Abstract:
BACKGROUND:Monocarboxylate transporters (MCTs) mediate lactate transport across the plasma membrane of cells. The molecular mechanisms regulating monocarboxylate transport in smooth muscle cells (SMCs) remain poorly characterized. The aim of this study was to investigate the effects of DNA methylation on MCT expression and lactate transport in SMCs in relation to atherosclerosis. METHODS AND RESULTS:MCT expression was determined by real-time reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry in SMCs isolated from human aortas and coronary arteries. Bisulfite sequencing and confocal microscopic analysis were used to study DNA methylation and lactate transport in SMCs, respectively. Downregulation of MCT3 and impaired lactate transport were detected in proliferating/synthetic SMCs, relative to the contractile phenotype. A passage number- and atherosclerotic lesion-dependent methylation pattern of MCT3 was demonstrated in the CpG island located in exon 2. Treatment of SMCs with the demethylating agent 5-aza-2'-deoxycytidine restored MCT3 expression and normalized lactate transport. Furthermore, small interfering RNA-mediated specific MCT3 knockdown substantially stimulated SMC proliferation. CONCLUSIONS:These data indicate that DNA methylation may modify monocarboxylate transport by suppressing MCT3 expression, which could be important in regulating SMC function and the development of atherosclerosis.
journal_name
Circulationjournal_title
Circulationauthors
Zhu S,Goldschmidt-Clermont PJ,Dong Cdoi
10.1161/CIRCULATIONAHA.104.519025subject
Has Abstractpub_date
2005-08-30 00:00:00pages
1353-61issue
9eissn
0009-7322issn
1524-4539pii
CIRCULATIONAHA.104.519025journal_volume
112pub_type
杂志文章相关文献
CIRCULATION文献大全abstract:BACKGROUND:Cardiopulmonary bypass and cardiac surgery are associated with a significant systemic inflammatory response that may increase postoperative complications. This meta-analysis assessed whether the benefits and risks of corticosteroid use were dose dependent in adult cardiac surgery. METHODS AND RESULTS:Random...
journal_title:Circulation
pub_type: 杂志文章,meta分析
doi:10.1161/CIRCULATIONAHA.108.848218
更新日期:2009-04-14 00:00:00
abstract:BACKGROUND:High C-reactive protein (CRP) levels are associated with an increased risk of cardiovascular events, even in apparently healthy individuals. It has not been established whether elevated CRP reflects an increased burden of subclinical coronary atherosclerosis. METHODS AND RESULTS:We studied a stratified rand...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.0000032135.98011.c4
更新日期:2002-09-03 00:00:00
abstract:BACKGROUND:Atherosclerosis is a multifactorial disease in which inflammatory processes play an important role. Inflammation underlies lesion evolution at all stages, from establishment to plaque rupture and thrombosis. Costimulatory molecules of the tumor necrosis factor superfamily such as CD40/CD40L and OX40/OX40L ha...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.107.699173
更新日期:2008-03-11 00:00:00
abstract:BACKGROUND:Resting cardiac function does not necessarily affect exercise capacity. However, to determine whether it affects early dynamics of oxygen uptake (VO2) during exercise, we measured VO2 during a constant work rate and during incremental exercise testing in patients with a history of myocardial infarction. VO2 ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.90.5.2324
更新日期:1994-11-01 00:00:00
abstract:BACKGROUND:Radiofrequency ablation of type 1 atrial flutter (AF1) has recently evolved toward an anatomically guided procedure directed to isthmuses at the lower part of the right atrium (RA). However, different types of block at these isthmuses may be observed and potentially correlated with different late outcomes. I...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.94.12.3204
更新日期:1996-12-15 00:00:00
abstract:BACKGROUND:Cardiac growth and function may be modulated in part by trophic effects of neurohormones. Specifically, aldosterone has been shown to stimulate the growth of cardiac myocytes and the accumulation of cardiac extracellular matrix proteins. Moreover, a variant of the aldosterone synthase gene (a cytosine/thymid...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.99.17.2255
更新日期:1999-05-04 00:00:00
abstract:BACKGROUND:Serum uric acid (UA) could be a valid prognostic marker and useful for metabolic, hemodynamic, and functional (MFH) staging in chronic heart failure (CHF). METHODS AND RESULTS:For the derivation study, 112 patients with CHF (age 59+/-12 years, peak oxygen consumption [Vo2] 17+/-7 mL/kg per minute) were recr...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000065637.10517.A0
更新日期:2003-04-22 00:00:00
abstract:BACKGROUND:Elevated asymmetrical dimethylarginine (ADMA) is a novel risk factor for atherosclerosis that may impair endothelial function by interfering with endothelial nitric oxide synthesis. To gain insight into the effects of ADMA on systemic endothelial function, we examined the association between ADMA and brachia...
