cDNA clone to chick corneal chondroitin/dermatan sulfate proteoglycan reveals identity to decorin.

abstract：:Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence wa...

journal_title：Archives of biochemistry and biophysics

authors： Shen P,Howlett GJ

更新日期：1992-09-01 00:00:00

abstract：:Endothelial senescence is the main risk factor that contributes to vascular dysfunction and the progression of vascular disease. Carbon monoxide (CO) plays an important role in preventing vascular dysfunction and in maintaining vascular physiology or homeostasis. The application of exogenous CO has been shown to confe...

journal_title：Archives of biochemistry and biophysics

authors： Zheng M,Chen Y,Park J,Song HC,Chen Y,Park JW,Joe Y,Chung HT

更新日期：2019-11-30 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:We previously reported that administration of dexamethasone (DEX) and other selected pharmacological agents to rats resulted in a profound increase in hepatic cytochrome P450 3A1 in both sexes, but male constitutive P450 3A2 was modestly increased (4-fold) in adult males and not detected in either treated or untreated...

journal_title：Archives of biochemistry and biophysics

authors： Ghosal A,Sadrieh N,Reik L,Levin W,Thomas PE

更新日期：1996-08-01 00:00:00

abstract：:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and i...

journal_title：Archives of biochemistry and biophysics

authors： Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E

更新日期：2011-05-01 00:00:00

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:Human sorcin, M(r) 22-kDa/pI 5.3, was identified in HOB1 lymphoma cells resistant to 1.0 microM vincristine and polyclonal antibody against it was produced. Using antibody to precipitate sorcin from 32P-labeled cell extract, the SDS-PAGE-resolved human sorcin was not phosphorylated in contrast to the report of A. M. v...

journal_title：Archives of biochemistry and biophysics

authors： Lee WP

更新日期：1996-01-15 00:00:00

abstract：:It has now been more than 20years since the vitamin D receptor was identified in cells of the immune system. The immune system has now been established as an important target of vitamin D. Vitamin D receptor knockout and vitamin D deficient mice have a surplus of effector T cells that have been implicated in the patho...

journal_title：Archives of biochemistry and biophysics

authors： Cantorna MT

更新日期：2012-07-01 00:00:00

abstract：:Mammalian mitochondria are sensitive targets of the cytotoxic effects of superoxide (O.2-) and nitric oxide (.NO). In turn, when superoxide and nitric oxide are simultaneously produced, they rapidly react with each other yielding the highly oxidizing peroxynitrite anion (ONOO-) which may be also toxic to mammalian mit...

journal_title：Archives of biochemistry and biophysics

authors： Radi R,Rodriguez M,Castro L,Telleri R

更新日期：1994-01-01 00:00:00

abstract：:Enzymes subjected to freeze-thawing are known to be protected by polymers that are preferentially excluded from the hydrated surface of proteins [reviewed in Carpenter et al. (1994) ACS Symp. Ser. 567, 134-147]. Preferentially excluded solutes are also known to stabilize quaternary structure, which enhances the thermo...

journal_title：Archives of biochemistry and biophysics

authors： Anchordoquy TJ,Carpenter JF

更新日期：1996-08-15 00:00:00

abstract：:CYP2D15 is the canine ortholog of human CYP2D6, the human CYP2D isoform involved in the metabolism of drugs such as antiarhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Similar to human, canine CYP2D15 is expressed in the liver, with detectable levels in several other tissues. Three different CYP2D...

journal_title：Archives of biochemistry and biophysics

authors： Roussel F,Duignan DB,Lawton MP,Obach RS,Strick CA,Tweedie DJ

更新日期：1998-09-01 00:00:00

abstract：:A 60- to 70-fold purification of an NAD+ glycohydrolase from the inner membrane of rat liver mitochondria to apparent homogeneity on sodium dodecyl sulfate (SDS)-polyacrylamide slab gel is described. The minimum molecular weight of the enzyme on polyacrylamide gels in the presence of SDS is around 62,000. The enzyme s...

