms which do not ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: González-Sancho JM,Brennan KR,Castelo-Soccio LA,Brown AM

    更新日期:2004-06-01 00:00:00

  • CtBP and associated LSD1 are required for transcriptional activation by NeuroD1 in gastrointestinal endocrine cells.

    abstract::Gene expression programs required for differentiation depend on both DNA-bound transcription factors and surrounding histone modifications. Expression of the basic helix-loop-helix (bHLH) protein NeuroD1 is restricted to endocrine cells in the gastrointestinal (GI) tract, where it is important for endocrine differenti...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ray SK,Li HJ,Metzger E,Schüle R,Leiter AB

    更新日期:2014-06-01 00:00:00

  • DNA-mediated transfer of a human gene required for low-density lipoprotein receptor expression and for multiple Golgi processing pathways.

    abstract::Transfection of a hamster cell mutant with human DNA corrected both the low-density lipoprotein receptor-deficient phenotype and the multiple glycosylation defects of the cells. Independently transfected colonies contained a small set of common human DNA fragments. These fragments may correspond to the human analog of...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kingsley DM,Sege RD,Kozarsky KF,Krieger M

    更新日期:1986-07-01 00:00:00

  • Developmental regulation of human gamma-globin genes in transgenic mice.

    abstract::We report results showing that several gamma gene promoter elements participate in the developmental control of gamma-globin genes. Four gamma gene constructs with 5' truncated at -141, -201, -382, and -730 of the A gamma gene promoter linked to a micro locus control region (microLCR) cassette were used for production...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Stamatoyannopoulos G,Josephson B,Zhang JW,Li Q

    更新日期:1993-12-01 00:00:00

  • Stabilization of tubulin mRNA by inhibition of protein synthesis in sea urchin embryos.

    abstract::An increased level of unpolymerized tubulin caused by depolymerization of microtubules in sea urchin larvae resulted in a rapid loss of tubulin mRNA, which was prevented by nearly complete inhibition of protein synthesis. Results of an RNA run-on assay indicated that inhibition of protein synthesis does not alter tubu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gong ZY,Brandhorst BP

    更新日期:1988-08-01 00:00:00

  • Ribosomal protein L7a is encoded by a gene (Surf-3) within the tightly clustered mouse surfeit locus.

    abstract::The mouse Surfeit locus, which contains a cluster of at least four genes (Surf-1 to Surf-4), is unusual in that adjacent genes are separated by no more than 73 base pairs (bp). The heterogeneous 5' ends of Surf-1 and Surf-2 are separated by only 15 to 73 bp, the 3' ends of Surf-1 and Surf-3 are only 70 bp apart, and t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Giallongo A,Yon J,Fried M

    更新日期:1989-01-01 00:00:00

  • Dimerization in vivo and inhibition of the nonreceptor form of protein tyrosine phosphatase epsilon.

    abstract::cyt-PTP epsilon is a naturally occurring nonreceptor form of the receptor-type protein tyrosine phosphatase (PTP) epsilon. As such, cyt-PTP epsilon enables analysis of phosphatase regulation in the absence of extracellular domains, which participate in dimerization and inactivation of the receptor-type phosphatases re...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Toledano-Katchalski H,Tiran Z,Sines T,Shani G,Granot-Attas S,den Hertog J,Elson A

    更新日期:2003-08-01 00:00:00

  • Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break.

    abstract::Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence of R...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Signon L,Malkova A,Naylor ML,Klein H,Haber JE

    更新日期:2001-03-01 00:00:00

  • Binding of myc proteins to canonical and noncanonical DNA sequences.

    abstract::Using an in vitro binding-site selection assay, we have demonstrated that c-Myc-Max complexes bind not only to canonical CACGTG or CATGTG motifs that are flanked by variable sequences but also to noncanonical sites that consist of an internal CG or TG dinucleotide in the context of particular variations in the CA--TG ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Blackwell TK,Huang J,Ma A,Kretzner L,Alt FW,Eisenman RN,Weintraub H

    更新日期:1993-09-01 00:00:00

  • Id helix-loop-helix proteins antagonize pax transcription factor activity by inhibiting DNA binding.

    abstract::The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. The major mechanism by which Id proteins are thought to inhibit differentiation is through interaction with other HLH proteins and inhibition of their DNA-binding activity. Howe...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Roberts EC,Deed RW,Inoue T,Norton JD,Sharrocks AD

    更新日期:2001-01-01 00:00:00

  • The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.

    abstract::Human MED26 was originally purified in the cofactor required for the Sp1 activation complex (CRSP) as a 70-kDa component named CRSP70. This polypeptide was specific to metazoans and the “small” form of the Mediator complex. We report here that a Drosophila melanogaster homologue of MED26 similarly interacts with other...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Marr SK,Lis JT,Treisman JE,Marr MT 2nd

    更新日期:2014-07-01 00:00:00

  • The SANT domain of p400 ATPase represses acetyltransferase activity and coactivator function of TIP60 in basal p21 gene expression.

    abstract::The TIP60 histone acetyltransferase plays diverse roles in DNA damage responses, DNA double-strand break repair, and transcriptional regulation. TIP60 resides within a multisubunit complex that has been shown to be targeted by transcription factors and to be involved in histone acetylation and transcriptional activati...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Park JH,Sun XJ,Roeder RG

    更新日期:2010-06-01 00:00:00

  • Specific acetylation of chromosomal protein HMG-17 by PCAF alters its interaction with nucleosomes.

    abstract::Nonhistone chromosomal proteins HMG-14 and HMG-17 are closely related nucleosomal binding proteins that unfold the higher-order chromatin structure, thereby enhancing the transcription and replication potential of chromatin. Here we report that PCAF, a transcription coactivator with intrinsic histone acetyltransferase...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Herrera JE,Sakaguchi K,Bergel M,Trieschmann L,Nakatani Y,Bustin M

    更新日期:1999-05-01 00:00:00

  • Role of distinct mitogen-activated protein kinase pathways and cooperation between Ets-2, ATF-2, and Jun family members in human urokinase-type plasminogen activator gene induction by interleukin-1 and tetradecanoyl phorbol acetate.

    abstract::We have investigated the in vivo and in vitro regulation of the human urokinase-type plasminogen activator (uPA) gene by interleukin-1 (IL-1) and analyzed the transcription factors and signalling pathways involved in the response of the -2.0-kb uPA enhancer to IL-1 induction and to tetradecanoyl phorbol acetate (TPA) ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Cirillo G,Casalino L,Vallone D,Caracciolo A,De Cesare D,Verde P

    更新日期:1999-09-01 00:00:00

  • Characterization of novel parent-specific epigenetic modifications upstream of the imprinted mouse H19 gene.

    abstract::Genomic imprinting results in parent-specific monoallelic expression of a small number of genes in mammals. The identity of imprints is unknown, but much evidence points to a role for DNA methylation. The maternal alleles of the imprinted H19 gene are active and hypomethylated; the paternal alleles are inactive and hy...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Szabó PE,Pfeifer GP,Mann JR

    更新日期:1998-11-01 00:00:00