Abstract:
STUDY OBJECTIVE:In these days, it was reported that bone marrow (BM) cells might take part in the remodeling of some systemic vascular diseases; however, it remains unknown whether the BM cells were involved in the vascular remodeling of pulmonary arteries and the progression of pulmonary hypertension (PH). The purpose of this study was to investigate whether BM-derived cells contribute to pulmonary vascular remodeling in hypoxia-induced PH. MATERIALS AND METHODS:To investigate the role of BM-derived cells, we transplanted the whole BM of enhanced green fluorescent protein (GFP)-transgenic mice to the lethally irradiated syngeneic mice (n = 30). After 8 weeks, chimera mice were exposed to consistent hypoxia using a hypoxic chamber (10% O(2)) for up to 4 or 8 weeks (10 mice per group). After hemodynamics and the ratio of right ventricular (RV) weight to left ventricle (LV) weight, RV/(LV + septum [S]), were measured, histologic and immunofluorescent staining were performed. RESULTS:BM-transplanted mice showed a high chimerism (mean [+/- SEM], 91 +/- 2.3%). RV systolic pressure and the RV/(LV + S) ratio increased significantly with time in PH mice, indicating RV hypertrophy. Marked vascular remodeling including medial hypertrophy and adventitial proliferation was observed in the pulmonary arteries of PH mice. Strikingly, a number of GFP(+) cells were observed at the pulmonary arterial wall, including the adventitia, in hypoxia-induced PH mice, while very few cells were observed in the control mice. Metaspectrometer measurements using confocal laser scanning microscopy confirmed that this green fluorescence was produced by GFP, suggesting that these GFP(+) cells were mobilized from the BM. Most of them expressed alpha-smooth muscle actin, a smooth muscle cell, or myofibroblast phenotype, and contributed to the pulmonary vascular remodeling. A semiquantitative polymerase chain reaction of the GFP gene revealed that the BM-derived GFP-positive cells in the PH group were observed more than eightfold as often compared with the control mice. CONCLUSION:The BM-derived cells mobilize to the hypertensive pulmonary arteries and contribute to the pulmonary vascular remodeling in hypoxia-induced PH mice.
journal_name
Chestjournal_title
Chestauthors
Hayashida K,Fujita J,Miyake Y,Kawada H,Ando K,Ogawa S,Fukuda Kdoi
10.1378/chest.127.5.1793subject
Has Abstractpub_date
2005-05-01 00:00:00pages
1793-8issue
5eissn
0012-3692issn
1931-3543pii
S0012-3692(15)34751-6journal_volume
127pub_type
杂志文章相关文献
CHEST文献大全abstract:BACKGROUND:Increased expression of the human epididymis protein 4 (HE4) was previously described in lung biopsy samples from patients with cystic fibrosis (CF). It remains unknown, however, whether serum HE4 concentrations are elevated in CF. METHODS:Seventy-seven children with CF from six Hungarian CF centers and 57 ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1016/j.chest.2016.04.006
更新日期:2016-09-01 00:00:00
abstract:INTRODUCTION:Gastroesophageal reflux has been suggested as an underlying cause of chronic lung disease. The aim of this study was to assess the value of pepsin and bile acids, both components of GI secretions, in the lungs of children with chronic lung diseases as possible markers for gastroesophageal reflux disease an...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.07-0316
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:Pathogenic bacteria colonize the airways of 30% to 40% of patients with COPD and cause approximately 50% of exacerbations. New strains of nontypeable Haemophilus influenzae (NTHI) and Moraxella catarrhalis are associated with exacerbations. Antimicrobial protein/peptides (AMPs) play important roles in innate...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.10-2760
更新日期:2011-09-01 00:00:00
abstract::A young man presented to us with an asymptomatic ovoid mass which appeared to be in the right major fissure. The roentgenographic appearance of the lesion was that of a pseudotumor. The computerized tomography scan suggested tissue density leading to exploration. At surgery, we found the mass to be a portion of liver ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.78.2.346
更新日期:1980-08-01 00:00:00
abstract::Disputes regarding life-prolonging treatments are stressful for all parties involved. These disagreements are appropriately almost always resolved with intensive communication and negotiation. Those rare cases that are not require a resolution process that ensures fairness and due process. We describe three recent cas...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1016/j.chest.2016.05.026
更新日期:2016-09-01 00:00:00
abstract:STUDY OBJECTIVES:COPD patients run a risk of developing nocturnal oxygen desaturation. When evaluating patients with nocturnal hypoxemia, an unfamiliar hospital environment and the monitoring equipment may cause sleep disturbances. It was hypothesized that increased sleep disruption will lead to fewer instances of desa...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.119.6.1820
更新日期:2001-06-01 00:00:00
abstract:STUDY OBJECTIVES:To determine whether the nuclear accumulation of p53 in patients with early bronchial neoplasia represents an altered susceptibility for the development of lung cancer. PATIENTS AND MEASUREMENTS:We evaluated the percentage of cells accumulating nuclear p53 immunohistochemically in squamous cell carcin...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.123.1.181
更新日期:2003-01-01 00:00:00
abstract::We evaluated the physiologic effects of pressure support ventilation by nasal route (NPSV) in eight patients with severe stable COPD and chronic hypercapnia who were randomly submitted to 2-h sessions of NPSV both with a portable ventilator (Respironics BIPAP device) and with a standard ventilator (Bird 6400ST device)...