journal_title：Archives of biochemistry and biophysics

authors： Moser B,Winterhalter KH,Richter C

更新日期：1983-07-01 00:00:00

abstract：:Using 31P NMR spectroscopy, we have measured the rate of ATP synthesis, and the free concentrations of ATP, ADP, cytoplasmic Pi, and H+ in maize root tips under a wide range of conditions. We show that the ratio [ATP]/[ADP] in normoxic root tips is greater than 25. We found no simple relationship between the concentra...

journal_title：Archives of biochemistry and biophysics

authors： Roberts JK,Lane AN,Clark RA,Nieman RH

更新日期：1985-08-01 00:00:00

abstract：:The mechanisms by which sterols and nonsterols regulate hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene expression was investigated by measuring mRNA, protein, and enzyme activity in rats fed cholesterol or given drugs that deplete both endogenous sterols and nonsterols or that selectively deple...

journal_title：Archives of biochemistry and biophysics

authors： Ness GC,Eales S,Lopez D,Zhao Z

更新日期：1994-02-01 00:00:00

abstract：:Interleukin (IL)-2 knockout (KO) mice, which spontaneously develop symptoms of inflammatory bowel disease similar to ulcerative colitis in humans, were made vitamin D deficient (D-) or vitamin D sufficient (D+) or were supplemented with 1,25-dihydroxyvitamin D(3) (1,25D3). 1,25-Dihydroxyvitamin D3 supplementation, but...

journal_title：Archives of biochemistry and biophysics

authors： Bemiss CJ,Mahon BD,Henry A,Weaver V,Cantorna MT

更新日期：2002-06-15 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) catalyzes the beta-elimination of ammonia from L-phenylalanine to trans-cinnamic acid. A study of inhibition of PAL by phenol, ortho-cresol, and meta-cresol gave mixed inhibition; para-cresol is not an inhibitor. The calculated values of K(i) and alphaK(i) are phenol, K(i)=2.1+/-0.5 m...

journal_title：Archives of biochemistry and biophysics

authors： Alunni S,Cipiciani A,Fioroni G,Ottavi L

更新日期：2003-04-15 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:The three cysteine residues per subunit of pig muscle phosphoglucose isomerase show different reactivities toward various sulfhydryl reagents. The organomercurial, p-mercuribenzoate, can titrate two of the sulfhydryl groups under nondenaturing conditions. 2,2'-Dithiodipyridine, 5,5'-dithiobis(2-nitrobenzoic acid), iod...

journal_title：Archives of biochemistry and biophysics

authors： Scott-Ennis RJ,Noltmann EA

更新日期：1985-05-15 00:00:00

abstract：:Recent X-ray crystal structures of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 in complex with amlodipine showed two bound ligand molecules, one in the active site and one in the substrate access channel. Based on the X-ray crystal structures, we investigated the interactions of P450 2B4 and 2B6 with amlo...

journal_title：Archives of biochemistry and biophysics

authors： Jang HH,Davydov DR,Lee GY,Yun CH,Halpert JR

更新日期：2014-03-01 00:00:00

abstract：:Conidiospores of wild type and two mutant strains of Neurospora crassa were grown on [3-13C]serine, [2-13C]glycine, or [13C]formate. Acid extracts of the mycelia were analyzed by 13C NMR for incorporation of the 13C label into choline, serine, and adenine. The goal was to elucidate the function of cytosolic serine hyd...

journal_title：Archives of biochemistry and biophysics

authors： Jeong SS,Schirch V

更新日期：1996-11-15 00:00:00

abstract：:All six available lysine residues in bacteriorhodopsin were amidinated with dimethyl-3,3'-dithiobispropionimidate, which is a crosslinking agent. The photocycle was studied by measuring light absorption and electric signals. The data show an essential change in the photocycle: instead of single components, the rise of...