journal_title:Circulation
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCULATIONAHA.107.690016
更新日期:2007-09-18 00:00:00
abstract:BACKGROUND:Administration of protease inhibitors (PIs) to HIV-infected individuals has been associated with hyperlipidemia. In this study, we characterized the lipoprotein profile in subjects receiving ritonavir, indinavir, or nelfinavir, alone or in combination with saquinavir. METHODS AND RESULTS:Plasma lipoprotein ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.100.7.700
更新日期:1999-08-17 00:00:00
abstract:BACKGROUND:For patients with in-hospital cardiac arrests attributable to nonshockable rhythms, delays in epinephrine administration beyond 5 minutes is associated with worse survival. However, the extent of hospital variation in delayed epinephrine administration and its effect on hospital-level outcomes is unknown. M...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.116.025459
更新日期:2016-12-20 00:00:00
abstract::Optimal electrode position for long-term transvenous cardiac pacing is in the apex of the right ventricle. Intracardiac electrograms, surface electrocardiograms, frontal fluoroscopy, lateral roentgenograms, and pacing threshold levels were studied in two groups of patients: One group was comprised of five patients wit...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.42.4.701
更新日期:1970-10-01 00:00:00
abstract::Postoperative angiographic studies were carried out at 1 month, 1 year, and 10 years in two groups of patients: 238 patients with saphenous vein (SV) grafts and 40 patients with internal mammary artery (IMA) grafts. Cumulative patency was better in IMA grafts, both at 1 year (88.5% vs 76.4%) and at 10 years (84.1% vs ...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Digital vasospastic phenomena have been reported to result from use of nonselective and cardioselective beta-adrenoreceptor-blocking drugs. The effects of 80 mg/day propranolol and 100 mg/day metoprolol on finger hemodynamics and clinical responses were compared with those of placebo in 16 patients with Raynaud's phen...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.72.3.466
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND:Cardiac arrest without evident cardiac disease may be caused by subclinical genetic conditions. Provocative testing to unmask a phenotype is often necessary to detect primary electrical disease, direct genetic testing, and perform family screening. METHODS AND RESULTS:Patients with apparently unexplained ca...
journal_title:Circulation
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCULATIONAHA.109.853143
更新日期:2009-07-28 00:00:00
abstract:BACKGROUND:We report the single-institution, long-term results of 358 patients with simple transposition of the great arteries surviving >30 days after a Mustard (n=226, 1965 to 1980) or Senning (n=132, 1978 to 1992) procedure. METHODS AND RESULTS:Outcome measures included late death, reintervention, ECG and ambulator...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.100.suppl_2.ii-176
更新日期:1999-11-09 00:00:00
abstract:BACKGROUND:Fibrinogen has been identified as an independent risk factor for cardiovascular disease and associated with traditional cardiovascular risk factors. Also, the role of elevated fibrinogen in thrombosis suggests that it may be on the causal pathway for certain risk factors to exert their effect. These associat...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.14.1634
更新日期:2000-10-03 00:00:00
abstract:BACKGROUND:Long-term beta-adrenergic blockade does not appear to be associated with drug-induced training in patients with congestive heart failure (CHF); whether exercise training can increase peak aerobic capacity in patients with CHF who are treated with beta-adrenergic blockers is currently unknown. METHODS AND RE...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/01.cir.95.7.1764
更新日期:1997-04-01 00:00:00
abstract:BACKGROUND:Pharmacological ventricular rate control is an acceptable atrial fibrillation (AF) therapy limited by systemic toxicity. We postulate that focal catheter-based drug delivery into the atrioventricular nodal (AVN) region may effectively control ventricular rate during AF without systemic toxicity. This study e...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.105.609420
更新日期:2006-05-23 00:00:00
abstract::This is the first scientific statement from the American Heart Association on maternal resuscitation. This document will provide readers with up-to-date and comprehensive information, guidelines, and recommendations for all aspects of maternal resuscitation. Maternal resuscitation is an acute event that involves many ...