journal_title:Chest
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1378/chest.101.2.385
更新日期:1992-02-01 00:00:00
abstract::There is a general consensus that antimicrobial resistance in the hospital setting has emerged as an important variable influencing patient outcome and resource utilization. Hospitals worldwide are faced with increasingly rapid emergence and spread of antibiotic-resistant bacteria. Both antibiotic-resistant Gram-negat...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.123.5_suppl.464s
更新日期:2003-05-01 00:00:00
abstract::The clinical and histopathologic features of pulmonary amyloidosis were reviewed in 22 patients with systemic amyloidosis who came to autopsy. Eleven of 12 patients (92 percent) with primary amyloidosis had prominent interalveolar amyloid deposits. Symptoms attributable to these deposits were found in four cases (33 p...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.74.5.543
更新日期:1978-11-01 00:00:00
abstract:STUDY OBJECTIVE:Laboratory testing plays a minor role in the assessment of aortic dissection. Its main value is in the exclusion of other diseases. Following an incidental observation, we systematically investigated the relationship between elevated d-dimer levels and acute aortic dissection. DESIGN:We prospectively t...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.123.5.1375
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Clinical staging of non-small cell lung cancer (NSCLC) helps determine the prognosis and treatment of patients; few data exist on the accuracy of clinical staging and the impact on treatment and survival of patients. We assessed whether participant or trial characteristics were associated with clinical stagi...
journal_title:Chest
pub_type: 杂志文章,meta分析
doi:10.1016/j.chest.2018.10.020
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:To determine the contribution of the various components of a rehabilitation program to the improvement of exercise capacity and health-related quality of life (HRQL) in patients with COPD. DATA SOURCES:MEDLINE (1966 to April 1996) was searched. Abstracts presented at international conferences were also hand ...
journal_title:Chest
pub_type: 杂志文章,meta分析
doi:10.1378/chest.111.4.1077
更新日期:1997-04-01 00:00:00
abstract::Human leukocyte antigen (HLA) tissue-typing studies on patients with genetically transmitted hypertrophic cardiomyopathy have demonstrated an HLA linkage in a Caucasian and black patient group and an HLA-DR locus association in a Japanese population sample. To confirm whether a specific HLA antigen(s) might serve as a...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.81.4.466
更新日期:1982-04-01 00:00:00
abstract::A case of idiopathic pulmonary hypertension is associated with portal venous thrombosis. The patient's chest roentgenogram revealed numerous nodular densities which at necropsy were found to have been caused by an exuberant fibroblastic reaction around small pulmonary arterioles. This is an example of the unusual asso...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.78.1.111
更新日期:1980-07-01 00:00:00
abstract::This study evaluated the influence of pregnancy as a relapse factor in pulmonary tuberculosis in 215 patients who recieved adequate chemotherapy. A comparison was made between the evolution of patients who had pregnancies and those who did not. On ending the period of observation (details are lacking with respect to o...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.68.6.800
更新日期:1975-12-01 00:00:00
abstract::Morbid obesity is often associated with severe respiratory insufficiency, commonly known as the pickwickian syndrome. This can be divided into the following two primary breathing disorders which can affect patients alone or in combination: the obstructive sleep apnea syndrome (SAS); and the obesity-hypoventilation syn...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.90.1.81
更新日期:1986-07-01 00:00:00
abstract:STUDY OBJECTIVE:This study aimed to determine the differences between ciliary beat frequencies of respiratory ciliated cells from peripheral bronchioles and from proximal bronchi in humans. DESIGN:Measurements were made from resected lungs. Ciliated cells were harvested by brushing the mucosa of each site immediately ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.111.3.692
更新日期:1997-03-01 00:00:00
abstract::A five-year clinical follow-up and the results of myocardial biopsies are described in a patient with primary restrictive cardiomyopathy. Histologic examination of a right ventricular endomyocardial biopsy taken early in the course of the illness was not contributory. Histologic examination of a left ventricular endom...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.84.2.236