journal_title：Archives of biochemistry and biophysics

authors： Tóth-Boconádi R,Taneva SG,Kiselev AV,Abdulaev NG,Keszthelyi L

更新日期：1988-02-01 00:00:00

abstract：:Juvenile hormone binding protein (JHBP) acts as a shuttle, carrying one of the most crucial hormones for insect development to target tissues. We have found that although the JHBP molecule does not contain tryptophan residues, it exhibits a weak fluorescence maximum near 420nm upon excitation at 315nm. Gel filtration ...

journal_title：Archives of biochemistry and biophysics

authors： Bystranowska D,Szewczuk Z,Lisowski M,Sitkiewicz E,Dobryszycki P,Ożyhar A,Kochman M

更新日期：2012-01-01 00:00:00

abstract：:(6R)-5,6,7,8-Tetrahydro-L-biopterin (R-THBP) is a cofactor not only for aromatic amino acid hydroxylases in mammalian tissues but also for nitric oxide synthase (NOS) induced by endotoxins or cytokines in some kinds of cells. Recently it has been reported that nitric oxide (NO) has biological activity in endothelium a...

journal_title：Archives of biochemistry and biophysics

authors： Nunokawa Y,Ishihara T,Kanai T,Noguchi T

更新日期：1992-11-01 00:00:00

abstract：:Accumulating evidence suggests that oxidative stress resulting in reactive oxygen species generation plays a pivotal role in neurodegenerative diseases, supporting the realization of the use of radical scavengers, metal chelator agents, such as the natural polyphenols for therapy. In this study, we have focused on spe...

journal_title：Archives of biochemistry and biophysics

authors： Weinreb O,Amit T,Youdim MB

更新日期：2008-08-15 00:00:00

abstract：:The primary site of action for the novel antibiotic, bicyclomycin, in Escherichia coli has been identified to be the rho transcription termination factor. The inhibition of rho poly(C)-stimulated hydrolysis of ATP by bicyclomycin has been found to proceed by a non-competitive, reversible pathway with respect to ATP (K...

journal_title：Archives of biochemistry and biophysics

authors： Park HG,Zhang X,Moon HS,Zwiefka A,Cox K,Gaskell SJ,Widger WR,Kohn H

更新日期：1995-11-10 00:00:00

abstract：:Angiotensin II (Ang II) plays an important role in inflammatory process. Acute lung injury (ALI), an inflammatory disorder of the lung, is commonly associated with endotoxemia; however, the mechanism that endotoxin (lipopolysaccharide, LPS) induces the inflammatory response in ALI is not well defined. Here, we showed,...

journal_title：Archives of biochemistry and biophysics

authors： Liu L,Qiu HB,Yang Y,Wang L,Ding HM,Li HP

更新日期：2009-01-01 00:00:00

abstract：:Stem cells maintain their self-renewal and multipotency capacities through a self-organizing network of transcription factors and intracellular pathways activated by extracellular signaling from the microenvironment or "niche" in which they reside in vivo. In the adult mammalian brain new neurons continue to be genera...

journal_title：Archives of biochemistry and biophysics

authors： Porlan E,Perez-Villalba A,Delgado AC,Ferrón SR

更新日期：2013-06-01 00:00:00

abstract：:p53, a tumor suppressor and transcription factor, is a critical modulator in the cellular response to stress. Exposure of glutathione-deficient GCS-2 cells to arsenite significantly phosphorylated and stabilized p53. In addition, p53 transcriptionally repressed Hsp90beta gene expression. Mutation analysis revealed a p...

journal_title：Archives of biochemistry and biophysics

authors： Habib GM

更新日期：2009-01-01 00:00:00

abstract：:Reductant-independent ATPase activity was initiated and studied for Azotobacter vinelandii and Clostridium pasteuranium nitrogenase proteins (Av1, Cp1 and Av2, Cp2, 1 designating the iron molybdenum protein and 2 the iron protein) and their heterologous crosses by two methods: (1) allowing dithionite to be depleted fr...

journal_title：Archives of biochemistry and biophysics

authors： Larsen C,Christensen S,Watt GD

更新日期：1995-11-10 00:00:00