The effects of isoflurane on airway smooth muscle crossbridge kinetics in Fisher and Lewis rats.

Abstract:

:Our aim was to determine how isoflurane modified crossbridge (CB) number and kinetics in airway smooth muscle (ASM) and to compare its effects in Fisher and Lewis rats, two strains with differences in airway responsiveness. The effects of isoflurane (2 MAC) on isotonic and isometric contractility in tracheal ASM strips were investigated after methacholine (10(-6) M)-induced contraction. CB mechanics and kinetics were analyzed using the formalism of Huxley's equations adapted to ASM. After isoflurane, maximum velocity did not differ from baseline in Lewis rats, whereas it was significantly less than baseline in Fisher rats ( approximately 25%), the most reactive strain. Isoflurane totally reversed methacholine-induced increase in active CB number in Lewis rats (2.4 +/- 0.5 versus 1.8 +/- 0.4 10(9)/mm(2) after methacholine and isoflurane, respectively) whereas reversal was only partial in Fisher rats (2.7 +/- 0.4 versus 2.1 +/- 0.3 10(9)/mm(2) after methacholine and isoflurane, respectively). Isoflurane induced a 40% increase in attachment step duration in both strains and an almost twofold increase in the CB cycle duration compared with baseline in Lewis rats. The isoflurane-induced increase in detachment step duration was less in Lewis than in Fisher rats (P < 0.05). We concluded that isoflurane modulated CB number and CB cycling rates of isolated rat ASM differently depending on the level of airway responsiveness.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Duracher C,Blanc FX,Gueugniaud PY,David JS,Riou B,Lecarpentier Y,Coirault C

doi

10.1213/01.ANE.0000152644.44927.E4

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

136-42, table of contents

issue

1

eissn

0003-2999

issn

1526-7598

pii

101/1/136

journal_volume

101

pub_type

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