journal_title:Circulation
pub_type: 杂志文章,实务指引,评审
doi:10.1161/CIR.0000000000000300
更新日期:2015-11-03 00:00:00
abstract:BACKGROUND:Anisotropic conduction properties may provide a substrate for reentrant arrhythmias. We investigated the age-dependent changes of structural and functional anisotropy in isolated right atria from infant (1 to 2 months), young (6 to 12 months), and old (6 to 10 years) dogs. METHODS AND RESULTS:The histology ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.0000015506.36371.0d
更新日期:2002-04-30 00:00:00
abstract:BACKGROUND:Chronic pulmonary regurgitation after repair of tetralogy of Fallot (TOF) may lead to right ventricular dilatation, which may be accompanied by ventricular tachycardia and sudden death. We aimed to examine the effects of pulmonary valve replacement (PVR) on (1) certain electrocardiographic markers predictive...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.103.20.2489
更新日期:2001-05-22 00:00:00
abstract:BACKGROUND:In contrast to systolic function, which is relatively well preserved with advancing age, diastolic function declines steadily after age 30. Our goal was to determine whether changes in diastolic function that occur with aging could be reversed with exercise training. Methods and Results-- Adult (6-month-old)...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.104.2.221
更新日期:2001-07-10 00:00:00
abstract:BACKGROUND:Inflammatory vasculopathies, ranging from the vasculitides (Takayasu arteritis, giant cell arteritis, and polyarteritis nodosa) to atherosclerosis, display remarkable target tissue tropisms for selected vascular beds. Molecular mechanisms directing wall inflammation to restricted anatomic sites within the va...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.108.789172
更新日期:2008-09-16 00:00:00
abstract:BACKGROUND:We investigated the feasibility of bioluminescence imaging (BLI) for the in vivo assessment of cardiac allograft viability and visualization of passenger leukocytes during the course of acute rejection. METHODS AND RESULTS:Hearts of FVB (H-2q) luciferase-green fluorescent protein transgenic mice (beta-actin...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.104.524777
更新日期:2005-08-30 00:00:00
abstract:BACKGROUND:Few published data describe long-term survival of dialysis patients undergoing surgical versus percutaneous coronary revascularization in the era of drug-eluting stents (DES). METHODS AND RESULTS:Using United States Renal Data System data, we identified 23 033 dialysis patients who underwent coronary revasc...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.112.001264
更新日期:2013-05-07 00:00:00
abstract::South Asians (from Bangladesh, Bhutan, India, the Maldives, Nepal, Pakistan, and Sri Lanka) make up one quarter of the world's population and are one of the fastest-growing ethnic groups in the United States. Although native South Asians share genetic and cultural risk factors with South Asians abroad, South Asians in...
journal_title:Circulation
pub_type: 杂志文章,实务指引,评审
doi:10.1161/CIR.0000000000000580
更新日期:2018-07-03 00:00:00
abstract:BACKGROUND:The incidence and cause of sudden cardiac death (SCD) in athletes is debated with hypertrophic cardiomyopathy often reported as the most common cause. METHODS AND RESULTS:A database of all National Collegiate Athletic Association deaths (2003-2013) was developed. Additional information and autopsy reports w...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:10.1161/CIRCULATIONAHA.115.015431
更新日期:2015-07-07 00:00:00
abstract::To assess the effects of angioplasty (PTCA) and intracoronary streptokinase (ICSK) on relative myocardial perfusion, we administered 99mTc-macroaggregated albumin (MAA) to the uninvolved coronary artery before successful PTCA in 33 patients and before successful infusion of ICSK in eight patients and of 111In-MAA into...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.72.2.334
更新日期:1985-08-01 00:00:00
abstract::An experimental approach is described to quantitate inhomogeneity in extracellular K concentration ([K+]out) in the presence of ischemia and to relate this inhomogeneity to the electrophysiologic changes. Extracellular potassium concentration and local direct-current electrograms from the same sites were measured in i...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.77.5.1125
更新日期:1988-05-01 00:00:00
abstract:BACKGROUND:Biomarkers that predict response to cardiac resynchronization therapy (CRT) in heart failure patients with dyssynchrony (HFDYS) would be clinically important. Circulating extracellular microRNAs (miRNAs) have emerged as novel biomarkers that may also play important functional roles, but their relevance as ma...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.114.013220
更新日期:2015-06-23 00:00:00