更新日期:1983-08-01 00:00:00
abstract::Fifteen patients in the early stages of the adult respiratory distress syndrome with severe hypoxemia who were capable of maintaining adequate spontaneous ventilation were treated with continuous positive airway pressure (CPAP). The optimal level of CPAP was adjusted for each patient to achieve the highest oxygenation...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.76.3.257
更新日期:1979-09-01 00:00:00
abstract::Random assessments of SaO2 were performed via pulse oximetry in 274 hospitalized non-ICU patients prescribed supplemental O2 in a large tertiary care university hospital. In 507 assessments performed in patients inspiring the prescribed O2, 426 were receiving excessive amounts of O2 to maintain a SaO2 > or = 92 percen...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.102.6.1672
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:The implementation of workplace smoking bans has contributed to a significant reduction in the incidence of acute coronary syndrome admissions, but their influence on adult acute pulmonary disease admissions is unclear. We sought to assess the impact of a national smoking ban on nationwide admissions of indi...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.11-2757
更新日期:2012-09-01 00:00:00
abstract:OBJECTIVE:Congenital airway malacia is one of the few causes of irreversible airways obstruction in children, but the incidence in the general population is unknown. Severe airway malacia or malacia associated with specific syndromes is usually recognized and diagnosed early in infancy, but information about clinical f...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.128.5.3391
更新日期:2005-11-01 00:00:00
abstract::A 77-year-old man with a history of renal cell carcinoma presented with orthostatic syncope. Investigation revealed metastatic carcinoma in the left lung extending through the pulmonary veins into the left atrium obstructing the mitral orifice. On review of the literature, this case appears to be the first to describe...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.98.2.481
更新日期:1990-08-01 00:00:00
abstract:CASE PRESENTATION:A 78-year-old man with asthma and COPD presented with shortness of breath, cough, and severe malaise for 4 days. Upon arrival, the patient was conscious and body temperature was 37.5°C. Arterial oxygen saturation (Spo2) was 80% on room air. Laboratory data demonstrated a WBC count of 17,400/μL (89.5% ...
journal_title:Chest
pub_type: 杂志文章
doi:10.1016/j.chest.2019.07.034
更新日期:2020-01-01 00:00:00
abstract:STUDY OBJECTIVE:To explore the causes of sudden and unexpected death in a young and healthy military population, to examine the various patterns of diseases associated with these tragic events, and to investigate the factors found to be associated with this grave outcome. DESIGN:We retrospectively investigated 151 cas...
journal_title:Chest
pub_type: 杂志文章
doi:10.1378/chest.126.2.528
更新日期:2004-08-01 00:00:00
abstract:BACKGROUND:Acetazolamide and medroxyprogesterone acetate (MPA) are two respiratory stimulants that can be used in patients with stable hypercapnic COPD. DESIGN AND METHODS:The effects of acetazolamide, 250 mg bid, and MPA, 30 mg bid, on daytime and nighttime blood gas values and the influences on the hypercapnic and h...
journal_title:Chest
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1378/chest.123.5.1450
更新日期:2003-05-01 00:00:00
abstract::Sepsis is among the most common reasons for admission to ICUs throughout the world, and it is believed to be the third most common cause of death in the United States. The pathogenetic mechanism and physiologic changes associated with sepsis are exceedingly complex, but our understanding is evolving rapidly. The major...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.13-2104
更新日期:2014-06-01 00:00:00
abstract::Relapsing polychondritis (RP) is a rare disease characterized by recurrent inflammation and destruction of the cartilaginous structures. Tracheobronchial chondritis is a dreaded complication of RP. We wish to report a case of RP of the trachea and bronchi which was treated with nasal continuous positive airway pressur...
journal_title:Chest
pub_type: 杂志文章,评审
doi:10.1378/chest.112.6.1701
更新日期:1997-12-01 00:00:00
abstract:RATIONALE:Malnutrition in patients with COPD is associated with an impaired pulmonary status, reduced diaphragmatic mass, lower exercise capacity, and higher mortality rate when compared to adequately nourished individuals with COPD. Nutritional support may therefore be a useful part of their comprehensive care. PURPO...
journal_title:Chest
pub_type: 临床试验,杂志文章,meta分析,随机对照试验
doi:10.1378/chest.117.3.672
更新日期:2000-03-01 00:00:00